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SUPLEMENTASI STEROL LEMBAGA GANDUM (Triticum sp.) PADA MARGARIN (Supplementation of Margarine with Wheat Germ Sterol) Sri Anna Marliyati; Hidayat Syarief; Deddy Muchtadi; Latifah K. Darusman; Rimbawan .
Jurnal Teknologi dan Industri Pangan Vol. 21 No. 1 (2010): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

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Margarine is a water in oil (w/o) emulsion product which is widely used for household cooking and baking industry. Consuming of margarine, which contains trans fatty acid may cause health problem due to the increase of LDL cholesterol. Since margarine is also a good carrier of phytosterol which prevent the absorption of cholesterol, there is a possibility to formulate a healthier margarine. In this research formulation and characteristics of products was investigated. The research work consisted of two steps: (1) supplementation of wheat germ sterol into margarine (two methods) and (2) analysis of physical, chemical characteristics and hedonic score. Parameters of physical characteristics were melting point and emulsion stability, whereas chemical characteristics were water and oil contents. The hedonic test was carried out based on product’s color, odor, taste, texture, and spreadability. Results showed that method II of supplementation produced better margarine than method I, in which the concentration of sterol in the margarine was higher with a melting point similar to that of control, better emulsion stability, and higher hedonic score. Supplementation process was carried out by mixing sterol into fat phase melted at 50 0C, followed by mixing with aqueous phase at 4 0C. Sterol used for method II was extracted using mixed solvent of hexane and ethanol at the ratio of 1:2 (v/v), which was resulted from previous experimentation.

MODEL OTENTIKASI KOMPOSISI OBAT BAHAN ALAM BERDASARKAN SPEKTRA INFRAMERAH DAN KOMPONEN UTAMA STUDI KASUS : OBAT BAHAN ALAM/FITOFARMAKA PENURUN TEKANAN DARAH Agus Mohamad Soleh; Latifah K. Darusman; Mohamad Rafi
FORUM STATISTIKA DAN KOMPUTASI Vol. 13 No. 1 (2008)
Publisher : FORUM STATISTIKA DAN KOMPUTASI

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Komposisi kimia yang terkandung dalam ekstrak obat bahan alam merupakan suatu komposisi yang kompleks, dengan demikian pengujian keotentikannya tidak dapat dilakukan melalui pedekatan tunggal. Salah satu teknik analisis yang dapat menggambarkan secara menyeluruh karakteristik kimia suatu bahan adalah teknik spektroskopi FTIR. Spektra FTIR dihasilkan dari interaksi antara energi sinar inframerah dan komponen kimia penyusun campuran bahan, sehingga suatu spektra FTIR merupakan indentitas khas campuran tersebut. Keotentikan komposisi suatu obat bahan alam pada studi ini ditentukan berdasarkan pada analisis komponen utama spektra inframerahnya. Studi dilakukan pada obat bahan alam/fitofarmaka penurun tekanan darah (Tensigard® : terdiri dari ekstrak seledri dan ekstrak daun kumis kucing). Pengukuran spektra inframerah dilakukan terhadap formula obat yang persentase komposisinya ditentukan melalui simplex lattice design. Selain itu pengukuran spektra inframerah juga dilakukan terhadap formula obat dengan mengganti (adulterasi) ekstrak kumis kucing dengan obat sintetis (reserpin) dan ekstrak sambiloto. Berdasarkan plot antara skor komponen utama pertama dan skor komponen utama kedua menunjukkan plot tersebut dapat digunakan untuk mendeteksi komposisi obat, tetapi tidak dapat mendeteksi adanya adulterasi komposisi oleh bahan lain. Kata Kunci : model otentikasi fitofarmaka, simplex lattice design, komponen utama, tensigard

Respon Ekspresi Molekul Adhesi pada Kultur Sel Endotel yang diinduksi LDL Teroksidasi dari Macaca fascicularis terhadap Ekstrak Kurkuminoid Temumangga Trini Susmiati; Sulistiyani .; Dondin Sajuthi; Latifah K. Darusman
Jurnal Primatologi Indonesia Vol. 6 No. 2 (2009)
Publisher : Pusat Studi Satwa Primata LPPM-IPB

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Curcuminoids of Curcuma manggo believed to have antioxidant properties that can prevent the occurrence of atherosclerosis. However, the mechanism at the molecular level is not clearly known. Increase in intracellular adhesion molecule-1 (ICAM-1) will result in endothelial cell dysfunction that initiates athersclerosis. The expression of ICAM-1 can be triggered by oxidized LDL molecules. The purpose of this study is to elucidate the effects of curcuminoids temumangga (Curcuma mango) on the expression of ICAM-1 induction in endothelial cell culture. Prior to the induction of the expression of ICAM-1, endothelial cell culture was pre-incubated with curcuminoids extract for 48 hours with concentration of 2 ppm and 8 ppm, then further incubated with oxidized LDL for 24 hours. The treated cells were compared to 5 µM Cu2+ induced endothelial cell culture with or without LDL from Macaca fascicularis. ICAM-1 expression was determined by immunohistochemistry using anti-ICAM-1 antibodies and visualized with staining by 3.3-diaminobenzidin tetrahidrochloride (DAB). Results showed that surface-induced endothelial cell culture with ion Cu2+ stained well by DAB as brown yellow, which indicates ICAM-1 expression. Curcuminoids extract of 8 ppm reduced induction of ICAM-1 response. Induction of ICAM-1 expression by oxidized LDL caused foam cell accumulation in addition to ICAM-1 expression.Key words: Curcuminoid, low density lipoprotein (LDL), Macaca fascicularis, intracellular adhesion molecule-1 (ICAM-1), immunohistochemistry

The oxidation of low-density lipoprotein (LDL) is believed to be the initiating factor for the development and progression of atherosclerosis. Curcuminoid, the metabolite of Zingiberaceae family such as temu mangga (Curcuma mangga), has been shown to reduce the susceptibility of LDL to oxidation. In this study, we examined the effect of curcuminoid extracted from temu mangga on copper ion-induced lipid peroxidation of low density lipoprotein (LDL) in mice’s macrophages and Macaca nemestrina’s m Trini i Susmiat; Sulistiyani .; Dondin Sajuthi; Latifah K. Darusman
Forum Pasca Sarjana Vol. 33 No. 1 (2010): Forum Pascasarjana
Publisher : Forum Pasca Sarjana

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The oxidation of low-density lipoprotein (LDL) is believed to be the initiating factor for the development and progression of atherosclerosis. Curcuminoid, the metabolite of Zingiberaceae family such as temu mangga (Curcuma mangga), has been shown to reduce the susceptibility of LDL to oxidation. In this study, we examined the effect of curcuminoid extracted from temu mangga on copper ion-induced lipid peroxidation of low density lipoprotein (LDL) in mice’s macrophages and Macaca nemestrina’s monocytes. Analyses were done by measuring the formation of thiobarbituric acid reactive substance (TBARS) as malonaldehyde (MDA). LDL were harvested and isolated from 5 adult female Macaca fascicularis fed aterogenic diet for 3 months. LDL oxidation by mice macrophage incubated for 4 hours were inhibited by curcuminoid at concentration of 8 ppm. There was decreased 17% (P<0.01) in the concentration of MDA compared to control without curcuminoid (31.99B±0. vs 36.77A±0.9 nmol/mg protein LDL, respectively). Inhibiton of LDL oxidation in M. nemestrina macrophage were highest by curcuminoid at 8 ppm for 4 hours and 6 hours incubation. There was 14.8% and 23% inhibiton (P<0.01) (23.768 ± 0.095A vs 27.111B±0.972 and 23.37B±0.12 vs 30.87A±2.49 nmol/mg LDL protein, respectively). These data suggest that curcuminoid of temu mangga were able to inhibit LDL oxidation in the cellular level, therefore offer protection against oxidation of LDL. Key words: LDL, atherosclerosis, curcuminoid, Curcuma mangga

A laboratory scale research to obtain environmentally safe effluent from Final Waste Disposal Site leachate in Galuga owned by Regional Government of Bogor City was conducted from July 2006 through to April 2007. The experiment of production tolerable threshold effluent was carried out by aerating the leachate in 4 different aeration rates (0, 10, 30 and 70 liters/minute). The research found that the most effective method to reduce pollutant was aerating at the rate of 70 liters/minutes. Howev Nurhasanah .; Latifah K. Darusman; Surjono Hadi Sutjahjo; Bibiana Widiati Lay
Forum Pasca Sarjana Vol. 34 No. 1 (2011): Forum Pascasarjana
Publisher : Forum Pasca Sarjana

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A laboratory scale research to obtain environmentally safe effluent from Final Waste Disposal Site leachate in Galuga owned by Regional Government of Bogor City was conducted from July 2006 through to April 2007. The experiment of production tolerable threshold effluent was carried out by aerating the leachate in 4 different aeration rates (0, 10, 30 and 70 liters/minute). The research found that the most effective method to reduce pollutant was aerating at the rate of 70 liters/minutes. However, only some of pollutant (Cu, Zn, Pb and E.coli) can be reduced until below tolerable threshold. Keywords: effluent, leachate, tolerable threshold

BIOLOGICAL ACTIVITIES AFFORDED BY THE EXTRACT FROM RARU BARK TO INHIBIT ACTION OF ALPHA- GLUCOSIDASE ENZYMES Gunawan Pasaribu; Wasrin Syafii; Latifah K. Darusman
Indonesian Journal of Forestry Research Vol 8, No 1 (2011): Journal of Forestry Research
Publisher : Secretariat of Agency for Standardization of Environment and Forestry Instruments

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/ijfr.2011.8.1.32-49

Raru (Shoreabalanocarpoides Sym) signifies one of the tree species that grows widespread in Sumatra Island. Its bark portion is commonly used by local villagers as additional ingredient mixed to nira (sugar palm juice). This addition is intended to make the juice more durable and also to enrich its taste after the juice is previously fermented to become traditional toddy beverage or the so-call “tuak”. Local villagers believe that raru bark can reduce the level of blood sugar. As the relevance, the research was conducted to confirm that the extract from raru bark could afford its biological activities to inhibit alpha-glucosidase enzyme through its characterization, quantification, and isolation of its boactive compound. The extraction was performed using two methods (i.e.reflux and maceration techniques). Result revealed that the bark extract obtained from both techniques contained polyphenol compounds: flavonoid, saponin and tannin. Further, raru-bark extract from the reflux and maceration techniques could inhibit the action of alpha glucosidase enzymes on carbohydrate substrate ( i.e. p -nitrophenil-α-D-glucopyranose), at respectively 90.67% and 97.33%. Meanwhile, the inhibition activities afforded by the patented drug as a control (i.e. glucobay) equaled to 97.05%. Assesment using UV-VIS spectroscopy, showed that the maximum spectrum of bioactive compound in the extract was at the wave length of 288.6 nm. Scrutiny using FTIR spectroscopy could identif y the presence of aromatic groups in the compound, containing -OH, C-H, C=C, C-O and C-H bond types. Analysis using GC-MS exhibited that the compound had molecular weight of 390 with molecular structure as C20H22O8. Ultimately, data analysis scrutiny with the aid of NMR judged the most plausible compound as bioactive was 4-Glucosyl-3, 4’, 5-trihydroxystilbene.

PENETAPAN BAHAN DIAGNOSIS STATUS HARA NPK PADA JARINGAN TANAMAN PEGAGAN Hermanto Hermanto; Munif Ghulamahdi; Latifah K. Darusman; Nurliani Bermawie; Atang Sutandi
Buletin Penelitian Tanaman Rempah dan Obat Vol 22, No 2 (2011): BULETIN PENELITIAN TANAMAN REMPAH DAN OBAT
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/bullittro.v22n2.2011.%p

Studi fisiologi dan agronomi seperti aplikasi teknik pemupukan yang efisien dan rasio-nal diperlukan guna menghasilkan produk-si simplisia dengan kandungan bahan aktif tinggi. Penentuan jaringan daun yang te-pat sebagai bahan diagnostik status hara N, P, dan K guna menetapkan kebutuhan pupuk yang efisien bagi tanaman sangat diperlukan. Untuk itu telah dilakukan pene-litian yang menggunakan model korelasi li-nier sederhana yang dilanjutkan dengan uji korelasi. Penelitian ini dilakukan pada tanaman pegagan (Centella asiatica) akse-si Boyolali di KP. Gunung Putri, Cipanas, Balai Penelitian Tanaman Obat dan Aroma-tik (Balittro) sejak Mei sampai Nopember 2008 dengan jenis tanah Andosol yang berada pada ketinggian tempat 1.500 m dpl. Hasil uji korelasi jaringan daun yang paling tepat sebagai bahan diagnosis sta-tus hara bagi produk simplisia dan asiati-kosida pada tanaman pegagan umur 5 bulan setelah tanam (BST) adalah posisi daun ke-3 untuk analisis hara N, P, dan K. Kandungan asiatikosida pada daun tua (1,92% pada umur 6 BST) lebih tinggi dari pada daun muda (1,05% pada umur 3 BST).

Ukuran Diameter dan Takaran Vermikompos Menentukan Produksi Inokulan Fungi Mikoriza Arbuskula dan Biomassa Legum Penutup Tanah Abimanyu D. Nusantara; Cecep Kusmana; Irdika Mansur; Latifah K. Darusman; Soedarmadi Soedarmadi
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 16, No 1 (2011): February 2011
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v16i1.53

Vermikompos merupakan pupuk organik yang diproduksi dengan bantuan sistem pencernaan dan mikrob dalam usus cacing tanah. Vermikompos diketahui berpengaruh positif terhadap pertumbuhan tanaman dan perkembangan simbiosis mikoriza. Penelitian ini bertujuan mencari ukuran diameter dan bobot vermikompos yang optimal untuk menghasilkan biomasa tanaman kudzu (P. phaseoloides Roxb) dan inokulum fungi mikoriza arbuskula (FMA) G. etunicatum NPI-126. Percobaan rumah kaca dilaksanakan dengan rancangan acak kelompok dengan kombinasi ukuran diameter dan bobot vermikompos sebagai perlakuan. Hasil percobaan menunjukkan bahwa vermikompos berpotensi positif sebagai pengganti pupuk buatan untuk meningkatkan produksi biomassa tanaman kudzu dan inokulum FMA G. etunicatum NPI-126. Vermikompos dengan ukuran diameter < 250 Âµm bobot 150â€“172 mg menghasilkan bobot kering akar dan kolonisasi FMA di akar tanaman kudzu serta jumlah spora G. etunicatum tertinggi dibandingkan perlakuan lainnya. Kolonisasi FMA di akar tanaman berkorelasi positif dengan jumlah spora G. etunicatum.

PENETAPAN BAHAN DIAGNOSIS STATUS HARA NPK PADA JARINGAN TANAMAN PEGAGAN Hermanto Hermanto; Munif Ghulamahdi; Latifah K. Darusman; Nurliani Bermawie; Atang Sutandi
Buletin Penelitian Tanaman Rempah dan Obat Vol 22, No 2 (2011): BULETIN PENELITIAN TANAMAN REMPAH DAN OBAT
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/bullittro.v22n2.2011.%p

Studi fisiologi dan agronomi seperti aplikasi teknik pemupukan yang efisien dan rasio-nal diperlukan guna menghasilkan produk-si simplisia dengan kandungan bahan aktif tinggi. Penentuan jaringan daun yang te-pat sebagai bahan diagnostik status hara N, P, dan K guna menetapkan kebutuhan pupuk yang efisien bagi tanaman sangat diperlukan. Untuk itu telah dilakukan pene-litian yang menggunakan model korelasi li-nier sederhana yang dilanjutkan dengan uji korelasi. Penelitian ini dilakukan pada tanaman pegagan (Centella asiatica) akse-si Boyolali di KP. Gunung Putri, Cipanas, Balai Penelitian Tanaman Obat dan Aroma-tik (Balittro) sejak Mei sampai Nopember 2008 dengan jenis tanah Andosol yang berada pada ketinggian tempat 1.500 m dpl. Hasil uji korelasi jaringan daun yang paling tepat sebagai bahan diagnosis sta-tus hara bagi produk simplisia dan asiati-kosida pada tanaman pegagan umur 5 bulan setelah tanam (BST) adalah posisi daun ke-3 untuk analisis hara N, P, dan K. Kandungan asiatikosida pada daun tua (1,92% pada umur 6 BST) lebih tinggi dari pada daun muda (1,05% pada umur 3 BST).

KEANEKARAGAMAN JENIS TUMBUHAN OBAT DARI HUTAN KERANGAS Kissinger Kissinger; Ervizal AM. Zuhud; Latifah K. Darusman; Iskandar Z. Siregar
Jurnal Hutan Tropis Vol 1, No 1 (2013): Jurnal Hutan Tropis Volume 1 Nomer 1 Edisi Maret 2013
Publisher : Lambung Mangkurat University-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/jht.v1i1.1479

Identifikasi tumbuhan obat dari hutan kerangas dalam penelitian ini dilaksanakan dengan metode wawancara semi terstruktur. Penduduk desa yang tinggal di sekitar hutan kerangas diwawancarai menyangkut pengetahuan etnobotani. Penelitian dilakukan di satu lokasi utama hutan kerangas, yaitu Desa Guntung Ujung Kabupaten Banjar Kalimantan Selatan. Tiga lokasi penelitian dipilih sebagai lokasi referensi penelitian: i) Kotawaringin Timur Kalimantan Tengah, ii) Nyaru Menteng Kalimantan Tengah, iii) Tanjung-Kelanis Kalimantan Selatan-Kalimantan Tengah. Hasil penelitian menunjukkan bahwa hutan kerangas kerangas memiliki 36 jenis tumbuhan yang digunakan sebagai bahan pengobatan. Beberapa potensi bioaktivitas yang dapat dikembangkan berdasarkan pengetahuan tradisional di antaranya adalah sebagai antibakteri, antioksidan, antimalaria, antihipertensi, dan andiabetes. Di samping itu, terdapat manfaat lain dari penggunaan atau keberadaan jenis tumbuhan hutan kerangas. Penduduk desa menggunakan berbagai jenis tumbuhan untuk bahan perabotan, pangan, penggunaan spiritual, tanaman hias, bahan pewarna, bahan kerajinan dan kayu bakar. Berdasarkan hasil tersebut dapat disimpulkan bahwa keanekaragaman jenis tumbuhan di hutan kerangas mempunyai fungsi potensial sebagai sumber penghasil tumbuhan bermanfaat.Kata kunci: keanekaragaman jenis, tumbuhan obat, manfaat, hutan kerangas

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