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All Journal Microbiology Indonesia Jurnal Kimia Terapan Indonesia Seminar Nasional Lahan Suboptimal
Rofiq Sunaryanto
Center of Biotechnology, Puspiptek, Serpong, Tangerang Selatan, Banten 15340, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Other

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Isolation and Characterization of Antimicrobial Substance from Marine Streptomyces sp. ROFIQ SUNARYANTO; BAMBANG MARWOTO; TUN TEDJA IRAWADI; ZAINAL ALIM MAS'UD; LIESBETINI HARTOTO
Microbiology Indonesia Vol. 4 No. 2 (2010): August 2010
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (6996.369 KB) | DOI: 10.5454/mi.4.2.7

Abstract

Isolation and purification of antimicrobial active substance produced by marine Actinomycetes has been carried out. Marine sediment samples were obtained from six different places at Banten West Coast. Isolation was conducted using two pretreatment methods, acid and heat shock pre-treatment. A total of 29 Actinomycetes isolates were obtained from the various sediment samples collected, then tested for antimicrobial activity against Escherichia coli ATCC 25922, Staphylococcus aureus ATCC25923, Pseudomonas aeruginosa ATCC27853, Bacillus subtilis ATCC 66923, Candida albicans BIOMCC00122, and Aspergillus niger BIOMCC00134. Among the isolates, isolate A11 was the most activity to Gram-positive and Gram-negative bacteria, and morphological observation and identification using 16S rRNA showed that the isolate was similar to Streptomyces sp. Production of active compound from A11 isolate used yeast peptone medium. Purification of active compounds was carried out using silica-gel-column chromatography and preparative HPLC. A single peak of active compounds was detected by HPLC, which showed a retention time of 8.35 min and maximum absorbance in UV visible at 210 nm and 274.5 nm respectively.
Optimasi Media Kultivasi Senyawa Aktif Penicillium lagena sebagai Antifungi Patogen Phellinus lamaoensis dengan Menggunakan Respon Surface Methodology Rofiq Sunaryanto; Diana Nurani; Asep Riswoko; Siti Nabilah; Khaswar Syamsu
Seminar Nasional Lahan Suboptimal 2018: Prosiding Seminar Nasional Lahan Suboptimal "Tantangan dan Solusi Pengembangan PAJALE dan Kela
Publisher : Pusat Unggulan Riset Pengembangan Lahan Suboptimal (PUR-PLSO) Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (589.616 KB)

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Sunaryanto et al, 2019. Optimization of Penicillium lagena Medium Cultivation on Antifungal Pathogen of Phellinus lamaoensis using Response Surface Methodology. pp. 410-420. Phellinus lamaoensis (Murr.) Hein is fungal pathogen that can cause brown root rot disease in cocoa, tea, rubber, and coffee plants. Endophytic fungi, Penicillium lagena, isolated from bandotan (Ageratum conyzoides Linn.), medicinal plant, is able to inhibit the growth of pathogenic, P. lamaoensis. The effect of carbon source, nitrogen source, and mineral solution was studied. Lactose, yeast extract, and mineral solution were media components which showed significant effect toward production of P. lagena active compound. Composition optimization of these three medium components was done by response surface methodology (RSM). The Optimal response region of the significant factor was predicted by using a second order polynomial model with statistical design, central composite design (CCD). Higest production of P. lagena active compound by quadratic model was predicted to be 69.233% with medium composition 44.77 g L-1 lactose, 13.02 g L-1 yeast extract, and 15.95 mL L-1 mineral solution. Verification value in laboratory is 58.365%, lower 15.7% than its prediction. Optimization increase P. lagena active compound 9 fold compared to unoptimize media.
SINTESIS POLI (GLIKOLIDA-CO-LAKTIDA) DAN UJI BIODEGRADABILITASNYA Rofiq Sunaryanto; Hardaning Pranamuda
Jurnal Kimia Terapan Indonesia Vol 17, No 2 (2015)
Publisher : Research Center for Chemistry - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (546.527 KB) | DOI: 10.14203/jkti.v17i2.27

Abstract

Polylactide and polyglycolide are important aliphatic polyester consist of lactide and glycolide repeats unit. It is considered as green polymer due to it is produced from renewable agricultural resources. The development of biodegradable plastics based on agricultural resources is still important to be done. Copolymerization of glycolide with D-lactide, L-lactide, and DL-lactide using a Sn(Oct)2 as catalyst had been carried out. Polymerization was conducted by ring-opening of lactide and glycolide without the solvents but melting polymerization. Polymerization were conducted at 130 °C for 130 hours. Result of this study showed that polymerization of glycolide with D-lactide produce polymers with an average molecular weight of 8.2x103 and a yield of 72 %. Polymerization of glycolide with L-lactide produce polymer with an average molecular weight of 2.8x103 and a yield of 36 %. Polymerization glycolide with DL-lactide produce polymer with an average molecular weight of 2.5x103 with a 48 % yield. The burial test for 28 days showed poly (glycolide-co-Llaktida) degraded faster than other polymers, namely degraded 32 %. While poly (glycolide-co-DL-lactide) and poly (glycolide-co-D-lactide) showed similar degradation that is equal to 20 %. Compared with PHB (polyhydroxybutyrate), poly (glycolide-co-lactide) degraded more slowly.Keywords: Glycolide, lactide, copolymerization, biodegradable plastic
Co-Authors ASEP RISWOKO BAMBANG MARWOTO Diana Nurani Hardaning Pranamuda Khaswar Syamsu Liesbetini Hartoto Siti Nabilah Tun Tedja Irawadi Zainal Alim Mas'ud