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All Journal Jurnal Fitopatologi Indonesia JURNAL AGROTEKNOLOGI
Sri Sulandari
Universitas Gadjah Mada
Agriculture, Biological Sciences & Forestry Medicine & Pharmacology

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Tomato yellow leaf curl Kanchanaburi virus Penyebab Penyakit Mosaik Kuning pada Tanaman Terung di Jawa Tega Kintasari; Dwi Wiyati Nurul Septariani; Sri Sulandari; Sri Hendrastuti Hidayat
Jurnal Fitopatologi Indonesia Vol 9 No 4 (2013)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (609.231 KB) | DOI: 10.14692/jfi.9.4.127

Abstract

Yellow and yellow mosaic symptoms was observed on eggplant (Solanum melongena) around Bogor, Bandung, Pati, Rembang, and Bantul during a survey in early 2013. Polymerase chain reaction based detection was carried out using total DNA isolated from symptomatic leaf samples and a pair of Begomovirus-universal primers, SPG1/SPG2. The expected size (~900 bp) amplicon was detected from all five symptomatic samples, indicating the presence of Begomovirus infection. Each amplicon was sequenced followed by basic local alignment search tool analysis. It was revealed that the sequence had the highest homology (98.8%) with Tomato yellow leaf curl Kanchanaburi virus (TYLCKaV) sequence. This is the first report on TYLCKaV infection on eggplant in Indonesia.
Identifikasi Molekuler Tobacco mosaic virus pada Anggrek di Sleman, Yogyakarta Soesamto Somowiyarjo; Sedyo Hartono; Sri Sulandari; Sekar Utami Putri
Jurnal Fitopatologi Indonesia Vol 12 No 2 (2016)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (298.833 KB) | DOI: 10.14692/jfi.12.2.69

Abstract

Tobamovirus is a group of virus with a wide host range, including orchid plant which considered as an economically important plant. This research aimed to identify Tobamovirus infecting orchids. Virus isolates were collected from orchid nursery in Sleman, Yogyakarta. Plant extract from orchid showing necrotic flex symptom was inoculated to indicator plants Chenopodium amaranticolor. Chlorotic local lesion symptoms occurred within 3 days after inoculation. RNA total from symptomatic C. amaranticolor was extracted by using a commercial kit. cDNA was synthesized using oligo d(T) primer. Amplification of cDNA using partial movement protein specific primers TMV-1F and TMV-2R was successfully amplified the amplicon with size ± 422 bp. The nucleotide sequences of this amplicon  showed highest DNA homology (98%) with Tobacco mosaic virus Yongren-2 isolat from China.
Deteksi Virus Terbawa Umbi Benih pada Bawang Merah Kultivar Biru Bantul Nurviani Nurviani; Sri Sulandari; Susamto Somowiyarjo; Siti Subandiyah
Jurnal Fitopatologi Indonesia Vol 12 No 5 (2016)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (558.07 KB) | DOI: 10.14692/jfi.12.5.185

Abstract

Virus infection on shallots may cause mosaic of leaves and plant dwarf.  It is very important to know the identity of the virus as a requirement in determining viral disease management strategy.  The research is subjected to identify seed- transmitted viruses from shallot bulbs. Detection of virus from bulb was conducted by growing on test, followed by mechanical inoculation on shallot cultivar Biru Bantul, Chenopodium amaranticolor, and Nicotiana tabacum and morphological observation of virus particle by electron microscope. About 2 weeks after inoculation symptoms was observed, i.e. mosaic and dwarf on shallot cultivar Biru Bantul, local necrotic on C. amaranticolor, but symptomless on N. tabacum.  Filamentous particles of 650 nm and 800 nm in length was observed under electron microscope from plants with mosaic and dwarfing symptom, respectively.  This indicated the presence of 2 different seed-transmitted viruses on infected shallots.  Two species of shallot viruses, i.e. Shallot latent carlavirus (SLV) and Onion yellow dwarf potyvirus (OYDV) was most likely the main seedborne viruses on shallot bulb.  Identification based on nucleic acid and protein sequence analysis is necessary for further confirmation
Identifikasi Molekuler Cowpea mild mottle virus pada Tanaman Kedelai di Jawa Umi Kulsum; Sedyo Hartono; Sri Sulandari; Susamto Sumowiyarjo
Jurnal Fitopatologi Indonesia Vol 12 No 6 (2016)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (715.643 KB) | DOI: 10.14692/jfi.12.6.224

Abstract

Soybean (Glycine max) is an important  food crops in Indonesia, but the production is considered low. Infection of Cowpea mild mottle virus (CPMMV) may affect to the productivity of the crops.  The virus cause a variety of symptoms on soybean such as mosaic, chlorosis, necrosis, distortion, and leaf dwarf. The aims of this study were to detect and identify CPMMV by molecular technique from some varieties of soybeans, i.e.  ‘Anjasmoro’, ‘Argomulyo’, ‘Grobogan’, and two local Edamame varieties. Symptomatic samples was obtained randomly from main soybean growing areas in Java.  DNA amplification using specific primer for coat protein of CPMMV was only successfully gained from leaf samples of var. Argomulyo from Malang which showing mosaic on the leaf surface, vein clearing, and chlorosis. Further amino acid and nucleotide sequence analysis showed that this CPMMV isolate has highest homology (99%) to CPMMV isolate of French bean from China.
Cucumber mosaic virus pada Tanaman Lada di Yogyakarta dan Bangka Belitung Emerensiana - Uge; sri sulandari; sedyo - hartono; susamto - somowiyarjo
Jurnal Fitopatologi Indonesia Vol 15 No 1 (2019)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (835.439 KB) | DOI: 10.14692/jfi.15.1.1

Abstract

Cucumber mosaic virus on Black Pepper in Yogyakarta and Bangka BelitungPepper  (Piper  nigrum)  is  spice  crop  which  has  been  cultivated  a  long  time  ago  in  Indonesia. Stunting is one of disease on pepper caused by cucumber mosaic virus (CMV). The research aimed to diagnose the biological, morphological and nucleaic acid characters of CMV on pepper in Yogyakarta and Bangka Belitung. CMV infection on pepper in both area (Putat dan Kleben village, Yogyakarta and Air Buluh village, Bangka Belitung) showed typical symptoms such as mosaic, narrow leaves and stunting. The disease incidence and disease severity of stunting disease are varies. The virus able to transmitted by cutting, grafting and mechanically on Nicotiana tabacum and Chenopodium amaranticolor. However, it was unable to transmitted mechanically on pepper and by Aphis gossypii. The virus particles were isometric with diameter size 28-30 nm. RT-PCR using coat protein partial gene primer successfully amplified a DNA with size ± 500 bp from all three samples. The homology of nucleotide between three isolates was 98-97%, while the highest homology of those three strains CMV  from Yogyakarta and Bangka Belitung was 98% against strains from China in Brassica chinensis. Three strains CMV from pepper were in the same group, and separated from CMV pepper lines from Indonesia and other CMV isolates. 
Diversity of Rhizoctonia solani Isolates of Rice Varieties of Ciherang, IR 64, Mekongga, and Situ Bagendit Arina Manasikana; Sri Sulandari; Achmadi Priyatmojo
Jurnal Fitopatologi Indonesia Vol 17 No 4 (2021)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.17.4.141-150

Abstract

Padi (Oryza sativa) termasuk ke dalam komoditas penting di Indonesia. Salah satu penyakit penting pada tanaman padi ialah penyakit hawar pelepah yang disebabkan oleh Rhizoctonia solani. Tujuan penelitian ini untuk mengetahui karakteristik dan kelompok anastomosis R. solani yang diisolasi dari tanaman padi varietas Ciherang, IR 64, Mekongga, dan Situ Bagendit; dan mengetahui keragaman genetiknya menggunakan primer universal, dan tingkat kekerabatannya. Penelitian dilaksanakan pada Oktober 2019 hingga Juli 2020 di Laboratorium Teknologi Pengendalian UGM. Pengambilan sampel dilaksanakan di Kecamatan Pandak, Bantul yang selanjutnya dilakukan isolasi dan pemurnian cendawan Rhizoctonia menggunakan medium agar-agar dekstrosa kentang. Isolat R. solani diklasifikasikan berdasarkan keragaman kultur, keragaman morfologi, jumlah inti sel, kemampuan anastomosis (AG), dan keragaman genetik. Analisis keragaman genetik dilakukan dengan PCR menggunakan primer universal ITS1 dan ITS4. Berdasarkan pengamatan keragaman kultur dan keragaman morfologi diperoleh hasil yang bervariasi. Hasil pengamatan jumlah inti sel pada keseluruhan isolat berkisar antara 5 hingga 7 inti pada sel yang termasuk dalam kategori multinukleat. Pengamatan kelompok anastomosis (AG) pada 13 isolat yang digunakan masuk ke dalam kategori C3 (anastomosis sempurna). Analisis PCR diperoleh pita DNA dengan hasil sesuai target yaitu 600–750 pb. Hasil secara sikuensing diketahui bahwa 12 isolat R. solani menunjukkan kekerabatan yang tinggi dengan isolat AG-1 IA, kecuali pada isolat CH 3.
Molecular Identification of DNA Satellite Associated with Mungbean yellow mosaic India virus infecting Yardlong Bean in Yogyakarta Mustika Ajeng Kartini Putri Pertiwi; Sedyo Hartono; Susamto Somowiyarjo; Sri Sulandari; Argawi Kandito
Jurnal Fitopatologi Indonesia Vol 17 No 6 (2021)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.17.6.251-260

Abstract

Gejala mosaik kuning dan keriting daun ditemukan pada pertanaman kacang panjang di Sleman, Yogyakarta. Begomovirus diketahui sebagai salah satu penyebab penyakit tersebut. Penelitian dilakukan untuk mengidentifikasi spesies Begomovirus dan DNA satelit yang berasosiasi dengan penyakit mosaik kuning kacang panjang. Ekstraksi DNA total dari tanaman bergejala dilanjutkan dengan amplifikasi fragmen DNA spesifik Begomovirus dan Betasatelit. Amplikon DNA berukuran ±1500 pb dan ±1300 pb berhasil diperoleh menggunakan berturut-turut primer universal Begomovirus dan primer spesifik Betasatelit. Analisis sekuen nukleotida mengonfirmasi identitas Begomovirus yang menginfeksi tanaman kacang panjang ialah Mungbean yellow mosaic India virus (MYMIV) dengan homologi 99% terhadap isolat MYMIV asal Indonesia. DNA satelit yang berasosiasi dengan MYMIV menunjukkan karakteristik Betasatelit, yaitu memiliki satellite common region (SCR) dengan struktur stem-loop dan sekuen TAATATTAC pada bagian loop, adenine rich region sebesar 54.96%, dan ORF (open reading frame) non-coding. Lebih lanjut, analisis rekombinasi menggunakan SimPlot mengindikasikan bahwa satelit non-coding MYMIV merupakan satelit rekombinan antara Betasatelit dan DNA-B Pepper yellow leaf curl Indonesia virus (PepYLCIV). Artikel ini merupakan laporan pertama asosiasi betasatelit DNA non-coding dengan MYMIV di Indonesia.
POTENTIAL EFFECT OF RHIZOBACTERIA Streptomyces sp. AND Bacillus sp. TO GEMINIVIRUS INFECTION AND RED CHILI (Capsicum annum L.) QUALITY PRODUCTION Azizah Ridha Ulilalbab; Sri Sulandari; Triwidodo Arwiyanto; Sedyo Hartono
Jurnal Agroteknologi Vol 14, No 1 (2023): Agustus 2023
Publisher : Universitas Islam Negeri Sultan Syarif Kasim Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24014/ja.v14i1.24689

Abstract

The chili yellow leaf curl disease is one of the biggest obctacle in chili’s production in Indonesia. This diseases only transmitted by whitefly (Bemisia tabaci). One of the disease controlling method is by using Plant Growth Promoting Rhizobacteria (PGPR) to reduce pesticide application for the health and environment. The aim of this research is to find out the effectiveness of Streptomyces sp. and Bacillus sp. in supressing yellow leaf curl disease, plant growth and chili harvest. This research has conducted at the experimental farmer’s field in Harjobinangun, Pakem, Sleman, Special Region of Yogyakarta. Using varieties of chili Twist 42, Streptomyces isolates which from Bacteriology Laboratory collection’s with 10-8 CFU/ml and Bacillus isolates which is from Mycrobiology Laboratory collection with  10-8 CFU/ml. The result of this research showed that the treatment of combination between Bacillus sp. and Streptomyces sp. could decrease and supress disease incidence and disease intensity if it compared with others treatment. Parameters of plant growth included plant high, stem diameter, root length, root volume and number of productive branch showed that single of Streptomyces sp. and Bacillus sp. without combination were better than combination and untreated.  The single application Streptomyces sp. or Bacillus sp. could increase amount of total number and weight of fruits.
Co-Authors Achmadi Priyatmojo Argawi Kandito Arina Manasikana Azizah Ridha Ulilalbab Dwi Wiyati Nurul Septariani Emerensiana - Uge Mustika Ajeng Kartini Putri Pertiwi Nurviani Nurviani SEDYO HARTONO Sekar Utami Putri Siti Subandiyah Soesamto Somowiyarjo SRI HENDRASTUTI HIDAYAT susamto - somowiyarjo Susamto Somowiyarjo Susamto Sumowiyarjo Tega Kintasari Triwidodo Arwiyanto Umi Kulsum