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All Journal BERITA BIOLOGI Manilkara: Journal of Bioscience
Diyah Martanti
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Neuroscience Veterinary

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ANALISIS KERAGAMAN GENETIK KENTANG HITAM Kusumadewi Sri Yulita; Fajarudin Ahmad; Diyah Martanti; Yuyu S. Poerba; Herlina Herlina
BERITA BIOLOGI Vol 13, No 2 (2014)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v13i2.686

Abstract

Kentang hitam [Plectranthus rotundifolius (Poiret) Sprengel] of family Lamiaceae is a minor root crop known only for people living in some parts of Java, Bali and Madura. It was rarely found in its natural habitat, thus it was assumed to have low level of genetic diversity. This present study aimed to assess genetic diversity of 63 accessions of kentang hitam from provenances of Java based on Inter Simple Sequence Repeats (ISSR) and Random Amplified Polymorphic DNA (RAPD) fingerprints. Ten primers of ISSR and RAPD were initially screened and eight were selected for the analysis. These eight primers (OPA13, OPB10, OPB13, OPD8, OPN14, UBC 807, 834 and 835) generated 61 bands with an average of 7.63 polymorphic fragment per primer. Percentage of polymorphism ranged from 8.20% (UBC 807 and 834) to 16.39% (OPB 10) with an average of 12.50%polymorphism.Clustering analysis was performed based on ISSR and RAPD profiles using the neighbour joining method and Principle Coordinate Analysis (PCO). The range of genetic similarity among accessions was 51-100% to which most of the accessions were clustered with more than 80% similarity. This confirmed our hypothesis of the low level of variation existed among accessions.
Identifikasi Tingkat Ploidi Tanaman Pisang Menggunakan Flow Cytometer di Jawa Tengah Herlina; Yuyu Suryasari Poerba; Tri Handayani; Fajarudin Ahmad; Diyah Martanti
MANILKARA: Journal of Bioscience Vol 2 No 2 (2024): Februari
Publisher : LPPM Universitas Terbuka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33830/manilkara.v2i2.7573.2024

Abstract

In Indonesia, banana plants are known to have high genetic diversity, both cultivated and wild bananas. Banana plants are naturally known to have several levels of ploidy, namely diploid, triploid, and tetraploid. This study aims to identify the level of ploidy in banana plants explored in Central Java. The research method used flow cytometer, which analyzes the size of the plant genome, thus providing an overview of the level of ploidy and genetic diversity in banana plant populations. The research samples were banana plant leaves from various exploration locations in Central Java. The identification results show that of the 30 banana plant leaf samples that were successfully analyzed, it is known that the largest number of samples from banana plants that have triploid ploidy level (2n = 3x), namely 15 numbers, diploid ploidy level (2n = 2x) as many as 14 numbers, and found 1 cultivated banana with tetraploid ploidy level (2n = 4x). The results of ploidy level identification can provide guidance for researchers and plant breeders in selecting the right genotype for the development of superior varieties.
Co-Authors Fajarudin Ahmad Fajarudin Ahmad Herlina Herlina Herlina Kusumadewi Sri Yulita Tri Handayani Yuyu S. Poerba Yuyu Suryasari Poerba