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All Journal Jurnal Fitopatologi Indonesia
Susamto Somowiyarjo
Universitas Gadjah Mada
Medicine & Pharmacology

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Deteksi Virus Terbawa Umbi Benih pada Bawang Merah Kultivar Biru Bantul Nurviani Nurviani; Sri Sulandari; Susamto Somowiyarjo; Siti Subandiyah
Jurnal Fitopatologi Indonesia Vol 12 No 5 (2016)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (558.07 KB) | DOI: 10.14692/jfi.12.5.185

Abstract

Virus infection on shallots may cause mosaic of leaves and plant dwarf.  It is very important to know the identity of the virus as a requirement in determining viral disease management strategy.  The research is subjected to identify seed- transmitted viruses from shallot bulbs. Detection of virus from bulb was conducted by growing on test, followed by mechanical inoculation on shallot cultivar Biru Bantul, Chenopodium amaranticolor, and Nicotiana tabacum and morphological observation of virus particle by electron microscope. About 2 weeks after inoculation symptoms was observed, i.e. mosaic and dwarf on shallot cultivar Biru Bantul, local necrotic on C. amaranticolor, but symptomless on N. tabacum.  Filamentous particles of 650 nm and 800 nm in length was observed under electron microscope from plants with mosaic and dwarfing symptom, respectively.  This indicated the presence of 2 different seed-transmitted viruses on infected shallots.  Two species of shallot viruses, i.e. Shallot latent carlavirus (SLV) and Onion yellow dwarf potyvirus (OYDV) was most likely the main seedborne viruses on shallot bulb.  Identification based on nucleic acid and protein sequence analysis is necessary for further confirmation
Molecular Identification of DNA Satellite Associated with Mungbean yellow mosaic India virus infecting Yardlong Bean in Yogyakarta Mustika Ajeng Kartini Putri Pertiwi; Sedyo Hartono; Susamto Somowiyarjo; Sri Sulandari; Argawi Kandito
Jurnal Fitopatologi Indonesia Vol 17 No 6 (2021)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.17.6.251-260

Abstract

Gejala mosaik kuning dan keriting daun ditemukan pada pertanaman kacang panjang di Sleman, Yogyakarta. Begomovirus diketahui sebagai salah satu penyebab penyakit tersebut. Penelitian dilakukan untuk mengidentifikasi spesies Begomovirus dan DNA satelit yang berasosiasi dengan penyakit mosaik kuning kacang panjang. Ekstraksi DNA total dari tanaman bergejala dilanjutkan dengan amplifikasi fragmen DNA spesifik Begomovirus dan Betasatelit. Amplikon DNA berukuran ±1500 pb dan ±1300 pb berhasil diperoleh menggunakan berturut-turut primer universal Begomovirus dan primer spesifik Betasatelit. Analisis sekuen nukleotida mengonfirmasi identitas Begomovirus yang menginfeksi tanaman kacang panjang ialah Mungbean yellow mosaic India virus (MYMIV) dengan homologi 99% terhadap isolat MYMIV asal Indonesia. DNA satelit yang berasosiasi dengan MYMIV menunjukkan karakteristik Betasatelit, yaitu memiliki satellite common region (SCR) dengan struktur stem-loop dan sekuen TAATATTAC pada bagian loop, adenine rich region sebesar 54.96%, dan ORF (open reading frame) non-coding. Lebih lanjut, analisis rekombinasi menggunakan SimPlot mengindikasikan bahwa satelit non-coding MYMIV merupakan satelit rekombinan antara Betasatelit dan DNA-B Pepper yellow leaf curl Indonesia virus (PepYLCIV). Artikel ini merupakan laporan pertama asosiasi betasatelit DNA non-coding dengan MYMIV di Indonesia.
Co-Authors Argawi Kandito Mustika Ajeng Kartini Putri Pertiwi Nurviani Nurviani SEDYO HARTONO Siti Subandiyah Sri Sulandari