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Ketepatan Hasil dan Variasi Waktu Pendidihan Pemeriksaan Zat Organik Haitami Haitami; Dinna Rakhmina; Syahid Fakhridani
Medical Laboratory Technology Journal Vol. 2 No. 2 (2016): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (657.144 KB) | DOI: 10.31964/mltj.v2i2.117

Abstract

The principle of organic substances determination in water samples is oxidized by an excess amount of KMnO4, then it is reduced excess amount of oxalic acid, the excess of oxalic acid is titrated back with KMnO4. Based on the procedures in ISO 06-6989.22-2004, a length of boiling time for organic substances determination in water samples is for 10 minutes. This study aimed to determine the effect of boiling time length to the accuracy of the organic substances determination result in water samples. This study was true experiment study with comparative study design. Samples were organic substances, specifically oxalic acid in distilled water in a concentration of 54 mg/L, which was examined for the organic substances level with a boiling time of 5, 10 and 15 minutes. The result on each boiling time length consecutively were 52,8 mg/L; 54,5 mg/L; dan 55,1 mg/L. The conclusion of the study was there was effect of boiling time length to the accuracy of organic substances determination result in water samples with the significance value of 0.002 (
Daya Hambat NaCl terhadap Pertumbuhan Staphylococcus aureus Amalia Amalia; Ratih Dewi Dwiyanti; Haitami Haitami
Medical Laboratory Technology Journal Vol. 2 No. 2 (2016): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (794.567 KB) | DOI: 10.31964/mltj.v2i2.125

Abstract

Staphylococcus aureus is a pathogenic bacterial that causes infectious disease and human poisoning through enterotoxin produced by the bacteria. Salt or sodium chloride is used by human for food preservation process because it can inhibit the growth of bacteria, especially Staphylococcus aureus. The purposes of the study were to determine the effect of NaCl in media on the growth of Staphylococcus aureus and determine the effective concentration of NaCl. The type of research used in this study was true experiment posttest only control group design. Samples were prepared in 5 NaCl concentrations, they were 10%, 15%, 20%, 25% dan 30%. The study conclusion was there were effects of NaCl addition to the media on the growth of Staphylococcus aureus with a significance value of 0.000 (
Lactic Acid Levels Yogurt Red Beans with Addition of Honey Trigona sp Ruri Widyati; Neni Oktiyani; Haitami Haitami
Tropical Health and Medical Research Vol. 1 No. 1 (2019): Tropical Health and Medical Research
Publisher : Baiman Bauntung Batuah Center

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (754.872 KB) | DOI: 10.35916/thmr.v1i1.4

Abstract

Red beans (Phaseolus vulgaris L.) are grains that are rich in nutrients and useful as a substitute for vegetable milk which can be processed into yogurt using Streptococcus thermophillus and Lactobacillus bulgaricus. Carbohydrates in red beans consist of a group of oligosaccharides that have little ability as an energy source for bacteria to produce lactic acid. Therefore, the making of red bean yogurt needs another source of sugar by adding Trigona sp. Honey. The purpose of this study was to determine the effect of adding Trigona sp honey by 0%, 1.9%, 3.8%, 5.7%, 7.6%, 9.5% to the total level of lactic acid in red bean yogurt incubated for 24 hours at 37oC. This type of research is an experiment with the Postest Only With Control Group Design research design using the alkalimetry titration method. The sampling technique used was purposive sampling technique, namely Trigona sp honey bee taken at Trigona sp bee farm in Tambangan, Tanah Laut. Indonesia. The results showed that there was an effect of adding Trigona sp honey to total lactic acid levels in red bean yogurt with the addition of Trigona sp honey 1.9% - 9.5% fulfilling the requirements of SNI 01.2981-2009. The highest average value of total lactic acid in red bean yogurt with the addition of Trigona sp honey 9.5% which is equal to 0.6672%. It is recommended for future researchers to use other types of carbohydrate sources and additives in the form of proteins. For the community, it is recommended to use alternative sources of other sugar and optimize the addition of Trigona sp. Honey.
Evaluation in Hematology and BCR-ABL Molecular Profiles in Patients with Chronic Myeloid Leukemia Undergoing Tyrosine Kinase Inhibitor Therapy Muhammad Ihza Lisan Shidqi; Budi Santosa; Muhamad Muslim; Haitami Haitami
Medical Laboratory Technology Journal Vol. 8 No. 2 (2022): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (284.462 KB) | DOI: 10.31964/mltj.v8i1.484

Abstract

Chronic myeloid leukemia (CML) is a myeloproliferative malignancy due to the formation of the BCR-ABL fusion gene in chronic myeloid leukemia. This condition causes excessive cell proliferation, resulting in an increase in the number of leukocytes. Tyrosine Kinase Inhibitor (TKI) is a first-line therapy that helps reduce the percentage of the Breakpoint Cluster Region–Abelson (BCR-ABL) fusion gene in patients with chronic myeloid leukemia. This study was conducted to determine evaluation in the hematological profiles (hemoglobin levels, leukocyte counts, platelet counts) and molecular BCR-ABL in patients with chronic myeloid leukemia before and after 12 months of tyrosine kinase inhibitors therapy. This analytic observational study was administered using a cross-sectional design to in analyzing the medical records of CML patients who underwent TKI therapy at the Sub Specialist Polyclinic of Internal Medicine Hematology Oncology Ulin Banjarmasin Indonesia Regional Hospital from March 2021-April 2022. Statistical test was performed which analysis results showed that 12-month tyrosine kinase inhibitor therapy could increase the hemoglobin levels, decrease leukocyte counts, platelet counts as well as decreasing the percentage of BCR-ABL gene fusion in patients with chronic myeloid leukemia. In conclusion, evaluation of tyrosine kinase inhibitor therapy in patients with chronic myeloid leukemia obtained significant differences in the hematological profiles and the molecular BCR-ABL. Further researchers are recommended to compare the type of tyrosine kinase inhibitor therapy between Imatinib and Nilotinib on the hematological and molecular profiles of BCR-ABL in patients with chronic myeloid leukemia with a larger sample count.
Hubungan Konsumsi Harian Rokok Terhadap Antibodi IgG S1RBD Pasca Vaksinasi COVID-19 pada Civitas Akademik Amalia Putri; Wahdah Norsiah; Tini Elyn Herlina; H. Haitami; Dinna Rakhmina
Jurnal Karya Generasi Sehat Vol. 1 No. 1 (2023): Edisi Desember Tahun 2023
Publisher : Poltekkes Kemenkes Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/jkgs.v1i1.29

Abstract

Vaksinasi dinilai sebagai cara paling efektif untuk penanganan pandemi akibat COVID-19. Vaksinasi memiliki efek yang berbeda pada tiap individu. Kandungan nikotin dalam rokok diduga menghambat aktivasi kekebalan bawaan dan adaptif, sehingga mempengaruhi pembentukan antibodi yang diinduksi dari vaksinasi.Tujuan penelitian ini untuk mengetahui ada atau tidaknya hubungan konsumsi harian rokok seseorang dengan kadar antibodi IgG S1RBD. Jenis penelitian yang digunakan adalah survei analitik dengan metode cross sectional study pada civitas akademik yang memiliki kebiasaan merokok. Hasil penelitian dari 28 responden didapatkan kadar rata-rata antibodi IgG S1RBD pada responden dengan konsumsi harian rokok 1-10 batang/ hari sebesar 255,59 IU/mL, 11-20 batang/ hari sebesar 173,15 IU/mL, lebih dari 20 batang/hari sebesar 143,79 IU/mL, dan pada non perokok sebesar 279,21 IU/mL. Uji statistik menunjukkan nilai signifikansi 0,000 sehingga disimpulkan semakin tinggi konsumsi harian rokok seseorang maka semakin menurun antibodi IgG S1RBD dalam tubuhnya.