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KARAKTERISTIK ANATOMI DAUN DARI SEPULUH SPESIES Hoya SUKULEN SERTA ANALISIS HUBUNGAN KEKERABATANNYA Hafiz, Putra; Dorly, Dorly; Rahayu, Sri
Buletin Kebun Raya Vol 16, No 1 (2013): Buletin Kebun Raya Vol. 16 (1) Januari 2013
Publisher : Center for Plant Conservation Bogor Botanic Garden, Indonesian Institute of Sciences

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Abstract

Hoya has two types of leaves, succulent and non succulent. Succulent plant adapted well in extreme conditions, especially during dry period. Anatomical characters can be used to identify, classify, and determine plant relationship. The aims of this research were to identify anatomical character of succulent Hoya’s leaf and to determine their relationship. Ten examined species Hoya spp. (H. diversifolia, H. latifolia, H. dolichosparte, H. bilobata, H. lacunosa, H. verticillata, H. purpureofusca, H. kuhlii, H. oblanceolata, and H. micrantha) showed anatomical similarity in their epidermal layer, hipodermal, mesophyl, and the absence of sponge tissue at mesophyl. However, there are diversities among the species. Based on paradermal section of the leaf,  two types of stomata were only found at the abaxial side, individual stomata and both individual and stomatal cluster. Hoya diversifolia has the smallest stomatal size, while Hoya latifolia has the biggest. Hoya lacunosa has the lowest stomatal density, while Hoya bilobata has the highest. Hoya diversifolia, Hoya lacunosa, and Hoya oblanceolata have the lowest stomatal index, while Hoya bilobata has the highest. Observation on transversal section showed that Hoya purpureofusca has the lowest leaf thickness, while Hoya kuhlii has the highest. The hierarchical cluster analysis based on anatomical leaf characters showed different patterns of relationship which diversed as three groups at relationship scale 15.
KERAGAMAN DAN ANALISIS KEKERABATAN Hoya spp. BERTIPE DAUN NON SUKULEN BERDASARKAN KARAKTER ANATOMI DAUN Hakim, Aldi Rahman; Dorly, Dorly; Rahayu, Sri
Buletin Kebun Raya Vol 16, No 1 (2013): Buletin Kebun Raya Vol. 16 (1) Januari 2013
Publisher : Center for Plant Conservation Bogor Botanic Garden, Indonesian Institute of Sciences

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Abstract

Hoya spp. (Apocynaceae: Asclepiadoideae) has differences in leaf type, there are succulent and non succulent leaf types.  Anatomical structure of Hoya spp. leaf has not been widely studied, especially for the non succulent type. The aims of this research were to explore the diversity and relationship of non succulent Hoya, based on leaf anatomical characters represented by eight species i.e. H. bandaensis, H. campanulata, H. chlorantha, H. cilliata, H. coriacea, H. coronaria, H. densifolia and H. multiflora. Hoya leaf anatomical characters were observed on the paradermal and transversal section and analyzed by using IBM SPSS version 19 sotfware for cluster analysis. According to the paradermal observation, stomata were present at lower surface (hypostomatic) for all species, and amphistomatic (both surface) for H. densifolia.  The type of stomata is cyclocytic for all species. Clustered stomata were found in H. coriacea.  Observation on transversal section showed that all of species has the normal structure i.e cuticula, upper and lower epidermis, palisade parenchyma and spongy parenchyma with the variation in the layer thickness.  The cluster analysis resulted four groups at distance scale 19. Each group has specific characters. The first group has trichomes on both sides of the leaf surface. The second group has amphistomatic stomatal. The third group has thin leaves. The fourth group has the widest stomata.
KARAKTERISTIK ANATOMI DAUN DARI SEPULUH SPESIES Hoya SUKULEN SERTA ANALISIS HUBUNGAN KEKERABATANNYA Hafiz, Putra; Dorly, Dorly; Rahayu, Sri
Buletin Kebun Raya Vol 16, No 1 (2013): Buletin Kebun Raya Vol. 16 (1) January 2013
Publisher : Center for Plant Conservation Bogor Botanic Garden, Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Hoya has two types of leaves, succulent and non succulent. Succulent plant adapted well in extreme conditions, especially during dry period. Anatomical characters can be used to identify, classify, and determine plant relationship. The aims of this research were to identify anatomical character of succulent Hoya’s leaf and to determine their relationship. Ten examined species Hoya spp. (H. diversifolia, H. latifolia, H. dolichosparte, H. bilobata, H. lacunosa, H. verticillata, H. purpureofusca, H. kuhlii, H. oblanceolata, and H. micrantha) showed anatomical similarity in their epidermal layer, hipodermal, mesophyl, and the absence of sponge tissue at mesophyl. However, there are diversities among the species. Based on paradermal section of the leaf,  two types of stomata were only found at the abaxial side, individual stomata and both individual and stomatal cluster. Hoya diversifolia has the smallest stomatal size, while Hoya latifolia has the biggest. Hoya lacunosa has the lowest stomatal density, while Hoya bilobata has the highest. Hoya diversifolia, Hoya lacunosa, and Hoya oblanceolata have the lowest stomatal index, while Hoya bilobata has the highest. Observation on transversal section showed that Hoya purpureofusca has the lowest leaf thickness, while Hoya kuhlii has the highest. The hierarchical cluster analysis based on anatomical leaf characters showed different patterns of relationship which diversed as three groups at relationship scale 15.
KERAGAMAN DAN ANALISIS KEKERABATAN Hoya spp. BERTIPE DAUN NON SUKULEN BERDASARKAN KARAKTER ANATOMI DAUN Hakim, Aldi Rahman; Dorly, Dorly; Rahayu, Sri
Buletin Kebun Raya Vol 16, No 1 (2013): Buletin Kebun Raya Vol. 16 (1) January 2013
Publisher : Center for Plant Conservation Bogor Botanic Garden, Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Hoya spp. (Apocynaceae: Asclepiadoideae) has differences in leaf type, there are succulent and non succulent leaf types.  Anatomical structure of Hoya spp. leaf has not been widely studied, especially for the non succulent type. The aims of this research were to explore the diversity and relationship of non succulent Hoya, based on leaf anatomical characters represented by eight species i.e. H. bandaensis, H. campanulata, H. chlorantha, H. cilliata, H. coriacea, H. coronaria, H. densifolia and H. multiflora. Hoya leaf anatomical characters were observed on the paradermal and transversal section and analyzed by using IBM SPSS version 19 sotfware for cluster analysis. According to the paradermal observation, stomata were present at lower surface (hypostomatic) for all species, and amphistomatic (both surface) for H. densifolia.  The type of stomata is cyclocytic for all species. Clustered stomata were found in H. coriacea.  Observation on transversal section showed that all of species has the normal structure i.e cuticula, upper and lower epidermis, palisade parenchyma and spongy parenchyma with the variation in the layer thickness.  The cluster analysis resulted four groups at distance scale 19. Each group has specific characters. The first group has trichomes on both sides of the leaf surface. The second group has amphistomatic stomatal. The third group has thin leaves. The fourth group has the widest stomata.
Induksi Pembungaan Rambutan dengan Aplikasi Paklobutrazol Prawitasari, Theresia; Dorly, Dorly; Wahyuni, Sri
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 2 (2005): June 2005
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (351.556 KB) | DOI: 10.24002/biota.v10i2.2845

Abstract

Rambutan fruit have a great potential to be developed as an exported commodity. The constraint is the existence of biannual bearing characteristic of the plant, which is an alternation of fruit yield between on year and off year. This condition resulted in a problem in product availability continuity. The aim of this research was to induce rambutan plant flowering in the off year with the application of paclobutrazol. There were three doses of paclobutrazol treatment including 0 g/tree, 1.5 g/tree, and 3 g/tree. Bud dormancy as an implication of paclobutrazol application were broken by the application of KNO3 at 20 g/l concentration. The experiment was arranged in completely randomized factorial design 3X2 in triplicate. The result showed that paclobutrazol treatment was able to induce rambutan flowering in the off year. Paclobutrazol treatment significantly increased the number of generative buds. Based on the generative bud percentage, the higher doses of paclobutrazol application the stronger stimulus of flowering. Paclobutrazol treatment also reduced the inflorescences length and increased the chlorophyll content of the flowering support leaves. It seems that application at dose 3 g/tree caused bud dormancy but dose 1.5 g/tree did not. Bud dormancy as a result of paclobutrazol application could be broken by the KNO3 application. The optimum dose to induced rambutan flowering was 1.5 g/tree because this dose seems did not caused bud dormancy and gave a higher number of generative buds compared to 3 g/tree dose.
Studi Anatomi Daun dari Tiga Anggota Suku Malvaceae di Kawasan Waduk Jatiluhur Dorly, Dorly; Ningrum, Ratih Kusuma; Suryantari, Ni Kadek; Anindita, Fawzia La Rizma
Proceeding Biology Education Conference: Biology, Science, Enviromental, and Learning Vol 13, No 1 (2016): Prosiding Seminar Nasional XII Biologi
Publisher : Universitas Sebelas Maret

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Abstract

Jatiluhur reservoir natural resort has miscellaneous vegetation, one of them is shrubs vegetation mostly found Malvaceae family.  The purpose of this research was to study the diversity of anatomical structure of three members of Malvaceae family found in Jatiluhur reservoir area.  Samples were leaf part of pungpulutan (Urena lobata), pulutan (Sida glutinosa), and sidaguri (Sida rhombifoliaa).  Anatomical observations were carried out on the paradermal and transversal thin sections of microscopic preparation. Observation results of leaf paradermal preparation  of the three plant species were anomositic stomata type were found on both sides of leaf (amphistomatic) with higher density on abaxial side than on adaxial side.  Pungpulutan and pulutan have the same epidermis cell shape, wavy, while sidaguri has polygonal epidermis cell shape.  On pungpulutan and pulutan were found non-glandular  trichome type, whereas on sidaguri were discovered non-glandular and glandular trichome types. Observation results of leaf transversal preparation of three kinds of plants was found a layer of epidermis tissue.  Palisade tissue of pungpulutan consisted of a layer of cells, while on pulutan and sidaguri the palisade tissue consisted of 3-5 layers of cells and 4-6 layers of cells subsequently.
Secretory Duct Structure and Phytochemistry Compounds of Yellow Latex in Mangosteen Fruit DORLY DORLY; SOEKISMAN TJITROSEMITO; ROEDHY POERWANTO; JULIARNI JULIARNI
HAYATI Journal of Biosciences Vol. 15 No. 3 (2008): September 2008
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (293.533 KB) | DOI: 10.4308/hjb.15.3.99

Abstract

Yellow latex is the main problem in mangosteen agribusiness, because it is one factor lowering the fruit quality. The structure of yellow latex secretory ducts in the flower and fruit as well as in the root, stem and leaf of mangosteen (Garcinia mangostana L.) seedling and the qualitative phytochemistry of yellow latex were studied. The ducts were branched, canal-like type. They were found in the exocarp, mesocarp, endocarp, aril of the fruit, flower, stem, and leaf. In the fruit, the biggest diameter of the secretory ducts was found in the endocarp. There were continuous secretory ducts from fruit stalk to the fruit. Ultrastructural observation showed that the ducts surrounded by specific epithelial cells, which were living cells containing dense cytoplasm with plastid, mitochondria and golgi apparatus organelles. The qualitative test indicated that the yellow latex collected from stem bark, outer part of fruit, young fruit pericarp, mature aril and young aril contained terpenoid, flavonoid and tannin, but not alkaloid, saponin and steroid, except in the young aril containing the steroid. Key words: secretory ducts, yellow latex, endocarp, aril, epithelial cells
Induction of Somatic Embryogenesis in Sengon (Falcataria moluccana) With Thidiazuron and Light Treatments Ari Sunandar; . Dorly; Ence Darmo Jaya Supena
HAYATI Journal of Biosciences Vol. 24 No. 2 (2017): April 2017
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (992.467 KB) | DOI: 10.4308/hjb.24.2.105

Abstract

Falcataria moluccana is important for reforestation and afforestation in Indonesia. However, epidemic of gall rust disease in F. moluccana plantations decreases its productivity. Genetic engineering is an alternative solution to against gall rust disease. Somatic embryogenesis is an efficient in vitro plant regeneration for successful plant improvement through genetic engineering. The objective of this study was to investigate the effect of thidiazuron and light treatments on the induction of somatic embryogenesis of F. moluccana. The effects of thidiazuron concentration (5, 10 or 15 μM) and light (continuous light, 7 days of dark followed by light, or continuous dark) on the induction of somatic embryogenesis in leaf explants were assessed. The highest production of somatic embryos was obtained in 5 μM thidiazuron and dark treatments for 7 days followed by light in Murashige and Skoog medium supplemented with 1.2 g/L proline. Histological analysis in globular and cotyledon stages confirmed that cells had progressed to secondary somatic embryogenesis. This research needs more improvements to become a successful and efficient somatic embryogenesis method and as a potential method for successful plant improvement through genetic engineering in F. moluccana.
Studi Pemberian Kalsium untuk Mengatasi Getah Kuning pada Buah Manggis (Garcinia mangostana L.) , Dorly; Indah Wulandari; Roedhy Poerwanto; Darda Efendi; Soekisman Tjitrosemito
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 39 No. 1 (2011): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (193.751 KB) | DOI: 10.24831/jai.v39i1.13188

Abstract

Gamboge is the main problem in mangosteen agribusiness because it is one of the major factors lowering fruit quality. Calcium is one of the important elements that strengthening cell wall; it is which was binding with the pectin as a middle lamella component. The objectives of the research were to study the effect of calcium application on the presence of gamboge spots, physical, and chemical properties of mangosteen fruit. Trial was conducted in a randomized complete block design with three replications in two consecutive years. The calcium source was dolomite, applied in four different dosages, i.e. 0, 18, 24, and 34 ton ha-1 for the fi rst year and 0, 12.5, 15, and 17.5 ton ha-1 for the second year. The results showed that calcium application raised soil pH and calcium content of the soil, exocarp and mangosteen leaves. Dolomite applications using 18 and 24 ton ha-1 in the fi rst year and 17.5 ton ha-1 in the second year were effective to reduce gamboge spots on the outer part of fruit, however they were not effective to reduce gamboge in aril. Dolomite applications did not increase transversal diameter, fruit weight, total soluble solids, total titratable acids, and ratio of total soluble solids with total titratable acids of the fruits.
Struktur Sekretori Tanaman Bahan Ramuan Obat Diabetes . Dorly
Jurnal Ilmu Pertanian Indonesia Vol. 11 No. 1 (2006): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

Anatomical structure of medicinal plants wich are used in diabetic therapy is not yet completely identified. This research was conducted to study the structure of their secretory tissues. Observation of anatomical structure of leaves was done by making paradermal and transversal sections, while observation of anatomical structure of stem, fruits, and rhizomes were performed by making transversal sections. Microscopic observation found several types os secretory structure in plant organs observed. Glandular hairs were found in kiurat (Plantago mayor L.) leaves, sambiloto (Andrographis paniculata (Burm.f) Wallich ex Nees) leaves, and kumis kucing (Orthosiphon aristatus (Blume) Miq.) leaves; lithocysts cell were found in sambiloto leaves; oil cavity/cells were found in salam (Syzygium polyanthim (Wight) Walp.) leaves, jambu (Psidium guajava L.) leaves, lada (Piper nigrum L.) fruits, and jahe (Zingiber officinale Roscoe) rhizomes; idioblast cells wich contained starch grains were found in brotowali (Tinospora crispa (L.) Miers) stems, lada fruits, and jahe rhizomes; and latex cells were found in brotowali stems.