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All Journal Jurnal Ilmu Pertanian Indonesia Hemera Zoa Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati
Dewi Ratih Agungpriyono
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Deteksi Dini Penyakit Tumor Sel Darah Myelosit Leukosis melalui Pemeriksaan Ulas Darah Dewi Ratih Agungpriyono; Hernomoadi Huminto; Sri Estuningsih; Aryani Sismin Satyaningtijas
Jurnal Ilmu Pertanian Indonesia Vol. 12 No. 2 (2007): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

Myelocytes blood cell tumor in chicken is a disease caused by retrovirus, avian leukosis virus subgroup J (ALV-J). The virus has the same group as human retrovirus (HIV) which caused AIDS, but the avian type possesses oncogenic properties, that could induce cell transformation and tumor formation. ALV-J stimulates the bone marrow's myelocytes and transforms them into tumor cell myelocytoma. The tumor cells then metastasis through the circulatory system causing myeloid leukosis and tumor cells accumulation in various internal organs or myelocytomatosis. This study was done on the base of the leukosis behavior of the tumor. The finding of the metastasis tumor cell in the blood smear examination is thought could be use as the diagnostic clue of the disease. Blood smear from sick chickens are collected and stained with some chemical staining substance such as may grunwald-giemsa, hematoxyllin eosin, periodic acid Schiff, congo red, toluidine blue, and sudan black B. Cytochemistry character of the blood cells was observed using light microscope. The result showed that myelocytes granules were best observed using hematoxyllin eosin, periodic acid Schiff, congo red and toluidine blue while may grunwald-giemsa, and sudan black B could not differentiate the granules. By this method, the field veterinarian will able to screen the suspected chicken flock for myeloid leukosis earlier than the occurrence of tumor formation.
Komunitas Makrozoobentos dan Akumulasi Kromium di Sungai Cimanuk Lama, Jawa Barat Arbi Mei Gitarama; Majariana Krisanti; Dewi Ratih Agungpriyono
Jurnal Ilmu Pertanian Indonesia Vol. 21 No. 1 (2016): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (601.419 KB) | DOI: 10.18343/jipi.21.1.48

Abstract

The residue of human activities and batik industrial waste water surrounding the river will be able to increase the accumulation of chromium and to disrupt macrozoobenthic communities in the river. The aims of this study was to assess the impact of human activities surrounding the river to the macrozoobenthic communities and the accumulation of chromium in Cimanuk Lama River, Indramayu District, West Java. The study has been conducted from AprilMay 2015 based on three times sampling at three different sampling stations. The result of this study shows that the chromium accumulated in the waters of all station still meet the standard quality ranging from 0,010-0,016 mg/l, but only station 1 fulfills the standard quality for chromium accumulated in the river sediment with the range of all stations was about 11,72-46,63 mg/kg. The results also show that the community index analysis describes the change of macrozoobenthic community at all stations caused by environmental pressure, especially at the station 2 which is indicated by the highest score of Family Biotic Index. The accumulation of the chromium in the waters and the change of macrozoobenthic community structure are mostly influenced by the use of the Cimanuk Lama river long for agricultural and fisheries activities, and also batik home industry.
PAT-6 Brain Histopathology of Cynomolgus Monkey (Macacafascicularis) with Memory Impairment Indicated by Alzheimer Type Neurodegenerative Disease Vinka Aftinata Kusumaputri; Huda Shalahudin Darusman; Dewi Ratih Agungpriyono
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

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The study is about the degenerative lesion found in nine cynomolgus monkeys that showed decline cognitive function during theirold age life. In previous studies, the brain of these cynomolgus monkeys has been tested positive for amyloid deposits by using the ELISA test.
Pengaruh Pemberian Ekstrak Kulit Batang Ailanthus altissima terhadap Perubahan Sel Hati dan Ginjal Mencit Praptiwi Praptiwi; Emma Sri Kuncari; Dewi Ratih Agungpriyono; Chairunnisa Chairunnisa
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 1 (2010): February 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v15i1.2654

Abstract

The aim of this study was to determine the toxicopathological effect of Ailanthus altissima extract in liver and kidney of the mouse. The treatments in this study were: (1) negative control (carboxy methocellulose), extracts with concentration of (2) 1 mg/kg BW, (3) 10 mg/kg BW, (4) 100 mg/kg BW, and (5) 1000 mg/kg BW. Every treatment consisted of 5 mice. After receiving A.altissima extract for seven day consecutively, the mice were then euthanized with overdose of ether. The liver and kidney were taken and then fixed within 10% solution of Buffer Neutral Formalin (BNF). Histopathology slides of liver and kidney were processed and stained with Hematoxylline Eosin. The cell degeneration and cell death were observed with video photo microscope. The lesion of cell death and degeneration of hepatocytes and epithelium of kidney proximal tubule were counted. The data were analyzed statistically with ANOVA test continued with Duncan test. The result showed that cell necrosis of hepatocytes was not significantly different (P>0.05) compared to control treatment, while cell necrosis was increasing in the treatment of ≥ 10 mg/kg BW in tubuli of the kidney.
Co-Authors A.S. Satyaningtijas Arbi Mei Gitarama Bambang Pontjo Priosoeryanto Chairunnisa Chairunnisa Dwi Kesuma Sari Emma Sri Kuncari Hernomoadi Huminto Huda Shalahudin Darusman Kazuyuki Uchida Lies Parede Majariana Krisanti NASTITI KUSUMORINI Praptiwi Praptiwi Ryoji Yamaguchi Sri Estuningsih Sunarno . Susumu Tateyama Sutiastuti Wahyuwardani Vinka Aftinata Kusumaputri Wasmen Manalu