Romiyadi Romiyadi
Program Studi Agroteknologi Fakultas Pertanian Universitas Winaya Mukti

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Keragaan tiga jenis planlet anggrek Phalaenopsis asal Protocorm yang diinduksi Ethyl Methyl Sulfonate (EMS) secara in vitro Romiyadi Romiyadi; Ai Komariah; Suseno Amien
Kultivasi Vol 17, No 1 (2018)
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1018.873 KB) | DOI: 10.24198/kultivasi.v17i1.16077

Abstract

Abstract. This research was conducted to determine the effect of concentration of Ethyl Methyl Sulfonate (EMS) to performance of three types of Phalaenopsis Planlets and to know the optimum concentration of EMS on any type of Phalaenopsis orchids. The experiment was conduc-ted at Tissue Culture Laboratory of the Faculty of Agriculture, Winaya Mukti University, Sumedang. It used a completely randomized design (CRD) with factorial pattern, that consisted of two factors and replicated twice.The first factor was the type of Phalaenopsis that resulted form hybridization which consisted of v1 (Phalaenopsis 717 X Phalae-nopsis Fire Bird), v2 (Phalaenopsis Tianong Rose X Sibling), and v3 (Phalaenopsis Luchia Pink X Phalae-nopsis Chain Xen Mammon). The second factor was the concentration of EMS that consisted of e0 (0% EMS/control), e1 (0,05% EMS), e2 (0,10% EMS), e3 (0,15% EMS), e4 (0,20% EMS), and e5 (0,25% EMS). Explant protocorm of three types of Phalae-nopsis soaked in a solution of EMS by each treat-ment for 3 hours, and cultured on MS medium Basal Modified Multiplication Shoot for 10 weeks. The experimental results showed that there are interaction between the three types of Phalae-nopsis result of a cross with a concentration of EMS to variable number of roots. Orchids P. 717 X P. Fire Bird had higher  number of leaves, number of roots, leaf leang, and root length than the other. The result showed that there were interaction between three species of Phalaenopsis orchid from the crossing with EMS concentration on root variables. Orchid P. 717 X P. Fire Bird has the number of leaves, the number of roots, the number of shoots, leaf length and root length better than other types. EMS concentrations independently at all levels of treatment can not increase the number of leaves, the number of roots, fresh weight of planlet, leaf length, and root length.Keywords: Phalaenopsis, the concentration of EMS, in vitro cultureSari. Penelitian ini mempelajari dan mengetahui pengaruh konsentrasi Ethyl Methyl Sulfonate (EMS) terhadap keragaan planlet tiga jenis anggrek Phalaenopsis asal protocorm dan mencari konsentrasi optimum EMS untuk setiap jenis anggrek Phalae-nopsis secara in vitro.Percobaan dilaksanakan di Laboratorium Kultur Jaringan Fakultas Pertanian Universitas Winaya Mukti Sumedang. Eksperimen menggunakan Rancangan Acak Lengkap (RAL) Pola Faktorial, yang terdiri atas dua faktor dan diulang sebanyak dua kali.Faktor pertama adalah jenis anggrek Phalaenopsis hasil persilangan yang terdiri atas tiga jenis, yaitu v1 (Phalaenopsis 717 X Phalaenopsis Fire Bird), v2 (Phalaenopsis Tianong Rose X Sibling), dan v3 (Phalaenopsis Luchia Pink X Phalaenopsis Chain Xen Mammon). Faktor kedua adalah EMS yang terdiri atas enam taraf perlakuan, yaitu e0 (0% EMS/kontrol), e1 (0,05% EMS), e2 (0,10% EMS), e3 (0,15% EMS), e4 (0,20% EMS), dan e5 (0,25% EMS). Eksplan berupa protocorm dari tiga jenis anggrek Phalaenopsis hasil persilangan yang direndam dalam larutan EMS berdasarkan masing-masing perlakuan selama 3 jam, dan dikulturkan pada media MS Modified Multiplication Shoot Basal selama 10 minggu.Hasil penelitian menunjukkan terjadi interaksi antara tiga jenis anggrek Phalaenopsis hasil persilangan dengan konsentrasi EMS terhadap variabel jumlah akar.Anggrek P. 717 X P.Fire Bird memiliki jumlah daun, jumlah akar, jumlah tunas, panjang daun dan panjang akar yang lebih baik dibandingkan jenis lainnya. Konsentrasi EMS secara mandiri pada semua taraf perlakuan tidak dapat meningkatkan jumlah daun, jumlah akar, bobot segar planlet, panjang daun, dan panjang akar.Kata Kunci: Phalaenopsis, Konsentrasi EMS, dan Budidaya In Vitro
Pengaruh Konsentrasi Air Rendaman Sabut Kelapa dan Dosis Pupuk NPK Terhadap Pertumbuhan dan Hasil Tanaman Melon Varietas Action 434 Romiyadi Romiyadi; Endang Sufiadi
Paspalum: Jurnal Ilmiah Pertanian Vol 3, No 1 (2015)
Publisher : Lembaga Penelitian dan Pengabdian Universitas Winaya Mukti

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (581.389 KB) | DOI: 10.35138/paspalum.v3i1.12

Abstract

The experiment was conducted at Faculty of Agriculture UNWIM Tanjungsari - Sumedang, at the altitude of 850 m above sea level (asl) with ground Andisol orders from February to April 2008. The purpose of an experiment to study the effect of the interaction between the concentration of coco water immersion ata dose NPK fertilizer on the growth and yield of melon varieties Action 434. The experimental design used was a randomized block design factorial design consisting of two factors and repeated twice. The first factor is the concentration of water immersion coco (k), which consists of five levels, namely: k0 = 0%, 25% = k1, k2 = 50%, k3 = 75%, and k4 = 100%. The second factor is the dose of NPK fertilizer (d),which consists of four levels, namely: d0 = 0 g plant-1, d1 = 10 g plant-1, d2 = 20 g plant, and d3 = 30 g plant-1. The results showed that there is an interaction effect between the concentration of coco water immersion at a dose of NPK fertilizer to the plants age 20 DAT (days after transplanting) and 30 DAT, as well as the number of leaves at the age of 30 DAT. Coco water immersion at a concentration of 25% gives a better effect on the variable length of the plant, number of leaves, fruit weight per plant, and the quality of the fruit skin. Coco water immersion at a concentration of 50% gives better effect on thevariable sweetness of the fruit flesh. Without dosing NPK fertilizer already gives a better effect on the variable length of the plant, number of leaves, fruit weight per plant, diameter of fruit per plant, and the thickness of the flesh of the fruit. NPK fertilizer dose of 10 g plant-1 give better effect to the variable length of the plant, number of leaves, fruit weight per plant, diameter of fruit per plant, the thickness of the flesh of the fruit, the sweetness of the fruit, and the quality of the fruit skin.
Pengaruh Konsentrasi Zat Pengatur Tumbuh Naphthalene Acetic Acid dan Benzil Amino Purin terhadap Pertumbuhan Protokorm Anggrek Dendrobium spectabile pada Kultur In Vitro Ujang Siron; Noertjahyani Noertjahyani; Yana Taryana; Romiyadi Romiyadi
Paspalum: Jurnal Ilmiah Pertanian Vol 7, No 1 (2019)
Publisher : Lembaga Penelitian dan Pengabdian Universitas Winaya Mukti

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (616.373 KB) | DOI: 10.35138/paspalum.v7i1.107

Abstract

The aim of the article is to study the interaction effect between NAA and BAP concentration on protocorm growth and to know the proper concentration for growth of Dendrobium  spectabile  orchid protocorm. This research method using an experimental method which is conducted in the Laboratory of Agriculture Faculty of Winaya Mukti University, Tanjungsari Subdistrict, Sumedang District. The experiment was conducted from June 2017 until September 2017. The experiment used was a Completely Randomized Design (CRD) with the factorial pattern, consisting of two factors and repeated twice. the first factor was the effect of NAA concentration which consisted of five levels, namely without NAA, 0.5 mg kg-1, 1.0 mg kg-1, 1.5 mg kg-1, and 2.0 mg kg-1.  The second factor is the BAP concentration which consists of five levels, namel without BAP, 1.0 mg kg-1, 2.0 mg kg-1, 3.0 mg kg-1, and 4.0 mg kg-1. Eksplant is protocorm from orchid D. spectabile which is grown on MS medium (Murashig and Skoog) half recipe as base medium accompanied by each treatment for 12 weeks. The experimental results show that there is an interaction between the effect of NAA and BAP concentration on the number of leaves only. Without NAA or 1.5 mg kg-1 NAA concentration with BAP 2.0 mg kg-1 gives more leaves.  Independent of NAA or BAP concentrations did not affect the number of buds, number of roots, root length, fresh and dry weight of plantlets, and also growth ability of plantlets.  BAP concentration only affect plant height, and the highest plantlet height is found without add of BAP in medium culture 
Respon Pertumbuhan Tanaman Anggrek (Phalaenopsis fimbriata JJ. Smith) Akibat Perlakuan Berbagai Media Tanam Di Pembenihan Hilda Nur Syafira; Ai Komariah; Reni Nurhayatini; Romiyadi Romiyadi
OrchidAgro Vol 2, No 1 (2022)
Publisher : Fakultas Pertanian Universitas Winaya Mukti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35138/orchidagro.v2i1.368

Abstract

Penelitian ini bertujuan untuk mendapatkan media tanam yang mampu memberikan pertumbuhan terbaik terhadap anggrek Phalaenopsis fimbriata. Penelitian ini dilaksanakan di Screen house, Puskopad, Kecamatan Tanjungsari, Kabupaten Sumedang dengan ketinggian tempat 850 meter di atas permukaan laut (Mdpl). Rancangan yang digunakan dalam penelitian ini adalah Rancangan Acak Kelompok (RAK) sederhana dengan 4 ulangan dan 7 taraf, yaitu A = Sphagnum moss, B = Pakis, C = Akar Kadaka, D = Cocofiber, E = Sekam Bakar, F = Cocochip dan G = Hidrogel. Hasil penelitian menunjukan: 1. Berbagai media tanam dapat berpengaruh terhadap pertumbuhan benih anggrek Phalaenopsis fimbriata pada variabel pertumbuhan jumlah daun, panjang daun, lebar daun dan bobot segar.2. Media tanam akar kadaka memberikan pertumbuhan terbaik pada benih anggrek Phalaenopsis fimbriata pada variabel pertumbuhan jumlah daun, panjang daun, lebar daun dan bobot segar.
Uji Pertumbuhan dan Hasil Berbagai Varietas Tanaman Stroberi (Fragaria sp.) di Dataran Rendah Romiyadi Romiyadi; Nur’aini Islami Putri
Jurnal Greenation Pertanian dan Perkebunan Vol. 2 No. 2 (2024): (JGPP) Jurnal Greenation Pertanian dan Perkebunan (Mei 2024)
Publisher : Greenation Publisher & Yayasan Global Research National

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.38035/jgpp.v2i2.180

Abstract

Produksi stroberi di Indonesia sedang mengalami peningkatan namun, kendala dalam usaha petani belum mampu memenuhi permintaan pasar karena keterbatasan kemampuan petani. Dalam upaya lebih ditingkatkan kembali produksi stroberi dibudidayakan di berbagai daerah dapat dilakukan dengan alternatif yaitu penggunaan varietas unggul yang dapat tumbuh di dataran rendah dengan harapan produksi dapat optimal baik secara kualitas maupun kuantitas. tujuan penelitian ini untuk mempelajari pertumbuhan dan hasil berbagai varietas tanaman stroberi di dataran rendah. Penelitian ini menggunakan percobaan lapangan di kebun percobaan, Desa Karangsong, Kecamatan Indramayu, Kabupaten Indramayu dengan ketinggian 0,5 mdpl dan dilaksanakan pada bulan April-Juni 2024, Penelitian ini menggunakan Rancangan Acak Kelompok (RAK) Sederhana yang terdiri dari 1 faktor yaitu varietas stroberi. Masing-masing diulang sebanyak 5 kali, sehingga didapat 25 kombinasi perlakuan. A : Varietas Manora, B : Varietas Mencir, C : Varietas Red Giant, D : Varietas Sagahonoka dan E : Varietas Merlan. Hasil penelitian menunjukkan bahwa Pertumbuhan dan hasil berbagai varietas tanaman stroberi di dataran rendah yaitu perlakuan B (Mencir) dapat berpengaruh baik terhadap parameter tinggi tanaman, jumlah daun, jumlah buah, tingkat kemanisan buah dan bobot buah per tanaman.
Pengaruh Konsentrasi AB Mix Terhadap Pertumbuhan dan Hasil Microgreens Tanaman Okra (Abelmoschus esculentus (L.) Moench.) Romiyadi; Hariandi Napitupulu, Ricki; Budiasih; Amalia, Lia
Jurnal Greenation Pertanian dan Perkebunan Vol. 3 No. 1 (2025): (JGPP) Jurnal Greenation Pertanian dan Perkebunan (Februari 2025)
Publisher : Greenation Publisher & Yayasan Global Research National

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.38035/jgpp.v3i1.255

Abstract

Penelitian ini bertujuan untuk mempelajari pengaruh konsentrasi AB Mix terhadap pertumbuhan dan hasil microgreens tanaman okra dan mendapatkan rekomendasi konsentrasi AB Mix terbaik. Penelitian ini dilaksanakan di Green House Fakultas Pertanian Universitas Winaya Mukti-Sumedang, Jawa Barat dengan ketinggian tempat 870 m di atas permukaan laut. Waktu pelaksanaan pada bulan Juli sampai dengan bulan Agustus 2024. Penelitian menggunakan Rancangan Acak Kelompok Tunggal dengan 5 taraf perlakuan (A: 0 mg L-1 larutan AB Mix , B: 150 mg L-1 larutan AB Mix, C: 300 mg L-1 larutan AB Mix, D: 450 mg L-1 larutan AB Mix dan E: 600 mg L-1 larutan AB Mix) diulang sebanyak 5 ulangan. Hasil penelitian menunjukan bahwa konsentrasi AB Mix berpengaruh terhadap pertumbuhan dan hasil microgreens tanaman okra, yaitu pada variabel tinggi tanaman 11 HSS dan 14 HSS; jumlah tanaman hidup; bobot segar microgreens per thinwall dan kadar vitamin C. Konsentrasi 150 mg L-1 larutan AB Mix memberikan pengaruh lebih baik terhadap pertumbuhan dan hasil microgreens, tetapi konsentrasi 0 mg L-1 larutan AB Mix memberikan pengaruh terhadap kadar vitamin C microgreens paling tinggi.
Effect Of The Combination of Concentration of Naphtalene Acetic Acid (NAA) and Kinetin On The Growth of Fig Plants (Ficus carica L.) of the Green Jordan Variety in Vitro Rusmana Putri, Devina Aulya; Komariah, Ai; Romiyadi, Romiyadi
Jurnal Agrosci Vol 3 No 2 (2025): Vol 3 No 2 November 2025
Publisher : Ann Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62885/agrosci.v3i2.971

Abstract

Background. The fig plant is one of the plantation commodities that experiences a decline in production every year. The cause of the decline in fig production is the low multiplication of fig plants due to less-than-optimal plant cultivation. Fig plants have high economic value, where market demand is increasing, but production of fig plants is decreasing. Therefore, efforts are needed to increase productivity, one of which is the use of in vitro propagation techniques with the addition of NAA and Kinetin growth regulators. Aims. This research aims to investigate the growth response to the combination of Naphthalene Acetic Acid (NAA) and Kinetin concentrations on the growth of fig (Ficus carica L.), specifically the Green Jordan variety, in vitro. Methods. This experiment was carried out at the Tissue Culture Laboratory of the Regional Technical Implementation Unit, Horticulture Seed Center, Hegarmanah, Jatinangor District, Sumedang Regency, West Java. Conducted from March to May 2024, the experiment employed a Simple Randomized Environmental Design (SRE) consisting of 5 treatment combinations, repeated 4 times, resulting in 20 experimental units. A = (0 mg L-1 NAA + 4 mg L-1 Kinetin ), B = (0.5 mg L-1 NAA +3 mg L-1 Kinetin ), C = 1 mg L-1 NAA +2 mg L-1 Kinetin, D = (1.5 mg L-1 NAA + 1 mg L-1 Kinetin ), E = (2 mg L-1 NAA + 0 mg L-1 Kinetin ), Observational data were analyzed using the F test and if there is an effect followed by Duncan's Multiple Range Test at the 5% level. Conclusion. The results showed that the combination concentration (4 mg L-1 Kinetin) had a better effect on the percentage of leaves.