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All Journal PROSIDING SEMINAR NASIONAL El-Hayah : Jurnal Biologi Jurnal Kesehatan INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY
Sri Sinto Dewi
Program Pascasarjana, Program Studi Magister Ilmu Biomedik Universitas Diponegoro, Jl. Hayam Wuruk No. 5 Lt. III Semarang - 50241
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Social Sciences

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EFEKTIFITAS VIRGIN COCONUT OIL ( VCO ) TERHADAP KANDIDIASIS SECARA INVITRO Dewi, Sri Sinto; Aryadi, Tulus
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2010: PROSIDING SEMINAR NASIONAL HASIL-HASIL PENELITIAN
Publisher : Universitas Muhammadiyah Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (45.248 KB)

Abstract

Minyak kelapa murni Virgin Coconut Oil (VCO ) merupakan minyak kelapa yang diproses tanpapemanasan yaitu dengan penambahan enzim, pancingan, sentrifugasi. Minyak kelapa dari proses tersebutbanyak mengandung asam lemak jenuh rantai sedang, yang diyakini sebagai herbal alternative untukpengobatan dan pencegahan penyakit degeneratif maupun penyakit yang disebabkan mikroorganisme.Kandungan vco yang paling banyak adalah asam lemak jenuh rantai sedang diantaranya : asam laurat,asam kaprilat, asam miristrat, asam palmirat dll yang mudah dicerna oleh tubuh menjadi energi yang siapdipakai. VCO juga diyakini sebagai obat keputihan yang disebabkan oleh jamur Candida albicans.Penelitian ini dilakukan di Laboratorium Mikrobiologi Fakultas Ilmu Keperawatan dan KesehatanUNIMUS pada bulan Mei sampai dengan Agustus 2007. Sampel berupa Candida albicans isolateSemarang dan VCO dari beberapa merk Penelitian dilakukan dengan uji sensitifitas vco terhadapCandida albicans dengan metoda sumuran dan kandistatin ( antifungi ) sebagi control .Dari penelitian ini memberikan hasil bahwa vco tidak dapat menghambat pertumbuhan Candidaalbicans secara invitro .Kata kunci : Efektifitas, VCO, Kandidiasis
AKTIVITAS LACTOBACILLUS PLANTARUM ISOLAT AIR SUSU IBU PADA TIKUS GALUR WISTAR DIABETES MELLITUS Kartikasari, Oktavia; Astuti, Anggun Dian; Wabula, Mega Berkah Mustika; Dewi, Sri Sinto
Jurnal Kesehatan Vol 10, No 3 (2019): Jurnal Kesehatan
Publisher : Politeknik Kesehatan Tanjung Karang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (710.295 KB) | DOI: 10.26630/jk.v10i3.1488

Abstract

Lactobacillus plantarum is a Lactic Acid Bacteria (LAB) that has the potential to be used as a probiotic agent. Probiotics are antidiabetic because they can inhibit alpha-glucosidase enzymes. This study aims to was to determine the antidiabetic effects of probiotic Lactobacillus plantarum isolates of breast milk (ASI). The study was conducted at the Microbiology Laboratory of the University of Muhammadiyah Semarang and the Pharmacology Laboratory of the Semarang College of Pharmacy during the month of April-June. Experimental research with 25 male wistar mice was divided into 5 groups (K-, K+, P1, P2, P3) Alloxan monohydrate induction was carried out intraperitoneally at a dose of 150mg/kg BW. Fasting blood glucose measurements were performed at baseline (before alloxan induction), post alloxan and at the end of treatment. Diabetic mice fasting blood glucose levels shouldbe± 200 mg/dL. Suspension of Lactobacillus plantarum isolate ASI was carried out orally for 7 days. Based on the ANOVA test there were significant differences in the decrease in blood glucose with a significant value of p-value=0,009<0,05. Based on the Tuckey post hoc test treatment group P1, P2, P3 there were no significant differences between treatment groups even though the dosage was different. 1 time a day treatment is an efficient dose in reducing blood glucose.
Bacterial Plasmids Profile from Escherichia coli Resistant to Metronidazole and Nalidixic Acid Iswara, Arya; Dewi, Sri Sinto
El-Hayah : Jurnal Biologi Vol 6, No 1 (2016): EL-HAYAH (VOL 6, NO 1,September 2016)
Publisher : Department of Biology Science and Technology Faculty UIN Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/elha.v6i1.4079

Abstract

bacteria that cause an illness. Antibiotic treatments to a patient have a purpose to eliminate the pathogen bacteria. Bacteria resistance to antibiotic was influenced by the intensity of antibiotic treatment in a region, the uncontrolled antibiotics treatments would increase the antibiotic resistance of bacteria. Plasmids was an extrachromosomal DNA that encodes a functional protein that would eliminate the antibiotic activity. Plasmid is the determinant of bacteria sensitivity to antibiotics. In this case it would be important to find out the bacterial plasmid profile on the E.coli resistant to metronidazole and nalidixic acid antibiotics. This research was using four different sample from faces of diarrhea, ice block, waters from well, and ketchup to cultivate the E. coli. lasmid isolation method was carried out by lyses alkali method. Plasmid profile of the E. coli that resistant to metronidazole and nalidixic acid antibiotics and analyzed using electrophoresis on 1% agarose. E. coli plasmid DNA profile was observed as a fluorescent DNA band in ultraviolet rays. In result, isolated plasmids from bacteria that resistant to antibiotics metronidazole and nalidixic acid having similar size approximately 500 bp, different from bacteria that sensitive to antibiotics metronidazole and nalidixic acid has a smaller size in region of 100 bp.
Exploration and molecular identification of proteolytic bacteria from rusip pacific oyster (Crassostrea gigas) as anticoagulant agent candidates Afriansyah, Muhammad Ardi; Sudarwin; Dewi, Sri Sinto; Akbar, Gusti Dimas Refian
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 7 No 1 (2025): Advances in biomarkers, therapeutics, and probiotics: Recent updates in medical l
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v7i1.6083

Abstract

The marine symbiont Staphylococcus epidermidis strain CGF-6, a protease-producing bacterium, has been successfully isolated from Rusip Pacific Oyster (Crassostrea gigas). S. epidermidis is a non-spore-forming, Gram-positive coccus commonly found in marine environments due to their ability to tolerate high salinity. The aim of this study was to identify proteolytic bacteria from Rusip fermented C. gigas as potential candidates for the development of anticoagulant agents. Bacterial isolation was performed through the fermentation process of Rusip. After seven days, bacterial colonies were purified three times using Nutrient Agar. The selection of proteolytic bacterial was conducted qualitatively using a skim milk agar medium. The bacterial isolates exhibiting the highest protease activity were identified through 16S rRNA gene sequencing using universal primers Bact 27F and UniB 1492R. Phylogenetic tree analysis, conducted with the MEGA X program, helped determine the relationships between species. Out of the 18 bacterial isolates obtained from the Rusip fermentation of C. gigas, three isolates (CGF-1, CGF-6, and CGF-11) exhibited hydrolysis zones around their colonies on skim milk agar, indicating protease activity.  Among these, isolate CGF-6 showed the highest proteolytic index of 0.5 and was identified as Staphylococcus epidermidis strain CGF-6. S. epidermidis strain CGF-6 has the potential to serve as a valuable source of protease production for the development of anticoagulant agents. However, further studies, including enzyme characterisation, optimisation, and both in vitro and in vivo anticoagulant activity tests, are necessary to assess the efficacy and safety of this enzyme as a candidate for anticoagulant agents.
Co-Authors A.A. Ketut Agung Cahyawan W Afriansyah, Muhammad Ardi Akbar, Gusti Dimas Refian Arya Iswara Astuti, Anggun Dian Ernanto, Aditya Rahman Fuad, Hayatun Kartikasari, Oktavia Nur Hidayah Silvestre, Richard David Sri Darmawati Stalis Norma Ethica Sudarwin Sulistyaningtyas, Ayu Rahmawati Tulus Aryadi Wabula, Mega Berkah Mustika