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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Ternak Veteriner agriTECH Indonesian Journal of Biotechnology Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Teknologi Indonesia Al-ihsan : Journal of Community Development in Islamic Studies Indonesian Journal of Pharmacology and Therapy

Ema Damayanti

Balai Pengembangan Proses dan Teknologi Kimia (BPPTK)-LIPI, Yogyakarta

Author-ID : 249110

Religion Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Social Sciences Veterinary Other

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Articles

The use of earthworm meal (Lumbricus rubellus) as anti-pullorum agent in feed additive of broiler chicken Damayanti, Ema; Sofyan, Ahmad; Julendra, Hardi; Untari, Tri
Indonesian Journal of Animal and Veterinary Sciences Vol 14, No 2 (2009)
Publisher : Indonesian Animal Sciences Society

The aim of this research was to study the use of earthworm meal (TCT) L. rubellus as anti pullorum agent in poultry feed additive (IP). The antibacterial activity of TCT against Salmonella pullorum was examined using diffusion agar method at each of the following concentrations: 0, 25, 50, 75 and 100% (w/v) in 100 ÂµL DMSO. In vivo test was conducted using 80 broiler chicken and were infected by S. pullorum with treatments of: IP0: IP contained 0% TCT, IP1: IP contained 25% TCT, IP2: IP contained 50% TCT, IP3: IP contained 75% TCT and IP4: IP contained 100% TCT. Each treatment was replicated 4 times with 4 chicks each. Feed additive was periodically fed to broiler during 7 days before and 10 days after infection. Anti-pullorum activities were evaluated using serology test, isolation and biochemical identification of S. pullorum. The results showed that 75% TCT was optimum to inhibit S. pullorum in vitro. The isolation and identification of S. pullorum results showed that 0 out of 8 (0%) broilers treated with IP4 was not infected by S. pullorum whereas 1 out of 2 (50%) broilers treated with IP0 were infected by S. pullorum. The reduction of S. pullorum prevalence as followed by increasing TCT in feed additive. In conclusion, TCT as poultry feed additive could inhibit S. pullorum infection. Key words: Earthworm Meal, Feed Additive, S. Pullorum

Antibacterial activity and retained protein of earthworm meal (Lumbricus rubellus) as feed additive combined with chitosan Sofyan, Ahmad; Damayanti, Ema; Julendra, Hardi
Indonesian Journal of Animal and Veterinary Sciences Vol 13, No 3 (2008)
Publisher : Indonesian Animal Sciences Society

This research was conducted to enhance the bacterial growth inhibition of E. coli by using earthworm (Lumbricus rubellus) meal (TCT) which was added with chitosan and its effect on the retained protein in broilers. Inhibition of E. coli growth was tested using dilution method on the nutrient broth by additional 2% TCT combined with 0, 0.5, 1.0 and 1.5% chitosan. Retained protein was measured using broiler fed diet containing 2% TCT (w/w) and added by chitosan 0, 0.5, 1.0 and 1.50% of TCT (w/w). The numbers of 15 broilers Cobb strain 35 days old were arranged on Completely Randomized Design (CRD). Results showed inhibition of E. coli was increased using TCT mixed chitosan. The highest inhibition to E. coli growth obtained from TCT + 0.5% chitosan. Retained protein tended to increase up to 1% (w/w) chitosan. Otherwise, chitosan level more than 1% could reduce protein retention. It is concluded that use of 1% chitosan increased TCT capability to inhibit E. coli and protein retention in the broilers. Key Words: Feed Additive, Chitosan, L. rubellus, E. coli

16s rRNA Identification of Pediococcus spp. from Broiler and Studies of Adherence Ability on Immobilized Mucus Damayanti, Ema; Yusiati, Lies Mira; Dinoto, Achmad
Indonesian Journal of Biotechnology Vol 17, No 2 (2012)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (309.077 KB)

The objectives of this research were to study taxonomical status of lactic acid bacteria (LAB) isolated from broiler and adherence ability on mucus in vitro. Molecular analysis was performed by analyzing 16S rRNA gene using universal primer. The adherence assay on mucus was carried out using microplate method with total plate count (TPC), absorbance (A550) and confirmed by scanning electron microscopy (SEM). The results of this studies revealed that three of LAB isolates have closed relation to Pediococcus acidilactici (99.9%) species.Three isolates of P. acidilactici have adherence ability on broiler mucus higher than that on porcine mucin with an adherence percentage of 55.5% versus 50.8% and absorbance A550 of 0.061 versus 0.051, respectively. The highest adherence ability showed by P. acidilactici R02 with adherence percentage was 59.3% and absorbance A550 = 0.068. Adherence on mucus were affected by the addition of 3 g/l of gastric juice and 0.3% (b/v) of bile salt. Adherence analysis using SEM also showed that the adherence on broiler mucus was higher than the adherence on porcine mucin. Altogether this adherence studies, suggest that three isolates of P. acidilactici LAB were capable of colonizing host intestinal mucus in vitro as important property to be promising probiotic bacteria for broiler.Key words : adherence, broiler, Pediococcus, mucus, 16S rRNA

PROFIL ASAM AMINO EKSTRAK CACING TANAH (LUMBRICUS RUBELLUS) TERENKAPSULASI DENGAN METODE SPRAY DRYING Hayati, Septi Nur; Herdian, Hendra; Damayanti, Ema; Istiqomah, Lusty; Julendra, Hardi
Teknologi Indonesia Vol 34 (2011)
Publisher : LIPI Press

Antibiotics in poultry were widely used as growth promotor and anti-infective. When antibiotics were used continuously for long periods, it caused microbial resistance. Various attempts were made to replace antibiotics along with the increasing of trend towards demand for healthy, safe and free from harmful residues of livestock products. One of them was application of earthworm which contains high protein and complete amino acids. Earthworms were extracted into water extract form by decoct method. Water extract form had some weaknesses. It was necessary to formulate into a solid dosage form which was more stable, reproducible, and practical. The selected formulation was encapsulation by spray drying method. Quality control of feed additives can be seen from biological value. Biological value correlated positively with amino acids equilibrium or Essential Amino Acid Index (EAAI). The result showed that earthworm extract had both essential and non-essential amino acids higher than earthworm meal and encapsulated earthworm extract. The highest essential amino acid in earthworm meal was isoleucine (3.14%), in earthworm extract was lysine (8.16%), and in encapsulated earthworm extract was leucine (1.71%). The highest non-essential amino acids in earthworm meal and encapsulation of earthworm extract were glutamic acid, respectively 7.67% and 1.87%, while the earthworm extracts was serine (14.52%). The high value of IAAE in earthworm extract showed that the extraction method resulted better amino acid balance (69.87%) than earthworm meal (58.67%). While the value IAAE of encapsulated earthworm extract showed that levels of essential amino acid balance was lower (16.05%) than the earthworm meal (69.87%) and the earthworm extract (58.67%).

EDUKASI DAMPAK PERNIKAHAN DINI DI DESA RIDOMANAH Damayanti, Ema; Ash Shabah, Musyaffa Amin; Suprihatin, Suprihatin
Al-Ihsan: Journal of Community Development in Islamic Studies Vol 3 No 1 (2024): Al-ihsan : Journal of Community Development in Islamic Studies
Publisher : LPPM Universitas Islam 45

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33558/alihsan.v3i1.9547

Early marriage is a marriage that is carried out by early childhood or has not reached the age allowed for marriage. In law, marriage is allowed when you reach the age of 19 years. However, there are still many people who practice early marriage, especially rural communities. Early marriage has many impacts, one of which is the failure of child growth and development or stunting. In this community service, the goal is to prevent early childhood marriage and stunting in Ridomanah village. In this activity, the subject is students of SMPN 2 Cibarusah, the implementation method is community education with socialization of the impact of early marriage. Based on the percentage of pre-test and post-test results, student knowledge has increased significantly, from 64.20% to 85.40%. This socialization activity on the impact of early marriage at SMPN 2 Cibarusah is quite effective and successful in increasing students' knowledge about the impact of early marriage.

Marine Actinobacteria amo.128 Isolated from Seribu Island: Antibacterial, Antibiofilm and Molecular Docking as Quorum Sensing Inhibitors Cahyanto, Heru Agus; Sunaryanto, Rofiq; Damayanti, Ema; Mustofa
HAYATI Journal of Biosciences Vol. 32 No. 5 (2025): September 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.5.1225-1239

The prevalence of antibiotic-resistant bacteria is increasing every year in Indonesia. This resistance occurs in several antimicrobial categories. A contributing factor to microbial resistance is the capacity of microbes to develop biofilms. Amo.128 is an actinomycete from the Laboratory Biotechnology, BRIN Serpong collection, which is expected to have both antimicrobial and antibiofilm activity. This study aimed to identify amo.128 macroscopically, microscopically, and molecularly; to determine the antibacterial and antibiofilm activity; to identify secondary metabolites; and to understand the mechanism of quorum sensing inhibition by in silico with proteins targeting SdiA and AgrA. Based on macroscopic and microscopic observations, the amo.128 isolate belongs to the genus Streptomyces. Phylogenetic analysis of the amo.128 isolate is 100% similar to Streptomyces parvus strain NBRC 14599. The amo.128 metabolite contains several compounds, including N-acetyltyramine, cyclophenylalanylprolyl/cFP, and the pyrrole-pyrazine group. The MIC/MBC/MIC50 value of the amo.128 metabolite against Staphylococcus aureus is 25/50/28.48 ppm, while for Escherichia coli it is 100/200/49.38 ppm. The amo.128 metabolite reduced biofilms formed by S. aureus and E. coli with BRC50 values of 62.07 ppm and 60.44 ppm, respectively. The amo.128 metabolite compound, cyclophenylalanylprolyl/cFP, has potential activity as a quorum-sensing inhibitor.

Potential active compounds of Streptomyces sennicomposti GMY01 for antiplasmodial and antiSARS-CoV-2 revealed by targeted metabolomic and molecular docking Damayanti, Ema; Nisa, Khoirun; Febriansah , Rifki; Hadi, Ismanurrahman; Dinoto, Achmad; Widada, Jaka; Mustofa
Indonesian Journal of Pharmacology and Therapy Vol 5 No 3 (2024)
Publisher : Faculty of Medicine, Public Health, and Nursing Universitas Gadjah Mada and Indonesian Pharmacologist Association or Ikatan Farmakologi Indonesia (IKAFARI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijpther.9825

Streptomyces sennicomposti GMY01 is a bacterium with huge biotechnological potential that revealed by genome mining analysis. This research aimed to investigate the potential compounds as antiplasmodial and the antiSARS-CoV-2 from the S. sennicomposti GMY01 using targeted metabolomic and in silico molecular docking. The crude extract was obtained by extraction of supernatant from fermentation product of the S. sennicomposti GMY01. The secondary metabolite profiling was obtained by using ultra-high-performance liquid chromatography (UHPLC) coupled to targeted high-performance mass spectrometry (HRMS) based on genome mining data of whole genome sequence (WGS). In silico molecular docking was performed on important target protein of P. falciparum i.e. glutathione reductase (PfGR), lactate dehydrogenase (PfLDH), phosphoethanolamine methyltransferase (Pfpmt), erythrocyte membrane protein 1 (PfEMP1) and glutathione-S-transferase (PfGST); and of SARS-CoV-2 proteins i.e. protease domain, spike glycoprotein, receptor-binding domain angiotensin-converting enzyme 2 (RBD-ACE2), 3-chymotrypsin-like protease (3CLpro), and RNA-dependent RNA polymerase (RdRp). One compound from S. sennicomposti GMY01 extract, albaflavenone was confirmed by targeted LC-HRMS. On molecular docking analysis, albaflavenone showed higher affinity than chloroquine as antiplasmodial drug and exhibited same affinity to remdesivir as antiSARS-CoV-2. Stertomyces sennicomposti GMY01 has promising biotechnological potential for drug development as antiplasmodial and anti-SARS-CoV-2 agent. Further study is needed, especially regarding in vitro testing of albaflavenone as antiplasmodial and antiSARS-CoV2.

The Effect of Fermentation Time on The Quality of Mocaf (Modified Cassava Flour) with Raw Material Bokor Genotype Cassava Prastiwi, Erfa Kurnia; Fatoni, Rois; Fathoni, Ahmad; Setiarto, R. Haryo Bimo; Damayanti, Ema
Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Vol. 13 No. 1 (2024): March 2024
Publisher : The University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jtep-l.v13i1.12-26

Cassava genotype Bokor has a fairly high content of beta carotene, which is beneficial for health and can also support the development of the functional food industry. MOCAF (Modified Cassava Flour) is a product made from cassava flour which is processed by modifying cassava cells through fermentation. This study aims to analyze the effect of long fermentation time on the characteristics of MOCAF from beta-carotene-rich cassava genotype Bokor. In this study, three variations of fermentation time were used, namely 15 hours, 24 hours and 40 hours. The research results showed that the time of fermentation affected the physicochemical properties of MOCAF. NIR analysis showed that the fermentation time affected the chemical composition of MOCAF. At 40 hours (last time of fermentation), there was a decrease in fat content with a value of 1.59% and an increase in water, protein and fibre content with a value of 13.71%, 3.94% and 2.28%, respectively. Proximate analysis showed that there was an effect of the length of fermentation time on the MOCAF content, namely at 40 hours, there was an increase in water content with a value of 10.10%, a decrease in dry matter, ash, protein, fat, fibre with a value of 89.90%, 0.60%, 0.78% respectively, 0.45%, and 1.29%. Scanning Electron Microscope (SEM) analysis shows the effect of fermentation time on the morphological properties of MOCAF starch granules. The longer the fermentation time, the more perforated or damaged the surface of the starch granules. Keywords: Fermentation time, MOCAF flour, NIR (Near Infrared), Proximate, SEM (Scanning Electron Microscope)

The Effect of Drying Method on The Quality of MOCAF (Modified Cassava Flour) from Raw Material Beta-Carotene-Rich Bokor Genotype Cassava Aprilia, Aqsha Putri; Fathoni, Rois; Fathoni, Ahmad; Setiarto, Raden Haryo Bimo; Damayanti, Ema
agriTECH Vol 44, No 2 (2024)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/agritech.84155

The high dependence on flour imports is a significant challenge to overcome by processing local food ingredients through diversification. To address this challenge, the use of MOCAF (Modified Cassava Flour) as a substitute for wheat flour has been carried out in the manufacture of wet and dry noodles, along with analog rice, and bread. The optimization of MOCAF production from beta-carotene-rich cassava depends on the selection of appropriate equipment technology and drying process. Therefore, this study aimed to analyze the effect of drying method on MOCAF characteristics of beta-carotene-rich cassava genotype. The analysis was carried out using two methods, namely sun drying for 3-4 days and oven drying at 60 o C for 24 hours. Parameters analyzed included viscosity, solubility, syneresis, Near Infra-Red analysis, proximate analysis, and Scanning Electron Microscope (SEM). The results showed that drying affected the physicochemical properties of MOCAF, including viscosity, solubility, and syneresis. NIR (Near-infrared) and proximate analysis showed that drying process affected the decrease in moisture, ash, protein, and fat content, along with an increase in crude fiber, and dry fiber matter content. Furthermore, SEM microstructural analysis resulted in the reformation of starch granules, characterized by changes in morphology and structure, such as the separation of irregular spherical shapes, and hollowness.

Antiplasmodial activity and genome mining study of marine-derived Streptomyces sp. GMY01 Damayanti, Ema; Widada, Jaka; Dinoto, Achmad; Mustofa
Indonesian Journal of Pharmacology and Therapy Vol 2 No 2 (2021)
Publisher : Faculty of Medicine, Public Health, and Nursing Universitas Gadjah Mada and Indonesian Pharmacologist Association or Ikatan Farmakologi Indonesia (IKAFARI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijpther.1335

High resistance to chloroquine in most malaria-endemic area in the world leads to the need for new antimalaria drugs. Marine bacterium Streptomyces is the source for potential new antimalarial molecules. This research aimed to investigate antiplasmodial activity of marine-derived of Streptomyces sp. GMY01 and to identify potential active compounds using genome mining study. In vitro antiplasmodial activity assays using flow cytometry method showed that the ethyl acetate extract of this bacterium had high antiplasmodial activity (IC50 value of 1.183 µg/mL) on Plasmodium falciparum FCR3. Genome mining analysis of whole-genome sequences using antiSMASH 6.0 beta version revealed that Streptomyces sp. GMY01 had 28 biosynthetic gene clusters (BGCs), including the genes encoding polyketide synthase, non-ribosomal peptide synthetase, terpene, lanthipeptide, bacteriocin, butyrolactone, ectoin, siderophore, and others. The known BGCs were predicted to be involved in the production of known compounds from gene clusters ranged from 5 to 100% similarity. Ongoing purification and elucidation of the structures will allow identification of the active compounds produced by marine-derived Streptomyces sp. GMY01.

Co-Authors Achmad Dinoto Ahmad Fathoni Aprilia, Aqsha Putri Ash Shabah, Musyaffa Amin Fathoni, Rois Febriansah , Rifki Hadi, Ismanurrahman Hardi Julendra Hayati, Septi Nur Hendra Herdian Heru Agus Cahyanto, Heru Agus JAKA WIDADA Khoirun Nisa Lies Mira Yusiati Lusty Istiqomah Mudinillah, Adam Mustofa Mustofa Prastiwi, Erfa Kurnia R. Haryo Bimo Setiarto, R. Haryo Bimo Raden Haryo Bimo Setiarto, Raden Haryo Bimo Rafika Sari Rofiq Sunaryanto Rois Fatoni Suprihatin Suprihatin Sylvia Utami Tunjung Pratiwi Tri Untari Yosi Bayu Murti

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