<![CDATA[Background : Enzyme linked immunosorbent assay is a standard protocol adopted by majority of BloodBanks for screening of blood units for transfusion transmissible infections. The grey zone testing of samplesincreases sensitivity of ELISA tests which in turn enhances the safe blood transfusion practices. The presentstudy was thus designed to know the effect of repeat testing of Grey zone Blood Donor samples forimproving the sensitivity of ELISA Screening methods in blood banks.Methods:- All Blood Donor Samples which were 10% below the cut- off value were marked as Grey zonesamples and were repeated for ELISA screening . Interpretation of Repeat tests was done as per NACO andWHO guidelines and the samples which recorded their one or both O.D value above cut- off were labelledas reactive and samples which recorded one or both O.D value in grey zone were marked as indeterminate.Results and Conclusion: In present study, a total of 10,425 Blood Donors were screened for TTI’ s andout of 10,425 (15) Blood donor samples were found in grey zone. The results of repeat testing showed that9 (60%) Blood Donor samples were found to be confirm reactive for TTI. Grey zone sample testing shouldbe done routinely as a measure to enhance sensitivity of ELISA testing of Blood Donors in developingcountries.]]>
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