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Dyah Widiastuti
Balai Litbang Banjarnegara
Agriculture, Biological Sciences & Forestry Health Professions Medicine & Pharmacology Public Health

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The Antifungal Effect of WHO Hand Sanitizer to Candida Albicans in Improving Personal Hygiene Dyah Widiastuti; Ihda Zuyina Zuyina Ratna Sari; Isya Fikria Kalimah; Endang Setiani
Insights in Public Health Journal Vol 1 No 2 (2020): Insights in Public Health Journal
Publisher : Public Health Department, Faculty of Health Sciences, Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.iphj.2020.1.2.3734

Abstract

Hand hygiene is one of the important factors that determine a person's health status. Hands are easily contaminated by microbes from the environment such as bacteria, viruses, and fungi through direct contact. Hand sanitizer is a type of media that can be used to clean hands from disease-causing microbes other than soap. Hand sanitizers are widely used by the community because they are considered more practical to use. This study aims to determine the anti-fungal activity of the hand sanitizer recommended by the World Health Organization (WHO) against Candida albicans. Anti-fungal activity was tested using the well diffusion method. The results showed that the higher the concentration of the hand sanitizer, the bigger the inhibition zone formed. The statistical test results obtained a significance value of p <0.05 at the variation of the concentration of hand sanitizer 50%, 75%, 100%, and treatment control. These results indicate that there is a significant difference between the hand sanitizer treatment and control of the inhibition zone formation in C. albicans. The WHO recommended hand sanitizer has medium inhibitory power against C. albicans bacteria.
Aktivitas enzim monooksigenase pada populasi nyamuk Aedes aegypti di Kecamatan Tembalang, Kota Semarang Dyah Widiastuti; Sunaryo Sunaryo; Nova Pramestuti; Martini Martini
ASPIRATOR - Journal of Vector-borne Disease Studies Vol 7 No 1 (2015): Jurnal Aspirator Volume 7 Nomor 1 2015
Publisher : Loka Litbang Kesehatan Pangandaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (638.483 KB)

Abstract

Dengue Haemorrhagic Fever (DHF) is a major health problem in Tembalang sub district, Semarang City. Fogging with insecticide applications was done frequently as an effort to control Dengue vectors. The use of insecticides from the same class in a long time can lead to resistance in mosquitos’ population. The research aimed to observe the activity of monooxygenases in Aedes aegypti populations in Tembalang Subdistrict, Semarang. The study was conducted during February-November 2014 with a cross-sectional design in 10 villages in Tembalang Subdistirict, Semarang City. Field strains of Ae. aegypti eggs were collected using ovitraps. The collected eggs were grown under standard condition to adult mosquitoes. Mosquitos’ homogenate were stored at -85°C and used for biochemical assays. The results showed there was increased monooxygenases activity in Ae. aegypti populations. Resistance to synthetic pyrethroid insecticide in Ae. aegypti mosquitoes population in Tembalang Subdistrict might be caused by the mechanism of detoxification enzymes in particular monooxygenases
The Antifungal Effect of WHO Hand Sanitizer to Candida Albicans in Improving Personal Hygiene Dyah Widiastuti; Ihda Zuyina Zuyina Ratna Sari; Isya Fikria Kalimah; Endang Setiani
Insights in Public Health Journal Vol 1 No 2 (2020): Insights in Public Health Journal
Publisher : Public Health Department, Faculty of Health Sciences, Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.iphj.2020.1.2.3734

Abstract

Hand hygiene is one of the important factors that determine a person's health status. Hands are easily contaminated by microbes from the environment such as bacteria, viruses, and fungi through direct contact. Hand sanitizer is a type of media that can be used to clean hands from disease-causing microbes other than soap. Hand sanitizers are widely used by the community because they are considered more practical to use. This study aims to determine the anti-fungal activity of the hand sanitizer recommended by the World Health Organization (WHO) against Candida albicans. Anti-fungal activity was tested using the well diffusion method. The results showed that the higher the concentration of the hand sanitizer, the bigger the inhibition zone formed. The statistical test results obtained a significance value of p <0.05 at the variation of the concentration of hand sanitizer 50%, 75%, 100%, and treatment control. These results indicate that there is a significant difference between the hand sanitizer treatment and control of the inhibition zone formation in C. albicans. The WHO recommended hand sanitizer has medium inhibitory power against C. albicans bacteria.
Co-Authors Endang Setiani Ihda Zuyina Zuyina Ratna Sari Isya Fikria Kalimah Martini Martini Nova Pramestuti Sunaryo Sunaryo