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All Journal HAYATI Journal of Biosciences Jurnal Veteriner Jurnal Biologi Udayana Proceedings of Annual International Conference Syiah Kuala University - Life Sciences & Engineering Chapter
NASTITI KUSUMORINI
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Growth and Development of the Uterus and Placenta of Superovulated Gilts REVOLSON ALEXIUS MEGE; SYAHRUN HAMDANI NASUTION; NASTITI KUSUMORINI; WASMEN MANALU
HAYATI Journal of Biosciences Vol. 14 No. 1 (2007): March 2007
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (38.729 KB) | DOI: 10.4308/hjb.14.1.1

Abstract

Forty eight gilts with average body weight of 107.83 + 5.08 kg were used in experiments to study the use of pregnant mare serum gonadotrophin (PMSG) and human chorionic gonadotrophin (hCG) as superovulation agent in gilts to increase piglet production. Four groups of twelve gilts were injected with PMSG dan hCG dose levels of 0, 600, 1200, and 1800 IU/gilt. Injections were conducted three days before estrus. During gestation, gilts were placed in colony pigpens. On days 15, 35, and 70 of gestation, gilts were slaughtered in order to measure the number of corpus luteum, growth and development of the uterus and placenta. Blood samples were collected to determine progesterone and estradiol concentrations. The resuts showed that superovulation dose levels of 600 to 1200 IU/gilt increased progesterone and estradiol secretions, growth and development of the uterus and placenta in gestation ages of 15, 35, and 70 days. It is concluded that superovulation with dose of 600 to 1200 IU can improve the gilts reproduction. Key words: gilts, superovulation, gestation, progesterone, estradiol, corpus luteum
Supplementation of tempeh extract for improving quality of bone in premenopausal conditions using rats as animal models . Safrida; Nastiti Kusumorini; Wasmen Manalu; Hera Maheshwari
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 3, No 1 (2013): Life Sciences
Publisher : Syiah Kuala University

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Abstract

Tempeh extract is a natural substance that contains phytoestrogens, having similar estrogen activity. This study was designed to determine the potential of tempeh extract in improving the quality bone in premenopausal conditions, and to compare the natural product of tempeh extract with commerciallyavailable hormones (genistein, ethinylestradiol, and somatotropin). Experimental design used was Completely Randomized Design (CRD) consisted of 7 experimental groups, each consisted of 3 rats i.e.,1) K= premenopausal rats as a negative control, 2) P = premenopausal rats given distilled water orally as a placebo, 3) TEM = premenopausal rats given tempeh extract 300 mg/day/200g body weight, 4) GEN =premenopausal rats given genistein 0.25 mg/day/kg body weight, 5) EST = premenopausal rats givenethinylestradiol 9x10-3 mg/day/200g body weight, 6) SO = premenopausal rats injected with sesame oil/day/kg body weight, 7) BST = premenopausal rats injected somatotropin 9 mg/day/kg body weight. The parameters observed were the bone and serum calcium and phosphorus concentrations, bone ashconcentrations, bone collagen concentrations, bone water concentrations, bone RNA concentrations, bone length, bone weight, bone density, and bone strength. Supplementations of tempeh extract for two months in premenopausal rats could improve the quality of bone, which was characterized by the increased bone calcium concentrations, ratio of Ca/P on tibia bone, bone ash levels, bone density, and bone strength
Profil Leukosit, Diferensial Leukosit, dan Indeks Stres Luwak Jawa (Paradoxurus hermaphroditus) (LEUCOCYTE COUNT, LEUCOCYTE DIFFERENTIATION, AND STRESS INDEX OF COMMON PALM CIVETS (PARADOXURUS HERMAPHRODITUS) Aryani Sismin Satyaningtijas; Nastiti Kusumorini; Moh. Mursyid Fachrudin; Purnomo .
Jurnal Veteriner Vol 15 No 4 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Common palm civets (Paradoxurushermaphroditus) have a potential to be commercial commodityanimal which produce best coffee, parfume, but also as reservoir potential infection desease such as SevereAcute Respiratory Syndrome (SARS). Therefore, their health status is very important. This study wasaimed to discribe the leukocyte profile, and stress index of 4 males Java common pal civets and 4 females.Blood sampling was taken from vena femoralis and done at weeks 1st, 5th, 6th, and 7th. Leukocyte profilewere examined using haemocytometerand blood smear. The average leukocyte count of males and femalescommon palm civets per mm3 were (3.33±0.86)x103 and (2.83±0.70)x103, neutrophil were (1.01±0.47)x103and (0.68±0.30)x103, eosinophil were (0.16±0.18)x103 and (0.04±0.05)x103, lymphocyte were (2.06±0.42)x103and (2.05±0.59)x103, monocyte were (0.09±0.06)x103 and (0.06±0.06)x103. Stress index were (0.49±0.18)for males and (0.37±0.22) for females. Generally, leukocyte count of males Java common palm civet werehigher than those of females.
Aktivitas Antioksidan Ekstrak Daun Cengkeh (ANTIOKSIDANT ACTIVITY OF CLOVE LEAF EXTRACT) Andi Mu’nisa; Tutik Wresdiyati; Nastiti Kusumorini; Wasmen Manalu
Jurnal Veteriner Vol 13 No 3 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Antioxidant activity of clove leaf was investigated. The clove leaves (Eugenia aromatica) wereprepared with reflux extraction using methanol, water, and ethanol. Total activities of the extractsand reducing power were measured with thiocyanate method and reducing potential method. Resultshowed that the highest total antioxidant activity was observed in methanol extract. It appearsthat the ability of this extract for partitioning at the interface of emulsion in tested oxidationsystem was the highest among the other extracts, therefore it had the best activity to inhibitoxidation. The type of phenolic compounds of this extract appeared to be responsible for the highestradical scavenging capacity. The same phenomenon occurred for reducing power, methanol extracthad the highest reducing power, thereby suggesting that each extracts comprised different type ofphenol based on different polarity of reflux used for extraction. Total antioxidant activity and thehighest reducing power obtained from methanol extract. Both are closely related to total phenolcontent of the clove leaves.
Perbaikan Respons Seluler pada Penuaan Hipokampus yang Diperantarai Glutation Hasil Pemberian Alanin-glutamin Dipeptida (IMPROVEMENTS CELLULAR RESPONS IN AGED HIPPOCAMPUS RELATED GLUTATHIONE RESULT OF THE ADMINISTRATION OF ALANINE-GLUTAMINE DIPEPTIDE) Sunarno .; Wasmen Manalu; Nastiti Kusumorini; Dewi Ratih Agungpriyono
Jurnal Veteriner Vol 14 No 1 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Physiological aging or aging due to oxidative stress decrease glutathione level in the hippocampuswhich impacts the respons impaired hippocampus celuller. Hippocampus cellular respons disorderscharacterized with decreased viability, increased mortality, and the shortening of the axons of neurons.One way to improve hippocampus cellular respons is to  increase the levels of glutathione and theconcentration of glutathione precursor. One compound that provides glutathione precursors is alanine-glutamine dipeptide. This research was designed to obtain the improve of hippocampus cellular responsresult from the administration of 7% alanine-glutamine dipeptide concentration of aged or oxidative-stressed rats. The improvement of hippocampus cellular respons affect  the improvement of the hippocampus function. The experimental rats were assigned into a completely randomized design consisted of threefactors with 2x2x2 factorial arrangement. The first factor was the age of the experimental rats, consistedof two levels i.e., 12 and 24 months. The second factor was oxidative stress consisted of two levels, i.e.,without and with oxidative stress. The third factor was alanine-glutamine dipeptide administrationconsisted of 2 concentrations, i.e. 0% and 7%. The results showed that  administration of 7% alanine-glutamine dipeptide improved level of glutathione in the hippocampus either in younger (58,76%) or aged(125,81%) rats or in normal (76,47%) and in oxidative-stressed rats (97,26%). These antioxidant hadmediated the respons improve viability, mortality, and long axons responses of neurons at younger (4,11%,37,07%, and 12,58%) or aged (6,91%, 37,85%, and 32,84%) rats, in normal (3,25%, 29,21%, and 21,04%)and oxidative stress (7,80%, 43,01%, dan 25,56%) rats. This research concluded that the alanine-glutaminedipeptide 7% increased glutathione levels.  This increased level affected the improvement of cellularresponds in aging hippocampus, physiological aging, or aging due to oxidative stress in rats.
Pemberian Vaksin Ichthyophthirius multifiliis untuk Mencegah Ichthyophthiriasis pada Ikan Mas (APPLICATION OF ICHTHYOPHTHIRIUS MULTIFILIIS VACCINE FOR PREVENTION OF ICHTHYOPHTHIRIASIS IN COMMON CARP) Henni Syawal; Nastiti Kusumorini; Wasmen Manalu; Ridwan Affandi
Jurnal Veteriner Vol 17 No 1 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This study was conductedto assess the physiological responses of commoncarp due to administrationof ich vaccines and kept at different water temperatures. Completely Randomized Factorial Designwasusedin this study. The number and the size of the experimental fish was720 and 5-7 cm, respectively. Fish wasvaccinated by immersing the fish in water that contained 3 mLL-1 of ich vaccines for 15 minutes. Then, fishwas kept for 21 days at various temperatures: at 18ºC which was room temperature without heater andfish had not been vaccinated and at 20, 24, and 28ºC where fish was given the vaccines and the aquariumis facilitated with electric heater. Atday15post immunization, challenge test with 5.000 cells of life therontfish-1was performed.Immobilization test, differential leukocyte, prevalence rates, and survival rates weremeasured. The measurements of these parameters were performed four times:before the treatment (day0), day7, day14, and day21, respectively. The results showed that the vaccine could reduce the stress inthefish that were kept at 20, 24, and 28ºC, and the prevalence of ichthyophthiriasis. In addition the vaccineincrease the fish survival rates after challenge test. The highest survival rate (100%) was found in fish thatwerekept in 28ºC.
PERTUMBUHAN DAN AKTIVITAS ANAK TIKUS PADA PEMAPARAN CAHAYA YANG BERBEDA Ida Bagus Made Suaskara; Nastiti Kusumorini; Nurhidayat -
Jurnal Biologi Udayana Vol XI, No 1
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

Empat puluh lima (45) ekor anak tikus jantan umur 1 hari digunakan pada penelitian ini. Anak tikus dibagi menjadi satu kelompok kontrol dan dua kelompok perlakuan yaitu; 1) Kelompok perlakuan gelap (D) dan kelompok ini dibagi lagi menjadi 4 sub kelompok perlakuan berdasarkan jumlah hari gelap yaitu; a) 6 hari (D6), b) 12 hari (D12), c) 18 hari (D18) dan d) 24 hari (D24), dan 2) kelompok perlakuan cahaya (L) juga dibagi menjadi 4 sub kelompok yaitu; a) 6 hari (L6), b) 12 hari (L12), c) 18 hari (L18) dan d) 24 hari (L24). Hasil penelitian ini menunjukkan bahwa lama pemberian cahaya memberikan pengaruh yang nyata terhadap pertumbuhan dan perkembangan Nukleus Suprachiasmatik (NSC). Pertumbuhan anak tikus pada pemaparan cahaya yang berbeda selama periode menyusui menyebabkan rataan pertambahan berat badan baik pada kelompok perlakuan gelap maupun cahaya sangat lambat dengan meningkatnya lama perlakuan. Aktivitas anak tikus berupa (jarak tempuh, awal pergerakan, gerakan di tempat maupun total pergerakan di tempat, dan lama waktu istirahat semakin pendek), cenderung meningkat sebanding dengan lama cahaya. Sedangkan pada perlakuan gelap terjadi sebaliknya yaitu cenderung menurun. Tidak ada perbedaan pengaruh pemaparan cahaya dan gelap terhadap perubahan kadar hormon T3 dalam plasma darah tikus.
Co-Authors . Safrida A.S. Satyaningtijas Andi Mu’nisa Dewi Ratih Agungpriyono Henni Syawal HERA MAHESHWARI Ida Bagus Made Suaskara Moh. Mursyid Fachrudin Nurhidayat - Purnomo . Revolson Mege RIDWAN AFFANDI Sunarno . SYAHRUN HAMDANI NASUTION Tutik Wresdiyati Wasmen Manalu