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All Journal HAYATI Journal of Biosciences Indonesian Journal of Biotechnology Indonesian Journal of Emerging Trends in Community Empowerment
Ence Darmo Jaya Supena
Department Of Biology, Faculty Of Mathematics And Natural Sciences, IPB University, Bogor 16680, Indonesia
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Education Immunology & microbiology Library & Information Science Materials Science & Nanotechnology

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Induction of Somatic Embryogenesis in Sengon (Falcataria moluccana) With Thidiazuron and Light Treatments Ari Sunandar; . Dorly; Ence Darmo Jaya Supena
HAYATI Journal of Biosciences Vol. 24 No. 2 (2017): April 2017
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (992.467 KB) | DOI: 10.4308/hjb.24.2.105

Abstract

Falcataria moluccana is important for reforestation and afforestation in Indonesia. However, epidemic of gall rust disease in F. moluccana plantations decreases its productivity. Genetic engineering is an alternative solution to against gall rust disease. Somatic embryogenesis is an efficient in vitro plant regeneration for successful plant improvement through genetic engineering. The objective of this study was to investigate the effect of thidiazuron and light treatments on the induction of somatic embryogenesis of F. moluccana. The effects of thidiazuron concentration (5, 10 or 15 μM) and light (continuous light, 7 days of dark followed by light, or continuous dark) on the induction of somatic embryogenesis in leaf explants were assessed. The highest production of somatic embryos was obtained in 5 μM thidiazuron and dark treatments for 7 days followed by light in Murashige and Skoog medium supplemented with 1.2 g/L proline. Histological analysis in globular and cotyledon stages confirmed that cells had progressed to secondary somatic embryogenesis. This research needs more improvements to become a successful and efficient somatic embryogenesis method and as a potential method for successful plant improvement through genetic engineering in F. moluccana.
Detection and quantification of splicing variants of Hd3a gene in oil palm Aqwin Polosoro; Wening Enggarini; Kusumawaty Kusumanegara; Toto Hadiarto; Miftahudin Miftahudin; Ence Darmo Jaya Supena
Indonesian Journal of Biotechnology Vol 29, No 1 (2024)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.88327

Abstract

Alternative splicing is a complex process that contributes to the generation of diverse mRNA and protein isoforms, including in oil palm (Elaeis guineensis). Despite their importance, many functions of alternative splicing genes remain poorly characterized. This study aims to investigate splicing variants of gene encoding Heading date 3a in E. guineensis (EgHd3a) using the GenBank database and ClustalW algorithm. To ensure the data accuracy and reliability of design isoform‐ specific primers, special emphasis is given to primer design techniques and validation using polymerase chain reaction (PCR) and quantitative real‐time (qRT)‐PCR analysis. The designed primers demonstrated high specificity and discrimination between mRNA specimens. Nucleotide variations at the 3’‐end influenced the specificity of primers with the addition of GC composition. Furthermore, qRT‐PCR analysis revealed a strong correlation between Ct values and gene concentration for the isoforms which indicates a reliable amplification of EgHd3a. Although two isoforms, Hd3a‐X2 and Hd3a‐X3, showed slightly higher than acceptable PCR efficiency values, caution is advised to prevent non‐specific amplification. Despite the challenge posed by the limitation of primer positioning due to alternative splicing, the chosen primer proved optimal for analysis. This study highlights the importance of considering alternative splicing in gene quantification experiments and provides insights into the critical steps, methods, and quality control measures necessary for accurately detecting alternative splicing events, contributing to understanding this complex biological process.
Workshop Manajemen dan Substansi Pengelolaan Jurnal Ilmiah Menuju Jurnal Terakreditasi Nasional dan Internasional Muhamad Syazali; Hayatul Khairul Rahmat; I Dewa Ketut Kerta Widana; Ence Darmo Jaya Supena; Rahmat Basuki; Miftahul Huda Fendiyanto; Adhi Kusumadjati; Mo Awwanah
Indonesian Journal of Emerging Trends in Community Empowerment Vol. 1 No. 1 (2023): Juni
Publisher : Perkumpulan Ahli Bimbingan dan Konseling Islam Indonesia

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Abstract

Workshop on the management and substance of managing scientific journals towards national and international accredited journals aim to gain an understanding of how the management and substance of management of scientific journals towards national and international accredited journals within the Faculty of Military Mathematics and Natural Sciences, the Republic of Indonesia Defense University. The procedure for implementing activities starts from the preparation, implementation, and closing stages. This activity is very useful for improving the quality of scientific journal management at the Faculty of Military Mathematics and Natural Sciences, the Republic of Indonesia Defense University towards national and international accredited journals. This can also be seen from the response and enthusiasm of the participants who asked when carrying out management workshop activities and the substance of managing scientific journals towards this national and international accredited journal.
Co-Authors Adhi Kusumadjati Aqwin Polosoro Dorly Dorly Hayatul Khairul Rahmat I Dewa Ketut Kerta Widana Kusumawaty Kusumanegara Miftahudin Miftahudin Miftahul Huda Fendiyanto Mo Awwanah Muhamad Syazali Rahmat Basuki Sunandar, Ari Toto Hadiarto Wening Enggarini