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Dilution of Eco Enzyme and Antimicrobial Activity Against Staphylococcus aureus Ginting, Nurzainah; Hasnudi, Hasnudi; Yunilas, Yunilas; Prayitno, Lilik
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 9, No 1 (2022): JITRO, January
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (401.04 KB) | DOI: 10.33772/jitro.v9i1.19705

Abstract

Staphlylococcus aureus is pathogenic bacteria which causes mastitis. The objective of this study is to examine dilution of Eco Enzyme against Staphylococcus aureus. Research design was Completely Randomized Design with three scenarios of diluted Eco Enzyme: T1 = 1: 100 Eco Enzyme dilution; T2 = 1: 200 dilution; and T3 = 1: 300 dilution, using three parameters: antimicrobial zone of inhibition (mm), antimicrobial index (mm), and pH.  Eco Enzyme was produced from the fermentation of fruits containing enzymes, organic acids and microbial. The Eco Enzyme is applied, among others, as biodesinfectant in animal sheds. The results showed that Eco Enzyme either with 1:100, 1:200, or 1:300 concentrations has been proven to be able to inhibit Staphylococcus aureus. Meanwhile, the 1:100 dilution showed a strong inhibition.Keywords: animal sheds, bacteria, biodesinfectant, eco enzyme, mastitis
IDENTIFICATION AND MOLECULAR CHARACTERIZATION OF BOVINE HERPERVIRUSES (BoHV) DNA TERMINASE PARTIAL GENE IN ACEH CATTLE Prayitno, Lilik; Saepuloh, Uus; Mayasari, Ni Luh Putu Ika; Faisal, Faisal; Ayuningsih, Ellis Dwi; Pamungkas, Joko Pamungkas
Jurnal Kedokteran Hewan Vol 11, No 4 (2017): December
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v11i4.8024

Abstract

Bovine Herpesvirus (BoHV) is a member of Herpesviridae family that acts as pathogenic virus causing infectious bovine rhinotracheitis (IBR) among cattles, resulting in economic loss for cattle industry. BoHV-1 infection in cows is closely related to abortion, respiratory infection, reduced milk production, infertility, and low birth weight. The aim of this study was to identify and characterize the molecular of BoHV-1 and other virus types, as well as the possible presence of other Herpesviridae family using PCR to amplify DNA terminase gene. Four out of 210 nose swab samples were positive for herpes virus on DNA terminase gene. Further characterization of samples showed 99-100% similarity to BoHv-1 and BoHV-6 sequence. Genetic distance between genera BoHV-1 and BoHV-6 is 0.518 and within genera was 0.001 and 0.044. According to phylogenetic tree analysis of DNA terminase gene, the analyzed sequence clustered into 2 genera, namely Varicellovirus which is identical to BoHV-1 and Macavirus which is identical to BoHV-6. The study provides scientific information on molecular characteristics of Herpesviridae family, especially BoHV-1 which is prevalent in Indonesia with the highest density in the central ranches in Aceh province
Enhancing Farmers’ Literacy on Foot-and-Mouth Disease through a Mobile Expert System Application in Labuhanbatu Utara: Peningkatan Literasi Peternak akan PMK (Penyakit Mulut dan Kuku) melalui Aplikasi Mobile Sistem Pakar di Labuhanbatu Utara Hasibuan, Lailan Sahrina; Prayitno, Lilik; Giri, Endang Purnama; Kustiyo, Aziz; Adrianto, Hari Agung
Dinamisia : Jurnal Pengabdian Kepada Masyarakat Vol. 9 No. 2 (2025): Dinamisia: Jurnal Pengabdian Kepada Masyarakat
Publisher : Universitas Lancang Kuning

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31849/dinamisia.v9i2.23983

Abstract

Indonesia, causing distress among farmers due to their limited knowledge of how to handle the disease. This situation has been exploited by unscrupulous traders and middlemen who spread misinformation, claiming that cattle infected with FMD will inevitably die. Taking advantage of the panic, they purchase the animals at low prices and resell them at higher prices after treatment. To address this issue, a team of researchers from IPB University carried out a community outreach program aimed at improving farmers' literacy regarding FMD. As part of the program, a mobile-based expert system application was introduced, enabling farmers to perform quick, independent diagnoses based on observed symptoms. The evaluation results, based on pre-test and post-test assessments, showed a significant increase in farmers' understanding after attending the seminar. This innovation is expected to empower farmers to manage FMD more independently and effectively.