Wahdah Norsiah
Medical Laboratory Technology Department, Poltekkes Kemenkes Banjarmasin, Indonesia

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Pediculosis Capitis dan Personal Hygiene pada Anak SD di Daerah Pedesaan Kotamadya Banjarbaru Rifqoh Mukhtar Mayasin; Wahdah Norsiah
Medical Laboratory Technology Journal Vol. 3 No. 2 (2017): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (920.248 KB) | DOI: 10.31964/mltj.v3i2.134

Abstract

Pediculosis capitis or head lice infestation caused by Pediculus humanus var. capitis. Its caused irritation and scalp pruritus. Its occurs most commonly in children becaused they couldn’t keep their personal hygiene independently. Pediculosis capitis caused disconcentrated, lack of sleep, parental anxiety, social embarrassment and isolation. The objective of this study was to determine the correlation between Pediculosis capitis with personal hygiene in rural primary schoolchildren in Cempaka Village, Banjarbaru Municipality. This analytic observational case control study design, at first determined 33 (19,87%) schoolchildren was infested pediculosis capitis from 166 population. Other 33 schoolchildren randomized uninfested pediculosis capitis as the controls group (1:1 to case group). Pediculosis capitis was known by identified head lice, nymph or eggs of Pediculus humanus var. capitis and personal hygiene was measured by questionnaire. Chi Square test result, there was significant moderate positive correlation between pediculosis capitis with personal hygiene (p value
Evaluation of the Diagnosis of Typhoid Fever Using the Widal Test and the Anti Salmonella typhi IgM Test Wahdah Norsiah; Neni Oktiyani
Medical Laboratory Technology Journal Vol. 6 No. 2 (2020): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (266.856 KB) | DOI: 10.31964/mltj.v6i2.327

Abstract

Typhoid fever is a systemic infectious disease caused by Salmonella enteric serotype typhi. Laboratory tests are essential because the clinical symptoms of typhoid fever are not specific. The definitive diagnosis of typhoid fever make by culture isolation of Salmonella typhi, but this test is expensive and takes a long time. Other supporting tests that can do quickly are the Widal and Salmonella typhi IgM tests. This study aimed to determine the suitability between the results of the Widal test and the results of the anti-Salmonella typhi IgM test. This type of research is an observational analytic study determining the sample based on the accidental sampling technique. The number of samples taken was 42 samples. The results showed that the Widal Test in typhoid patients was 76% positive and 24% negative. Based on the anti-Salmonella typhi IgM test results in typhoid patients, 38% were positive, and 62% were negative. The study concluded a fragile agreement between the Widal test results and the results of the anti-Salmonella typhi IgM test. It recommended that the Widal test carried out for the initial examination and a quick community survey. The Tubex test highly recommends for diagnosis.
The Potential at Phytochemical Compounds from the Indonesian Kalimantan Plant as Covid-19 Immunomodulators Chairana Fadya; Ahmad Luthfi Haqiqi; Annisa Miladdiannur; Monika Yati; Ummu Salamah Az Zahra; Neni Oktiyani; Wahdah Norsiah
Tropical Health and Medical Research Vol. 4 No. 1 (2022): Tropical Health and Medical Research
Publisher : Baiman Bauntung Batuah Center

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35916/thmr.v4i1.60

Abstract

Herbal plants are alternative medicine and immunity boosters related to the Covid-19 pandemic. Typical Kalimantan plants that have the potential are dayak onion (Eleutherine americana Merr.), karamunting (Rhodomyrtus tematosa (Aiton) Hassk), pasak bumi (Eurycoma longifolia Jack), and tigaron (Crataeva adansonii DC). This study aims to determine the potential of phytochemical compounds from Indonesian Kalimantan plants as Covid-19 immunomodulators. Descriptive research with cross-sectional study design by testing the typical plants of Kalimantan, which were extracted first through the maceration method and then carried out qualitative and quantitative phytochemical tests. Qualitative and quantitative results showed that the four positive plants contained phytochemical alkaloids, flavonoids, saponins, and tannins in certain levels. Quantitative results on dayak onion (Eleutherine americana Merr.) obtained alkaloid content; flavonoids; saponins; tannins were 74.540%; 22.125 mg/mL; 58.350%; 0.342 mg/mL. karamunting fruit (Rhodomyrtus tematosa (Aiton) Hassk) 72.815%; 20,500 mg/mL; 78.225%; 0.541 mg/mL. pasak bumi (Eurycoma longifolia Jack) 68.575%; 12,250 mg/mL; 53.930%; 0.383 mg/mL; and tigaron (Crataeva adansonii DC) 82.330%; 40,750 mg/mL; 75.395%; 0.609 mg/mL. The four phytochemical compounds have the potential as Covid-19 immunomodulators. This study concludes that the typical plant from Kalimantan that has been studied has the potential. This research can be continued by testing the effectiveness of phytochemicals as Covid-19 immunomodulators in experimental animals.
Utilization of Alternative Buffer Solutions for Staining Thin Blood smears by the Giemsa, Wright stain and Romanowsky method Neni Oktiyani; Ahmad Muhlisin; Erfan Roebiakto; Wahdah Norsiah; Mahpolah Mahpolah
Tropical Health and Medical Research Vol. 5 No. 1 (2023): Tropical Health and Medical Research
Publisher : Baiman Bauntung Batuah Center

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35916/thmr.v4i1.76

Abstract

To make a reliable diagnosis on haematological examination, it is necessary to examine further the morphology of the blood cells previously stained in the procedure for staining thin blood smears using a buffer solution with a standard pH of 6.4 to 6.8. One of the problems that may occur in the laboratory is that the buffer reagents are damaged, past the expiration date or running out, so alternative buffers are needed that are cheap, fast and easy to obtain. This study aims to evaluate the staining results from alternative buffers. Study used a quasi-experimental method, and using alternative buffers from bottled mineral water following SNI-01-0553 2006. The colour produced by some of these alternative buffers is almost equivalent to blood cells stained with phosphate buffer. The percentage of assessment results in the alternative buffer codes B, C, D, E and F compared with control (A) were 62.67%, 92.00%, 82.67%, 80.00% 88, 00%, and 68.00%. The use of alternative buffers for staining thin blood smears using the Giemsa, Wright stain, and Romanowsky method can be done with mineral water as an alternative buffer for sample codes C, D, E and F, while B and G cannot be used. This alternative buffer can be applied by laboratory personnel in urgent situations in limited equipment and material facilities.
Hubungan Konsumsi Harian Rokok Terhadap Antibodi IgG S1RBD Pasca Vaksinasi COVID-19 pada Civitas Akademik Amalia Putri; Wahdah Norsiah; Tini Elyn Herlina; H. Haitami; Dinna Rakhmina
Jurnal Karya Generasi Sehat Vol. 1 No. 1 (2023): Edisi Desember Tahun 2023
Publisher : Poltekkes Kemenkes Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/jkgs.v1i1.29

Abstract

Vaksinasi dinilai sebagai cara paling efektif untuk penanganan pandemi akibat COVID-19. Vaksinasi memiliki efek yang berbeda pada tiap individu. Kandungan nikotin dalam rokok diduga menghambat aktivasi kekebalan bawaan dan adaptif, sehingga mempengaruhi pembentukan antibodi yang diinduksi dari vaksinasi.Tujuan penelitian ini untuk mengetahui ada atau tidaknya hubungan konsumsi harian rokok seseorang dengan kadar antibodi IgG S1RBD. Jenis penelitian yang digunakan adalah survei analitik dengan metode cross sectional study pada civitas akademik yang memiliki kebiasaan merokok. Hasil penelitian dari 28 responden didapatkan kadar rata-rata antibodi IgG S1RBD pada responden dengan konsumsi harian rokok 1-10 batang/ hari sebesar 255,59 IU/mL, 11-20 batang/ hari sebesar 173,15 IU/mL, lebih dari 20 batang/hari sebesar 143,79 IU/mL, dan pada non perokok sebesar 279,21 IU/mL. Uji statistik menunjukkan nilai signifikansi 0,000 sehingga disimpulkan semakin tinggi konsumsi harian rokok seseorang maka semakin menurun antibodi IgG S1RBD dalam tubuhnya.