V.P. Bintoro
Faculty of Animal Agriculture, Diponegoro University Tembalang Campus, Semarang 50275, Central Java

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MICROBIOLOGICAL PROPERTIES OF BEEF IN VARIOUS MEAT SHOPS AT SEMARANG, INDONESIA Nurwantoro, N.; Bintoro, V.P.; Legowo, A.M.; Purnomoadi, A.
Journal of the Indonesian Tropical Animal Agriculture Vol 37, No 2 (2012): (June)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.37.2.97-102

Abstract

The aim of the study was to assess microbiological properties of beef sold in various meatshops in Semarang. There are five Indonesian goverment standard requirements to maintain the qualityof beef sold in Indonesia markets, as follows: (1) total plate count (TPC) for a maximum of 106 CFU/g,(2) total coliform bacteria for a maximum of 102 CFU/g, (3) total Escherichia coli up to 10 CFU/g, (4)total Staphylococcus aureus for a maximum of 102 CFU/g and (5) negative for Salmonella per 25 gsamples (SNI 3932, 2008). Beef samples were randomly taken from several traditional markets, meatshops and supermarkets. The result showed that all samples did not contain Salmonella but still couldnot meet one or some of the Indonesian government standard regulation. In conclusion, beef samplesgathered from some of the markets, generaly could not meet one or some of the five strictlyrequirements of the bacteriological properties.
MICROBIOLOGICAL AND PHYSICAL PROPERTIES OF BEEF MARINATED WITH GARLIC JUICE Nurwantoro, N.; Bintoro, V.P.; Legowo, A.M.; Ambara, L.D.; Prakoso, A.; Mulyani, S.; Purnomoadi, A.
Journal of the Indonesian Tropical Animal Agriculture Vol 36, No 3 (2011): (September)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.36.3.166-170

Abstract

The study was carried out to investigate the effect of marination, performed by soaking of beef ingarlic juice, on microbiological and physical properties. The study was committed to a completelyrandomized design, with 5 treatments, i.e.: T0 (unmarinated beef, as a control), T1, T2, T3, and T4 thatbeef were marinated in garlic juice for 5, 10, 15, and 20 minutes, respectively, at room temperature(25⁰C). Each treatment consisted of 4 replications. Examination upon experimental parameters wasconducted after marinated (and control) beef was stored for 8 hours at room temperature. Total bacteria,total coliform and water holding capacity of beef were significantly (P<0.05) affected by marinationwith garlic juice. Conversely, cooking loss was not significantly affected (P>0.05) by the treatments. Asa conclusion, marination of beef with garlic juice could reduce total bacteria, total coliform, and waterholding capacity, but could not reduce cooking loss.
THE SOAKING EFFECT ON DIFFERENT HYDROCLORIDE ACID LEVEL AND SOAKING TIME ON PH, SWELLING PERCENTAGE AND COLLAGEN YIELD OF CHICKEN SHANK BONE Puspitasari, D.A.P.; Bintoro, V.P.; Setiani, B.E.
Journal of the Indonesian Tropical Animal Agriculture Vol 38, No 2 (2013): (June)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.38.2.98-102

Abstract

Collagen is the main ingredient in the gelatin manufacture. The purpose of the study was todetermine the pH value, the swelling percentage and collagen yield of chicken shank bones which weresoaked by different HCl concentration and soaking time. The pH value and swelling percentage ofchicken shank bones are indicator that determines amount of collagen yield. The research design usedwas factorial completely randomized design (CRD). Factor A was a concentration of HCl (2%, 3.5% and5%) and factor B was a soaking time (24, 36 and 48 hours) without replication (profile). The resultshows that the the increase of HCl concentration and soaking time affected on the decreasing of pHvalue profile (0.17-0.85), swelling percentage profile (80.25-97.49%) and also collagen yield (1.80-10.02%). The lowest collagen yield of chicken shank bones was suspected caused by the used of Hcl.The highest result of the study was using 2% of HCl concentration for 24 hours. The best result wasfound in the use of 2% HCl and was soaked for 24 hours. Based on those result, the collagen of chickenshank bones was recommended as a main ingredient for gelatin manufacture.