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All Journal Indonesian Journal of Biotechnology BERITA BIOLOGI JURNAL BIOLOGI INDONESIA JURNAL PANGAN Indonesian Journal of Pharmacology and Therapy
Achmad Dinoto
Research Center for Biology, Indonesian Institute of Sciences (LIPI). Jalan Raya Jakarta-Bogor KM 46, Cibinong 16911, Jawa Barat, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Social Sciences

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Journal : BERITA BIOLOGI

 1 
VARIASI GENETIK Lactobacillus fermentum Beijerink ASAL SAYUR ASIN BERDASARKAN ANALISIS RFLP 16S-23S rDNA ISR, RAPD-PCR DAN ERIC-PCR [Genetic Variation of Lactobacillus fermentum Beijerink Origin Sayur Asin Based on RFLP 16S-23S rDNA ISR, RAPD-PCR and ERIC-PCR Analysis] Sulistiani, Sulistiani; Mangunwardoyo, Wibowo; Abinawanto, Abinawanto; Sukara, Endang; Dinoto, Achmad; Salamah, Andi
BERITA BIOLOGI Vol 16, No 2 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3242.558 KB) | DOI: 10.14203/beritabiologi.v16i2.2772

Abstract

Molecular analysis of Lactobacillus fermentum isolates is essential to understand their genetic variation in relations to their roles in sayur asin fermentation process. Combination of three molecular techniques which is restriction fragment length polymorphism (RFLP) of 16S23S rDNA intergenic spacer region (ISR), random amplified polymorphic DNA (RAPD-PCR) and an enterobacterial repetitive intergenic consensus (ERIC-PCR) analysis were performed to discriminate 19 representative isolates of L. fermentum isolated from sayur asin. The result showed that L. fermentum strain D11 is distantly related to other isolates based on RFLP using HhaI restriction enzyme and RAPDPCR analyses. In addition, both of RAPD-PCR and ERIC-PCR successfully determined the genetic variation among L. fermentum strains by exhibiting distinct 4-8 bands (800-2080 bp) and 4-10 bands (280-3050 bp), respectively. A dendogram generated from UPGMA cluster analysis of both RAPD-PCR and ERIC-PCR data showed two distinct genotypic groups exist among L. fermentum isolated from sayur asin in Indonesia.
PERUBAHAN KADAR KOLESTEROL SERUM PADA TIKUS SETELAH MENGKONSUMSI MALTOOLIGOSAKARIDA YANG DISINTESIS SECARA ENZIMATIK MENGGUNAKAN AMILASE Bacillus licheniformis BLl [The Change of Serum Cholesterol Level in Rats after Consuming Maltooligosaccharide Synthesized by Enzimatic Reaction of Bacillus licheniformis BLl Amylase] Dinoto, Achmad; Rahayu, Rita Dwi; Satyaningtijas, Aryani S
BERITA BIOLOGI Vol 10, No 1 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1007.167 KB) | DOI: 10.14203/beritabiologi.v10i1.2046

Abstract

To investigate the impact of consuming enzymatically synthesized maltooligosaccharide using Bacillus licheniformis BLl amylase on reduction of serum cholesterol level, in preclinical study groups of Sprague Dawley rats were fed for 3 weeks with four different test diets: basal diet (BD), high serum cholesterol-stimulating basal diet (CD), high serum cholesterol-stimulating basal diet supplemented with 3% (w/w feed) commercial maltooligosaccharide (CI), and high serum cholesterol-stimulating basal diet supplemented with 3% (w/w feed) maltooligosaccharide synthesized by enzimatic reaction of B. licheniformis BLl amylase (CB).Our result shows a reduction of serum cholesterol level up to 39.1% in rats CB after three weeks consuming maltooligosaccharide synthesized using B. licheniformis BLl amylase. In addition, significantly increased blood glucose level was not found in this study as an impact of consuming maltooligosaccharide synthesized using B. licheniformis BLl amylase.
Co-Authors A.S. Satyaningtijas Abinawanto Abinawanto Agustine Susilowati Andi Salamah Aspiyanto Aspiyanto Ema Damayanti Endang Sukara Febriansah , Rifki Hadi, Ismanurrahman JAKA WIDADA Khoirun Nisa Lies Mira Yusiati Lotulunga, Puspa D. Mustofa Mustofa Rita Dwi Rahayu Sulistiani ., Sulistiani Sulistiani Sulistiani, Sulistiani WIBOWO MANGUNWARDOYO