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Journal : Jurnal Perlindungan Tanaman Indonesia

Peranan Faktor Cuaca terhadap Infeksi dan Perkembangan Penyakit Bercak Ungu pada Bawang Merah Bambang Hadisutrisno; Sudarmadi Sudarmadi; Siti Subandiyah; Achmadi Priyatmojo
Jurnal Perlindungan Tanaman Indonesia Vol 1, No 1 (1995)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.9351

Abstract

Purple blotch disease caused by Alternaria porri (Ell.) Cif. is one of the most important diseases on shallots. The farmers have been using fungicides as a reliable control method, however, the chemical control method has to be considered for the negative effect on the environment and the danger to the other species and causes the resistance to a certain pesticide. Therefore, it is important to develop other method that is safe, effective, and efficient, like the use of climatic data to decide the control strategy. The result of the study on ecology of purple blotch disease carried out at the experimental station in Kramat-Tegal, Kledung-Temanggung, and KP4 Kalitirto in dry and rainy season (1991/1992) suggested that climatic factors played on important role on the daily conidium dissemination of Alternaria porri and on the intensity of purple blotch disease. Daily conidium dissemination which was one of the important component in the epidemy of purple blotch disease was influenced by the temperature, relative humidity, and the wind velocity. Conidium dissemination was occurred both during the day and night and at 10.00 am-14.00 pm when the air temperature and wind velocity were high with low relative humidity, conidium dissemination was maximum. On the other hand, at 22.00 pm – 02.00 am there was minimum conidium dissemination. The result on the effects of leaf wetness period on disease intensity suggested that high humidity was not the only factor caused disease infection but it has to be supported by the presence of thin film water on the leaf surface at least for 4 hours since the attachment of the conidia on the leaves.
Ekstraksi DNA Phytophthora palmivora Isolat Kelapa Achmadi Priyatmojo; Siti Subandiyah
Jurnal Perlindungan Tanaman Indonesia Vol 2, No 1 (1996)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (6570.787 KB) | DOI: 10.22146/jpti.9358

Abstract

Coconut isolate of Phytophthora palmivora DNA was extracted from sporangium and mycelium using SDS and Proteinase-K method. Sporangium and mycelium were harvested from liquid and solid culture of Potato Dextrose medium. The result of the study showed that the DNA extracted from sporangium and mycelium of 8 days solid culture was seen as distinct band. On the other hand, the dNA from sporangium and mycelium of 24 days old liquid culture was fragmented with smear band appearance.
Hubungan Antara Inokulum Awal Patogen dengan Perkembangan Penyakit Hawar Upih pada Padi Varietas Ciherang Bambang Nuryanto; Achmadi Priyatmojo; Bambang Hadisutrisno; Bambang Hendro Sunarminto
Jurnal Perlindungan Tanaman Indonesia Vol 16, No 2 (2010)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.11724

Abstract

Study on the role of initial inoculums on disease development of rice sheath blight caused by Rhizoctonia solani Kühn was conducted in glass house at the Faculty of Agriculture University of Gadjah Mada from December of 2009 to July of 2010. The aim of this study was to evaluate the importance of sclerotia and mycelium in plant debris as primary inoculum form of R. solani. Results indicated that both sclerotia and mycelium in plant debris significantly affect the development of rice sheath blight. Disease severity was closely related to the number of sclerotia on rice plant. The highest disease severity was observed in plant inoculated with 10 sclerotia per hills. Treatments of 6 sclerotia and 5 g plant debris per hills had comparable effect on disease severity and area under disease progress curve of rice sheath blight. The results suggested that sclerotia and mycelia in plant debris might play a major role as primary inocula of the disease in rice growing field.
Penapisan PGPF untuk Pengendalian Penyakit Busuk Lunak Lidah Buaya (Aloe vera) di Tanah Gambut Supriyanto Supriyanto; Achmadi Priyatmojo; Triwidodo Arwiyanto
Jurnal Perlindungan Tanaman Indonesia Vol 15, No 2 (2009)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.11768

Abstract

Aloe (Aloe vera) planted in West Borneo peat soil is well known as having the best product quality in Indonesia. Soft rot disease is one of the constraints of Aloe cultivation on peat soil. Many methods have no significant result for controlling this disease. This research objectives are to obtain Plant Growth Promoting Fungi (PGPF) for controlling Aloe bacterial soft rot in peat soil. The research was conducted in Clinical Laboratory of Plant Pathology and glass house of Faculty of Agriculture, Gadjah Mada University, Yogyakarta from October 2008 to September 2009. The methods included fungal isolation from peat land, hypovirulent and PGPF ability test, and biological control test in the glass house. Among 42 peat soil fungi tested, 28 isolates were hypovirulent and only 2 isolates i.e. SNTH001 (Penicillium sp.) and SNTH003 (Aspergillus sp.) showed the PGPF ability. Glass house trial showed that single application of SNTH001 and SNTH003 isolates were able to increase the growth of Aloe. In the biological control of Aloe soft rot disease test showed that the lowest intensity (25%) might be obtained by using SNTH001 isolate.
Penekanan Penyakit Lincat Tembakau Temanggung dengan Streptomyces spp. Triwidodo Arwiyanto; Kharis Haryono; Achmadi Priyatmojo; Toekidjo Martoredjo; Gembong Dalmadiyo
Jurnal Perlindungan Tanaman Indonesia Vol 13, No 1 (2007)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.11777

Abstract

Streptomyces is one of a soil microorganisms which is rarely used by Indonesian researcher for biological control of planth pathogent. Local isolates of Streptomyces spp. were used in this study to control "lincat disease" of Temanggung tobacco. Ninety four isolates of Streptomyces were tested directly in the field to suppress the development of lincat disease in the field. Six isolates, i.e. Stre-4, Stre-7,Stre-48, Stre-61, Stre-66 and Stre-67 suppressed the disease development with various degree. It was likely that decreasing the concentration of bacterial antagonist gave better protection against the disease. In vitro experiments indicated that there was no correlation between inhibition pathogen in vitro and ability to suppress disease in the field. The result showed that selection of antagonist in vitro based on antibiosis will eliminate the chance to get other potential antagonist.
Identifikasi Morfologi Beberapa Spesies Jamur Fusarium Ade Mahendra Sutejo; Achmadi Priyatmojo; Arif Wibowo
Jurnal Perlindungan Tanaman Indonesia Vol 14, No 1 (2008)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.11870

Abstract

The research was conducted to study the morphological difference based on macroscopic and microscopic appearance of several Fusarium spp. Fusarium spp. isolates were propagated onto Potato Dextrose Agar (PDA) by using single-spore method. All isolates were observed macroscopically and microscopically to determine colony appearance, colony growth diameter and formation of macroconidia, microconidia and conidiophores. The results showed that colony appearance of all isolates was similar. Therefore they could not clearly be differentiated by one to another. On the other hand, microscopic observation showed that there was different conidiophore morphology of F. oxysporum and other Fusarium spp. Microscopic morphology among F. oxysporum isolates were difficult to differentiate. Microconidia were produced in false-head which was the characteristic feature of most F. oxysporum. In conclusion, microscopic morphology observation could only be able to differentiate Fusarium spp. isolates at species level, but not to formae speciales.
Tipe Mating pada Empat Isolat Thanatephorus Cucumeris Anamorf: Rhizoctonia solani) Anastomosis Group (AG) 1-IC Achmadi Priyatmojo
Jurnal Perlindungan Tanaman Indonesia Vol 12, No 2 (2006)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.12077

Abstract

Four parent isolates (189, Rh28, BW3 and F-1) of Thanatephorus cucumeris (anamorph: Rhizoctonia solani) AG 1-IC were induced to produce basidiospores using soil-over culture method. All of four parent isolates could produce basidiospores. Colonies obtained from single basidiospore isolate of each parent isolate were paired on charcoal potato dextrose agar. Single basidiospore isolate having different mating type produced tuft at area of the junction of paired colonies. On the based of tuft formation, single basidiospore isolates of each parent isolate could be divided into two different mating types, therefore it is concluded that each of 189, Rh28, BW3 and F-1 isolate of T. cucumeris AG I-IC has bipolar mating type.