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Endang Setiani
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Detection of Pathogenic Leptospires in Rat and Shallow Populations and Its Spatial Distribution in Bakaran Kulon Village, Pati District Dyah Widiastuti; Nova Pramestuti; Zumrotus Sholichah; Endang Setiani; Raden Ludhang P Rizki
Insights in Public Health Journal Vol 2 No 1 (2021): Insights in Public Health Journal
Publisher : Public Health Department, Faculty of Health Sciences, Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.iphj.2021.2.1.4979

Abstract

Background In tropical areas, Leptospirosis is still considered a public health problem, particularly in the event of heavy rainfall and flooding. Transmission of leptospira bacteria from infected animals, such as rats, takes place through the urine or blood. Different serovar leptospires can be found in different reservoir animals in the field. However, research on the presence of pathogenic leptospires in rats in Pati Regency, one of the areas with leptospirosis cases in Central Java, is very limited. Objectives: The purpose of this research is to identify Leptospira characteristics. Spp in rat and its distribution in Pati Regency. Methods: Rat capture was carried out in Bakaran Kulon Village in Pati Regency. Detection of pathogenic leptospires was carried out using the ropB gene and followed by phylogenetic analysis. As additional details, a buffer analysis was carried out to measure the distance between the positions of cases of leptospira and positive rats positions. Results: Pathogenic were found in 11.76% (2/17) of rats and shallows in the area of study. Based on DNA leptospires sequencing, Leptospira found in Bakaran Kulon Village Pati Regency were Leptospira interrogans serovar Bataviae and Leptospira borgpetersenii serovar Ballum, both of which are pathogenic. Based on a buffer analysis, Leptospira-positive rat was found around cases of leptospirosis at distances of 30 and 60 m. Conclusion: This study shows that Leptospira spp. in rats found around the location of leptospirosis cases, which indicates that rats have great potential as transmitters of leptospirosis to humans.
The Antifungal Effect of WHO Hand Sanitizer to Candida Albicans in Improving Personal Hygiene Dyah Widiastuti; Ihda Zuyina Zuyina Ratna Sari; Isya Fikria Kalimah; Endang Setiani
Insights in Public Health Journal Vol 1 No 2 (2020): Insights in Public Health Journal
Publisher : Public Health Department, Faculty of Health Sciences, Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.iphj.2020.1.2.3734

Abstract

Hand hygiene is one of the important factors that determine a person's health status. Hands are easily contaminated by microbes from the environment such as bacteria, viruses, and fungi through direct contact. Hand sanitizer is a type of media that can be used to clean hands from disease-causing microbes other than soap. Hand sanitizers are widely used by the community because they are considered more practical to use. This study aims to determine the anti-fungal activity of the hand sanitizer recommended by the World Health Organization (WHO) against Candida albicans. Anti-fungal activity was tested using the well diffusion method. The results showed that the higher the concentration of the hand sanitizer, the bigger the inhibition zone formed. The statistical test results obtained a significance value of p <0.05 at the variation of the concentration of hand sanitizer 50%, 75%, 100%, and treatment control. These results indicate that there is a significant difference between the hand sanitizer treatment and control of the inhibition zone formation in C. albicans. The WHO recommended hand sanitizer has medium inhibitory power against C. albicans bacteria.
The Antifungal Effect of WHO Hand Sanitizer to Candida Albicans in Improving Personal Hygiene Dyah Widiastuti; Ihda Zuyina Zuyina Ratna Sari; Isya Fikria Kalimah; Endang Setiani
Insights in Public Health Journal Vol 1 No 2 (2020): Insights in Public Health Journal
Publisher : Public Health Department, Faculty of Health Sciences, Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.iphj.2020.1.2.3734

Abstract

Hand hygiene is one of the important factors that determine a person's health status. Hands are easily contaminated by microbes from the environment such as bacteria, viruses, and fungi through direct contact. Hand sanitizer is a type of media that can be used to clean hands from disease-causing microbes other than soap. Hand sanitizers are widely used by the community because they are considered more practical to use. This study aims to determine the anti-fungal activity of the hand sanitizer recommended by the World Health Organization (WHO) against Candida albicans. Anti-fungal activity was tested using the well diffusion method. The results showed that the higher the concentration of the hand sanitizer, the bigger the inhibition zone formed. The statistical test results obtained a significance value of p <0.05 at the variation of the concentration of hand sanitizer 50%, 75%, 100%, and treatment control. These results indicate that there is a significant difference between the hand sanitizer treatment and control of the inhibition zone formation in C. albicans. The WHO recommended hand sanitizer has medium inhibitory power against C. albicans bacteria.
Detection of Pathogenic Leptospires in Rat and Shallow Populations and Its Spatial Distribution in Bakaran Kulon Village, Pati District Dyah Widiastuti; Nova Pramestuti; Zumrotus Sholichah; Endang Setiani; Raden Ludhang P Rizki
Insights in Public Health Journal Vol 2 No 1 (2021): Insights in Public Health Journal
Publisher : Public Health Department, Faculty of Health Sciences, Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.iphj.2021.2.1.4979

Abstract

Background In tropical areas, Leptospirosis is still considered a public health problem, particularly in the event of heavy rainfall and flooding. Transmission of leptospira bacteria from infected animals, such as rats, takes place through the urine or blood. Different serovar leptospires can be found in different reservoir animals in the field. However, research on the presence of pathogenic leptospires in rats in Pati Regency, one of the areas with leptospirosis cases in Central Java, is very limited. Objectives: The purpose of this research is to identify Leptospira characteristics. Spp in rat and its distribution in Pati Regency. Methods: Rat capture was carried out in Bakaran Kulon Village in Pati Regency. Detection of pathogenic leptospires was carried out using the ropB gene and followed by phylogenetic analysis. As additional details, a buffer analysis was carried out to measure the distance between the positions of cases of leptospira and positive rats positions. Results: Pathogenic were found in 11.76% (2/17) of rats and shallows in the area of study. Based on DNA leptospires sequencing, Leptospira found in Bakaran Kulon Village Pati Regency were Leptospira interrogans serovar Bataviae and Leptospira borgpetersenii serovar Ballum, both of which are pathogenic. Based on a buffer analysis, Leptospira-positive rat was found around cases of leptospirosis at distances of 30 and 60 m. Conclusion: This study shows that Leptospira spp. in rats found around the location of leptospirosis cases, which indicates that rats have great potential as transmitters of leptospirosis to humans.
Co-Authors Dyah Widiastuti Dyah Widiastuti Ihda Zuyina Zuyina Ratna Sari Isya Fikria Kalimah Nova Pramestuti Raden Ludhang P Rizki Zumrotus Sholichah