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All Journal Indian Journal of Forensic Medicine & Toxicology
Essam F. A. Al-Jumaili
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Public Health

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Purification and Characterization of Arginase from Serum of Healthy and Type II Diabetic Iraqi Patients Noor N.O. Al-Saad; Fayhaa M. Khaleel; Essam F. A. Al-Jumaili
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 3 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i3.15475

Abstract

Arginase was purified from human serum of healthy and patient with type II Diabetes mellitus subjects.The purification steps included, DEAE-cellulose chromatography and gel filtration on Sepharoe6B.Themolecular weight of native arginase was find to be (94 Kd), by gel filtration. The purified human serumarginase is characterized by optimum pH of (9.5), optimum pH of stability is between (9-10), optimumtemperature (350C), temperature of stability is between (30-500C) in both healthy and patient subjects.
Co-Authors Fayhaa M. Khaleel Noor N.O. Al-Saad