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All Journal Jurnal Ilmu Pertanian Indonesia Microbiology Indonesia Jurnal Penelitian Pascapanen Pertanian
Liesbetini Hartoto
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology

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Peran PEG 400 dalam pembuatan lembaran bioplastik polohidroksialkanoat yang dihasilkan oleh Ralstonia eutropha dari Substrat Hidrolisat Pati Sagu Khaswar Syamsu; Liesbetini Hartoto; Anas Miftah Fauzi; Ani Suryani; Dede Rais
Jurnal Ilmu Pertanian Indonesia Vol. 12 No. 2 (2007): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (164.904 KB)

Abstract

The purpose of the research was to investigate the effects of PEG 400 addition on the characteristics of bioplastic polyhydroxyalkanoates (PHA). PHA was obtained by cultivating Ralstonia eutropha on hydrolysed sago starch substrate using fed batch method for approximately 96 hours. The biomass concentration obtained was 4 g/L with PHA yield 20-30% of dry cell weight. The bioplastic was formed with solution casting method in which chloroform was used as solvent and PEG 400 was used as plasticizer. The concentrations of PEG 400added were 10, 20, and 30% (w/w), respectively. Bioplastic properties which were tested were tensile strength, elongation to break, density, thermal properties, cristalinity, and functional group. The addition of plastisizer tend to increase tensile strength and elongation to break, but decrease density, cristalinity and melting point. Bioplastic with 30% PEG 400 addition gave the best results. Bioplastic with 30% PEG 400 gave a value of tensile strength of 0.083 MPa; elongation to break of 0.881%; density of 0.7881 g/cm3;  melting point of 158.95 ac; and cristalinity of 44.58%. With these properties, the resulted bioi plastic may be used for surgical strings. Keywords: Bioplastic, Polyhydroxyalkanoates (PHA), Ralstonia eutropha, hidrolysed sago starch, PEG 400
Designing Packed Palm Cooking Oil Distribution at Traditional Market in Jakarta Using Fuzzy Clustering Teja Primawati Utami; Syamsul Ma’arif; Yandra Arkeman; Liesbetini Hartoto
Jurnal Penelitian Pascapanen Pertanian Vol 12, No 3 (2015): Jurnal Penelitian Pascapanen Pertanian
Publisher : Balai Besar Penelitian dan Pengembangan Pascapanen Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jpasca.v12n3.2015.105-114

Abstract

Referring to the Indonesian National Standard number 7709 year 2012 that palm cooking oil should be fortified with vitamin A, so that the distribution process required packaging to protect Vitamin A. Besides packaging palm cooking oil can make the content hygiene. Packaged cooking oil was distributed from the factory to the traditional market directly. In accordance with Regulation of the Minister of Industry of the Republic of Indonesia Number 87 year 2013 on the application of ISO palm olein is mandatory and Trade Minister Regulation number 80 year 2013 on compulsory packaging cooking oil, so need a mechanism to bulk cooking oil distribution.Simple packaging in the traditional market of producers to effective and efficient consumer. The purpose of this paper is to design a system of distribution of cooking oil from producers to consumers in traditional markets by creating a central cluster calls distribution center. Design models created using fuzzy clustering method. The results of this study create 15 clusters of traditional markets in Jakarta.Merujuk pada Standar Nasional Indonesia nomor 7709 tahun 2012 bahwa minyak goreng sawit perlu ditambahkan vitamin A, sehingga dalam proses distribusinya diperlukan pengemasan untuk melindungi Vitamin A tersebut. Tujuan pengemasan minyak goreng curah ke kemasan sederhana juga dilakukan untuk menjaga higienitas dan melindungi konsumen dari praktek pengoplosan. Sesuai dengan Peraturan Menteri Perindustrian Republik Indonesia nomor 87 tahun 2013 tentang pemberlakuan SNI minyak goreng sawit secara wajib dan Peraturan Menteri Perdagangan nomor 80 tahun 2013 tentang minyak goreng wajib kemasan, maka diperlukan suatu mekanis pendisribusian minyak goreng curah ke kemasan sederhana di pasar tradisional dari produsen ke konsumen yang efektif dan efisien. Tujuan dari makalah ini adalah mendesain sistem distribusi minyak goreng dari produsen ke konsumen di pasar tradisional dengan cara membuat klaster yang otomatis ditentukan sentral distribusinya. Desain model dibuat dengan menggunakan metode Fuzzy clustering. Hasil dari penelitian ini adalah terdapat 15 klaster pasar tradisional di Jakarta dengan masing-masing satu sentra distribusi.
Isolation and Characterization of Antimicrobial Substance from Marine Streptomyces sp. ROFIQ SUNARYANTO; BAMBANG MARWOTO; TUN TEDJA IRAWADI; ZAINAL ALIM MAS'UD; LIESBETINI HARTOTO
Microbiology Indonesia Vol. 4 No. 2 (2010): August 2010
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (6996.369 KB) | DOI: 10.5454/mi.4.2.7

Abstract

Isolation and purification of antimicrobial active substance produced by marine Actinomycetes has been carried out. Marine sediment samples were obtained from six different places at Banten West Coast. Isolation was conducted using two pretreatment methods, acid and heat shock pre-treatment. A total of 29 Actinomycetes isolates were obtained from the various sediment samples collected, then tested for antimicrobial activity against Escherichia coli ATCC 25922, Staphylococcus aureus ATCC25923, Pseudomonas aeruginosa ATCC27853, Bacillus subtilis ATCC 66923, Candida albicans BIOMCC00122, and Aspergillus niger BIOMCC00134. Among the isolates, isolate A11 was the most activity to Gram-positive and Gram-negative bacteria, and morphological observation and identification using 16S rRNA showed that the isolate was similar to Streptomyces sp. Production of active compound from A11 isolate used yeast peptone medium. Purification of active compounds was carried out using silica-gel-column chromatography and preparative HPLC. A single peak of active compounds was detected by HPLC, which showed a retention time of 8.35 min and maximum absorbance in UV visible at 210 nm and 274.5 nm respectively.
Co-Authors Anas Miftah Fauzi Ani Suryani BAMBANG MARWOTO Dede Rais Khaswar Syamsu Rofiq Sunaryanto Syamsul Ma’arif Teja Primawati Utami Tun Tedja Irawadi Yandra Arkeman Zainal Alim Mas'ud