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All Journal Indian Journal of Forensic Medicine & Toxicology
Devanshi Gosai
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Public Health

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Effect of Grey Zone Samples Testing by Enzyme Immunoassay and Its Impact on Enhancing Blood Safety: Experience from Tertiary Care Hospital based Blood Bank in Central Gujarat Ashu Dogra; Devanshi Gosai; Jasmin Jasani
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 2 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i2.14280

Abstract

Background : Enzyme linked immunosorbent assay is a standard protocol adopted by majority of BloodBanks for screening of blood units for transfusion transmissible infections. The grey zone testing of samplesincreases sensitivity of ELISA tests which in turn enhances the safe blood transfusion practices. The presentstudy was thus designed to know the effect of repeat testing of Grey zone Blood Donor samples forimproving the sensitivity of ELISA Screening methods in blood banks.Methods:- All Blood Donor Samples which were 10% below the cut- off value were marked as Grey zonesamples and were repeated for ELISA screening . Interpretation of Repeat tests was done as per NACO andWHO guidelines and the samples which recorded their one or both O.D value above cut- off were labelledas reactive and samples which recorded one or both O.D value in grey zone were marked as indeterminate.Results and Conclusion: In present study, a total of 10,425 Blood Donors were screened for TTI’ s andout of 10,425 (15) Blood donor samples were found in grey zone. The results of repeat testing showed that9 (60%) Blood Donor samples were found to be confirm reactive for TTI. Grey zone sample testing shouldbe done routinely as a measure to enhance sensitivity of ELISA testing of Blood Donors in developingcountries.
Utility of the Cell Block Preparation as an Additive Tool to the Conventional Cytological Smears Devanshi Gosai; Kuntal Patel; Ashu Dogra; Jasmin Jasani
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 2 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i2.14286

Abstract

Background: For the detection of malignancy in the serous fluids of the body, cytological examination ofthe fluid is very important. It is also of prime importance and seems difficult to differentiate the malignantcells from reactive mesothelial cells during fluid cytology. The cell block preparation is a useful and additivediagnostic tool when cellular features in cytology are inconclusive or suspicious. The aim of the study wasto assess the utilization of the cell technique as an additional tool along with the conventional smears thatwe are using in routine practice.Methods: 200 body (effusion) fluids were examined by the conventional cytological smears as well as bythe cell block technique.Conclusion: The diagnostic yield increases when we use the cell block preparation method along with theconventional smears because the architectural patterns and cell morphology are very well preserved in cellblock as compared to the smears.
Co-Authors Ashu Dogra Jasmin Jasani Kuntal Patel