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All Journal Jurnal Primatologi Indonesia Forum Pasca Sarjana Jurnal Kesehatan Masyarakat (JKM) CENDEKIA UTAMA
Sulistiyani .
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Education Medicine & Pharmacology Public Health

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Respon Ekspresi Molekul Adhesi pada Kultur Sel Endotel yang diinduksi LDL Teroksidasi dari Macaca fascicularis terhadap Ekstrak Kurkuminoid Temumangga Trini Susmiati; Sulistiyani .; Dondin Sajuthi; Latifah K. Darusman
Jurnal Primatologi Indonesia Vol. 6 No. 2 (2009)
Publisher : Pusat Studi Satwa Primata LPPM-IPB

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Abstract

Curcuminoids of Curcuma manggo believed to have antioxidant properties that can prevent the occurrence of atherosclerosis.  However, the mechanism at the molecular level is not clearly known. Increase in intracellular adhesion molecule-1 (ICAM-1) will result in endothelial cell dysfunction that initiates athersclerosis. The expression of ICAM-1 can be triggered by oxidized LDL molecules. The purpose of this study is to elucidate the effects of curcuminoids temumangga (Curcuma mango) on the expression of ICAM-1 induction in endothelial cell culture. Prior to the induction of the expression of ICAM-1, endothelial cell culture was pre-incubated with curcuminoids extract for 48 hours with concentration of 2 ppm and 8 ppm, then further incubated with oxidized LDL for 24 hours. The treated cells were compared to 5 µM Cu2+ induced endothelial cell culture with or without LDL  from Macaca fascicularis.  ICAM-1 expression was determined by immunohistochemistry using anti-ICAM-1 antibodies and visualized with staining by 3.3-diaminobenzidin tetrahidrochloride (DAB). Results showed that surface-induced endothelial cell culture with ion Cu2+ stained well by DAB as brown yellow, which indicates ICAM-1 expression. Curcuminoids extract of  8 ppm reduced induction of ICAM-1 response. Induction of ICAM-1 expression by oxidized LDL caused foam cell accumulation in addition to ICAM-1 expression.Key words: Curcuminoid, low density lipoprotein (LDL), Macaca fascicularis, intracellular adhesion molecule-1 (ICAM-1), immunohistochemistry
The oxidation of low-density lipoprotein (LDL) is believed to be the initiating factor for the development and progression of atherosclerosis.  Curcuminoid, the metabolite of Zingiberaceae family such as temu mangga (Curcuma mangga), has been shown to reduce the susceptibility of LDL to oxidation. In this study, we examined the effect of curcuminoid extracted from temu mangga on copper ion-induced lipid peroxidation of low density lipoprotein (LDL) in mice’s macrophages and Macaca nemestrina’s m Trini i Susmiat; Sulistiyani .; Dondin Sajuthi; Latifah K. Darusman
Forum Pasca Sarjana Vol. 33 No. 1 (2010): Forum Pascasarjana
Publisher : Forum Pasca Sarjana

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The oxidation of low-density lipoprotein (LDL) is believed to be the initiating factor for the development and progression of atherosclerosis.  Curcuminoid, the metabolite of Zingiberaceae family such as temu mangga (Curcuma mangga), has been shown to reduce the susceptibility of LDL to oxidation. In this study, we examined the effect of curcuminoid extracted from temu mangga on copper ion-induced lipid peroxidation of low density lipoprotein (LDL) in mice’s macrophages and Macaca nemestrina’s monocytes.  Analyses were done by measuring the formation of thiobarbituric acid reactive substance (TBARS) as malonaldehyde (MDA).  LDL were harvested and isolated from 5 adult female Macaca fascicularis  fed aterogenic diet for 3 months.  LDL oxidation by mice macrophage incubated for 4 hours were inhibited by curcuminoid at concentration of 8 ppm.  There was decreased 17% (P<0.01) in the concentration of MDA compared to control without curcuminoid (31.99B±0. vs 36.77A±0.9 nmol/mg protein LDL, respectively).  Inhibiton of LDL oxidation in M. nemestrina macrophage were highest by curcuminoid at 8 ppm for 4 hours and 6 hours incubation.  There was 14.8% and 23% inhibiton (P<0.01) (23.768 ± 0.095A vs 27.111B±0.972 and 23.37B±0.12 vs 30.87A±2.49 nmol/mg LDL protein, respectively).  These data suggest that curcuminoid of temu mangga were able to inhibit LDL oxidation in the cellular level, therefore offer protection against oxidation of LDL.   Key words: LDL, atherosclerosis, curcuminoid, Curcuma mangga
EVALUASI PELAKSANAAN PELAYANAN CAKUPAN K4 DI PUSKESMAS JAKENAN PATI Sulistiyani .; Ervi Rachma Dewi
JKM (Jurnal Kesehatan Masyarakat) Cendekia Utama Edisi Agustus 2016
Publisher : STIKES Cendekia Utama Kudus

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (882.338 KB) | DOI: 10.31596/jkm.v0i0.119

Abstract

Pelayanan cakupan K4 merupakan cakupan pelayanan antenatal secara lengkap dengan memenuhi standar pelayanan. Jika pelayanan cakupan K4 tidak terpenuhi sesuai standar dapat menyebabkan bahaya bagi ibu hamil dan  bayinya. Presentase pelayanan cakupan K4 di Puskesmas Jakenan belum mencapai target SPM dari tahun 2013-2015. Tujuan penelitian ini adalah untuk mengevaluasi pelaksanaan pelayanan cakupan K4 di Puskesmas Jakenan dari sisi input, proses dan output. Jenis penelitian deskriptif kualitatif. Data dikumpulkan dengan wawancara mendalam menggunakan daftar pertanyaan. Pada input pelayanan cakupan K4 di Puskesmas Jakenan, SDM masih kurang, sarana-prasarana lengkap dan memadai, adanya dukungan dana dalam pelaksanaan pelayanan cakupan K4, serta adanya SOP atau kebijakan pelayanan cakupan K4. Proses alur pelayanan cakupan K4 di Puskesmas Jakenan sudah sesuai dengan ketentuan tim akreditasi, namun dimodifikasi oleh pihak Puskesmas Jakenan sesuai kondisi Puskesmas. Pelaksanaan pelayanan cakupan K4 berdasarkan SOP pelayanan ibu hamil. Tidak tercapainya target pelayanan cakupan K4 dikarenakan  kurangnya sumber daya manusia, kesadaran ibu hamil kurang untuk melakukan pemeriksaan, terdapat ibu hamil yang tidak melakukan K1, dan ketidaktepatan prediksi dalam penentuan sasaran. Pelaksanaan pelayanan cakupan K4 masih mengalami kekurangan sumber daya manusia, kesadaran ibu hamil kurang untuk melakukan pemeriksaan, terjadinya kehilangan pemberian pelayanan K1, dan ketidaktepatan prediksi penentuan sasaran ibu hamil.Kata Kunci: cakupan K4, input, proses, output
Co-Authors Dondin Sajuthi Ervi Rachma Dewi Latifah K. Darusman Trini i Susmiat Trini Susmiati