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All Journal Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati BERITA BIOLOGI Manilkara: Journal of Bioscience
Yuyu Suryasari Poerba
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Neuroscience Veterinary

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Keragaman Genetik Kultivar Pisang Diploid (AA) Koleksi Cibinong Science Center Berdasarkan Marka RAPD dan ISSR Yuyu Suryasari Poerba; Fajarudin Ahmad
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 3 (2010): October 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v15i3.2584

Abstract

The banana (Musa acuminata Colla) is considered as an important crop plant due to its high economic value which also has good dietary source. Here, the genetic variation of 20 diploid (AA) banana cultivars from Cibinong Science Center collection were analyzed. Random amplified polymorphic DNAs (RAPDs) and Inter Simple Sequence Repeats fingerprinting of these banana cultivars were carried out by four primers of RPDSs and two primers of inter simple sequence repeats (ISSRs) led to DNA amplification. The amplification products of RPADs and ISSRs were polymorphic, 97.83% and 95%, respectively. Size of the bands was varied from 350bp to 2.0 kbp. The range of genetic distance was from 0.06 to 0.07. The molecular data showed that these banana varieties were diverse collection.
Analisis Keragaman Genetik Dyera costulata (Miq) Hook.f. Berdasarkan Marka “Random Amplified Polymorphic DNA” Yuyu Suryasari Poerba; Elizabeth A. Widjaya
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 1 (2010): February 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v15i1.2657

Abstract

Dyera costulata (Miq.) Hook.f (Apocynaceae) is a large tree of the lowland tropical rain forest of Southeast Asia that occurs in Thailand, the Malay Peninsula and on the islands of Sumatera and Borneo. Its economic value was in its latex, used as gum chile in the manifacture of chewing gum. Nowadays the timber of this species is largely utilized for the manufacture of pencils and picture frames. The information on genetic diversity of the species is very limited. Hence studies were initiated and genetic diversity were estimated using Random Amplified Polymorphic (RAPD) markers in 47 accessions of Dyera costulata procured from different geographical regions of Jambi. Four selected Operon primers (10 mer) generated a total of 90 consistent amplification products ranging from 150 bp to 2.8 Kb. The cluster analysis showed that the 47 individuals were separated into one main cluster and one individual. The range of genetic dissimilarity value among samples was from 0.06 to 0.71, while genetic distance among populations was from 0.17 to 0.42. These values showed that those 47 accessions of D. costulata from Jambi was genetically originated from diverse population.
Induksi Mutasi Curcuma zedoaria (Christm) Roscoe dengan Irradiasi Sinar Gamma Yuyu Suryasari Poerba; Diah Martanti
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 14, No 2 (2009): June 2009
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v14i2.2686

Abstract

Curcuma zedoaria (Zingiberaceae) is one of Curcuma spp. used as stomachic,carminative, haematic and styptic medicines. Dried rhizomes of C. zedoaria containessential oil and curcuminoids. Induced mutation of this species would provide andhelp plant breeders to improve and select better quality of the plant. The presentstudy was carried out to determine LD-50% of gamma irradiation of C. zedoaria, toinduce genetic variation using gamma irradiation, and to assess genetic diversityamong mutant genotypes using random amplified polymorphic DNA (RAPD)markers. The LD-50% of gamma irradiation was achieved at 813.32 rad. Chlorophyllmutations were recorded at vM1 and vM2. Result of genetic analysis showed thatRAPD profiles were different from that of the control, and genetic dissimilaritiesamong mutant genotypes were higher than that of the control.
PENAMPILAN GENOTIPE SOM JAWA {Talinumpaniculatum Jacq. (Gaertn.)} PADA GERERASI M2 Yuyu Suryasari Poerba
BERITA BIOLOGI Vol 7, No 3 (2004)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v7i3.1062

Abstract

Javasom [Talinum paniculatum Jacq. (Gaertn.] is one of popular vegetable plants with potential medicinal properties.The plant root is often used as a substitute for ginseng (Panax ginseng L.), and the leaves are used as a vegetable and as a substitute for purslane (Portulaca olaraeae L.).An effort to improve genetic quality of the plant was made through induced mutation with ethyl methane sulphonate (EMS).A variety of EMS dosages (0, 0.3%, 0.6%, 0.9%, 1.2%, 1.5% and 1.8%) were applied to javasom seeds to induce mutation for 24 hours at room temperature. A population of the first and second generation after mutagen treatment (M, and M2,respectively) was established and observed at vegetative and generative stages. At M, generation, plant growth was suppressed and plant yield was decreased.Chimeras and chlorophyll mutation were observed in every EMS-treatment level, which was indicated that mutation was induced in the plant. Lethal-dosage (LD-50) of EMS was at 1.2%-1.5%. M, population were recovered and showed variation in all parameters observed. High genetic variation coefficients were found in most of plant characters observed.All parameters have a medium to high heritability, which indicated that all parameter observed were relatively easily inherited.However, all genotypes are subjected to be evaluated in the next generation in their performance stability.
TOLERANSI BEBERAPA GENOTIPE Vigna umbellata (THUMB.) TERHADAP SUHU TINGGI PADA BERBAGAI TAHAP PERTUMBUHAN Yuyu Suryasari Poerba; Fauzia Syarif
BERITA BIOLOGI Vol 4, No 5 (1999)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v4i5.1243

Abstract

Degree of electrolyte leakage (EL) from leaf tissue after exposure to high temperature has been used as an indicator of heat tolerance. Inthe present study, EL was measured in an attempt to estimate heat tolerance of Viana umbellata (Thumb.) genotypes at three different growth phases. The degree of heat injury is significantly different among the three growth phases and among the genotypes as well as their interaction. However, the degree of heat injury tends to increase over the plant growth. The 18 genotypes of V. umbellata shows moderate genetic variability as shown by its value of genetic variance (46,25 ±16,35), phenotypic variance (85,37 ±27,60), and coefficient of genetic variation (26,36%).
Identifikasi Tingkat Ploidi Tanaman Pisang Menggunakan Flow Cytometer di Jawa Tengah Herlina; Yuyu Suryasari Poerba; Tri Handayani; Fajarudin Ahmad; Diyah Martanti
MANILKARA: Journal of Bioscience Vol 2 No 2 (2024): Februari
Publisher : LPPM Universitas Terbuka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33830/manilkara.v2i2.7573.2024

Abstract

In Indonesia, banana plants are known to have high genetic diversity, both cultivated and wild bananas. Banana plants are naturally known to have several levels of ploidy, namely diploid, triploid, and tetraploid. This study aims to identify the level of ploidy in banana plants explored in Central Java. The research method used flow cytometer, which analyzes the size of the plant genome, thus providing an overview of the level of ploidy and genetic diversity in banana plant populations. The research samples were banana plant leaves from various exploration locations in Central Java. The identification results show that of the 30 banana plant leaf samples that were successfully analyzed, it is known that the largest number of samples from banana plants that have triploid ploidy level (2n = 3x), namely 15 numbers, diploid ploidy level (2n = 2x) as many as 14 numbers, and found 1 cultivated banana with tetraploid ploidy level (2n = 4x). The results of ploidy level identification can provide guidance for researchers and plant breeders in selecting the right genotype for the development of superior varieties.
Co-Authors Diah Martanti Diyah Martanti Elizabeth A. Widjaya Fajarudin Ahmad FAUZIA SYARIF Herlina Tri Handayani