The growing concerns over ozone depletion and global warming caused by refrigerants have led to the search for environmentally friendly alternatives. This study evaluates the impact of varying R-290 refrigerant charge masses on the performance of a wall-mounted residential air conditioner using the drop-in substitute method. A ¾ HP residential AC unit originally charged with 550 grams of R-22 refrigerant was retrofitted with R-290 and tested at charge masses of 140 grams, 165 grams, and 190 grams—approximately 25%, 30%, and 35% of the original R-22 charge, in accordance with the commonly applied “one-third rule.” The results showed that retrofitting with R-290 increased the Refrigeration Effect (RE) by up to 75%, Compression Work (Wc) by 68%, and Coefficient of Performance (COP) by up to 18%. The system with a 25% refrigerant charge was unable to reach the set temperature due to a 23% reduction in cooling capacity, while the 30% charge showed a 10% reduction. The 35% refrigerant mass retrofit proved the most suitable, achieving adequate cooling capacity, an 18% increase in COP, and a 14% reduction in power consumption. Additionally, the retrofit resulted in an indirect CO₂ emission reduction of 1.15 metric tons annually, highlighting the environmental and energy-saving advantages of using R-290. These findings provide empirical validation of the one-third rule for refrigerant mass variation in R-290 retrofits and offer valuable insights into optimizing performance and efficiency in residential AC units, with significant energy and environmental benefits.