Article Per Year (5 Year)
p-Index From 2020 - 2025
0.23
P-Index
This Author published in this journals
All Journal Jurnal Ilmu Kedokteran (Journal of Medical Science) Althea Medical Journal Budapest International Research and Critics Institute-Journal (BIRCI-Journal): Humanities and Social Sciences
Sunarjati Sudigdoadi
Unknown Affiliation
Religion Arts Humanities Biochemistry, Genetics & Molecular Biology Dentistry Economics, Econometrics & Finance Health Professions Medicine & Pharmacology Public Health Social Sciences

Published : 11 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 1 Documents
Search
Journal : Jurnal Ilmu Kedokteran (Journal of Medical Science)

 1 
Detection of Staphylococcus aureus’s Strain Similarity on Surgical Ward Nurses’s Hand and Nose and Post Operative Wound Infection Using Coa Gene Through PCR-RFLP Method Fauzia Andrini; Imam Supardi; Sunarjati Sudigdoadi; Sadeli Masria
Jurnal Ilmu Kedokteran Vol 4, No 2 (2010): Jurnal Ilmu Kedokteran
Publisher : Fakultas Kedokteran Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (212.126 KB) | DOI: 10.26891/JIK.v4i2.2010.116-122

Abstract

Staphylococcus aureus (S.aureus) remains to be the most important cause of post operative wound infection. Nursescould become reservoirs to transmit S.aureus through contaminated hands transiently, or through colonized nose.Strain polymorphism could be determined by Restriction Fragment Length Polymorphism (RFLP), using coa gene andrestriction endonuclease enzyme Alu1. There were 30 isolates of S.aureus’s infection, and 20 isolates taken from handsand nose of the nurses in charge. From 50 isolate positive S.aureus, PCR results showed single and multiple bandswithin 300 to 900 base pairs (bp) in length, and multiple bands within 200 to 600 bp. Five out of 30 patients (17%)showed no PCR-RFLP similarity with any of the nurses. Ten out of 15 nurses which hands were positive for S.aureus,has PCR-RFLP similarity with some patients. There was only 1 out of 5 nurses which nose was positive for S.aureus,showed PCR-RFLP similarity with some patients. Statistically, the proportion of the similar PCR-RFLP between thosesamples in this study is 0.12 (12%). Conclusion: Nurses had 12 % PCR-RFLP similarity for S.aureus with post operativewound infection.
Co-Authors Abdullah Firmansyah W. Andri Rezano Andriana Purnama Aris Primadi Fauzia Andrini Gaga Irawan Nugraha Gowre Govindasamy Hardisiswo Soedjana Imam Supardi Jeevanisha Patmanathan Julius B Dewanto Kavita Arumugam Mohd Hafeez bin Mohd Rafee Nadiyah Nadiyah Nadya Iswandari Ricky Adriansjah Sadeli Masria Sefti Atletika Asiani Syed Anwar Husain Theruna Huthamaputiran Usep Abdullah Husin Yanti Mulyana Yodya Evila Yoni Fuadah Syukriani