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All Journal Jurnal Primatologi Indonesia Jurnal Kedokteran Hewan The International Journal of Tropical Veterinary and Biomedical Research
Ellis Dwi Ayuningsih
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Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Analisis DNA Mikrosatelit untuk Identifikasi Paternitas pada Beruk (Macaca nemestrina) di Penangkaran Pusat Studi Satwa Primata IPB Novita Anggraeni; Ellis Dwi Ayuningsih; Dyah Perwitasari Farajallah; Joko Pamungkas
Jurnal Primatologi Indonesia Vol. 6 No. 2 (2009)
Publisher : Pusat Studi Satwa Primata LPPM-IPB

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (247.743 KB)

Abstract

The increase of population number of pigtailed macaque in Bogor Agricultural University captive breeding facility, was the reason to conduct the research of paternity using microsatellite DNA analysis. Paternity data will be used for determination of Macaca nemestrina colony management. Based on the paternity analysis results showed that in the Koral A, male (No. 6742) is a breeder for offspring (100%), these male is sub-ordinate in that group. Meanwhile, in the Koral B, male (No. 6180) is a breeder for the siblings in that group (92%) this is dominant male, and another male (No. 6747) is 8% of breeder for the existing siblings. On the other hands, the heterozigosity analysis showed that there were ten allele had been detected in locus D1S548, seven alleles in locus D5S820, and six alleles in locus D2S1777 and D3S1768. The amplicon of locus D1SS548, D3S1768, D5S820, D12S1777 are 180-232 bp, 150-178, 189-217, 153-174, respectivelly. The heterozygosis per locus (h) was 0,5168 for D3S1768, D12S1777 and 0,5934 for D1S548. And the average Heterozigosis per all loci       detected was 0.5399.Key words: microsatellite DNA, paternity, beruk, Macaca nemestrina
Bat Coronavirus of Pteropus alecto from Gorontalo Province, Indonesia Wenty Dwi Febriani; Uus Saepuloh; Ellis Dwi Ayuningsih; R. Suryo Saputra; Azhari Purbatrapsila; Meis Jacinta Nangoy; Tiltje Andretha Ransaleh; Indyah Wahyuni; Safriyanto Dako; Rachmitasari Noviana; Diah Iskandriati; Ligaya ITA Tumbelaka; Joko Pamungkas
The International Journal of Tropical Veterinary and Biomedical Research Vol 3, No 2 (2018): Vol. 3 (2) November 2018
Publisher : The Faculty of Veterinary Medicine of Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (249.276 KB) | DOI: 10.21157/ijtvbr.v3i2.12359

Abstract

Bats have been known as natural reservoirs for potential emerging infectious viruses, such as Lyssaviruses, Coronaviruses, Ebola viruses, Nipah virus, and many others. Because of their abudance in population, wide distribution and mobility, bats have a greater risk as source for zoonotic transmission than other animals. Despite the facts of their role as reservoirs for many pathogens, not until an epidemic of Severe Acute Respiratory Coronavirus (SARS-CoV) in 2003 and Middle-East Respiratory Syndrome Coronavirus (MERS-CoV) in 2012, that people pay much attention about coronavirus in bats. SARS-like virus also found in bats with a higher prevalence rate. This study aims to detect the coronavirus of bats in Gorontalo province Indonesia, characterization at the molecular level of the coronavirus genome and determining the level of kinship (through trees filogenetic). This study was conducted as part of bigger PREDICT Indonesia project, in particular to examine coronavirus in bats from Gorontalo province, Indonesia.  As many as  95 rectal swab samples collected from flying foxes (Pteropus alecto) were analyzed in the laboratory using Consensus Polymerase Chain Reaction (PCR) technique to amplify the target sequence from RNA-dependent RNA Polymerase (RdRp) gene with 434 basepair product, resulted 24 samples determined as presumptive positive. Eight out of 24 presumptive positive samples by PCR were analyzed further by nucleotide sequencing and confirmed coronavirus positive. Phylogenetic tree analyses to the eight coronavirus confirmed-sequences were constructed with MEGA-6.0 . The conclusion was 24 out of 95 samples suggested as presumptive positive to Bat CoV. Eight out of 24 samples were analyzed further by nucleotide sequencing and have similarities in the kinship. Three samples had the 98% nucleotide identity to BatCoV from Indonesia and five samples were 85-88% nucleotide identity to BatCoV from Thailand.
IDENTIFICATION AND MOLECULAR CHARACTERIZATION OF BOVINE HERPERVIRUSES (BoHV) DNA TERMINASE PARTIAL GENE IN ACEH CATTLE Prayitno, Lilik; Saepuloh, Uus; Mayasari, Ni Luh Putu Ika; Faisal, Faisal; Ayuningsih, Ellis Dwi; Pamungkas, Joko Pamungkas
Jurnal Kedokteran Hewan Vol 11, No 4 (2017): December
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v11i4.8024

Abstract

Bovine Herpesvirus (BoHV) is a member of Herpesviridae family that acts as pathogenic virus causing infectious bovine rhinotracheitis (IBR) among cattles, resulting in economic loss for cattle industry. BoHV-1 infection in cows is closely related to abortion, respiratory infection, reduced milk production, infertility, and low birth weight. The aim of this study was to identify and characterize the molecular of BoHV-1 and other virus types, as well as the possible presence of other Herpesviridae family using PCR to amplify DNA terminase gene. Four out of 210 nose swab samples were positive for herpes virus on DNA terminase gene. Further characterization of samples showed 99-100% similarity to BoHv-1 and BoHV-6 sequence. Genetic distance between genera BoHV-1 and BoHV-6 is 0.518 and within genera was 0.001 and 0.044. According to phylogenetic tree analysis of DNA terminase gene, the analyzed sequence clustered into 2 genera, namely Varicellovirus which is identical to BoHV-1 and Macavirus which is identical to BoHV-6. The study provides scientific information on molecular characteristics of Herpesviridae family, especially BoHV-1 which is prevalent in Indonesia with the highest density in the central ranches in Aceh province
Co-Authors Azhari Purbatrapsila Diah Iskandriati DYAH PERWITASARI -FARAJALLAH Faisal Faisal Indyah Wahyuni Mayasari, Ni Luh Putu Ika Nangoy, Meis Novita Anggraeni Pamungkas, Joko Pamungkas, Joko Pamungkas Prayitno, Lilik R. Suryo Saputra Rachmitasari Noviana Safriyanto Dako Tiltje Andretha Ransaleh UUS SAEPULOH Uus Saepuloh Wenty Dwi Febriani