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All Journal PROSIDING SEMINAR NASIONAL Jurnal Labora Medika Jurnal Biomedika Jaringan Laboratorium Medis Prosiding Seminar Nasional Unimus
Arya Iswara
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Humanities Biochemistry, Genetics & Molecular Biology Education Engineering Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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Journal : PROSIDING SEMINAR NASIONAL

 1 
POLA SENSITIVITAS Eschericia coli TERHADAP ANTIBIOTIK METRONIDAZOLE Arya Iswara
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2015: Prosiding Bidang MIPA dan Kesehatan The 2nd University Research Colloquium
Publisher : Universitas Muhammadiyah Semarang

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Abstract

Escherichia coli (E. coli) have a potency to be a pathogen bacteria cause an illness. Antibiotic treatments to a patient have a purpose to eliminate the pathogen bacteria. Bacteria resistance to antibiotic was influenced by the intensity of antibiotic treatment in a region, the uncontrolled antibiotics treatments would increase the antibiotic resistance of bacteria. This research was using four different sample, they were feces from diarrhea, ice block, waters from well, and ketchup. Approximately 1 gram from each sample diluted in 10 mL of BHI and then incubated for 6 hours in 37 C. After 6 hours incubations, the samples inoculated in EMBA and incubated in 37 C for 24 hours. The metallic green colored bacteria colony inoculated in Mac Conkey Agar and incubated in 370C for 24 hours. The separated colony sub cultured into HIA and tested using biochemistry test. Sensitivity test conducted using the Kirby Bauer method. The test result showed that 5 samples were sensitive to metronidazole and 26 samples wereresistant to metronidazole.Key word: E.coli, metronidazole, sensitivity.
EFEKTIVITAS INFUSA KULIT JERUK PURUT (Citrus hystrix DC.) TERHADAP PERTUMBUHAN Candida albicans PENYEBAB SARIAWAN SECARA in vitro Zakiyatul Khafidhoh; Sri Sinto Dewi; Arya Iswara
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2015: Prosiding Student Paper Presentation The 2nd University Research Colloquium
Publisher : Universitas Muhammadiyah Semarang

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Candida albicans is a normal flora in the oral mucosa, tongue, and palate, but it could become the pathogenic one. If the amounts were excess so that it could cause thrush. Kaffir lime peel infusion contains saponins, tannins, flavonoids, coumarin which are active antifungal compound. The purpose of this study was to examine the effectiveness of kaffir lime peel infusion to the growth of Candida albicans which causes thrush with a consentration of 10%, 15%, 20% and contact time of 5 minutes, 10 minutes, 15 minutes. Method of this study used an experimental research laboratory. Suspension Candida albicans 100 mL with dilution 10-4 of McFarland 0,5 and contacted to the kaffir lime peel infusion 10%, 15%, 20% with three repetitions. After 5 minutes, 10 minutes, and 15 minutes put 100 μL and then inoculated into SGA antibiotics then incubated at 37˚C for 48 hours. Based on the results of this study concluded that kaffir lime peel infusion can inhibit the growth of Candida albicans. The best results was the 20% consentration and contact time of 15 minutes with an average colonies of 3×104 CFU/100 μL. The higher consentration of kaffir lime peel infusion the more able to inhibit the growth of Candida albicans and the longer the contact time kaffir lime peel infusion, the more can inhibit the growth of Candida albicans.Keywords: Infusion, Citrus hystrix DC., Candida albicans.
EFEK PEMBERIAN Morinda citrifolia,L PADA KADAR TGF-Β SERUM DAN EKSPRESI KOLAGEN PADA GINJAL TIKUS DIABETES NEFROPATI Arya Iswara; Udadi Sadana; Indranila Kustarini
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2014: PROSIDING SEMINAR NASIONAL HASIL - HASIL PENELITIAN & PENGABDIAN
Publisher : Universitas Muhammadiyah Semarang

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Background: Morinda citrifolia L contain components that lowering the oxidative stress andthe ability to regenerate the renal function through the measurement of renal extra cellularmatrix and growth factor. The detection from renal functions remission can be measuredthrough the transforming growth factor beta (TGF-β) level, and collagen deposition. Aims: This experiment was to prove the Morinda citrifolia L effect to lowering the TGF-β level andcollagen deposition in streptozotocin-induced nephropathy diabetic Sprague Dawley rats.Method:  The post test only control group design experiment in thirty rats randomized intopositive control group and four treatment group and induced with streptozotocin in 40mg/kgWBdoses. The rats treated with Morinda citrifolia extract which was divided into four doses(10;20;40;80mg/dL) then determine TGF-β level and collagen deposition. The data analyzedusing SPSS ver 17 with oneway-ANOVA and post hoc LSD, Kruskal-Wallis and Mann-WhitneyU and Spearman’s Rho with confidence interval 95% (p<0,05). Result: The statisticallyanalysis from rats treated with morinda extract resulted a significant result in TGF-β level, correlation between TGF-β level and collagen deposition (p<0,05). Conclusion: Morindacitrifolia treatment can substantially improve the activity of the renal function, shown by lowerTGF-β level and better density of collagen deposition compared with control group and 10-20mg/dL morinda treatment dose showed the best result.Keywords: Nephropathy diabetics, Morinda citrifolia, TGF-β level and collagen deposition.
VARIASI KONSENTRASI KOH DAN WAKTU CLEARING TERHADAP KUALITAS PREPARAT AWETAN Pediculus humanus capitis Arya Iswara; Fitri Nuroini
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2017: Prosiding Seminar Nasional Publikasi Hasil-Hasil Penelitian dan Pengabdian Masyarakat
Publisher : Universitas Muhammadiyah Semarang

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Abstract

Pembuatan sediaan permanen entomologi/insekta diawali dengan perendaman dalam KOH, dilanjutkan dengan proses dehidrasi, proses clearing dan proses mounting. Perendaman dalam KOH bertujuan menipiskan lapisan kitin pembentuk eksoskeleton pada insekta. Tahapan Clearing merupakan salah satu tahapan pembuatan awetan permanen yang bertujuan menjadikanstruktur parasit insekta terlihat lebih jelas, jernih, dan transparan. Penelitian dilakukan dengan variasi perendaman dalam larutan KOH 5%, 10%, 15%, dan 20%. selama 24 jam, serta variasi lama waktu clearing 5, 15, 25, dan 60 menit. Pengamatan sediaan awetan permanen dilakukan dengan menilai kualitas sediaan awetan permanen. Kualitas sediaan awetan permanen meliputi kejernihan, kualitas warna, dan keutuhan  sediaan awetan permanen. Hasil penelitian menunjukkan hasil yang bervariasi, kualitas yang buruk didapatkan pada kombinasi antara variabel waktu clearing 5 menit pada seluruh variasi konsentrasi KOH dan pada variabel waktu clearing 15menit pada konsentrasi KOH 5%. Hasil dengan kualitas baik ditunjukkan pada variabel waktu clearing 15 menit pada konsentrasi KOH 10%, 15%, dan 20%, serta pada variabel vaktu clearing 25 dan 60 menit pada seluruh variasi konsentrasi KOH. Hasil kualitas yang baik disertai dengan peningkatan dan penurunan nilai skoring. Hasil dari penelitian ini dapat ditarik kesimpulan bahwa semakin lama waktu clearing akan menghasilkan preparat yang lebih baik dengan nilai skoring yang berbeda berdasarkan persentase KOH yang digunakan.Keywords: KOH, clearing, Pediculus humanus capitis
Co-Authors Djoko Priyatno Eko Naning Sofyanita Fitri Nuroini Gela Setya Ayu Putri Indranila Kustarini Muhamad Jumardi Sri Sinto Dewi Tulus Ariyadi Udadi Sadana Yulfa Ariza Masruro Zakiyatul Khafidhoh