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All Journal Jurnal Ilmu Pertanian Indonesia Biopropal Industri
. Yopi
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Agriculture, Biological Sciences & Forestry Industrial & Manufacturing Engineering

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Karakteristik Susu Kerbau Sungai dan Rawa di Sumatera Utara Evy Damayanthi; . Yopi; Hasanatun Hasinah; Triana Setyawardani; Heni Rizqiati; Salwa Putra
Jurnal Ilmu Pertanian Indonesia Vol. 19 No. 2 (2014): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (469.226 KB)

Abstract

The objective of this research was to investigate the characteristics and differences in quality milk of Water andSwamp buffalo milk in North Sumatera. This research was conducted used complete random design used 30buffalo from three breeding farm in North Sumatera. Buffalo milk were collecting from lactating buffaloes on threetraditional farms located at Patumbak, Lubuk Pakam, and Siborong-borong with extensive farming system. Theresult of this research shown milk production per day for Swamp buffalo is 1 1.5 l and Water buffalo is 6 8 l withquality of Swamp and Water buffalo sequentially are for protein 5.14 ± 0.37% and 4.68 ± 0.41%, fat content 7.52 ±0.98% and 4.13 ± 0.73%, non-fat dry matter (NFDM) 10.61 ± 0.78% and 11.5 ± 0.86%, moisture 81.87 ± 2.26% and80.33 ± 2.33%, milk density 1.030 and 1.036, and then Total Plate Count (TPC) 3.79 x 106 and 5.08 x 105, shownindicated that there ware significant difference in protein, fat content and NFDM (P<0.01). But there is nodifferences in moisture and milk density. TPC shown that Swamp buffalo milk has above the maximum microbespresent in milk. The conclusion of this research is milk production of Swamp buffalo less than River buffalo but hashigher in chemical quality of milk than River buffalo such as protein, NFDM and fat content. Amino acids in Swampbuffalo milk were higher than River buffalo milk. Althought buffalo has less milk production than cow but buffalomilk were higher in all quality tested parameters and have more potential than dairy cow to be developed becausemaintenance of buffalo in North Sumatera is still use traditional method and not yet leading to efforts to obtainmaximum production performance.
PENGARUH KONSENTRASI KULIT SINGKONG DAN SUMBER NITROGEN TERHADAP PRODUKSI GLUKOAMILASE OLEH Aspergillus awamori KT-11PADA SUBMERGED FERMENTASI (Effect of Cassava Peelfor Glucoamylase Production by Aspergillus awamori KT-11 in Submerged Fermentation) Urip Perwitasari; S Amanah; M.D. Wahidiyah; . Nuryati; R Melliawati; L.N. Kholida; A. Andriani; A. Thontowi; A. Purnawan; A. Fathoni; S. Hartati; Y. Lusini; . Yopi
Biopropal Industri Vol 11, No 2 (2020)
Publisher : Balai Riset dan Standardisasi Industri Pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36974/jbi.v11i2.6079

Abstract

 The expensive price of glucoamylase is due to the high cost of enzyme production. Utilization biomass is expected to be an alternative raw material to reduce the cost of producing enzymes without reducing the quality of the product. Aspergillus awamori KT-11 is known to be able to produce glucoamylase by utilizing biomass waste. This study aimed to optimize glucoamylase production from A. awamori KT-11 by utilizing cassava peel as an alternative substrate with variation nitrogen source through Submerged Fermentation (SmF). Variables carry out in this study were the concentration of cassava peel (5-30%), nitrogen sources (casein hydrolyzate, yeast extract and sodium nitrate). The results showed in the concentration of cassava peels and nitrogen sources affect glucoamylase production. The optimum glucoamylase activity was 3984,935 U/L in 10% of cassava peel in medium. The other results showed different sources of nitrogen significantly influence glucoamylase production. The addition of yeast extract increased glucoamylase activity to 4617,894 U / L.Keywords: Aspergillus awamori KT-11, glucoamylase, cassava peel, submerged fermentation (SmF)ABSTRAKBahan baku yang mahal dalam produksi glukoamilase mengakibatkan harga jual glukoamilase tinggi. Pemanfaatan biomassa tinggi karbohidrat diharapkan dapat menjadi alternatif bahan baku untuk menurunkan biaya produksi enzim tanpa menurunkan kualitas dari produk yang dihasilkan. Aspergillus awamori KT-11 diketahui mampu memproduksi glukoamilase dengan memanfaatkan limbah biomassa. Tujuan penelitian ini adalah optimasi produksi glukoamilase dari A. awamori KT-11 dengan memanfaatkan limbah kulit singkong sebagai substrat alternatif dengan berbagai sumber nitrogen melalui Submerged Fermentation (SmF). Variabel yang dilakukan pada penelitian ini yaitu konsentrasi kulit singkong (5-30%), sumber nitrogen (kasein hidrolisat, yeast extract dan natrium nitrat). Hasil riset menunjukkan konsentrasi kulit singkong dan jenis sumber nitrogen mempengaruhi produksi glukoamilase. Aktivitas glukoamilase optimum diidentifikasi ketika konsentrasi substrat kulit singkong sebesar 10% yaitu 3984,935 U/L. Penambahan sumber nitrogen yang berbeda mempengaruhi produksi glukoamilase secara signifikan. Penambahan yeast extract meningkatkan aktivitas glukoamilase menjadi 4617,894 U/L.Kata kunci: Aspergillus awamori KT-11, glukoamilase, kulit singkong, submerged fermentasi (SmF)
Co-Authors . Nuryati A. Andriani A. Fathoni A. Purnawan A. Thontowi Evy Damayanthi Hasanatun Hasinah Heni Rizqiati L.N. Kholida M.D. Wahidiyah R Melliawati S Amanah S. Hartati Salwa Putra Triana Setyawardani Urip Perwitasari Y. Lusini