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All Journal Jurnal Penelitian Kesehatan Suara Forikes Health Notions THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Manuju : Malahayati Nursing Journal 2-TRIK: TUNAS-TUNAS RISET KESEHATAN Jurnal Analis Medika Biosains (JAMBS) INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Jurnal Ilmiah Tatengkorang Berkala Penelitian Hayati Jurnal Pengabdian Kepada Masyarakat Mulawarman Analis Kesehatan Sains (e-Journal) Jurnal Rakat Sehat : Pengabdian Kepada Masyarakat

Mutiarawati, Diah Titik

Poltekkes Kemenkes Surabaya

Author-ID : 1559707

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Dentistry Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary

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Liquid Chitosan from Corbula faba Hinds on Soil Transmitted Helminth (STH) in Pakcoy (Brassica chinensis L.) Diah Titik Mutiarawati
Health Notions Vol 6, No 4 (2022): April
Publisher : Humanistic Network for Science and Technology (HNST)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33846/hn60407

Worms are a health problem that is still commonly found in developing countries. One of the causes of helminthiasis is Soil-Transmitted Helminth (STH). The worms that cause worm problems are Ascaris lumbricoides, Trichuris trichiura, Hookworm (Necator americanus and Ancylostoma duodenale). Chitosan has been discovered to have a quality positively charged polycationic, leading to changes in cell permeability. Chitosan can be found in the framework of Crustacea sp., such as white mussel shell. The purpose of this research was to determine the effectiveness of liquid chitosan from white mussel shells (Corbula faba Hinds) against Soil-Transmitted Helminth on Pakcoy (Brassica chinensis L.). The research results at a concentration of 2% on average worm larvae death time of 5.25 minutes, 1.5% concentration on average worm death time of 5.15 minutes, the concentration of 1% and 0.5% did not occur death of worm larvae. There is a significant difference in the exposure of liquid chitosan from white shell to death time of worm larvae. These results show that the liquid chitosan from white mussel shell to Soil-Transmitted Helminth on mustard meat is effective at a concentration of 1.5% with an average death time of 5.15 minutes. Keywords: Soil-Transmitted Helminth; chitosan; Corbula faba hinds

Phytochemical Screening and In Vitro Antibacterial Activity of Green Tea (Camellia Sinensis L) Extract Against Staphylococcus Epidermidis Hardian Bimanto; Yuyun Dwi Wahyuni; Diah Titik Mutiarawati; Lully Hanni Endarini
Health Notions Vol 4, No 8 (2020): August
Publisher : Humanistic Network for Science and Technology (HNST)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33846/hn40805

This study aimed to determine phytochemical screening and ethanol extract activity of the green leaves against Staphylococcus epidermidis. The microbial activity of the ethanol extract was carried out using the diffusion and dilution method with concentrations used of 1.5%, 2.5%, 3.5%, 4.5%, and 5.5% accompanied by three repetitions. The results showed that the ethanol extract of green tea leaves contained alkaloids, saponins, steroids or triterpenoids, flavonoids, polyphenols, and tannins and had the largest inhibition zone diameter of 30mm at a concentration of 4.5%. KHM (Minimum Inhibitory Content) at a level of 4.5% and KBM (Minimum Kill Rate) at a concentration of 5.5%. Analysis of the data with the normality test then proceed with the Kruskal Wallis test obtained a value of P = 0.008. With the value P

Rice Bran as Opportunity Media for Candida albicans Increase Diah Titik Mutiarawati
Health Notions Vol 5, No 12 (2021): December
Publisher : Humanistic Network for Science and Technology (HNST)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33846/hn51205

The incidence of invasive fungal infections caused by fungi of the genus Candida is reported to be increasing in various countries. C. albicans can be isolated and cultured in liquid media, one of which is PDB (Potato Dextrose Broth), a common medium used for the growth of yeasts and moulds. Bekatul or rice bran is a waste from rice milling, which still contains nutrients such as carbohydrates and protein, which are needed as energy sources for mushroom growth. Colonies were C. albicans grown in bran liquid media at mass concentrations of 20 grams, 40 grams, 60 grams, 80 grams and 100 grams with PDB as the gold standard. The colonies C. albicans growing were then confirmed by planting on PDA media (Potato Dextrose Agar), and microscopic testing was performed. The results showed that C. albicans grew well in bran liquid medium at pH 5.5-5.0, which was incubated for 1-5 days at a temperature of 25°C-30°C and was characterized by the growth of white colonies on the bottom of the tube, while the number of C. albicans that grew on liquid bran media was more than that of PDB media. The conclusion of this research is that the liquid bran media can be used as an alternative medium for PDB for the growth of the fungus C. albicans.Keywords: white rice bran; Candida albicans; alternative media; potato dextrose broth

In Vitro Anthelmintic Activity of Acalypha Indica Leaves Extracts Diah Titik Mutiarawati
Health Notions Vol 4, No 3 (2020): March
Publisher : Humanistic Network for Science and Technology (HNST)

Anting-anting leaves (Acalypha indica L) is one of the wild plants (weeds) that are often used by the community to treat helminthic diseases such as ascariasis, containing several potentially anthelmintic compounds, namely saponins, tannins and flavonoids. The purpose of this study was to determine the effect of giving Anting-anting leaf extract (Acalypha indica L) as an anthelmintic to the time of death of pork roundworms (Ascaris suum Goeze). The method in this study was pre-experimental with a post test only group design. The subjects of the study were Ascaris suum. The study was conducted at the Parasitology Laboratory of the Surabaya Polytechnic Health Medical Laboratory Technologist Department in December-May 2018. This study used 5 treatment groups, 0.9% NaCl as negative control and 0.25% pirantel pamoate as positive control and 60%, 80% and 100%. Data were analyzed using the Kolmogrov-Smirnov test, then the Kruskal-Wallis test was then continued using a Post-Hoc test to determine the difference in anthelmintic power of ear leaf extract of each concentration with positive control over the time of worm death. The average time of Ascaris suum worm death at a concentration of a concentration of 60% for 228.5 minutes, a concentration of 80% for 174 minutes, and a concentration of 100% for 92 minutes. So it can be concluded that the leaf extract of Acalypha L has an anthelmintic effect on the Ascaris suum worm. Keywords: anthelmintic; Ascaris suum; anting-anting leaf

PURPLE SWEET POTATO (Ipomoea batatas L.) PEELS EXTRACT AS AN ALTERNATIVE DYE FOR BACTERIA GRAM STAINING Nastasya Nunki; Diah Titik Mutiarawati; Endah Prayekti
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 2 No 2 (2020): Increasing Research in Diagnostic Laboratory Testing
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v2i2.1655

Crystal violet and Safranin are dyes in Gram staining, which are carcinogenic. Alternative safe materials are needed to minimize the use of carcinogenic properties. Purple sweet potato (Ipomoea batatas L.) peels were the candidate of the alternative dye source because of its high anthocyanin pigment. The purpose of this study was to determine purple sweet potato (Ipomoea batatas L.) peels extract as an alternative to Gentian violet in Gram staining of bacteria. Extracts obtained from purple sweet potato peels studied with varying concentrations of 50%, 60%, and 75% for 1, 3, and 5 min as a substitute for Gentian violet on Bacillus sp. The parameters observed from this study based on visual field clarity, glass slide cleanliness, contrast, bacterial shape, bacterial colour. Each extract concentration compared with a control group using Gentian violet. The results showed that optimum staining in 50% concentration for 5 min, 60% concentration for 5 min, 75% concentration for 3 min, and 5 min respectively. The present study exhibited the potency of Ipomoea batatas L. peels extract as an alternative staining agent.

PENGARUH ASAM ASKORBAT TERHADAP KADAR TIMBAL FETUS DAN AKTIVITAS ENZIM SITOKROM P450 1A1 (CYP1A1) PADA INDUK MENCIT TERINTOKSIKASI TIMBAL Juliana Christyaningsih; Harianto Notopuro; Win Darmanto; Diah Titik Mutiarawati
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 16 No 1 (2010): December 2010
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/280

Lead contamination occurs through air pollution and industry, enter the body through respiratory and digestive tract. High lead content will accumulate and affect adversely the cognitive function, causing neuropsychological dysfunction, encephalopathy, hyperactivity and other problems in children, disrupt the central nervous system and the immune system of children as well. This experimental research was randomized control group post-test only design. The experiment used of 27 pregnant mice, divided into three groups: negative control group, which were given distilled water, positive control group were exposed only to lead and the third group were exposed to lead and administered ascorbic acid. 25 mg/kg/day/orally neutral lead acetate was given during gestation day 7 to 16, and ascorbic acid 64 mg/kg/day/orally, started on gestation day 9 to 16. Treatment group with ascorbic acid supplementation had the lowest CYP1A1 enzyme activity compared to positive and negative control groups. This results confirmed by the molecular weight of CYP1A1 enzyme ranges 53.7 to 59.2 kDa, and the western blotting test showed the same thin band both two groups. The lowest of the average lead concentration in the head of fetal mice was found on the group of mice that treated with vitamin C. Supplementation of ascorbic acid can protect the liver and fetuses, by suspected mechanism that ascorbic acid could chelate the lead and excrete it via urine.

Aktivitas Antibakteri Ekstrak Daun Teh Hijau (Camellia sinensis) Terhadap Pertumbuhan Bakteri Escherichia coli Lully Hanni Endarini; Diah Titik Mutiarawati
2-TRIK: TUNAS-TUNAS RISET KESEHATAN Vol 11, No 3 (2021): Agustus 2021
Publisher : FORUM ILMIAH KESEHATAN

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33846/2trik11308

Green tea leaves (C. Sinensis) can be used as a treatment for bacterial infections because they contain antibacterial active compounds. One of the bacteria that causes diarrhea is Escherichia Coli. Green tea (Camellia sinensis) is known as a plant that has antibacterial activity. The purpose of this study was to determine the optimization of the inhibitory power of green tea leaf extract (Camellia sinensis) against the growth of Escherichia coli bacteria. This research is an experimental laboratory conducted at the Bacteriology Laboratory, Department of Medical Laboratory Technology, Surabaya April 2021. This study uses the disk diffusion method Kirby Bauer, namely Mueller Hinton Agar (MHA) media which has been planted with Escherichia coli bacteria in disks that have been soaked in green tea leaf extract (Camellia sinensis) at concentrations of 25%, 50%, 75%, and 100% and then incubated for 24 hours in an incubator. From the results of the study, it can be concluded that green tea leaf extract (Camellia sinensis) has antibacterial activity. The optimal results in this study were Escherichia coli bacteria at a concentration of 25% with an average diameter of 10.25 mm This is because the main content of green tea leaves (Camellia sinensis) is catechins and alkaloids as strong antibacterials.Keywords: antibacterial; camellia sinensis; Escherichia coliABSTRAK Daun teh hijau (C.sinensis) dapat digunakan sebagai pengobatan infeksi bakteri karena mengandung senyawa aktif antibakteri. Salah satu bakteri penyebab diare adalah Escherichia Coli . Teh hijau (Camellia sinensis) dikenal sebagai tanaman yang mempunyai aktivitas antibakteri. Tujuan penelitian ini adalah untuk mengetahui optimalisasi daya hambat ekstrak daun teh hijau (Camellia sinensis) terhadap pertumbuhan bakteri Escherichia coli.Penelitian ini bersifat eksperimental laboratorium yang dilakukan di Laboratorium Bakteriologi Jurusan Teknologi Laboratorium Medik Surabaya April 2021. Penelitian ini menggunakan disk diffusion metode Kirby Bauer, yaitu media Mueller Hinton Agar (MHA) yang telah ditanami biakan bakteri Escherichia coli di dalam disk yang sudah di rendam ekstrak daun teh hijau (Camellia sinensis) konsentrasi 25%, 50%, 75%, dan 100% lalu diinkubasi selama 24 jam pada inkubator. Dari hasil penelitian dapat disimpulkan bahwa esktrak daun teh hijau (Camellia sinensis) mempunyai aktivitas antibakteri. Hasil optimal pada penilitian ini bakteri Escherichia coli pada konsentrasi 25% dengan rata-rata diameter 10,25 mm Hal tersebut dikarenakan kandungan utama daun teh hijau (Camellia sinensis) katekin dan alkaloid sebagai antibakteri yang kuatKata kunci: antibakteri; camellia sinensis; Escherichia coli

Uji Efektivitas Ekstrak Daun Sirih Hijau (Piper Betle L), Daun Sirih Merah (Piper Crocatum) dan Daun Sirih Kuning (Piper Betle) terhadap Mortalitas Larva Nyamuk Aedes Aegypti Heni Nuril Arfiyanti; Diah Titik Mutiarawati; Retno Sasongkowati; Wisnu Isntanto
Malahayati Nursing Journal Vol 4, No 10 (2022): Volume 4 Nomor 10 2022
Publisher : Universitas Malahayati Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33024/mnj.v4i10.7510

ABSTRACT The Aedes aegypti mosquito is the main vector of DHF (Dengue Hemorrhagic Fever). In the city of Surabaya in 2019, there were 277 cases of dengue fever. Control using chemical insecticides can create resistance for larvae so that other methods can be used, namely herbal insecticides on green betel leaves, red betel leaves and yellow betel leaves which contain compounds such as essential oils, alkaloids, flavonoids, saponins and tannins which are toxic and can affect the digestive system. and nervous system in Aedes aegypti larvae. The purpose of this study was to determine the effectiveness of green betel leaf extract (Piper betle L), red betel leaf (Piper crocatum) and yellow betel leaf (Piper betle) as natural insecticides against Aedes aegypti larvae. This research was conducted at the Entomology Laboratory of the East Java Provincial Health Office in May 2022. The data collection technique was experimental. In this study there were 5 treatment groups with concentrations of 0.1%, 0.5% and 1% and there were positive control groups (abate) and negative control groups, each treatment had 5 replications. Each test contained 20 instar III Aedes aegypti larvae. With the duration of exposure for 60, 120, 180, 240, 300, 360, 420, 480, 540, 1440. The results of this study indicate that the greater the concentration and duration of exposure, the more mortality of mosquito larvae. The results of the data analysis of this study showed that they were not normally distributed and were not homogeneous, so the Kruskal-Wallis test gave a p value < (0.05) indicating the effect of the extract on the concentration. Then proceed to the Post-Hoc test showing different results at each concentration, if p> (0.05) then there is no difference, if p < (0.05) then there is a difference in each extract concentration on larval mortality. Keywords: Aedes Aegypti, Piper Betle L, Piper Crocatum, Piper Betle, Larva Mortality ABSTRAK Nyamuk Aedes aegypti merupakan vektor utama dari penyakit DBD (Demam Berdarah Dengue). Pada kota Surabaya tahun 2019 kasus DBD dengan jumlah 277 kasus. Pengendalian menggunakan insektisida kimia dapat menjadikan resistensi untuk larva sehingga dapat menggunakan cara lain yaitu insektisida herbal pada daun sirih hijau, sirih merah dan sirih kuning yang memiliki kandungan senyawa seperti minyak atsiri, alkaloid, flavonoid, saponin dan tanin yang bersifat sebagai racun dapat mempengaruhi sistem pencernaan dan sistem saraf pada larva Aedes aegypti. Tujuan penelitian ini untuk mengetahui efektivitas ekstrak daun sirih hijau (Piper betle L), sirih merah (Piper crocatum) dan sirih kuning (Piper betle) sebagai insektisida alami terhadap larva Aedes aegypti. Penelitian ini dilaksanakan di LaboratoriumEntomologi Dinas Kesehatan Provinsi Jawa Timur pada bulan Mei 2022. Teknik pengumpulan data secara eksperimental. Penelitian ini terdapat 5 kelompok perlakuan konsentrasi 0,1%, 0,5% dan 1% serta terdapat kelompok kontrol positif (abate) dan kontrol negatif, setiap perlakuan terdapat 5 kali replikasi. Setiap uji berisi 20 larva Aedes aegypti instar III. Dengan lama pemaparan selama 60, 120, 180, 240, 300, 360, 420, 480, 540, 1440. Hasil penelitian ini menunjukkan bahwa semakin besar konsentrasi dan lama pemaparan maka semakin banyak mortalitas larva nyamuk. Hasil analisa data penelitian ini menunjukkan tidak berdistribusi normal dan tidak homogen sehingga dilakukan uji kruskal-wallis memberikan nilai p < (0,05) menunjukkan adanya pengaruh ekstrak pada konsentrasi. Kemudian dilanjutkan ke uji Post-Hoc menunjukkan hasil berbeda – beda pada setiap konsentrasi, apabila p > (0,05) maka tidak ada perbedaan, apabila p < (0,05) maka adanya perbedaan pada setiap konsentrasi ekstrak terhadap mortalitas larva. Kata Kunci: Aedes Aegypti, Piper Betle L, Piper Crocatum, Piper Betle, Mortalitas Larva

Efektivitas Berbagai Ekstrak Varietas Daun Sirih sebagai Repelen Daya Tolak terhadap Gigitan Nyamuk Aedes Aegypti Vemy Rahmany Kurniawan; Diah Titik Mutiarawati; Lully Hanni Endarini; Retno Sasongkowati
Malahayati Nursing Journal Vol 4, No 10 (2022): Volume 4 Nomor 10 2022
Publisher : Universitas Malahayati Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33024/mnj.v4i10.7511

ABSTRACT DHF is a disease caused by the dengue virus and is transmitted from person to person through the bite of the Aedes aegypti mosquito. Control efforts can be carried out using natural insecticides from plants, namely betel leaf. Betel leaf contains saponins, flavonoids, tannins, eugenol, kavicol, alkaloids and essential oils. This study aims to determine the effectiveness of green betel leaf extract (Piper betle L), yellow betel leaf (Piper betle) and red betel leaf (Piper crocatum) as repellant for Aedes aegypti mosquitoes. The research was carried out at the Entomology Laboratory of the East Java Provincial Health Office in April 2022. The data collection technique was carried out experimentally. Preparation of spray concentration 10%, 15%, 17%, 20% was sprayed on the back of the hand exposed to 25 female Aedes aegypti mosquitoes, the exposure was carried out for 5 minutes within 6 hours with every 1 hour the hand was reinserted. This research was conducted with 4 times of replication. The results showed that the highest percentage of repelling repellant occurred at a concentration of 20%, namely in green betel (Piper betle L.) by 95%, yellow betel (Piper betle) by 94%, and red betel (Piper crocatum) by 91%. The results of the study were analysis of alternative test data, namely Kruskall Wallis because the data were not homogeneously distributed, then continued with the Post Hoc test to find out which were the significant differences between the treatment groups. The value of the Kruskall Wallis test was obtained, the value of p < (0.05) was Sig. 0.000 which means there is a difference in repulsion to the Aedes aegypti mosquito. Keywords: Piper betle L, Piper betle, Piper crocatum, Aedes aegypti, Repellant ABSTRAK DBD adalah penyakit yang disebabkan virus dengue dan ditularkan dari orang ke orang melalui gigitan nyamuk Aedes aegypti. Upaya pengendalian dapat dilakukan menggunakan insektisida alami dari tanaman yaitu daun sirih. Daun sirih mengandung senyawa saponin, flavonoid, tanin, eugenol, kavicol, alkaloid dan minyak atsiri. Penelitian ini bertujuan mengetahui efektivitas ekstrak daun sirih hijau (Piper betle L), sirih kuning (Piper betle) dan sirih merah (Piper crocatum) sebagai repelen daya tolak nyamuk Aedes aegypti. Penelitian dilaksanakan di Laboratorium Entomologi Dinas Kesehatan Provinsi Jawa Timur pada bulan April 2022. Teknik pengumpulan data yang dilakukan secara eksperimental. Sediaan spray konsentrasi 10%,15%,17%,20% disemprotkan pada punggung tangan yang dipaparkan 25 ekor nyamuk betina Aedes aegypti, pemaparan dilakukan selama 5 menit dalam waktu 6 jam dengan setiap 1 jam sekali tangan dimasukkan kembali. Penelitian ini dilakukan dengan 4 kali replikasi. Hasil penelitian didapatkan persentase repelen daya tolak tertinggi terjadi pada konsentrasi 20% yaitu pada sirih hijau (Piper betle L.) sebesar 95%, sirih kuning (Piper betle) sebesar 94%, dan sirih merah (Piper crocatum) sebesar 91%. Hasil penelitian dilakukan analisa data uji alternatif yaitu Kruskall wallis karena data tidak berdistribusi homogen kemudian dilanjutkan dengan uji Post Hoc untuk mengetahui mana saja perbedaan yang signifikan antar kelompok perlakuan. Didapatkan nilai uji Kruskall wallis nilai p < (0,05) yaitu Sig. 0,000 yang mengartikan adanya perbedaan daya tolak terhadap nyamuk Aedes aegypti. Kata Kunci: Piper betle L, Piper betle, Piper crocatum, Aedes aegypti, Repelen

Pertumbuhan Bakteri Bacillus Cereus dan Salmonella Typhi pada Ekstrak Mentimun (Cucumis Sativus) Lully Hanni Endarini; Diah Titik Mutiarawati; Alda Nugrahini
Jurnal Penelitian Kesehatan SUARA FORIKES Vol 13, No 3 (2022): Juli 2022
Publisher : FORIKES

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33846/sf13317

Enteropathy caused by ingestion of food contaminated with bacteria causes morbidity and mortality in many countries. Several bacteria, including Salmonella typhi and Bacillus cereus have been reported to cause food poisoning. Cucumis sativus is useful for humans as antibacterial, hepatoprotective, antioxidant, anthelmintic, antiulcer and anti-inflammatory. Antibacterial test of cucumber ethanol extract was carried out with agar disc-diffusion according to the Kirby Bauer method. The ready test bacterial suspension was then inoculated and put into an incubator at 37°C for 24 hours in an inverted position. After 24 hours, the antibacterial activity around the disc in the test dish was observed by looking at the clear zone around the disc. The results showed that the inhibition zone formed on Salmonella typhi bacteria with concentrations of 30%, 50% and 70% had an average of 11.4 mm, 15 mm and 18.5 mm. Meanwhile, Bacillus cereus bacteria have an average inhibition zone of 7 mm, 18.7 mm and 19.1 mm. The results of the one way ANOVA test obtained a sig value. p<0.05 which proves the presence of antibacterial effect in inhibiting the growth of Salmonella typhi and Bacillus cereus.Keywords: cucumber extract; Salmonella typhi; Bacillus cereus; antibacterial ABSTRAK Enteropati yang disebabkan oleh konsumsi makanan yang terkontaminasi bakteri menyebabkan morbiditas dan mortalitas di banyak negara. Beberapa bakteri, termasuk Salmonella typhi dan Bacillus cereus telah dilaporkan menyebabkan keracunan makanan. Cucumis sativus bermanfaat bagi manusia sebagai antibakteri, hepatoprotektif, antioksidan, anthelmintik, antiulkus dan antiinflamasi. Uji antibakteri ekstrak etanol mentimun dilakukan dengan agar disc-diffusion menurut metode Kirby Bauer. Suspensi bakteri uji yang telah siap kemudian diinokulasi dan dimasukan ke dalam inkubator dengan suhu 37OC selama 24 jam dengan posisi terbalik. Setelah 24 jam, aktivitas antibakteri disekitar cakram dalam cawan uji diamati dengan melihat adanya clear zone di sekitar cakram. Hasil penelitian menunjukkan zona hambat terbentuk pada bakteri Salmonella typhi dengan konsentrasi 30%, 50% dan 70% memiliki rerata 11,4 mm, 15 mm dan 18,5 mm. Sedangkan pada bakteri Bacillus cereus memiliki rerata zona hambat 7 mm, 18,7 mm dan 19,1 mm. Hasil uji one way ANOVA didapatkan nilai sig. p<0,05 yang membuktikan terdapatnya efek antibakteri dalam menghambat pertumbuhan Salmonella typhi dan Bacillus cereus.Kata kunci: ekstrak mentimun; Salmonella typhi; Bacillus cereus; antibakteri

Co-Authors ., Pestariati Alda Nugrahini Anita Dwi Anggraini Anita Dwi Anggraini Astuti Setiyani Bambang Hadi Sugito Darjati Dedi Kurniawan Della Ika Putri Damayanti Demes Nurmayanti Endah Astuti, Sri Sulami Endarini, Lully Hani Endarini, Lully Hanni Era Fitria Yunita Evi Yunita N Evy Diah Woelansari Hadi Suryono Hardian Bimanto Harianto Notopuro Heni Nuril Arfiyanti Ilma Ilma Indah Lestari, Indah Ira Puspita Sari Isfentiani, Dina Istanto, Wisnu Juliana Christyaningsih Junaedi, Winda Rachmawati Khambali, Khambali Kiaonarni OW Lembunai Tat Alberta, Lembunai Tat Luthfi Rusyadi Mamik Mamik Minarti Minarti Muhammad Sholakhuddin Al-Ayubi Museyaroh, Museyaroh N. S. Widodo Nastasya Nunki Nur Hatijah Pengge, Nuning Marina Prayekti, Endah Puspita, Ria Puspitadewi, Teresia Retna Rahayuningsih, Christ Kartika Retno Sasongkowati Retno Sasongkowati Salsyabillah Ramadhani HD. Widya Sasongkowati, Retno Siagian, Hotmaida Siti Alfiah Suhariyadi Suhariyadi Suliati Suliati Suliati Suliati Suliati Sumasto, Hery Vemy Rahmany Kurniawan Win Darmanto Wisnu Isntanto Yusianti Silviani Yuyun Dwi Wahyuni

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