Lince Mukkun
Agro Technology Department, Faculty Of Agriculture, Universitas Nusa Cendana, Kupang, NTT, Indonesia

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Dampak Residu Pestisida Terhadap Keanekaragaman Jamur Tanah Pada Lahan Sayuran: Impact of Pesticide Residues on the Diversity of Soil Fungi on Vegetable Land Martha Maria Magdalena Benu; Anthonius S. J. Adutae; Lince Mukkun
Jurnal Ilmu Tanah dan Lingkungan Vol 22 No 2 (2020): Jurnal Ilmu Tanah dan Lingkungan
Publisher : Departemen Ilmu Tanah dan Sumberdaya Lahan, Fakultas Pertanian, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jitl.22.2.80-88

Abstract

Insecticides have a negative effect on soil fungi because they contain dangerous toxic compounds. The use of insecticides by Noelbaki village farmers in vegetable cultivation is quite intensive with, a frequency of 2-3 spraying per week. The application of this insecticide is carried out continuously during the planting of vegetables so that it has a negative impact on the soil environment. This study aimed to determine the content of insecticide residues and the diversity of soil fungi in mustard vegetable fields. The research method used was a survey method conducted on several mustard vegetable farmers by collecting information using a questionnaire as a means of collection data and laboratory tests of residue content in the soil. Soil samples were taken using a diagonal pattern with 5 replications on mustard greens on Vertisols and Inceptisols both applied and without insecticides. Soil samples in a composite soil were carried out for the analysis of residual content and physical and chemical properties of the soil, while not composist was used for the observation of soil fungi. The variables observed were the content of insecticide residues, population density, diversity and frequency of presence, soil fungi and the physical and chemical properties of soil on the vegetable land. Analysis of insecticide residues was carried out using the Gas Chromatography-Spectrometry Mass (GC-MS/ MS) method, and the counting of microbes was carried out using the Plate Counting Agar (PCA) method, while the identification of microbes was carried out by microscopic and macroscopic observations. Insecticide residues with the active ingredient Lamda-cihalotrin in the Vertisol type soil samples treated with insecticides were 0.060 ppm, and in the Inceptisol soil samples with the active ingredient Dimetoat as much as 0.042 ppm. The density of soil mushroom population was higher in vegetable fields without the application of Insecticides as much as 71.6 CFU g-1, the value of soil mushroom diversity index was higher in insecticide application vegetable fields 1.609, the frequency of presence of the fungus species Penicillium constant 100% and Mucor 90%.
Initial Moisture Content of Corncobs Plays an Important Role in Maintaining its Quality during Storage Lince Mukkun; Herianus J.D. Lalel; Yuliana Tandirubak
agriTECH Vol 38, No 2 (2018)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (391.488 KB) | DOI: 10.22146/agritech.26663

Abstract

Maize is one of the important staple foods for people in Timor, East Nusa Tenggara Province, Indonesia. Subsistent farmers store the maize for their own consumption until the next harvest season, for seed and feed.  However, high initial water content of the kernel due to improper drying prior storage initiate serious damage and losses during the maize storage.  High water content promotes the growth of fungi and insects, and increase respiration rate, resulting in rapid deterioration of maize. The purpose of this study was to determine the initial moisture content that might minimize damage and losses of maize in the farmers’ storage, and to study the effects of some plant materials that are used to smoke corns before storage. The experiment was initiated by sun-drying the harvested corncobs for 0, 2, 4, 6, 8, and 10 days (6 hours a day). This experiment was designed using Completely Randomized Design with 6 treatments and 3 replications. Dried corncobs were stored in the farmer’s storage for 4 months. The effects of maize kernels’ initial water content on the development of water content in kernels; the percentage of damaged kernels; and the species of pathogen and insects were investigated during storage with 2-week intervals.  The results demonstrated that drying the corncobs prior storage for 10 days, resulting in 12.96% of water content, significantly decreased the percentage of seed damage to 6.5%, as compared to without drying process which resulted  in 63%.  Aspergillus flavus, Fusarium sp., and Penicillium sp were found to be the main pathogen during storage.  There are no insect pests found during the storage. 
Dampak Residu Pestisida Terhadap Kepadatan Dan Keanekaragaman Jamur Tanah Pada Lahan Sayuran Martha Benu; A.S.J Adutae; Lince Mukkun
Bumi Lestari Journal of Environment Vol 19 No 2 (2019)
Publisher : Environmental Research Center (PPLH) of Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/blje.2019.v19.i02.p03

Abstract

The use of pesticides in agricultural soils in addition to a positive impact because it has a phytoponic effect for plants, also cause negative impacts on the environment. Pesticide residues in agricultural soils may cause disruption to the growth and diversity of useful microorganisms in the soil such as soil fungi. Fungi play an important role in nutrient cycling, disease control, binding of soil particles and as a remodel in the soil web chain. This research was conducted in farmer's land of Noelbaki village, Kupang Tengah sub-district, Kupang regency. The purpose of this research is to know the residue content of pesticide, population density and soil fungi diversity on vegetable land applied by pesticide and without pesticide. The research method is survey and sampling of soil on vegetable land application of pesticide and without pesticide soil type Vertisol and Inceptisol. The pesticide used as reference for test of active ingredient profenofos 500 g / l, lamda-cilhalotrin 106 g / l, permethrin 20.04 g / l, carbosulfan 200,11 g / l, dimetoate 400 g / l, mankozeb 80% and propineb 70% from classes of pyrethroids, carbamates and organophosphates. Analysis of pesticide residues on soil samples of soil pesticides Vertisol and Inceptisol detected residues of Lamda-cihalotrin and Dimethoat with concentrations of 0.060 ppm and 0.042 ppm respectively. Pesticide residue in the soil is below the BMR of 0.10 ppm. Population density analysis of Vertisol and Inceptisol soil type soil samples respectively (NV-1) 71.6 cfu g-1 and (NI-1) 21.6 cfu g-1 and without pesticides respectively (NV-2) 16.6 cfu g-1 and (NI-2) 29.2 cfu g-1. The analysis of soil pesticide soil diversity of Vertisol and Inceptisol soils respectively (NV-1) 1,608 (NI-1) 1,579 and without pesticide respectively (NV-2) 1.584 and (NI-2) 1,595. Result of analysis of medium soil fungi diversity where H> 1. The types of fungi that are identified are Penicillium, Aspergillus niger, Aspergillus candidus, Aspergilus flavus, Aspergilus nidulans, Fusarium, Trichoderma, and Mucor.
INVENTARISASI HAMA PASCAPANEN JAGUNG DALAM PENYIMPANAN DI TINGKAT PETANI DAN PEDAGANG DI KUPANG Yustina Maria Silvia Wonga Puu; Samuel Pakan; Lince Makkun
AGRICA Vol. 1 No. 1 (2008): June
Publisher : Agriculture Faculty of Flores University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37478/agr.v1i1.478

Abstract

This research was conducted at pest and plant disease laboratory, faculty of agriculture Nusa Cendana University in Kupang. Corn sample is taken from fanner in Amarasi, Kupang Timur, Kupang Barat and from the trader in Oesapa, Oesao, Penfui. This research intends to inventory pest after yields corn and to know the damage level of corn in farmer and trader. This research used a 32 kg corn sample for quotation methods. The observed variable is pest insect species, imago population, damage seed percentage, Weight decrease percentage, water level, temperature and moisturizer. Pest insect population and species observed on 1 kg corn. While seed damage and weight decrease per cent on 100 g corn seed and 5 g for water level. Data result tabulated and descriptively analyzed. The result of the research showed 1 pest insect species in the farmer corn storage is S.zeanmys and 2 species in trader corn storage is S.zeanmys and T confusum. Highest population S.zeamays founded in Penfui trader by 50,50/kg and lowest population 4,50/kg in farmer at Amarasi. T.confusum population only founded in trader not in farmer level. Highest population 2,17/kg in Penfui trader and lowest 0,17/kg in Oesapa trader. While highest seed damage percentage founded in Penfui trader by 11,13% and lowest 1,22% in farmer at Amarasi and highest weight decrease percentage founded in Oesapa trade by 0,77% and lowest 0,25% in farmer at Kupang Barat
Bioactive Secondary Metabolites from the Mangrove Endophytic Fungi Nigrospora oryzae Antonius Rolling Basa Ola; Titus Lapailaka; Hermania Em Wogo; Julinda Bendalina Dengga Henuk; Agnes Simamora; Lince Mukkun; Peter Proksch; Chong Dat Pham
Indonesian Journal of Chemistry Vol 21, No 4 (2021)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.63129

Abstract

Mangrove forest has a distinctive habitat adapting with marine and terrestrial environment. Chemical investigation of the extract from mangrove endophytic fungi Nigrospora oryzae had resulted in the isolation of sterigmatocystin (1) and pestalopyrone (2). The structure of sterigmatocystin (1) and pestalopyrone (2) were elucidated using mass, UV and NMR spectrometers together with the comparison with the literature data. The study also showed that sterigmatocystin displayed moderate cytotoxicity but it could be further developed as antiviral and antibacterial agent based on the SAR information reported from its analogue and derivatives.