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All Journal Jurnal Ilmu Pertanian Indonesia Biotropika Prosiding Seminar Biologi Indonesian Journal of Biotechnology BIOLINK (Jurnal Biologi Lingkungan, Industri, Kesehatan) Jurnal Biolokus: Jurnal Penelitian Pendidikan Biologi dan Biologi AL-HAYAT: Journal of Biology and Applied Biology Floribunda Canrea Journal: Food Technology, Nutritions, and Culinary Journal Walisongo Journal of Information Technology Berkala Ilmiah Biologi Buitenzorg: Journal of Tropical Science
Arnia Sari Mukaromah
Departement Of Biology, Faculty Of Science And Technology, Universitas Islam Negeri Walisongo Semarang
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Earth & Planetary Sciences Education Engineering Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Veterinary

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Isolasi DNA tumbuhan hasil eksplorasi di Nusakambangan dengan metode kit di Laboratorium Treub, Kebun Raya Bogor Devi Octavia; Arnia Sari Mukaromah; Irfan Martiansyah; Mimin Mimin; S Ma'mun; Herman Rukmanto
Prosiding Seminar Biologi Vol 7 No 1 (2021): PROSIDING BIOLOGI ACHIEVING THE SUSTAINABLE DEVELOPMENT GOALS WITH BIODIVERSITY I
Publisher : Jurusan Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/psb.v7i1.24513

Abstract

Konservasi genetik merupakan suatu tindakan untuk menjaga, mendokumentasikan dan mengarsipkan spesimen tertentu dalam bentuk koleksi hidup maupun koleksi genom. Pendekatan molekuler sebagai alternatif dalam konservasi genetik, umumnya dilakukan dengan mengisolasi organ tertentu hingga diperoleh materi genetiknya. Tujuan penulisan artikel ini adalah untuk melaporkan hasil isolasi DNA dari beberapa sampel tumbuhan yang terdapat di Laboratorium Treub, Kebun Raya Bogor. Isolasi DNA mendukung metode konservasi genetik dari koleksi herbarium dan Bank Biji di Kebun Raya Bogor yang sudah ada sebelumnya. Metode isolasi DNA yang dilakukan berbasis pada penggunaan 2 kit DNA khusus untuk tumbuhan dengan protokol baku pada masing-masing kit. Adapun hasil isolasi DNA dari 32 sampel menunjukkan bahwa pita DNA yang muncul menjadi tanda dari keberhasilan konservasi genetik secara molekuler. Dari 29 yang diisolasi menggunakan Kit #1. Hanya 1 sampel yang muncul pita DNA. Kemudian 5 sampel lainnya berhasil terisolasi menggunakan Kit #2. Dari 32 sampel yang diisolasi, 13 sampel muncul pita dan 19 (tidak muncul pita) dengan variasi konsentrasi DNA. Hal tersebut diasumsikan beberapa kesalahan teknis penanganan dalam proses laboratorium.
Determination of allelopathic potential in mahogany (Swietenia macrophylla King) leaf litter using sandwich method Arnia Sari Mukaromah; Yekti Asih Purwestri; Yoshiharu Fujii
Indonesian Journal of Biotechnology Vol 21, No 2 (2016)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1758.807 KB) | DOI: 10.22146/ijbiotech.16456

Abstract

The sandwich method is a reliable screening bioassay that can be utilized to investigate allelopathic activity of leaf litter leachates. Screening the allelopathic potential of mahogany (Swietenia macrophylla King) leaf litter in plant–plant interaction using the sandwich bioassay method has not been reported. The research objectives were to determine and categorize allelopathic potential of S. macrophylla leaf litter using the sandwich bioassay method, and to determine specific activity (EC550). S. macrophylla leaf litter. The results showed that S. macrophylla leaf litter exhibited strong allelopathic activity when compared with 46 leaf litter species and was included in the top ten of allelopathic leaf litter species. Increasing S. macrophylla leaf litter concentration was concomitant with inhibition of radicle lettuce seedling growth compared with the control. According to the linear regression analysis, the effective concentration (EC50) of S. macrophylla was estimated to be 3.25 mg D.W. eq. mL-1 and was considered to have strong growth-inhibitory activity on lettuce radicle elongation. The results suggest the possibility of allelopathic potential of leaf litter in plant–plant interaction under S. macrophylla trees.
Pengenalan Deoxyribonucleic Acid (DNA) Dengan Marker-Based Augmented Reality Siti Nur’aini; Arnia Sari Mukaromah; Siti Muhlisoh
Walisongo Journal of Information Technology Vol 1, No 2 (2019): Walisongo Journal of Information Technology
Publisher : Universitas Islam Negeri Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/wjit.2019.1.2.4531

Abstract

Proses belajar yang baik harus memuat aspek interaktif, memotivasi, menyenangkan dan memberikan ruang bagi siswa untuk dapat mengembangkan kreativitas dan kemandirian. Siswa kadangkala merasa kesulitan pada saat mengillustrasikan isi pembelajaran yang berupa pengetahuan konsep dan prosedur. Dalam pelajaran biologi, materi terkait konsep dasar struktur Deoxyribonucleic Acid (DNA) merupakan materi yang bersifat teoritik dan abstrak. Pemahaman konsep seperti ini  memerlukan penggambaran dan modelling yang lebih realistik agar mudah dipahami. Visualisasi dari sumber belajar dan media belajar yang ada sudah dapat membantu  mempermudah pemahaman  konsep, tetapi variasi media yang lebih nyata, menarik, dan kekinian diharapkan dapat lebih meningkatkan minat siswa.  Pengembangan aplikasi Augmented Reality dapat menjadi salah satu alternatif media pembelajaran DNA. Aplikasi ini dikembangkan dengan metode ADDIE menggunakan Unity3D dan Vuforia. Hasil pengujian fungsional menunjukkan semua fitur dapat berjalan dengan baik sesuai dengan kebutuhan di berbagai versi sistem operasi android. Sedangkan pengujian usability menunjukkan kepuasan mahasiswa sebanyak  86% yang artinya aplikasi ini dapat membantu mahasiswa dalam memahami materi DNA.
Wax Apple (Syzygium samarangense (Blume) Merr. & L.M. Perry): A Comprehensive Review in Phytochemical and Physiological Perspectives Arnia Sari Mukaromah
Al-Hayat: Journal of Biology and Applied Biology Vol 3, No 1 (2020)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v3i1.6070

Abstract

Wax apple (Syzygium samarangense (Blume) Merr. L.M. Perry) is a tropical non-climacteric fruit and belongs to Myrtaceae. It has high diversity in cultivars, metabolite content, and its biological activities. In this comprehensive review, research findings had revealed that various metabolites found in the wax apple have biological activities. The said biological activities such as: antioxidant, immunomodulatory, antibacterial, anticancer, anti-glycation, anti-inflammation, anti-hypertriglyceridemic, anti-hyperglycemic, relaxant, and spasmolytic activities. Other potential use for the metabolites also includes the treatments for neurodegenerative abnormality, analgesic, and central nervous system (CNS) depression agent. Furthermore, wax apple physiological and phytochemical characteristics can be enhanced by girdling technique, hydrogen peroxide treatment, plant growth regulators treatment, and fruit bagging treatment. Meanwhile, postharvest technology of wax apple fruit utilizes nitric oxide treatment, oxalic acid treatment, cold treatment, and Aloe vera gel coating. Wax apple has also been evolved as an ointment, anti-acne cream, and nata de guava. However, environmental stress becomes a challenge in the wax apple plantation. Therefore, the development of molecular biology, chemistry, and postharvest technology will solve those challenges. This review concludes that wax apple (S. samarangense) is a tropical fruit that promises to support the nutritional values and human health.
Pengaruh Kepadatan Medium MS0 terhadap Perkecambahan Biji Jagung (Zea mays L., Var.” Lokal”) secara In Vitro Saniatul Istiqhomah; Arnia Sari Mukaromah; Rusmadi Rusmadi
Al-Hayat: Journal of Biology and Applied Biology Vol 2, No 2 (2019)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v2i2.4664

Abstract

This study aims to determine the effect of MS0 medium density on maize in vitro seed germination. The sample used in this study is corn kernels from local variety (Zea mays L. var. “Lokal”). This research was an experimental research with a quantitative method approach. The study used a Rancangan Acak Lengkap (RAL) with one treatment factor that is medium density MS0 with a degree of agar concentration are 4 gram, 6 gram, 8 gram and 10 gram each concentration repeated 3 times. Data analysis uses the One Way Analysis of Variant (ANOVA) test and if it shows significant results, test continued to BNJ test (Beda Nyata Jujur). The best growth results occur at low level medium density (agar 4 gram) based on parameters days of emerged buds, plant height, number of roots, number of leaves and wet weight of corn plantlets. Uji ANOVA showed that  F value calculated 41.333 bigger than F value table that is 4.07 with significance level 0,05. This result shows that H0 is rejected and Ha is accepted. The results are continued with the BNJ test. BNJ test obtained significantly different results on germination of corn kernels in all treatments. The wet weight of corn plantlets has a high influence on the organs (roots, stems and leaves) of plants. If the wet weight is high, the plant growth is significant and vice versa.
IDENTIFICATION OF POTENTIAL SOIL DEGRADING MICROBIALS CONTAMINATED WITH INSECTICIDES Zhusna Nisha Maulida; Nafrida Noor Azkiya; Lailatuz Zahro; Alfini Siska Dewi Handayani; Tara Puri Ducha Rachmani; Arnia Sari Mukaromah
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol 9, No 1 (2022): August
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v9i1.6364

Abstract

The high use of insecticides can cause soil contamination in the rice field environment, so a solution is needed to reduce the contamination and the negative impact on human health. One of the efforts that can be done to overcome this problem was by bioremediation. The bioremediation technique was chosen due to it is eco- friendly, efficient, and cost-effective in its application. However, bioremediation relies on the capacity of living organisms to absorb, accumulate, translocate and detoxify pollutants in a polluted environment. The objective of this study is to explore microbes that can be used as bioremediation agents in soil exposed to various types of insecticide contamination. The results of this study was as many as ±56 species of microbes can be used as bioremediation agents for various types of insecticides so that bioremediation needs to be carried out in order to avoid pesticide residues on soil and agricultural products.
Potential of biological agents (Pseudomonas sp.) in plastic waste biodegradation process Aisyah Chofifawati; Robbi Mauizzatul Hikmah; Nilana Izzati; Lathifah Nurul Fauzi; Tara Puri Ducha Rahmani; Arnia Sari Mukaromah
Jurnal Biolokus: Jurnal Penelitian Pendidikan Biologi dan Biologi Vol 5, No 2 (2022): December
Publisher : Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/biolokus.v5i2.1192

Abstract

Pseudomonas sp. can degrade plastics because it has an inducible operon system that produces certain enzymes (esterase, serine, hydrolase, and lipase) in the process of carbon source metabolism. The purpose of this study was to determine the potential of Pseudomonas sp. as a plastic biodegradation agent. This research method is a systematic literature review which is carried out by collecting, understanding, analyzing and concluding as many as 30 national and international journal articles published from 2008 to 2022 from several search engines such as Google Scholar, Ncbi, Sinta, and Plos one with the keywords degrading microorganisms plastic. The steps of this research were made samples to be used, tested samples with bacterial isolation, filtered the bacteria, bacterial bacteria and biodegradation within a specified time period by looking at the % loss of plastic before treatment. The results of the literature review analysis showed that Pseudomonas sp. able to degrade plastic around 2-19% by utilizing plastic as a carbon source which will convert addition and condensation polymers into simple molecules such as monomers, dimers and oligomers during the inventory period within 1-3 months. Thus, the microorganisms in the form of Pseudomonas sp. can decompose plastic waste naturally and faster than other methods and does not cause adverse effects.
AUTHENTICATION OF THREE WAX APPLES CULTIVARS (SYZYGIUM SAMARANGENSE (BLUME) MERR. & L.M. PERRY) BASED ON MORPHOLOGICAL CHARACTER AND FRUIT METABOLITE PROFILE Annisa Nur Rachmah; Asri Febriana; Niken Kusumarini; Eka Oktaviani; Arnia Sari Mukaromah
Floribunda Vol. 7 No. 2 (2023): Floribunda April 2023
Publisher : PTTI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32556/floribunda.v7i2.2023.409

Abstract

Wax apple is one of the superior fruits of Demak, especially cultivars 'Citra', 'Delima' and ‘Madu Deli Hijau'. Research on the wax apple of the three cultivars is still limited. Therefore, research on the authentication of wax apple cultivars based on morphological characters and metabolite profiles present in one location is important to do. The aims of this study were to identify morphological characters and analyze metabolite profiles, to analyze relationship and to identify morphological characters and metabolite marker profiles in the authentication activities of three wax apples cultivars. Sampling was in Boyolali Village, Demak Regency. The stages of this research included morphological characterization of stem, leaf, fruit and seed organs, phylogeny analysis with MVSP, metabolite profile analysis using GC-MS, and determination of morphological characters and metabolite profiles with PCA. The results showed that there were morphological diversity and metabolite profile variations of the three cultivars. Relationship analysis based on morphological characters and metabolite profiles resulted in different grouping patterns. 'Madu Deli Hijau' and 'Citra' are closely related based on morphological characters and a combination of morphological characters and metabolite profiles. Morphological characters in the authentication of the 'Citra' (dark red exocarp color), 'Delima' (bright red exocarp color, smooth and flat fruit surface, jagged fruit shape), in 'Madu Deli Hijau' (elongated leaf shape and exocarp is green with a pink tinge). Authentication of probable marker metabolite profiles on 'Citra', 'Delima' and 'Madu Deli Hijau' wax apples of 10, 7 and 5 compounds.
Characteristics of Soto, an ethnic food that reflects Indonesian diversity: Based on ingredients Laras Cempaka; Afifah Amalia Rizki; Nurul Asiah; Wahyudi David; Kurnia Ramadhan; Arnia Sari Mukaromah; Hegar Pramastya; Fadly Husain; Nurul Huda
jurnal1 VOLUME 6 ISSUE 1, JUNE 2023
Publisher : Hasanuddin University Food Science and Technology Study Program

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/canrea.v6i1.680

Abstract

Indonesia is highly diverse in culture, ethnicity, religion, and natural resources. The diversity is also obvious in its cuisine. Soto is an example of an iconic Indonesian dish found in many parts of the country. Despite sharing a similar name, the recipes vary according to their place of origin. Until now, how many variants of Soto are in Indonesia is not exactly known. This review aimed to discuss the characteristics of Soto based on ingredients. Soto is a soup composed of spices-flavored broth and various solid ingredients, such as vegetables and protein-and carbohydrate-rich foods. Every region in Indonesia has a different recipe of Soto; some use non-turmeric soup or coconut milk soup. The carbohydrate ingredients are mainly soun (cellophane noodle) and potato. The protein ingredients are mainly composed of chicken, egg, and beef. Vegetables consist of bean sprouts, tomatoes, and cabbage. Fried garlic, shallot, spring onion, and crackers are added as a garnish. The diversity of the ingredient makes the distinction among Soto: diversity of locality and diversity of ingredients. Approximately 46 types of Soto are reviewed in this article. Most of the Soto recipes use chicken, bean sprouts, soun, boiled egg, garlic, white pepper, red onion, ginger, and bay leaf as the main ingredients. Soto becomes an identity of its geographical origins and has distinction based on the main recipes.
Yeast and Wrapping Materials on The Quality of Tempeh Rachmah, Annisa Nur; Cempaka, Laras; Mukaromah, Arnia Sari
Berkala Ilmiah Biologi Vol 15 No 2 (2024)
Publisher : Fakultas Biologi Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/bib.v15i2.6341

Abstract

Abstract: Tempeh is one of the popular fermented soybeas products in Indonesia and in involves yeast in its manufacture. In addition to its delicious taste and relatively affordable price, tempeh has many nutritional values ​​including vitamin B6 (pyridoxine), vitamin K, protein and high essential amino acids. The good tempeh quality can be affected by materials preference and accuracy throughout making process. Besides soybeans, the tempeh quality is affected by type of yeast and packaging materials. Currently, tempeh making process commonly use instant yeast and plastic as wrapping material than either usar or other wrapping material in the market. The research objective was to elaborate the yeast types impact and wrapping materials on the quality and shelf life of soybean tempeh. The organoleptic assessment was carried out by the hedonic test analyzed by the Kruskal Wallis test and proceeded by the Mann Whitney test if there was a significant difference. Meanwhile, the observation of shelf life was carried out by means of a sensory test until the tempeh rotted. The results showed the type of yeast and packaging materials influencing on the quality of tempeh. Tempeh treatment P5 (using usar and banana leaves) had the highest score in color criteria (3.76±0.0737), smell (2.73±0.1447ceg), texture (2.93±0.1047ceg), taste (3.74±0.1407fj), shelf life (4 days) and faster mold growth than other treatment. According to the results, the panelists preferred tempeh fermented using usar and wrapped in banana leaves, because it was more natural and had the longest durability.
Co-Authors Abdul Malik Afifah Amalia Rizki Aisyah Chofifawati Alfini Siska Dewi Handayani Annisa Nur Rachmah Arfan, Muhammad Ramdhani Asri Febriana Asri Febriana, Asri Chika, Syifara Devi Octavia Eka Oktaviani Fadly Husain Hegar Pramastya Herman Rukmanto Irfan Martiansyah Lailatuz Zahro Laras Cempaka Laras Cempaka Lathifah Nurul Fauzi Mahat Magandhi Meilina, Tiara Dwi Mimin Mimin Muhammad Rifqi Hariri Nafrida Noor Azkiya Niken Kusumarini Niken Kusumarini, Niken Nilana Izzati Nurul Asiah Nurul Huda Rachmah, Annisa Nur Ramadhan, Kurnia Robbi Mauizzatul Hikmah Rusmadi Rusmadi Rusydina, Diyana Sabila S Ma'mun Safitri, Hana Saniatul Istiqhomah Siti Muhlisoh Siti Nur’aini Tara Puri Ducha Rachmani Tara Puri Ducha Rahmani Ulfah, Malia Wahyudi David Yekti Asih Purwestri Yoshiharu Fujii Zhusna Nisha Maulida