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Immobilization of Lipase on Surfactant-Modified Bentonite and Its Application for Biodiesel Production from Simulated Waste Cooking Oil Chrisnasari, Ruth; Yonardi, Angelina; Lie, Hesti; Widi, Restu Kartiko; Purwanto, Maria Goretti Marianti
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UNEJ e-Proceeding

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Abstract

The influence of bentonite modification by cationic surfactant hexadecyl-trimethyl-ammonium bromide (HDTMA-Br) and tetramethyl ammonium hydroxide (TMAOH) on its capability to immobilize lipase was studied. Modification of bentonite was conducted by the adding of 4-6% (v/v) HDTMA-Br and TMAOH respectively. The obtained immobilized lipases then were characterized to observe the optimum pH and temperature as well as their stability during reuse application. The observed results show that there is no significant difference between the variations of HDTMA-Br concentrations to the percentage of immobilized enzyme which can immobilize lipase up to 75-78%. However, the best concentration of TMAOH is 4% (v/v) which can immobilize lipase up to 97.95%. The obtained immobilized lipases on HDTMA-Br-modified bentonite show the optimum catalytic activity on reaction temperature of 35-40 oC and pH of 7.5. In other hand, the optimum catalytic activity of immobilized lipases on TMAOH-modified bentonite is 40oC of incubation temperature and pH of 7. The immobilized lipases on both HDTMA-Br and TMAOH modified bentonite are stable enough so it could be re-used four times before its activity decreased by 48,565% and 46.83 % respectively. Keywords: Lipase, Cationic Surfactant, Bentonite, HDTMA-Br, TMAOH, Biodiesel, Immobilization.
Viability and Sublethal Detection of Probiotic Bacteria on Instant Freeze-Dried Fermented Soy Milk (Study on Isolate Type and Sucrose Concentration as Cryoprotectant) Nisa, Fithri Choirun; Wardani, Agustin Krisna; Chrisnasari, Ruth
Jurnal Teknologi Pertanian Vol 9, No 1 (2008)
Publisher : Fakultas Teknologi Pertanian Universitas Brawijaya

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Abstract

Fermented soy milk is one of probiotic beverage, contain high protein and isoflavon that ha healthy effect. However, the usage of soy milk as fermented product is not effective because needs low temperature. To overcome this problem is by converting the fermented soy milk into instant dried product.  However, freeze drying can reduce the viability of probiotic.   The aim of this research was to determine the isolate and sucrose concentration to obtain high viability of probiotic. This research was conducted in randomized block design with two factors. The first factor was type of isolate that consist of L. acidophilus, L. casei, and L. plantarum. The second factor was sucrose concentration (5% and 10%). Each treatment was done in four replications.The result showed significant difference of kind isolate treatment on reducing sugar, protein, total acid, pH, and total LAB during fermentation with total LAB and sub lethal on dried product, but no significant difference on total sugar and N-amino. Sucrose concentration treatment showed significant difference on total LAB, sub lethal, water content, and reserve ability water vapor, but no significant difference on solubility. Interaction of both treatments gave significant difference on total LAB and sub lethal.  The best treatment resulted from the combination of 5% sucrose concentration and isolate L. acidophilus.Keywords: probiotic, cryoprotectant, freeze drying, instant fermented soy milk
Karakterisasi Enzim Pemecah Pati dari Malt Serelia Phieter, Alvina Cornelia; Chrisnasari, Ruth; Pantjajani, Tjandra
Keluwih: Jurnal Sains dan Teknologi Vol. 1 No. 1 (2020): Keluwih: Jurnal Sains dan Teknologi (February)
Publisher : Direktorat Penerbitan dan Publikasi Ilmiah, Universitas Surabaya

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Abstract

Abstract- Starch-degrading enzymes not only can be found in bacteria and fungi, but also in plants. Some plants that produce starch-degrading enzymes are germinated grain of sorghum, maize, and mung bean. pH and temperature are factors that can affect the activity of enzyme. Effect of pH and temperature to starch-degrading enzyme activity of these 3 cereal grains are reported in this research. Grain of sorghum, maize, and mung bean were germinated for 2 days and dried to produce malt. Enzymes from these 3 different malts were extracted using 7 buffers with different pH (4.5, 5, 5.5, 6, 6.5, 7) . Buffer that produced highest enzyme activity based on degradation of starch as a substrate (iodine-starch method) and based on formation of reducing sugars as products (DNS method) would be used for determining the effect of temperatures (20 °C, 30 °C, 40 °C, 50 °C). Effect of pH and temperatures to enzyme activity from 3 different malts tend to be fluctuating. Sorghum malt had the highest enzyme activity per gram malt based on degradation of starch activity test. Estimated enzyme activity of sorghum malt was 103,82 mg.g- 1.min-1. Maize malt had the highest enzyme activity based on formation of reducing sugars activity test. Estimated enzyme activity of maize malt was 13.08 mg.g-1.min-1. Keywords: amylase enzyme, sorghum malt, maize malt, mung bean malt, pH temperature Abstrak- Enzim pemecah pati dapat diperoleh dari tanaman selain dari bakteri dan fungi. Beberapa jenis tanaman yang memiliki enzim pemecah pati adalah biji sorgum, jagung, dan kacang hijau yang berkecambah. pH dan suhu merupakan beberapa faktor yang mempengaruhi aktivitas dari enzim. Pengaruh pH dan suhu terhadap aktivitas enzim amilase dari ketiga jenis biji ini akan diamati dalam penelitian ini. Biji sorgum, jagung, dan kacang hijau dikecambahkan selama 2 hari dan dikeringkan untuk menghasilkan malt. Enzim dari ketiga jenis malt ini akan diekstrak menggunakan 7 buffer pH berbeda yaitu buffer pH 4,5; 5; 5,5; 6; 6,5 dan 7. Buffer yang menghasilkan nilai aktivitas enzim tertinggi berdasarkan degradasi substrat pati (metode pati-iodin) dan berdasarkan pembentukan produk gula reduksi (metode DNS) akan digunakan lebih lanjut untuk melihat pengaruh suhu yaitu suhu 20 °C, 30 °C, 40 °C, dan 50 °C. Pengaruh pH dan suhu terhadap aktivitas enzim ketiga jenis malt cenderung fluktuatif. Malt sorgum memiliki nilai aktivitas enzim per gram malt tertinggi berdasarkan uji aktivitas degradasi substrat pati (metode pati-iodin) yaitu 103,82 mg.g-1.min-1 dan malt jagung memiliki nilai aktivitas enzim per gram malt tertinggi berdasarkan uji aktivitas pembentukan gula reduksi (metode DNS) yaitu 13,08 mg.g-1.min-1. Kata kunci: amylase enzyme, sorghum malt, maize malt, mung bean malt, pH temperature
Pengaruh Pengolahan Terhadap Nilai Fungsional Bawang Putih (Allium sativum) Thalia, Chessa Uly; Chrisnasari, Ruth; Rosita Dewi, Ardhia Dheasy
Keluwih: Jurnal Sains dan Teknologi Vol. 1 No. 1 (2020): Keluwih: Jurnal Sains dan Teknologi (February)
Publisher : Direktorat Penerbitan dan Publikasi Ilmiah, Universitas Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (542.216 KB) | DOI: 10.24123/saintek.v1i1.2782

Abstract

Abstract-Organosulphur compounds in garlic has bactericidal effect and antioxidant potentials. However, toxic effects and strong odor produced by the compound allicin contribute to the reduction of garlic consumption. The production of allicin compound can be inhibited by skipping grinding and substituting it with other methods such as heating with oven and soaking in salt water, so the production pathway of organosulphur compounds will produce S-allyl cysteine which have lower toxic effect and antioxidant activity up to six fold compared to allicin. This research is done as a preliminary test to know the effect of process towards functional value of garlic. The results showed that the best treatment for black garlic (80℃ for 40 days) and pickled garlic (salt concentration of 3% for 40 days) has antioxidant activity (37,03%, 72,30%, and 32,17%), phenolic content (0,50mg GAE/g sample, 1,09mg GAE/g sample, and 0,146mg GAE/g sample), vitamin C content (0,068% b/b, 0,348% b/b, and 0,054% b/b), and protein content (3,1% N, 7,61% N, and 1,45% N) respectively that was significantly different (pvalue < 0,05), antimicrobial activity and total plate count test was not significantly different (pvalue > 0,05). Therefore, the process that gives the highest functional value was the black garlic. Keywords: garlic, black garlic, pickled garlic, functional food, antioxidant activity, phenols, alliin, S-allyl cysteine Abstrak-Senyawa organosulfur dalam bawang putih memiliki efek bakteriosidal dan potensi sebagai antioksidan. Namun, adanya efek toksik dan bau menyengat yang dihasilkan oleh senyawa allisin mengurangi daya konsumsi masyarakat. Produksi senyawa allisin dapat dihambat dengan menghilangkan penggerusan dan melakukan pengolahan yang lain, yaitu pengovenan dan perendaman pada air garam, sehingga jalur produksi senyawa organosulfur akan menghasilkan S-allyl cysteine dengan efek toksik jauh lebih kecil namun aktivitas antioksidan enam kali lebih tinggi dibandingkan allisin. Penelitian ini sebagai uji pendahuluan untuk mengetahui pengaruh pengolahan terhadap nilai fungsional bawang putih. Dari hasil uji, bawang putih, perlakuan terbaik bawang hitam (suhu 80℃ selama 40 hari), dan pickled garlic (kadar garam 3% selama 40 hari) secara berturut-turut memiliki aktivitas antioksidan (37,03%, 72,30%, dan 32,17%), senyawa fenolik total (0,50mg GAE/g sampel, 1,09mg GAE/g sampel, dan 0,146mg GAE/g sampel), kadar vitamin C (0,068% b/b, 0,348% b/b, dan 0,054% b/b), dan kadar protein (3,1% N, 7,61% N, dan 1,45% N) yang berbeda signifikan (pvalue < 0,05), aktivitas antimikroba dan uji total plate count tidak berbeda signifikan (pvalue > 0,05), sehingga pengolahan yang memberikan nilai fungsional tertinggi yaitu bawang hitam. Kata kunci: bawang putih, bawang hitam, pickled garlic, pangan fungsional, aktivitas antioksidan, fenolik, alliin, S-allyl cysteine.