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All Journal International Journal of Public Health Science (IJPHS) SAINS MEDIKA : JURNAL KEDOKTERAN DAN KESEHATAN Jurnal Riset Kesehatan Jurnal Surya Medika Jurnal Labora Medika JURNAL ANALIS LABORATORIUM MEDIK Jaringan Laboratorium Medis ARTERI : Jurnal Ilmu Kesehatan Borneo Journal of Medical Laboratory Technology Abdimasku : Jurnal Pengabdian Masyarakat Jurnal Health Sains Aurelia: Jurnal Penelitian dan Pengabdian Masyarakat Indonesia Jurnal Pengabdian Pada Masyarakat Gema Lingkungan Kesehatan Jurnal Dunia Ilmu Kesehatan Jurnal Laboratorium Khatulistiwa Poltekita: Jurnal Ilmu Kesehatan Jurnal Sains dan Kesehatan
Eko Naning Sofyanita
Department Of Medical Laboratory Technology, Poltekkes Kemenkes Semarang, Semarang, Central Java, Indonesia
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Tingkat Pengetahuan terhadap Donor Darah pada Masyarakat Pendonor Dina Afrianti; Elis Susilawati; Eko Naning Sofyanita
Jaringan Laboratorium Medis Vol 4, No 1 (2022): May 2022
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v4i1.8445

Abstract

Blood donation is an activity to donate blood for transfusion purposes. The number of voluntary blood donors in Indonesia is around 20% of the total production of blood bags per year, the rest is met from replacement donors. People are generally reluctant to donate blood because of fear of pain when blood is drawn using a syringe, fear of running out of blood, resulting in anemia, contracting the disease through blood donors, feeling unwell to donate blood and feeling that it is enough to only donate blood once. This is a factor that causes the limited number of donors due to limited knowledge. Research Objectives: To see an overview of the level of knowledge about blood donors in the community of donors at Pekalongan City. Methods: The type of research used is descriptive quantitative with a cross sectional approach with a questionnaire to determine the level of knowledge of the donor community. Results: Most of the blood donors at the Pekalongan City are Male 73 respondents (73%), the highest percentage aged 17-30 years as many as 54 respondents (54%), high school education, namely as many as 49 respondents (49%). Most of the donor knowledge level is said to be good, namely 88 respondents (88%). Conclusion: Most of the donor community at the Pekalongan City have a good level of knowledge about blood donation.
Rasio Penutupan Luka pada Tikus Diabetes Diinduksi Streptozotocin dengan Perlakuan Dressing Tipe Pasif dan Interaktif (Penelitian Pendahuluan) Eko Naning Sofyanita; Arya Iswara
Jaringan Laboratorium Medis Vol 3, No 2 (2021): November 2021
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v3i2.8045

Abstract

Deparaffinization is a stage before the staining process to remove/dissolve paraffin so that the absorption of color in tissue preparations is maximized. Deparaffinization is usually carried out using xylol and toluol. Xylol has toxic effects including acute neurotoxicity, heart and kidney damage, hepatotoxicity, fatal blood dyscrasias, skin erythema, dry skin, peeling skin, and also has a carcinogenic effect. The toxicity effect of olive oil is lower than that of xylol. Oils that have non-polar properties can remove the remaining paraffin contained in the tissue. The purpose of this study was to determine the microscopic appearance of the kidney tissue preparations of mice deparaffinized with olive oil on hematoxylin eosin (HE) staining. The type of research used is experimental research which is analyzed with a descriptive approach. The results of the assessment of preparations deparaffinized with xylol in 80 visual fields obtained 100% good preparations and preparations deparaffinized with olive oil in 80 visual fields obtained 0% poor preparations, 11.3% poor preparations, and 88.7% good preparation. So it can be said that better results are found in the microscopic picture of the kidney preparations of mice (Mus musculus) deparaffinized with xylol.
Hubungan Kadar Hemoglobin dan Kadar Kreatinin Darah pada Pasien Gagal Ginjal Kronik Pasca Transfusi Berulang Eko Naning Sofyanita; Roni Afriansya; Nur Indah Palupi
Jaringan Laboratorium Medis Vol 2, No 2 (2020): November 2020
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v2i2.7030

Abstract

Kidneys are part of the body that has an important role. One of the functions of the kidney is the production of erythropoietin. Erythropoietin stimulates the production of red blood cells. People or patients who have kidney problems can cause anemia. This study aims to determine the relationship between hemoglobin levels and blood creatinine levels in patients with chronic kidney disease after receiving a transfusion. Blood creatinine levels in this study were used as an index to measure kidney function. The study was conducted at the Wira Tamtama Hospital Semarang by taking data on 20 patients in 2020 with chronic kidney disease and taking data on hemoglobin (cut-off 12 g/dL) and serum creatinine (cut-off 1.5 mg/dL). The relationship between the two was calculated by chi-square test and found 80% of patients with low hemoglobin in patients with high creatinine levels and 0% of patients with low hemoglobin in patients with normal creatinine levels (p=0.040). There is a risk of decreased hemoglobin levels (OR = 3,442) in patients with high creatinine levels. It was concluded that there was a significant relationship between hemoglobin levels and blood creatinine levels and patients with high creatinine levels tended to be at risk of anemia.
Gambaran Ureum dan Kreatinin pada Pasien Penyakit Ginjal Kronik yang Menjalani Hemodialisis Roni Roni Afriansya; Eko Naning Sofyanita; Suwarsi Suwarsi
Jaringan Laboratorium Medis Vol 2, No 1 (2020): May 2020
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v2i1.5813

Abstract

Chronic Kidney Disease is evident if the blood urea level is more than 200 mg/dl. Uremia causes a malfunction in almost all organ systems such as; fluid and electrolyte disorders, metabolic endocrine, neuromuscular, cardiovascular and pulmonary, skin, gastrointestinal, hematological, and immunological. Hemodialysis is an attempt to reduce the symptoms of uremia so that the patient's clinical condition can also improve. The purpose of this study was to determine the description of Ureum and Creatinine in CKD Patients undergoing Hemodialysis. This type of research is observational descriptive. The sample included all CKD patients undergoing hemodialysis at the Ir Sukarno Sukoharjo Regional Hospital in 2019 who met the inclusion criteria, so as many as 83 samples were obtained. The type of data is secondary data obtained from medical records. The results showed that most patients were in the 40-60 years age group of 72% and the majority of the male sex were 51 patients (61%). Urea and creatinine appearance in patients undergoing hemodialysis has increased very high. In 83 patients with CKD increased serum creatinine levels ( 100%) with a mean creatinine level in men of 11.80 mg / dL and women of 9.73 mg / dL and an increase in ureum levels with a mean of 167 men, 09 mg / dL and women of 164.39 mg / dL. This study concludes that all patients with CKD have increased levels of urea and creatinine by more than 100%.
DIFFERENCES OF INHIBITION ZONE FOREST PURE HONEY AND CINNAMON (Cinnamomum burmani) AS ANTIBACTERIAL BACTERIAL COMPOUNDS OF MEMBERS OF THE Enterobacteriaceae FAMILY Eko Naning Sofyanita; Roni Afriansya
Jurnal Riset Kesehatan Vol 10, No 1 (2021): MAY 2021
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (450.538 KB) | DOI: 10.31983/jrk.v10i1.6424

Abstract

Honey and cinnamon are plant products that have many properties, one of which is used as an antibacterial agent for various diseases. The effectiveness of the two active substances against bacteria in the Enterobacteriaeae family is unclear. The study was conducted to determine the differences in the effectiveness of forest honey and cinnamon in inhibiting the growth of bacteria in the Enterobacteriaceae family. This research method is an experimental study with a combination method of diffusion and wells using samples of the bacteria Klebsiella pneumoniae, Esherichia coli, Salmonella typhi, Serratia marcescens, and Enterobacter cloacae. Forest pure honey and cinnamon were prepared as test solutions with concentrations of 25%, 40%, 55%, 70%, 85% and 100%. The results obtained were the inhibition zone formed on Nutrient Agar Plate (NAP) media, in forest pure honey the inhibition zone formed in S. typhi, E. coli, Klebsiella pneumoniae, Serratia marcescens, and Enterobacter cloacae was greater at 100% concentration, namely 30.5mm, 32.5mm, 38mm, 29.5mm, 26mm, while the 100% concentration of cinnamon showed results, namely 12mm, 10mm, 8.5mm, 11mm, and 11mm. Conclusion is Forest pure honey was more effective at inhibiting bacteria from the Enterobacteriaceae family than cinnamon.
The Effect of Light Exposure to Bilirubine Levels on Serum Jaundice Infant in Hospital of Islamic NU Demak Eko Naning Sofyanita; Endang Susilowaty; Roni Afriansya
Borneo Journal of Medical Laboratory Technology Vol 3 No 1 (2020): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v3i1.1766

Abstract

Bilirubin is a substance formed from the normal breakdown of erythrocytes in the body so that it gives a yellow color to the stool and urine. The test of bilirubin in the laboratory must avoid exposure to light, which can cause decreased serum bilirubin levels by up to 50% in 1 hour due to disruption of the bilirubin's stability. This study is a cross-sectional analytic study of primary data using 30 samples of jaundice baby serum and direct and total bilirubin test. Data collection was conducted from the primary data by a direct test using 40 samples of infant jaundice and test of direct bilirubin and total bilirubin using methods Dichlorophenyl Diazonium. The results of the tests of 40 samples can result in the mean levels of total bilirubin were exposed to the light of 8.58 mg/dl and were not exposed to light 12,67mg /dl. Direct bilirubin levels mean that exposure to light is 3.98 mg/dl. In contrast, the unexposed light of 8.71 mg/dl, so that it can be concluded that the levels of total and direct bilirubin in serum jaundice infants exposed to lower light compared with those not exposed to light.
Hubungan Jumlah Eritrosit dan Hitung Jenis Leukosit pada Masyarakat dengan Paparan Polutan Tinggi di Desa Tambak Mulyo Kota Semarang Eko Naning Sofyanita; Hendra Adistia Bangkit; Roni Afriansya
Borneo Journal of Medical Laboratory Technology Vol 3 No 2 (2021): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v3i2.2230

Abstract

Lead (Pb) and carbon monoxide (CO) are gas components in airborne pollutants due to vehicle fumes or industrial exhaust gases that affect the blood, namely shortening the life span of erythrocytes produced in erythropoiesis. In addition, small pollutants can be inhaled and enter the alveoli and then phagocytosis and trigger the release of mediators, namely leukocyte cells, which can cause inflammation. This study aims to determine the correlation between the erythrocytes count and differential leucocyte count of the Tambak Mulyo village community, Semarang City. This research was a cross-sectional analytic study of primary data using 20 blood samples, carried out by the erythrocytes count and differential leucocyte count tests. The examination results showed that the average erythrocytes count in all samples was 5.25 million cells/mm3 with a percentage of the normal erythrocytes count is 45.00%, and a high of erythrocytes count is 55.0%. On the test of the differential leucocyte count, 4 samples are obtained with specific values. Normal leukocytes and as many as 16 samples experienced an increase and decrease in the differential leucocyte count. The chi-square test shows Asymp.Sig> 0.05, which means there is no correlation between variables, so it can be concluded that there is no significant correlation between the erythrocytes count and differential leucocyte count (lymphocytes, basophils, eosinophils, neutrophils, and monocytes) in the community with exposure to high pollutants in Tambak Mulyo village, Semarang City.
Correlation Of Erythrocyte Sedimentation Rate (ESR) and Lymphocyte Counts in Pulmonary Tuberculosis Patients during Intensive Treatment in Purwokerto City Eko Naning Sofyanita
Borneo Journal of Medical Laboratory Technology Vol 4 No 1 (2021): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v4i1.2722

Abstract

Pulmonary tuberculosis is a disease caused by the bacterium Mycobacterium tuberculosis which most often attacks the lungs by spreading through the air in patients who cough, sneeze or spit. In tuberculosis, the erythrocyte sedimentation rate examination is used to help diagnose the course of the disease and help for the success of chronic therapy, and the examination of the lymphocyte count is used to support the diagnosis of bacterial infection. This study is an analytic study through a cross-sectional approach with the Pearson Correlation test. The results of this LED examination were 8 samples (21%) normal and 30 samples (79%) abnormal. On examination of the lymphocyte count, 21 samples (55%) had lymphopenia, 16 samples (42%) were normal patients, and 1 sample (3%) had lymphocytosis. In the Pearson correlation test, the correlation is unidirectional but moderately or moderately correlated (r=0.304). Based on these results, it can be concluded that there is a relationship between erythrocyte sedimentation rate and lymphocyte count. This means that if the LED value is large, the number of lymphocytes will also increase.
Minyak Zaitun Sebagai Pengganti Xylene pada Prosesing Jaringan Histologis untuk Pewarnaan Kulit dan Hepar Mencit dengan Hematoxylin Eosin: Sebuah Studi Perbandingan Eko Naning Sofyanita; Arya Iswara; Djoko Priyatno
Jaringan Laboratorium Medis Vol 4, No 2 (2022): November 2022
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v4i2.8688

Abstract

Clearing results in histological processing can be affected due to tissue density and viscosity of the clearing agent. Xylene is the most commonly used aromatic solvent for clearing agents and deparaffinizing agents in histopathology laboratories, but xylene is one of the most dangerous chemicals found in histology laboratories. Based on the dangers posed by xylene, a replacement material is needed. Several xylene substitutes such as reagents derived from limonene, aliphatic hydrocarbons, vegetable oils and mineral oils have been commercially developed, but the available xylene substitutes are still less effective, more expensive, and still as dangerous as xylene itself. Substitutes that are considered safer are from natural oils, one of which is olive oil which has similarities with xylene, namely in hydrocarbon and phenol compounds. This study used two types of tissue, namely skin and liver from mice (Mus musculus) which were cut into two parts; the first part uses xylene as and the other part uses olive oil as a clearing agent. The assessment of hematoxylin eosin staining results were determined based on the category of assessment of cell structure, such as the cell nucleus, cytoplasm, and color uniformity carried out by three readers and five fields of view for each microscopic slide at 40X magnification of the objective lens. Comparison of observations of the xylene group in skin and liver tissue 100% got a good score on the cell nucleus, cytoplasm, and color uniformity. (p=1,000). The olive oil group had a slight difference in color uniformity in liver tissue when compared to skin, but not statistically different (p=0.773). The comparison of the overall readings of the xylene and olive oil groups on skin and liver tissue also showed no statistically different (p=0.262). So it can be concluded that olive oil can be recommended as a substitute for xylene in the clearing process in histological tissue processing.
Deteksi Karsinoma Nasofaring Mengunakan Rapid Test Uji Imunokromatografi pada Petugas SPBU Roni Afriansya; Umi Rosidah; Eko Naning Sofyanita
Jaringan Laboratorium Medis Vol 5, No 1 (2023): May 2023
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v5i1.9337

Abstract

Nasopharyngeal cancer (NPC) is the 4th most common malignancy in Indonesia, after breast, cervical and lung cancer. The risk factors for this cancer are Epstein-Barr virus, gender, race, adult age 30-50 years, family history, drinking alcohol and smoking. The prevalence of NPC in Indonesia is 6.2/100,000, with nearly 13,000 new cases, but this is a small proportion that has been documented. Adham et al (2012) reported that NPC is the most common head and neck cancer (28.4%), with a male-female ratio of 2:4 and is endemic to the island of Java. The existence of knowledge about NPC starting from prevention, early detection, appropriate treatment will be able to help overcome problems caused by nasopharyngeal cancer. Early detection is very important. If NPC is found at an early stage, the cure rate is quite high, reaching 90%. The purpose of this study was to find out whether there were gas station workers in Semarang City who experienced NPC. This study used an analytic survey method with a cross-sectional approach. The results of the study of 20 Semarang City Gas Station officers found that the characteristics based on age were the most in the range 31-50, namely 70%. Characteristics based on gender of the 20 gas station attendants are all male (100%). Characteristics based on length of service are 1 - 10 years (35%), 11 - 20 years (35%) and 21 - 30 years (30%) respectively. Characteristics based on smoking history, 55% of respondents smoked and 45% did not smoke. And the NPC examination results of all respondents (100%) were NPC negative.
Co-Authors Achmad Zulfa Juniarto Aniesa Izzatun Nafsi Anna Sufi Annisa Anung Sugihartono Ari Suwondo Arya Iswara Auliya, Qurrotu A'yuni Burhannudin Dina Afrianti1 Djoko Priyatno Duri, Iin Demiany Elis Susilawati Endang Susilowaty Errina Septiyasari Faiz Shofa Abdillah Hadipranoto, Ichsan Hendra Adistia Bangkit Heri Nugroho I Made Wisnu Darma Putra Kurniasiwi, Putri Lilik Setyowatiningsih Maulana, Mochamad Rizal Nabila Azahra Nabila, Jihan Nanik Aryani Putri Nazhiifah, Tristania Shofi Nur Indah Palupi NURUL QOMARIYAH Nurul Qomariyah PUJI LESTARI Qurrotu A’yuni Auliya Rihanesa Diana Putri Ririh Jatmi Wikandari Roni Afriansya S.Y. Didik Widiyanto Setyowatiningsih, Lilik Siti Thomas Zulaikhah Suwarsi Suwarsi Swastya Putri, Adita Puspitasari Swastyaputri, Adita Puspitasari Syanubari, Sonnia Pusparindah Taufiqurrachman Nasihun Umi Rosidah Utami Purnama Siwi