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STABILITAS EDIBLE FILM PATI SAGU (METROXYLON SAGU ROTTB.) ASETAT SELAMA PENYIMPANAN PADA BERBAGAI SUHU Rosniyati Suwarda; Tun Tedja Irawadi; Prayoga Suryadarma; Indah Yuliasih
Jurnal Teknologi Industri Pertanian Vol. 29 No. 3 (2019): Jurnal Teknologi Industri Pertanian
Publisher : Department of Agroindustrial Technology, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24961/j.tek.ind.pert.2019.29.3.278

The degree of stability of starch-based edible films during storage varies greatly compared to synthetic films. This work aimed to study the effect of storage temperatures on the stability of sago starch acetate edible film during storage. Edible films produced by casting and storage at 5, 30 and 40 oC temperatures for 34 days. The observed characteristics were crystallinity, contact angle, physical, mechanical and barrier (WVTR) film properties. Sago starch acetate edible films presented lower relative crystallinity (10,10%) and highest contact angle (51,04o) when compared with native starch edible films. The results indicated that sago starch acetate edible films were relatively stable when stored at low (5 oC) and high (30 and 40 oC) temperatures. Storage temperatures did not affect physical properties (solubility, transparency) and mechanical (elongation at break / EAB) of sago starch acetate edible films except tensile strength (TS) and water vapor transmission rate (WVTR). TS values increased at temperatures 30 and 40 oC and WVTR values increased at temperature 30 oC. Changes in physical and mechanical properties occur at the beginning of storage, but then tend to be stable until the end of storage. Sago starch acetate edible films had low physical and mechanical characteristics, that was TS (2.25–4.90 MPa), elongation at break (29.65–89.62%), solubility (15.01–29.61%), transparency (0.91–1.87) and very low WVTR value (0.07–0.33 g/m2.hour) and Ea (-8.6E-04 kJ/mol). Sago starch acetate edible films had good physical and mechanical properties and resistant to moisture, can be used for applications as edible films and coatings for foods that contain high water content. Keywords: edible film, mechanical and physical properties, sago starch acetate, water vapor transmission rate

Penentuan Lama Sulfonasi pada Proses Produksi Surfaktan Mes untuk Aplikasi EOR Mira Rival; Tun Tedja Irawadi; Ani Suryani; Dwi Setyaningsih; Erliza Hambali
Jurnal Ilmu Pertanian Indonesia Vol. 16 No. 1 (2011): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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For producing oil remains that remained at old oil wells (mature field), a method of advanced oil acquirement improvement known as an Enhanced Oil Recovery (EOR) should be applied. Surfactant plays an important role in EOR process by reducing interfacial tension (1FT), altering wettability, reducing oil viscosity, and stabilizing dispersion to facilitate the process of oil jetting from reservoir to production well. To optimally cleanse oil that still remained a surfactant compatible with formation water and reservoir is needed. This study was conducted to get the best time of sulfonation process for producing MES surfactant with lower interfacialtension for EOR application. Results showed that the best times of sulfonation process with lower interfacial tension value were 3 and 4 hours.

SINTESIS PATI JAGUNG TERFOSFORILASI MELALUI TEKNIK GELOMBANG MIKRO [Microwave-Assisted Synthesis of Phosphorylated Corn Starch] Atep Dian Supardan; Suminar Setiati Achmadi; Tun Tedja Irawadi
Jurnal Teknologi dan Industri Pangan Vol. 25 No. 1 (2014): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

SINTESIS PATI JAGUNG TERFOSFORILASI MELALUI TEKNIK GELOMBANG MIKRO [Microwave-Assisted Synthesis of Phosphorylated Corn Starch] Atep Dian Supardan*, Suminar Setiati Achmadi dan Tun Tedja Irawadi Departemen Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Institut Pertanian Bogor, Bogor Diterima 28 Maret 2013 / Disetujui 24 Februari 2014ABSTRACT Phosphorylated starch is a type of modified starches which is mostly imported. Commonly, starch to be modified must contain more than 25% of amylose. This study aimed to synthesize phosphorylated starch and evaluate its potency as a heavy metal adsorbent. Corn starch was subjected to phosphorylation through microwave-assisted reaction with a mixture of sodium dihydrogen orthophosphate and disodium hydrogen phosphate. The experiment was designed to optimize the pH, microwave radiation power, and phosphorylation time. The results showed that the maximum phosphate subtitution degree was obtained at pH of 6, microwave radiation of 500 W, and a reaction time of 10 minutes. The degree of subtitution ranged from 0.567 to 0.787. The physicochemical properties of the product i.e. swelling capacity, solubility, water binding capacity, and paste clarity were significantly different than that of the unmodified corn starch. The infrared spectrum showed a high peak absorption at the wavelength of 1651 cm-1, indicating hydrogen bond formation of phosphoric group-water- phosphoric group. In the fingerprint area, there were two new absorption peaks at 1200 and 990 cm-1, which were assigned for the P=O and C-O-P vibrations, respectively. The phosphorylated corn starch adsorbed methylene blue up to 73.3% and mercury up to 73.6%, suggesting the prospect of the microwave-assisted synthetic phosphorylated corn starch as an effective adsorbent for heavy metals.

The purpose of this research is to study the effect of pyrolysis temperature to the wood vinegar chemical composition by be produced wood vinegar, charcoal, biofuel etc. The variation of pyrolysis temperatures were 110, 200, 300, 400 and 500 °C at 5 hours. That pine wood vinegar yield obtained were 13.80%, 16.12%, 11.99%, 15.51% and 0.90 %. Analysis DTA (Diferential Thermal Analysis) showed thermal decompotition occurs at 227, 320.2 and 349.7 °C. Identification of GC-MS of pine sawdust c Mohammad WIJAYA; Erliza NOOR; Tun Tedja IRAWADI; Gustan PARI
Jurnal Ilmu dan Teknologi Hasil Hutan Vol. 1 No. 2 (2008): Jurnal Ilmu Teknologi Hasil Hutan
Publisher : Departemen Hasil Hutan, IPB

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The purpose of this research is to study the effect of pyrolysis temperature to the wood vinegar chemical composition by be produced wood vinegar, charcoal, biofuel etc. The variation of pyrolysis temperatures were 110, 200, 300, 400 and 500 °C at 5 hours. That pine wood vinegar yield obtained were 13.80%, 16.12%, 11.99%, 15.51% and 0.90 %. Analysis DTA (Diferential Thermal Analysis) showed thermal decompotition occurs at 227, 320.2 and 349.7 °C. Identification of GC-MS of pine sawdust could provide compounds that mostly derived from acid group and was dominated by acetic acid produced was 31.65%. At distillate of teak smokes was obtained three yield of liquid smokes resulted from conversion of pine wood wastes can be utilized to preserve fishes. Keywords : Pine sawdust, pyrolysis, wood vinegar, and preserve fishes

Pemisahan Kardanol Dari Minyak Kulit Biji Mete Dengan Metode Destilasi Vakum nFN Risfaheri; Tun Tedja Irawadi; M. Anwar Nur; Illah Sailah; Zainal Alim Mas'ud; Meika S. Rusli
Jurnal Penelitian Pascapanen Pertanian Vol 1, No 1 (2004): Jurnal Penelitian Pascapanen Pertanian
Publisher : Balai Besar Penelitian dan Pengembangan Pascapanen Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jpasca.v1n1.2004.1-11

Minyak kulit biji mete (Cashew nut shellliquidlCNSL) merupakan hasil samping dari pengo laban kacang mete, mengandung senyawa fenolik terutama kardanol. Kardanol merupakan senyawa monohidroksi fenolik yang mempunyai rantai panjang hidrokarbon pada posisi metanya. Kardanol memiliki potensi sebagai pengganti fenol pada berbagai produk industri kimia berbasis resin fenolik. Penelitian bertujuan untuk mendapatkan kondisi optimum pemisahan kardanol dari CNSL dan mengidentifikasi karakteristik kardanol serta estimasi kelayakan produksi kardanol. Tahapan penelitian meliputi: (I) analisis sifat fisika dan kimia CNSL; (2) optimasi dekarboksilasi CNSL untuk mengkonversi asam anakardat menjadi kardanol; (3) optimasi suhu destilasi CNSL untuk pemisahan kardanol; (4) identifikasi destilat dengan GC-MS (Gas Chromatography-Mass Spectrometry), HPLC (High Performance Liquid Chromatography) dan FTIR (Fourier Transform Infrared Spectroscopy); serta (5) estimasi kelayakan produksi kardanol. Kondisi optimum dekarboksilasi dicapai dengan pemanasan 140"(: selama I jam. Kardanol dapat dipisahkan dari CNSL dengan dettilasi vakum (4-8 mmHg), dan suhu optimum dicapai pada 280"(: dengan rendemen 74,22%. Karakteristik destilat CNSL sesuai dengan spesifikasi kardanol teknis. Komponen destilat terdiri atas: 3-[8(Z),II(Z),14-pentadecatrienyl] phenol 74,25%, 3-[8(Z), II (Z),14-pentadecadienyl] phenol 10,94%, dan 3-[8(Z),11 (Z),14-pentadecieny/] phenol 14,81%. Industri produksi kardanol layak didirikan, dengan nilai NPV = Rp 5.311.121.638, IRR = 45,79%, Net B/C = 2,46 dan PBP = 2,22 tahun. Isolation of cardanol from the cashew nut shell liquid using vacuum distillation methodThe cashew nut shell liquid (CNSL) is a by product obtained during the cashew nut processing contained the phenolic constituents mainly cardanol. Cardanol is a mono hydroxyl phenol having a long hydrocarbon chain in the meta position. It has a potential as a subtitute for phenol in resin phenolic-base chemical products. The objective of the research was to find out the optimum condition in isolating the cardanol from CNSL and to identify the characteristic of cardanol and to estimate the feasibility of cardanol production. The research was carried out in several stages as followed: (I) analyses of physico-chemistry of CNSL, (2) dicarboxylic optimation of CNSL to convert anacardic acid into cardanol, (3) temperature optimation of CNSL distillation process, (4) identification of CNSL distillate using GC-MS, HPLC, and FTIR; and (5) estimation of feasibility of cardanol production. The optimal condition of the decarboxylation was heating at 14O"C for I hour. The cardanol was obtained from CNSL by vacuum distillation process at 4-8 mmHg, and the optimal temperature was achieved at 280"(: with the 74.22 % yield. The characteristics of CNSL distillate met the specification of technical cardanol. The constituent of distillate were as follow: 3-[8(Z),11 (Z),14-pentadecatrienyl]phenol 74.25 %, 3-[8(Z),11 (Z),14-pentadecadienyl]phenol 10.94 %, and 3-[8(Z),ll(Z),14-pentadecienyl]phenol 14.81%. The cardanol production industries was feasible to be implemented with NPV = Rp. 5.311.121.638, IRR = 45.79%, Net B/C = 2.46 and PBP = 2.22 years.

Seleksi dan Formulasi Media Pertumbuhan Bakteri Penghasil Xilanase Nur Richana; Tun Tedja Irawadi; Anwar Nur; Illah Sailah; Khaswar Syamsu
Jurnal Penelitian Pascapanen Pertanian Vol 3, No 1 (2006): Jurnal Penelitian Pascapanen Pertanian
Publisher : Balai Besar Penelitian dan Pengembangan Pascapanen Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jpasca.v3n1.2006.41-49

Seleksi bakreri penghasil xilanase dan formulasi media pertumbuhan bakteri penghasil xilanase telah dilakukan di laboratorium Bioproses, Balai Besar Penelitian dan Pengembangan Pascapanen Pcrtanian. Seleksi isolat bakteri dilakukan terhadap lima isolat penghasil xilanase yaitu RXAI-5, RXAII-5, RXAIII-1, RXAIII-5 dan RXNI-3), dengan membandingkan hasil kultivasi meliputi biomasa, protein terlarut, akrivitas xilanase dan aktivitas spesifik. Formulasi media dilakukan dengan mengoptimasi konsentrasi peptone, ekstrak khamir sebagai sumbcr nitrogen dan oat spelt xylane sebagai sumber karbon. Analisis percobaan menggunakan rancangan acak faktorial, faktor (A) peptone terdiri atas empat taraf yaitu A1=0, A2=0,I; A3=0,3; A4=0,5% Faktor (B) ekstrak khamir terdiri alas tiga taraf (B1=0,1; B2=0,2; B3=0,3%) dan faktor (C) oat spelt xylene terdiri atas tiga taraf (C1=0,5; C2=0,75; C3=1,0%) dengan tiga kali ulangan. Hasil penelitian menunjukkan bahwa isolat baktcri Bacillus pumilus RXAIII-5 dinyatakan sebagai isolat bakteri unggul diantara kelima isolat bakteri penghasil xilanase. Pada formulasi media ternyata protein terlarut tertinggi (0,596 g/l) pada media dengan komposisi 0,75% xilan, pepton 0,5%, ekstrak khamir 0.2%. Aktiviras xilanase dan aktivitas spesifik tertinggi berturut-turut adalah 186,37 u/ml dan 436,45 U/mg protein. Keduanya dicapai pada komposisi media yang sama yaitu pepton 0, 1 %, ekstrak khamir 0, I %, dan xilan 0,5%. Dcngan demikian komposisi tersebut merupakun komposisi mcdi a terpilih yang optimum. Isolat bakteri unggul bersifat alkali ini diharapkan dapar menghasilkan xilanase yang tahan pada pH tinggi sehingga dapat digunakan untuk proses pemutihan kertas yang ramah lingkungan. Selection and Growth Medium-Formulation of Xylanase Producing BacteriumThis research was carried out in the bioprocess laboratory of Indonesian Center for Agricultural Postharvest Research and Development, Bogor. Selection of five isolates (RXAI-5, RXAII-5. RXAIII-1, RXAIII-5 and RXNI-3). xylanase producing bacteria Were based on comparative study of cultivation yield consists of biomass of bacterium cells, dissolved protein, xylanase activity and specific activity, Formulation of growth medium using peptone and yeast extract as nitrogen source and oat spclt xylan as carbon source. Design experiment used at formulation of growth medium was randomized factorial design. with factor A) peptone consist of four level, A 1=0. A2=0.1; A3=0.3; A4=0.5%, factor (8) yeast extract consist or third level (131=0.1, 132=0.2; 133=0.3%) and factor (C) oat spelt xylan consist of three level (CI=0.5; C2=0.7S; C3=1.0%), with three replication. Research result showed that Bacillus pumilus RXAIII-5 is the best bacterium isolate among five isolates of xylanase producing bacteria. In growth medium formulation showed that highest dissolved protein (0.596 g/l) was achieved in the medium containing 0.75% xylan, 0.5% pepton, and 0.2% yeast extract. The highest value of both of xylanase activity and specific activity are 186.37 u/ml and 436.45 U/ml respectivelly. In fact these were reached at similar growth medium composition of 0.1 % pepton, 0.1 % yeast extract, and 0.5% xylan, and consequently became the best of growth media formulation. The potential alcaliphilic bacterial isolate is expected to produce xylanase with high pH stability. The enzyme can be used as environmentaly safe agent for paper bleaching.

Ekstraksi Xilan dari Tongkol Jagung Nur Richana; Tun Tedja Irawadi; M. Anwar Nur; Illah Sailah; Khaswar Syamsu; Yandra Arkenan
Jurnal Penelitian Pascapanen Pertanian Vol 4, No 1 (2007): Jurnal Penelitian Pascapanen Pertanian
Publisher : Balai Besar Penelitian dan Pengembangan Pascapanen Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jpasca.v4n1.2007.38-43

Tongkol jagung merupakan limbah jagung terbesar (45%) yang mengandung xilan sekitar 12% dan belum banyak dimanfaatkan. Tongkol jagung mempunyai prospek untuk bahan baku industri xilan, yang merupakan bahan baku industri furfural, gula xilitol, dan bahan baku untuk media pertumbuhan bakteri xilanase. Penelitian ini bertujuan untuk mendapatkan metode ekatraksi xilan dari tongkol jagung dan karakterisasi xilan yang dihasilkan. Ekstraksi xilan dari tongkol jagung dilakukan dengan dua tahap yaitu proses delignifikasi menggunakan NaOCl (0,5; 1,0; 2,5; 5 dan 7,5%) kemudian pengendapan xilan dengan perlakuan rasio supernatan dan etanol 1:1; 1:2; 1:3; dan 1:4. Ekstrak xilan selanjutnya diuji kelarutan pada pelarut organik, asam, alkali, air panas dan dingin. Analisis kualitatif dan kuantitatif xilan dilakukan dengan Khromatografi Cair Kinerja Tinggi . Hasi l penelit ian menunjukkan kombinasi perlakuan konsentrasi NaOCl 0,5% dan perbandingan supernatan:etanol 1:3 (v/v), menghasilkan rendemen xilan tertinggi (12,95%). Analisis dengan Khromatografi Cair Kinerja Tinggi menghasilkan khromatogram xilan dengan kemurnian 97,47%. Xilan yang dihasilkan sangat larut dalam alkali (NaOH 1%), larut dalam air panas dan dingin.Extraction Of Xylan From Corn CobCorn cob is the biggest part of corn waste (45%) which contain about 12% xylan which has not been utilized. Corn cob can be used as raw material of production of xylan. Xylan is the raw material for furfural, xylitol industry, and growth medium for microorganism producing xylanase.The objectives of this research was to find out the method of extracting the xylan, and characterization of xylan produced. The extraction procedure were done in two steps, firstly delignification using NaOCI (0.5; 1.0; 2.5; 5 and 7.5%) and then precipitation using ethanol with the ratio of ethanol and supernatant (I: I; 1:2; 1:3; and 1:4). The solubility of xylan produced in organicsolvent, acid, alkaline solvent and in cold and hot water was observed. Quantitative and qualitative analysis of xylan were done using High Performance Liquid Chromatography. The research result showed that the highest yield (12.95%) whith purity (97.47%) was produced by 0.5% NaOCI and 1 : 3 ratio of ethanol and supernathant combination treatment. The highest solubility of xylan was in alkaline solvent (NaOH 1%), and soluble in hot and cool water.

Optimasi Komposisi Kardanol Dari Minyak Kulit Mete Sebagai Subtitusi Fenol Dalam Formulasi Perekat Fenol Formaldehida nFN Risfaheri; Tun Tedja Irawadi; M. Anwar Nur; IIlah Sailah; Zainal Alim Mas'ud; Meika S. Rusli
Jurnal Penelitian Pascapanen Pertanian Vol 2, No 1 (2005): Jurnal Penelitian Pascapanen Pertanian
Publisher : Balai Besar Penelitian dan Pengembangan Pascapanen Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jpasca.v2n1.2005.24-33

Minyak kulit biji mete merupakan hasil samping dari pengolahan kacang mete, mengandung senyawa fenolik terutama kardanol. Penelitian ini bertujuan mendapatkan komposisi kardanol yang optimum sebagai substitusi fenol dalam formulasi perekat fenol formaldehida. Pelaksanaan penelitian dibagi atas beberapa tahap, yaitu (l) optimasi komposisi mol senyawa fenolik, nisbah mol senyawa fenolik terhadap formaldehida, dan lama reaksi (2) optimasi komposisi mol senyawa fenolik dan pH reaksi, dan (3) analisis struktur resin perekat dengan FTIR (Fourier Transform infrared Spectroscopy). Formulasi perekat yang optimum diperoleh dengan komposisi senyawa fenolik (I mol kardanol : I mol fenol), nisbah mol formaldehida terhadap senyawa fenolik (1,5 : 1,0). Kondisi optimum pembuatan perekat dicapai pada reaksi polikondensasi pH 10 dan berlangsung selama I jam. Kardanol dapat menggantikan fenol sebanyak 70 % dalam fonnulasi perekat fenol formaldehida. Perekat tersebut menghasilkan keteguhan rekat kayu lapis dalam keadaan kering dan basah (setelah direbus selama 72 jam) rata-rata 15,36 kg/em? dan 13,61 kg/em'. Persyaratan keteguhan rekat untuk perekat fenol formaldehida menurut Standar Nasional Indonesia 06-4567-1998, yaitu minimum 10 kg/em! (hasil uji dalam keadaan kering) dan 8 kg/em' (hasil uji dalam keadaan basah). Terjadi sinergis antara kardanol dan fenol pada reaksinya dengan formaldehida, sehingga reaksi formaldehida dengan kardanol tidak hanya pada cincin aromatiknya tetapi juga terjadi pad a rantai samping tidak jenuh (CIS) dari kardanol, sehingga meningkatkan keteguhan rekat kayu lapis. Optimation of cardanol composition from. cashew-nut shell liquid as phenol subtitute in phenol formaldehyde adhesives formulationThe cashew nut shell liquid is a by product obtained from the cashew nut processing, contains phenolics compound mainly cardanol. The objective of the research was to find out the optimum cardanol composition as phenol subtitute in phenol formaldehyde adhesives formulation. The experiment was carried out in several stages as followed: (I) optimation of mole composition of the phenolics and mole ratio of the phenolics to formaldehyde, and reaction time; (2) optimation of mole composition of the phenolics and pH reaction; and (3) the resin structure analysis using FTIR (Fourier Transform Infrared Spectroscopy). The optimum formulation of adhesive synthesis as followed: the composition of phenol portion (I mole cardanol : I mole phenol), the mole ratio of formaldehyde to total phenol (1.5 : 1,0). The optimum condition of polycondensation reaction was achieved at pH 10 for I hour. Cardanol could substitute phenol as much as 70 % in the fenol formaldehyde adhesives formulation. The average of the bond strength of plywood produced using the adhesives in dry and wet condition (after boiled 72 hours) was 15.36 kg/em? and 13.61 kg/em! respectively. Requirement of the bond strength in Indonesian National Standard 06-4567-1998 is minimum 10 kg/em? (the result test on dry condition) and 8 kg/em- (the result test on wet condition). There are synergism of cardanol and phenol in reaction with formaldehyde, so therefore reaction of formaldehyde and cardanol did not only happen at the aromatics ring but also at unsaturated chain CIS of cardanol which caused an increase in the bond strength of plywood.

The Process of Xylanase Production from Bacillus pumilus RXAIII-5 NUR RICHANA; TUN TEDJA IRAWADI; M. ANWAR NUR; ILLAH SAILAH; KHASWAR SYAMSU
Microbiology Indonesia Vol. 1 No. 2 (2007): August 2007
Publisher : Indonesian Society for microbiology

The optimum conditions for the growth of Bacillus pumilus RXAIII-5 (a potential xylanase producer) were sought, these included temperature, pH, aeration, and agitation of the culture batch. Afterwards a mathematical model based on the parameter of cultivation kinetics was formulated. At the same time, the rheology of the fluid used for bacterial cultivation in a bioreactor was studied. The data obtained was used for estimating the ‘scaling up’ of enzyme production. The results of the study indicate that the optimum condition for processing in 50 ml Erlenmeyer flask are used temperature of 35 oC (308oK), pH 7, and an agitation rate of 140 rpm. The highest xylanase activity and its specific activity are 297.132 U.ml-1 and 655.32 U.g-1protein, respectively. Subsequent experiments in a bioreactor using all of the experiment parameters mentioned above, except for the agitation rate, shows that the results are as follows. The highest specific growth was at 0.082 hour-1 at an aeration and agitation rate of 0.5 vvm and 150 rpm, respectively. Based on the data of the cultivation kinetics, the optimum conditions for the fermentation in Biostat 2L-bioreactor is 1 vvm and 200 rpm of aeration and agitation, respectively . The efficiency of substrate (Yp/s) and of cell biomass (Yp/x) to produce xylanase is 50.744 U.g-1 and 43.906 U.g-1, respectively. The efficiency of substrate to cell production (Yx/s) is 1.178g.g-1. The liquid cultivation-medium has non-Newtonian properties. Based on a mathematical model it is found that the consistency index (k constant) and index of liquid behavior (n value) are 0.179 g.cm-1.second-1 and 0.3212, respectively. Becouse the value of 0

Isolation and Characterization of Antimicrobial Substance from Marine Streptomyces sp. ROFIQ SUNARYANTO; BAMBANG MARWOTO; TUN TEDJA IRAWADI; ZAINAL ALIM MAS'UD; LIESBETINI HARTOTO
Microbiology Indonesia Vol. 4 No. 2 (2010): August 2010
Publisher : Indonesian Society for microbiology

Isolation and purification of antimicrobial active substance produced by marine Actinomycetes has been carried out. Marine sediment samples were obtained from six different places at Banten West Coast. Isolation was conducted using two pretreatment methods, acid and heat shock pre-treatment. A total of 29 Actinomycetes isolates were obtained from the various sediment samples collected, then tested for antimicrobial activity against Escherichia coli ATCC 25922, Staphylococcus aureus ATCC25923, Pseudomonas aeruginosa ATCC27853, Bacillus subtilis ATCC 66923, Candida albicans BIOMCC00122, and Aspergillus niger BIOMCC00134. Among the isolates, isolate A11 was the most activity to Gram-positive and Gram-negative bacteria, and morphological observation and identification using 16S rRNA showed that the isolate was similar to Streptomyces sp. Production of active compound from A11 isolate used yeast peptone medium. Purification of active compounds was carried out using silica-gel-column chromatography and preparative HPLC. A single peak of active compounds was detected by HPLC, which showed a retention time of 8.35 min and maximum absorbance in UV visible at 210 nm and 274.5 nm respectively.

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