Purification and Characterization of Arginase from Serum of Healthy and Type II Diabetic Iraqi Patients

Noor N.O. Al-Saad
Fayhaa M. Khaleel
Essam F. A. Al-Jumaili

Indian Journal of Forensic Medicine & Toxicology
Vol. 15 No. 3 (2021): Indian Journal of Forensic Medicine & Toxicology

Arginase was purified from human serum of healthy and patient with type II Diabetes mellitus subjects.The purification steps included, DEAE-cellulose chromatography and gel filtration on Sepharoe6B.Themolecular weight of native arginase was find to be (94 Kd), by gel filtration. The purified human serumarginase is characterized by optimum pH of (9.5), optimum pH of stability is between (9-10), optimumtemperature (350C), temperature of stability is between (30-500C) in both healthy and patient subjects.