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All Journal Jurnal Primatologi Indonesia Journal of Food and Pharmaceutical Science Jurnal Sain Veteriner Buletin Peternakan Jurnal Veteriner Biogenesis: Jurnal Ilmiah Biologi Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Kedokteran Hewan
Trini Susmiati
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Immunology & microbiology Medicine & Pharmacology Veterinary Other

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Subchronic Toxicity Test of Kebar Grass Extract of n-Hexane (Biophytum Petersianum Klotzsch) on Weight, ADG and Vital Organ Weight of Rat (Rattus Norvegicus) Sambodo, Priyo; Nurhayati, Dwi; Purwaningsih, Purwaningsih; Susmiati, Trini; Airin3, Claude Mona
Journal of Food and Pharmaceutical Sciences Vol 6, No 1 (2018): J. Food Pharm. Sci (January-April)
Publisher : Fakultas Farmasi, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (340.705 KB) | DOI: 10.14499/jfps

Abstract

The recent study was carried out to determine the effect of Kebar grass extract of N-hexane  (Biophytum petersianum Klotzsch) for 28 days peroral on weight, ADG and weight of vital organs of white rat. Forty male and female rats of Sprague Dawley strain aged 8 weeks were examined. The formulated rats were divided into four groups, group I (control), group II, III, IV (treatment), in which each consisted of five rats. The feed were given as much as 15 g/rat/day, while drinking ad libitum. In the control group, the rats were given solvent extract, group II, III and IV were given fraction of n-hexane of Kebar grass extract at 1 mg/kg BW, 5 mg/kg BW and 10 mg/kg BW peroral, respectively. Test preparation was given daily for 28 days, observation of toxic symptoms was performed every day while weighing was performed once every two weeks. Based on results obtained in this line of research, the findings indicated that Kebar extract administration for 28 days did not cause toxic symptoms in both male and female rats either in dose II, III or IV. Weight and ADG of male and female rats did not indicate significant differences between the control group and the treatment group. Keywords:  kebar grass, rats, subcronic toxicity test, OECD 407
PENENTUAN JENIS KELAMIN BURUNG KENARI (Serinus canaria) BERDASARKAN GEN Chromodomain Helicase DNA-Binding 1 (CHD1) Akhrom, Afif Muhammad; Soedarmanto, Indarjulianto; Yanuartono, Yanuartono; Susmiati, Trini; Nururrozi, Alfarisa; Raharjo, Slamet
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 7 No. 1 (2020): June 2020
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (138.004 KB) | DOI: 10.29122/jbbi.v7i1.3178

Abstract

Phenotype determination of sex in young canaries is very low in accuracy. This study aimed to develop a genotypic sexing method in canaries. This study used 12 canaries consisting of 3 mature males, 3 mature females and 6 one-month-old canaries. Phenotypic sexing by cloacal observation was done on all birds, continued by genotypic sexing to identification CHD1 gene using polymerase chain reaction (PCR). The PCR used blood samples for mature canaries, and feather for mature and one-month-old canaries. The results of phenotypic observations showed that all mature male canaries had prominent and pointed cloaca forms, all mature females had flat and wide, whereas all one-month-old birds had a flat cloaca. The result of PCR showed a single band (500 bp) for mature male and double bands (500 bp and 300 bp) for mature female canaries. The PCR results of one-month-old canaries showed that there were one male and five females. Based on this study, it was concluded that genotypic sexing using the PCR method is effective in the sex determination of canaries.Keywords: canary, CHD1, genotype, PCR, sexing ABSTRAKPenentuan jenis kelamin burung kenari muda secara fenotip akurasinya sangat rendah. Penelitian ini bertujuan untuk menentukan jenis kelamin burung kenari secara genotip. Penelitian ini menggunakan 12 ekor burung kenari, terdiri dari 6 ekor dewasa (3 jantan, 3 betina) serta 6 ekor umur 1 bulan. Semua burung ditentukan jenis kelaminnya dengan mengamati kloaka dan identifikasi gen CHD1 menggunakan teknik polymerase chain reaction (PCR). Sampel DNA berasal dari darah dan bulu untuk burung dewasa serta bulu untuk burung umur 1 bulan. Pengamatan fenotip menunjukkan bahwa burung kenari dewasa jantan mempunyai bentuk kloaka menonjol dan runcing, dewasa betina berbentuk datar dan lebar, sedangkan semua burung umur 1 bulan mempunya bentuk kloaka datar. Hasil identifikasi gen CHD1 diperoleh adanya 1 pita gen sekitar 500 bp dari sampel darah dan bulu semua burung kenari dewasa jantan, dan 2 pita gen sekitar 500 bp dan 300 bp dari sampel semua burung kenari betina dewasa. Hasil PCR pada sampel burung umur 1 bulan menunjukkan bahwa 1 ekor jantan dan 5 ekor betina. Berdasarkan penelitian ini dapat disimpulkan bahwa penentuan jenis kelamin secara genotip menggunakan gen CHD1 dapat dilakukan pada burung kenari.
Swab Bukal Sebagai Bahan Sexing Piyikan Burung Kenari (Serinus canaria) dan Burung Merpati (Columba livia) Akrom, Afif Muhammad; Indarjulianto, Soedarmanto; Yanuartono, Yanuartono; Susmiati, Trini; Nururrozi, Alfarisa; Raharjo, Slamet; Permana, Rief Ghulam Satria; Sitompul, Yeremia Yobelanno
Jurnal Sain Veteriner Vol 38, No 1 (2020): April
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1012.071 KB) | DOI: 10.22146/jsv.57364

Abstract

Molecular sexing for bird with polymerase chain reaction-based method have been developed, but the samples used as a sources of DNA are blood and feathers which are considerably invasive. The aim of this study was to study the efficiency of buccal swabs as a resource of DNA for sexing canary bird and pigeon. This study used 10 canaries (Serinus canaria) consisting of 6 adult canaries (3 males and 3 females) and 4 young nestling canaries (14 - to 18-day old) and 6 adults (3 males and 3 females) pigeons (Columba livia) and 7 young nestling pigeons (14- to 25-day-old). All birds were taken their buccal swab samples, then DNA were extracted, mixed with PCR-mix to be amplified for sexing genes with CHD1F/CHD1R primer pairs. The amplification results showed that all of adult male birds produced single band (± 500 bp), whereas all of adult female birds produced double bands (± 500 bp and ± 300 bp). The PCR method for nestling canaries showed 2 males and 2 females. whereas nestling pigeons 6 males and 1 female. Based on this study it can be concluded that buccal swabs are efficient as a source of DNA for birds sexing especially young nestling birds. 
PENENTUAN JENIS KELAMIN BURUNG KENARI (Serinus canaria) BERDASARKAN GEN Chromodomain Helicase DNA-Binding 1 (CHD1) Afif Muhammad Akhrom; Indarjulianto Soedarmanto; Yanuartono Yanuartono; Trini Susmiati; Alfarisa Nururrozi; Slamet Raharjo
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 7 No. 1 (2020): June 2020
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (138.004 KB) | DOI: 10.29122/jbbi.v7i1.3178

Abstract

Phenotype determination of sex in young canaries is very low in accuracy. This study aimed to develop a genotypic sexing method in canaries. This study used 12 canaries consisting of 3 mature males, 3 mature females and 6 one-month-old canaries. Phenotypic sexing by cloacal observation was done on all birds, continued by genotypic sexing to identification CHD1 gene using polymerase chain reaction (PCR). The PCR used blood samples for mature canaries, and feather for mature and one-month-old canaries. The results of phenotypic observations showed that all mature male canaries had prominent and pointed cloaca forms, all mature females had flat and wide, whereas all one-month-old birds had a flat cloaca. The result of PCR showed a single band (500 bp) for mature male and double bands (500 bp and 300 bp) for mature female canaries. The PCR results of one-month-old canaries showed that there were one male and five females. Based on this study, it was concluded that genotypic sexing using the PCR method is effective in the sex determination of canaries.Keywords: canary, CHD1, genotype, PCR, sexing ABSTRAKPenentuan jenis kelamin burung kenari muda secara fenotip akurasinya sangat rendah. Penelitian ini bertujuan untuk menentukan jenis kelamin burung kenari secara genotip. Penelitian ini menggunakan 12 ekor burung kenari, terdiri dari 6 ekor dewasa (3 jantan, 3 betina) serta 6 ekor umur 1 bulan. Semua burung ditentukan jenis kelaminnya dengan mengamati kloaka dan identifikasi gen CHD1 menggunakan teknik polymerase chain reaction (PCR). Sampel DNA berasal dari darah dan bulu untuk burung dewasa serta bulu untuk burung umur 1 bulan. Pengamatan fenotip menunjukkan bahwa burung kenari dewasa jantan mempunyai bentuk kloaka menonjol dan runcing, dewasa betina berbentuk datar dan lebar, sedangkan semua burung umur 1 bulan mempunya bentuk kloaka datar. Hasil identifikasi gen CHD1 diperoleh adanya 1 pita gen sekitar 500 bp dari sampel darah dan bulu semua burung kenari dewasa jantan, dan 2 pita gen sekitar 500 bp dan 300 bp dari sampel semua burung kenari betina dewasa. Hasil PCR pada sampel burung umur 1 bulan menunjukkan bahwa 1 ekor jantan dan 5 ekor betina. Berdasarkan penelitian ini dapat disimpulkan bahwa penentuan jenis kelamin secara genotip menggunakan gen CHD1 dapat dilakukan pada burung kenari.
Respon Ekspresi Molekul Adhesi pada Kultur Sel Endotel yang diinduksi LDL Teroksidasi dari Macaca fascicularis terhadap Ekstrak Kurkuminoid Temumangga Trini Susmiati; Sulistiyani .; Dondin Sajuthi; Latifah K. Darusman
Jurnal Primatologi Indonesia Vol. 6 No. 2 (2009)
Publisher : Pusat Studi Satwa Primata LPPM-IPB

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (413.298 KB)

Abstract

Curcuminoids of Curcuma manggo believed to have antioxidant properties that can prevent the occurrence of atherosclerosis.  However, the mechanism at the molecular level is not clearly known. Increase in intracellular adhesion molecule-1 (ICAM-1) will result in endothelial cell dysfunction that initiates athersclerosis. The expression of ICAM-1 can be triggered by oxidized LDL molecules. The purpose of this study is to elucidate the effects of curcuminoids temumangga (Curcuma mango) on the expression of ICAM-1 induction in endothelial cell culture. Prior to the induction of the expression of ICAM-1, endothelial cell culture was pre-incubated with curcuminoids extract for 48 hours with concentration of 2 ppm and 8 ppm, then further incubated with oxidized LDL for 24 hours. The treated cells were compared to 5 µM Cu2+ induced endothelial cell culture with or without LDL  from Macaca fascicularis.  ICAM-1 expression was determined by immunohistochemistry using anti-ICAM-1 antibodies and visualized with staining by 3.3-diaminobenzidin tetrahidrochloride (DAB). Results showed that surface-induced endothelial cell culture with ion Cu2+ stained well by DAB as brown yellow, which indicates ICAM-1 expression. Curcuminoids extract of  8 ppm reduced induction of ICAM-1 response. Induction of ICAM-1 expression by oxidized LDL caused foam cell accumulation in addition to ICAM-1 expression.Key words: Curcuminoid, low density lipoprotein (LDL), Macaca fascicularis, intracellular adhesion molecule-1 (ICAM-1), immunohistochemistry
Beak Line and Eye Alignment as Phenotypic Sexing for Domestic Canaries (Serinus canaria) Afif Muhammad Akrom; Soedarmanto Indarjulianto; Yanuartono Yanuartono; Trini Susmiati; Alfarisa Nururrozi; Slamet Raharjo; Rief Ghulam Satria Permana; Puveanthan Nagappan Govendan
Biogenesis: Jurnal Ilmiah Biologi Vol 8 No 1 (2020)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v8i1.11115

Abstract

Phenotypic sexing of birds is a common practice among the songbird-keeping community, yet it is based on non-reputable information. This study aims to determine the sex of canaries (Serinus canaria) based on the alignment of the eye with the beak line. A total of 26 samples, consisting of six samples of one-month-old canaries (three males and three females based on PCR examination), 20 samples of six-month-old canaries (ten proven breeding pairs) were used in this study. The birds' heads were photographed from the sides, and then the positions of the eyes were compared with the shadow alignment of the beak. The results provided that five young birds and ten adult birds have a beak line alignment under the eye, while ten adult birds and one young bird have a beak line alignment across the eye. The accuracy of sexing using this method was 100% for both female and male adult canaries. However, this result could not be applied to sample that are not yet sexually mature, as two young male birds were found to have beak alignment below the eye. It can be concluded that sexing adult canaries can be performed by observing the eye and beak line's alignment.
Uji Ekstrak n-Hexana Rumput Kebar (Biophytum petersianum Klotzsch) pada Tikus Wistar Hiperkolesterolemia Priyo Sambodo; Purwaningsih Purwaningsih; Alnita Baaka; Trini Susmiati; Claude Mona Airin
Jurnal Sain Veteriner Vol 37, No 1 (2019): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (493.167 KB) | DOI: 10.22146/jsv.48487

Abstract

This study was aimed to determine the secure level of the n-hexane extract of kebar grass on hypercholesterolemia rats based the acute oral toxicity test. Based the literature, no report on the toxic dosage before, initial dose was started at 300 mg/kg body weight (BW) and to be increased 2000 to 5000 mg/kg BW. Toxicity data were set for 24 hours and continued until 14 days after treatment for several parameters, i.e : toxicity symptoms, death of animals, changes in body weight and the manifestation of toxicity effects. At the end of the test, the rats were sacrificed and organs were taken for abnormalities (macroscopy). The results of this study showed that the administration of the n-hexane extract of grass kebar at all doses did not show the toxicity symptoms, mortality and body weight change. The absolute and relative weights and gross pathology observation of the internal organ were normal. Conclusions: the n-hexane extract of grass kebar is safe and the LD was determined on category 5, 50 unclassified or minimum practically non toxic
Swab Bukal Sebagai Bahan Sexing Piyikan Burung Kenari (Serinus canaria) dan Burung Merpati (Columba livia) Afif Muhammad Akrom; Soedarmanto Indarjulianto; Yanuartono Yanuartono; Trini Susmiati; Alfarisa Nururrozi; Slamet Raharjo; Rief Ghulam Satria Permana; Yeremia Yobelanno Sitompul
Jurnal Sain Veteriner Vol 38, No 1 (2020): April
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.49032

Abstract

Teknik sexing pada burung secara molekuler dengan metode PCR telah banyak dikembangkan, tetapi sampel yang digunakan adalah darah dan bulu yang dianggap invasif. Penelitian ini bertujuan untuk mempelajari efisiensi sampel swab bukal sebagai sumber DNA dalam sexing dengan metode PCR. Penelitian ini menggunakan 10 ekor burung kenari (Serinus canaria) yang terdiri dari 6 ekor burung dewasa (3 jantan dan 3 betina) dan 4 ekor kenari piyikan (umur 14 – 18 hari) yang belum diketahui jenis kelaminnya serta 6 ekor merpati (Columba livia) dewasa (3 jantan dan 3 betina) dan 7 ekor merpati piyikan (umur 14 – 25 hari) yang belum diketahui jenis kelaminnya. Amplifikasi fragmen gen dilakukan menggunakan metode PCR dengan pasangan primer CHD1F/CHD1R.Hasil visualisasi produk PCR menunjukkan semua burung jantan dewasa menghasilkan satu band (± 500 bp), sedangkan burung betina dewasa menghasilkan dua band (± 500 bp dan ± 300 bp). Amplifikasi gen dari swab bukal burung kenari muda didapatkan 2 ekor jantan dan 2 ekor betina, sedangkan dari swab bukal burung merpati muda didapatkan 6 ekor jantan dan 1 ekor betina. Berdasarkan hasil penelitian ini dapat disimpulkan bahwa sampel swab bukal terbukti efisien sebagai sumber DNA dalam sexing burung khususnya burung piyikan.
Refeeding Postmolting Method to Improve Weekly Production Performance of Rejected Laying Hens with Low Mortality Claude Mona Airin; Amelia Hana; Pudji Astuti; Sarmin Sarmin; Trini Susmiati; Okti Herawati
Buletin Peternakan Vol 44, No 2 (2020): BULETIN PETERNAKAN VOL. 44 (2) MAY 2020
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v44i2.45508

Abstract

This research aimed to investigate the influence of gradually feeding rejected laying hens after molting on the performance of production. This research used 6,000 rejected laying hens of 80 weeks old in Subur Farm. Molting method was a modification method by reducing the feed gradually. At the beginning of this research, the feed was given 120 g/laying hens/day and it would be reduced by 10 g/laying hens/day until it reach 50 g/laying hens/day. In the next phase, all chickens fasted for seven days then the chicken was given 10 g/laying hens on the first day. The feed was increased 10 g/laying hens every two days up to 120 g/laying hens/day. Data Collection of Feed conversion ratio (FCR), mortality rate, amount of feed, and egg production were taken at the start of re-feeding. The results showed that during the period of molting until refeeding, mortality was 3.6%, an increase in egg production was seen since week 1, peak of production was 78% at week 9 with FCR 2.3.  The results of statistical analysis showed significant differences (P<0.05) on the percentage of egg production between time periods after the treatment of feed reduction. As the conclusion, molting followed by refeeding in rejected laying hen influences weekly production performance by extending peak production period, optimal FCR and daily egg production.
Kandungan L-3, 4-dihydroxyphenylalanine Suatu Bahan Neuroprotektif pada Biji Koro Benguk (Mucuna pruriens) Segar, Rebus, dan Tempe (L-3,4-DIHYDROXYPHENYLALANINE CONTENT AS A NEUROPROTECTIVE MATERIAL ON FRESH, COOKED AND FERMENTED OF KORO BENGUK (MUCUNA PR Tri Wahyu Pangestiningsih; Trini Susmiati; Hery Wijayanto
Jurnal Veteriner Vol 18 No 1 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (129.322 KB) | DOI: 10.19087/jveteriner.2017.18.1.116

Abstract

Indonesia is rich in flora potentially used for herbal medication. One of the potential herbal is koro benguk (Mucuna pruriens) beans, where in Central Java and Yogyakarta is proccessed into tempe (fermented mucuna beans) for daily human consumption. Koro benguk has high level of L-3,4-dihydroxyphenylalanine (L-DOPA) which has a potential neuroprotective effect on Parkinson’s disease. The aim of this study was to investigate the L-DOPA content in fresh beans, cooked and fermented of koro benguk beans. The investigation were done in fresh mucuna beans, white color (BR D) and black color (BR A) beans originated from Wonogiri, Central Java, and fresh, white color (KP C), cooked, and fermented beans collected from Kulon Progo, Yogyakarta. The samples were extracted using ethanol and n-propanol solutions and were analyzed using high-performance liquid chromatography (HPLC) technique. The results show that the highest L-DOPA level (8,56%) was found in fresh white koro benguk beans from Wonogiri extracted using ethanol. The lowest L-DOPA level (0,016%) was found in fermented beans that extracted using n-propanol. Extraction using ethanol yield a higher L-DOPA level as compared to that of using n-propanol. In brief, all of the samples starting from fresh bean, cooked, and fermented koro benguk beans contain L-DOPA, with highest L-DOPA level was found in the white fresh koro benguk beans, from Wonogiri, Central Java. The lowest ingredient L-DOPA level was found in the fermented beans from Kulon Progo, Yogyakarta. ABSTRAK Indonesia sangat kaya dengan keanekaragaman flora yang potensial untuk terapi herbal, salah satunya tanaman koro benguk (Mucuna pruriens) yang bijinya bisa diolah menjadi tempe sebagai konsumsi harian masyarakat di sekitar Yogyakarta dan Jawa Tengah. Biji koro benguk diketahui mengandung L-3,4-dihydroxyphenylalanine (L-DOPA) tinggi dan berpotensi menjadi agen neuroprotektor pada penyakit Parkinson. Penelitian ini bertujuan untuk menguji kandungan L-DOPA mulai dari biji koro benguk segar, rebus, dan bahan olahannya yaitu tempe benguk. Uji dilakukan pada biji koro benguk mentah kulit berwarna putih (BR D) dan hitam (BR A) asal Wonogiri, Jawa Tengah, serta biji koro benguk mentah kulit berwarna putih (KP C), biji koro benguk yang sudah direbus dua kali, dan tempe benguk asal Kulon Progo, Yogyakarta. Sampel diekstraksi menggunakan pelarut etanol dan n-propanol, kemudian dianalisis dengan teknik high-performance liquid chromatography (HPLC) untuk melihat kadar kandungan L-DOPA-nya. Hasil penelitian menunjukkan, kadar L-DOPA tertinggi (8,56%) ditemukan pada biji koro benguk mentah dengan warna kulit putih asal Wonogiri yang diekstraksi menggunakan pelarut etanol, sedangkan kadar L-DOPA terendah (0,016%) ditemukan pada sediaan tempe yang diekstraksi dengan n-propanol asal Kulon Progo. Secara umum, ekstraksi menggunakan pelarut etanol memberikan hasil kadar L-DOPA yang lebih tinggi dibandingkan dengan pelarut n-propanol. Semua ekstraksi sampel, mulai biji koro benguk segar, rebus sampai bentuk tempenya mengandung L-DOPA, dengan kadar tertinggi terdapat pada biji koro benguk segar berkulit putih asal Wonogiri, Jawa Tengah yang diekstraksi menggunakan etanol, sedangkan kadar terendah dijumpai pada tempe benguk dari Kulon Progo, Yogyakarta yang diekstraksi menggunakan n-propanol.
Co-Authors A.A. Ketut Agung Cahyawan W Afif Muhammad Akhrom Afif Muhammad Akrom Afif Muhammad Akrom Akhrom, Afif Muhammad Akrom, Afif Muhammad Alfarisa Nururrozi Alfarisa Nururrozi Alfarisa Nururrozi Alfarisa Nururrozi, Alfarisa Alnita Baaka Amelia Hana Aris Purwantoro Aris Purwantoro Claude Mona Airin Dondin Sajuthi Dwi Nurhayati, Dwi Herawati, Okti Hery Wijayanto Laksono Trisnantoro Latifah K. Darusman Permana, Rief Ghulam Satria Priyo Sambodo Pudji Astuti Purwaningsih - Purwaningsih Purwaningsih Puveanthan Nagappan Govendan Rief Ghulam Satria Permana Rief Ghulam Satria Permana Rini Widayanti Rini Widayanti Rini Widayanti Rini Widayanti Sarmin Sarmin Sitompul, Yeremia Yobelanno Slamet Raharjo Slamet Raharjo Slamet Raharjo Soedarmanto Indarjulianto Sulistiyani . Tri Wahyu Pangestiningsih Wayan Tunas Artama Yanuartono Yanuartono Yanuartono Yanuartono Yanuartono Yanuartono Yanuartono, Yanuartono