Article Per Year (5 Year)
p-Index From 2021 - 2026
0
P-Index
This Author published in this journals
All Journal INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY
Nastasya Nunki
Department of Medical Laboratory Technology, Faculty of Health, Universitas Nahdlatul Ulama Surabaya, Surabaya, Indonesia
Health Professions Social Sciences

Published : 1 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 1 Documents
Search

 1 
PURPLE SWEET POTATO (Ipomoea batatas L.) PEELS EXTRACT AS AN ALTERNATIVE DYE FOR BACTERIA GRAM STAINING Nastasya Nunki; Diah Titik Mutiarawati; Endah Prayekti
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 2 No 2 (2020): Increasing Research in Diagnostic Laboratory Testing
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v2i2.1655

Abstract

Crystal violet and Safranin are dyes in Gram staining, which are carcinogenic. Alternative safe materials are needed to minimize the use of carcinogenic properties. Purple sweet potato (Ipomoea batatas L.) peels were the candidate of the alternative dye source because of its high anthocyanin pigment. The purpose of this study was to determine purple sweet potato (Ipomoea batatas L.) peels extract as an alternative to Gentian violet in Gram staining of bacteria. Extracts obtained from purple sweet potato peels studied with varying concentrations of 50%, 60%, and 75% for 1, 3, and 5 min as a substitute for Gentian violet on Bacillus sp. The parameters observed from this study based on visual field clarity, glass slide cleanliness, contrast, bacterial shape, bacterial colour. Each extract concentration compared with a control group using Gentian violet. The results showed that optimum staining in 50% concentration for 5 min, 60% concentration for 5 min, 75% concentration for 3 min, and 5 min respectively. The present study exhibited the potency of Ipomoea batatas L. peels extract as an alternative staining agent.
Co-Authors Mutiarawati, Diah Titik Prayekti, Endah