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All Journal Saintika Medika: Jurnal Ilmu Kesehatan dan Kedokteran Keluarga. Jurnal Kedokteran Brawijaya Indonesian Journal of Cardiology Jurnal Respirologi Indonesia Jurnal Multidisiplin Madani (MUDIMA)
Djoni Djunaedi
Guru Besar Ilmu Penyakit Dalam RS Universitas Muhammadiyah Malang
Civil Engineering, Building, Construction & Architecture Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Health Professions Industrial & Manufacturing Engineering Medicine & Pharmacology Public Health Social Sciences

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THE ROLE OF BIOTECHNOLOGY IN DENGUE VIRAL INFECTION : EFFORT FOR THE DEVELOPMENT OF A DENGUE VACCINE Djunaedi, Djoni
Saintika Medika: Jurnal Ilmu Kesehatan dan Kedokteran Keluarga Vol 7, No 1 (2011): Januari 2011
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (0.036 KB) | DOI: 10.22219/sm.v7i1.1080

Abstract

Introduction: Dengue fever and Dengue Haemorrhagic Fever (DF/DHF) are caused by dengue viruses (DENV). There are four antigenically related but distinct DENV serotypes (DENV-1 through DENV-4). Humans are the amplifying vertebrate host and Aedes mosquitos are the primarily mosquito vector as well as the reservoir of infection. DENV infection causes a spectrum of diseases, ranging from asymptomatic infections to infections complicated by haemorrhagic shock and death (Yoksan, 2008). Epidemiology: WHO (2008) estimates that about 2.5 billion people or 40% of the world?s population live in areas where there is a risk of dengue transmission. About 500,000 cases of dengue?s severest form (DHF/DSS) occur annually, resulting in about 24,000 deaths, mostly among children. Tropical and subtropical areas of South East Asia and Latin America are the hardest hit by dengue infection. Although dengue rarely occurs in the continental United States, it is endemic in Puerto Rico and Hawaii, a U.S. territory. Mosquitos capable of transmitting the virus have been found in the U.S. (in Florida, Georgia, Louisiana, Alabama, Mississippi, and Texas) over the last 10 years (Cano and Bannister, 2001; CDC, 2009; Science News, 2010). Facing this problem, vaccination is the answer. Vaccine Development: Dengue is an expanding public health problem, and an effective vaccine remains elusive. Significant influence of sequential infections with different dengue virus serotypes on the severity of disease can be viewed in terms of beneficial and detrimental effects of the heterologous immunity. A more complete understanding of these effects is likely to be critical for predicting optimal vaccine ? induced immune responses, with the aim of protecting the global population from emerging infectious disease threats (Rothman, 2004). In 1980, Mahidol University committed to develop a live-attenuated tetravalent DENV vaccine. They were subjected to general safety test and monkey neurovirulence tests in accordance with the U.S. FDA requirements. (Yoksan 2008) Results: All vaccine recipients developed either a mild or no adverse reaction to the vaccine, the immunogenicity data were discussed. The current strategy of creating tetravalent DENV vaccine formulation can lead to an unbalanced immune response. This is attributed to viral interference that apparently comes into play when three monovirulent vaccine viruses DENV-1, DENV-2, DENV-4 are mixed with DENV-3 to create a tetravalent formulation (Yoksan, 2008). Summary: More research is needed on a priority basis to work out the viral interference factor in order to make the production of a tetravalent vaccine out of the attenuated DENV-3 candidate vaccine strain a success. Key words: Dengue Haemorrhagic Fever, Shock and death, tetravalent dengue vaccine, viral interference factor.
PERUBAHAN KADAR SITOKIN DAN MOLEKUL AGREGASI PADA BERBAGAI TINGKAT TROMBOSITOPENIA PADA DEMAM BERDARAH DENGUE Djunaedi, Djoni
Jurnal Kedokteran Brawijaya Vol 21, No 1 (2005)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (134.882 KB) | DOI: 10.21776/ub.jkb.2005.021.01.3

Abstract

The uncovering of the changes in the concentration of aggregation molecules (vWF, PGI2) and cytokines (TNFα, IL-1β, IL-6) in different levels of thrombocytopenia in DHF patients can be used as the basis in predicting the occurrence of thrombocytopenia. A prospective-observational studywas undertaken utilizing a 3-day sequentially drawn vein blood of inclusive DHF patients. The TNFα, IL-1β and IL-6 test was conducted utilizing the quantikine high sensitivity human cytokine immunoassay, and the vWF and PGI2test was conducted utilizing the ELISA procedure.  The multivariate and discriminant analyses tests on the deltas between day 1 and 2 observations, and between day 2 and 3 observations of the 6 variables showed Wilks’ Lambda =0.638 with p=0.008 and platelets and vWF as the most powerful discriminating variable (70.5.%). The findings of this study indicated that in DHF patients (1) the changing of the concentration of TNFα, IL-1βand IL-6 cytokines do not play a significant role  in the mechanism of platelets aggregation, (2) platelets count decreases in a condition in which vWF facilitated platelets aggregation, (3) the changing of a positive contribution of vWF causes a determination of the process of platelets aggregation, and (4) a positive changing of vWF’s concentration can be used as a prognostic indicator of a negative changing of platelets count.. Key words: Dengue Hemorrhogic Fever, thrombocytopenia, vWF
PENGARUH PROBIOTIK PADA RESPON IMUN Djunaedi, Djoni
Jurnal Kedokteran Brawijaya Vol 23, No 1 (2007)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (126.214 KB) | DOI: 10.21776/ub.jkb.2007.023.01.4

Abstract

Probiotics (“for life”) is a live microbial feed supplement or defined as mono- or mixed cultures of live microorganisms which when administered in adequate amounts confer  a health benefit to the host by improving its microbial balance. Common descriptives for probiotics include “friendly”, “beneficial” or “healthy” bacteria.  Under natural conditions a protective gut microflora develops and there is no  need for probiotics supplement, but under rather un-natural conditions such like intestinal infections, post antibiotic treatment syndromes, pseudomembranous colitis, the probiotics preparations are needed. The beneficial effects of probiotics inthe host are promotion of the gut maturation and integrity, antagonisms against pathogens and immune modulation. Beyond those, the microflora seems to play a significant rolein diet induced superinflammation, mucosal immune system, neuroendocrine regulation, immunoglobulin production, macrophages factor restoration, apoptosis stimulation, lymphocyte function modulation, cytokine release, mucin production, intestinal immune homeostasis and inflammation prevention. However, many studies proved that probiotics have “upregulation and down-regulation” effects on immune system of the host.
JENIS BAKTERI DAN SENSITIVITAS ANTIBIOTIK PADA KASUS INFEKSI NOSOKOMIAL AKIBAT PEMASANGAN KATETER DI RSSA MALANGDALAM PERIODE NOVEMBER 2000 - MARET 2001 Djunaedi, Djoni
Jurnal Kedokteran Brawijaya Vol 22, No 3 (2006)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (109.792 KB) | DOI: 10.21776/ub.jkb.2006.022.03.2

Abstract

The incidence of Nosocomial Infection (NI) includinginfection of the urinary tract attributable to catheter insertion aggravates the existing conditions. Local symptoms  and septis that occured could prolong hopitalization, and even caused death. So far, prevention of NI has been undertaken only through the adherence to the standard  procedure. The important problem that arises is whether the prevention of NI should rely only on the adherence of thestandard procedure. In order to find the solution, an appropriate investigation is called for. The materials utilized were (a) urine collected from obstetric-gynaecological patients that required catheter insertion – urine sample was taken using antiseptical procedures before and after catheter insertion within the period of 2x24 hours, (b) the end section of catheter canule when the the catheter was taken off. The materials were cultured in blood plates. Bacterial counts were performed on the data with respect to bacterial types and their sensitivity to antibiotics of the families of Cephalosporin (Cefotaxim), Aminoglycoside (Amikacin), Quinolone (Ciprofoxacine), Penicilline (Amoxicilline, Ampicilline), cotrimoxazole and Nitrofurantoin.
THE ROLE OF BIOTECHNOLOGY IN DENGUE VIRAL INFECTION : Effort for The Development of A Dengue Vaccine Djoni Djunaedi
Saintika Medika Vol. 7 No. 1 (2011): Januari 2011
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/sm.v7i1.1080

Abstract

Introduction: Dengue fever and Dengue Haemorrhagic Fever (DF/DHF) are caused by dengue viruses (DENV). There are four antigenically related but distinct DENV serotypes (DENV-1 through DENV-4). Humans are the amplifying vertebrate host and Aedes mosquitos are the primarily mosquito vector as well as the reservoir of infection. DENV infection causes a spectrum of diseases, ranging from asymptomatic infections to infections complicated by haemorrhagic shock and death (Yoksan, 2008). Epidemiology: WHO (2008) estimates that about 2.5 billion people or 40% of the world’s population live in areas where there is a risk of dengue transmission. About 500,000 cases of dengue’s severest form (DHF/DSS) occur annually, resulting in about 24,000 deaths, mostly among children. Tropical and subtropical areas of South East Asia and Latin America are the hardest hit by dengue infection. Although dengue rarely occurs in the continental United States, it is endemic in Puerto Rico and Hawaii, a U.S. territory. Mosquitos capable of transmitting the virus have been found in the U.S. (in Florida, Georgia, Louisiana, Alabama, Mississippi, and Texas) over the last 10 years (Cano and Bannister, 2001; CDC, 2009; Science News, 2010). Facing this problem, vaccination is the answer. Vaccine Development: Dengue is an expanding public health problem, and an effective vaccine remains elusive. Significant influence of sequential infections with different dengue virus serotypes on the severity of disease can be viewed in terms of beneficial and detrimental effects of the heterologous immunity. A more complete understanding of these effects is likely to be critical for predicting optimal vaccine – induced immune responses, with the aim of protecting the global population from emerging infectious disease threats (Rothman, 2004). In 1980, Mahidol University committed to develop a live-attenuated tetravalent DENV vaccine. They were subjected to general safety test and monkey neurovirulence tests in accordance with the U.S. FDA requirements. (Yoksan 2008) Results: All vaccine recipients developed either a mild or no adverse reaction to the vaccine, the immunogenicity data were discussed. The current strategy of creating tetravalent DENV vaccine formulation can lead to an unbalanced immune response. This is attributed to viral interference that apparently comes into play when three monovirulent vaccine viruses DENV-1, DENV-2, DENV-4 are mixed with DENV-3 to create a tetravalent formulation (Yoksan, 2008). Summary: More research is needed on a priority basis to work out the viral interference factor in order to make the production of a tetravalent vaccine out of the attenuated DENV-3 candidate vaccine strain a success. Key words: Dengue Haemorrhagic Fever, Shock and death, tetravalent dengue vaccine, viral interference factor.
Efek Ekstrak Kulit Manggis (Garcinia Mangostana Linn) sebagai Anti Inflamasi pada Tikus Model Aterosklerotik Salva Reverentia Yurista; Fathiyah Safithri; Djoni Djunaedi; Djanggan Sargowo
Jurnal Kardiologi Indonesia Vol. 33, No. 1 Januari - Maret 2012
Publisher : The Indonesian Heart Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30701/ijc.v33i5.68

Abstract

Background. Atherosclerosis is an inflammatory disease characterized by intense immunological activity, which immune responses participate in every phase of atherosclerosis. On fundamental importance in atherogenesis is nuclear factor-?B(NF- ?B) that regulates activation of adhesion molecules (ICAM-1) and secretion of pro-inflammatory cytokines such as tumor necrosis factor(TNF-a) interleukin-1(IL-1), and interleukin-6(IL-6).Aims. The extract from pericarp of mangosteen (Garcinia mangostana Linn) were carried out to investigate for anti-inflammatory activity in rat models with atherosclerosis.Methods. The subjects of the study were 30 albino rats of (Rattus novergicus Strain wistar), divided into 5 groups with six rats in each group. Group I comprises normal healthy rats remains untreated for 90 days; Group II comprises of high cholesterol diet induced atherosclerotic rats for 90 days; Group III, IV, and V comprise of high cholesterol diet induced atherosclerosis and were administered extract from pericarp of mangosteen orally (200mg/kg; 400 mg/kg, 800 mg/kg) daily for 90 days. After 90 days treatment, rats were decapitated and blood was sample collected from the aorta for determination level of TNF-aand IL-1 by ELISA. Aorta was taken to observe the expression of NF-kB, ICAM-1, and IL-6 using immunohistochemical techniques.Results. Oral administration (800 mg/kg) of the extract from pericarp of mangosteen significantly decreased the level of TNF-a, and IL-1 and also reduced the expression of NF-kB, ICAM-1, and IL-6.Conclusion. These results suggest that the extract from pericarp of mangosteen (Garcinia mangostana Linn)possess anti-inflammatory actions by reducing level of TNF-a, IL-1, and also reduce the expression of NF-kB, ICAM-1, and IL-6 that prevent the development of atherosclerosis.
Systematic Review: Potential of Garlic Extract (Allium Sativum) as a Treatment for Diarrhea (E. Coli) R Mohamad Javier; Laksmitha Saktiono Safitri; Lillya Yasmine Nur Baiti; Prima Sultan Hudiyanto; Irma Suswati; Hawin Nurdiana; Djoni Djunaedi
Jurnal Multidisiplin Madani Vol. 3 No. 1 (2023): January, 2023
Publisher : PT FORMOSA CENDEKIA GLOBAL

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55927/mudima.v3i1.2500

Abstract

Diarrheal disease continues to be one of the major public health problems worldwide, with more than 1.5 million deaths each year and most of them in children under 5 years of age. The incidence of diarrheal diseases averages 2-3 episodes per child per year. Excessive use of antibiotics as antidiarrheals is associated with an increase in bacterial resistance to antibiotics, medical costs, and the incidence of side effects. In past research, it has been found that garlic has a wide antimicrobial spectrum so that it can kill gram-negative and gram-positive bacteria, so it can be used as an alternative treatment for diarrhea. The purpose of carrying out this study was to describe the potential for garlic extract that have an effect on gram-negative bacteria that cause diarrhea, this natural ingredient can be used as a safe alternative treatment to treat infectious diseases that cause gram-negative bacterial diarrhea. This research method is Narrative review (Literature review). In a journal that discusses the effectiveness of garlic as an antimicrobial regarding the antibacterial activity of thiosulfinates using the petri plate diffusion test, the efficacy of the substances cannot be compared with each other because the assumed diffusion rates are different. In the journal on Comparison of the antibacterial activity of thiosulfinates using the petri plate diffusion test, the efficacy of the substances cannot be compared with each other because the assumed diffusion rates are different. The results showed that the MIC and MBC results of each bacteria given garlic extract were different, even though they were both gram-negative bacteria. Garlic extract has the highest effectiveness on E. coli bacteria based on in vitro studies conducted, compared to Salmonella, Shigella, V. cholerae bacteria according to the characteristics of these bacteria
Anatomical Pathology Differences in Lung Alveoli Damage with Exposure to Conventional and Electric Cigarettes Citra Paramita Esti Cahyaningrum; Desy Andari; Djoni Djunaedi
Jurnal Respirologi Indonesia Vol 43, No 2 (2023)
Publisher : Perhimpunan Dokter Paru Indonesia (PDPI)/The Indonesian Society of Respirology (ISR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36497/jri.v43i2.191

Abstract

In conventional cigarettes, tobacco is a major risk factor in the development of diseases involving the lungs, including pulmonary emphysema, fibrosis and lung cancer. Many people think that using e-cigarettes is much safer than conventional cigarettes. Whereas smoking with electronic cigarettes can cause the same feeling of cotton mouth as felt by conventional smokers, with symptoms such as itchy throat, cough and complications to the lungs. This literature review conducted a literature search with the keywords cigarette, e-cigarette, popcorn lung, and alveoli. Conventional cigarettes and electronic cigarettes (e-cigarettes) cause damage to the pulmonary alveoli in the form of alveolar spaces; this depends on the nicotine content in them. Electronic cigarettes and conventional cigarettes exert different effects on the oxidative stress response of the airway epithelium. In addition, the image of popcorn lung can be found due to the presence of diacetyl that appears when heating e-juices in e-cigarettes.
The Relationship Between Neutrophil-Lymphocyte Ratio (NLR), C-Reactive Protein (CRP), Interleukin 6 (IL-6), and D-Dimer Levels on the Severity of Covid-19 Patients at RSU Muhammadiyah University of Malang Hermayanti, Diah; Thahri Iskandar; Djoni Djunaedi; Silvana Gadis Ardhistix’s; Maulana Faishol Charisma M; Fajrul Falah; Muhammad Samir
Saintika Medika Vol. 19 No. 2 (2023): December 2023
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/sm.Vol19.SMUMM2.33202

Abstract

Background: COVID-19 virus infection has the potential to become severe in individuals who are susceptible or who have comorbid diseases. Natural immunity status in infected sufferers can cause an increase in the number of neutrophil cells and a decrease in the number of lymphocyte cells (increased neutrophil-lymphocyte ratio), serum inter-leukin-6 (IL-6), C-reactive protein (CRP) levels which are associated with severity. covid-19. Hypercoagulability that occurs in COVID-19 infection causes an increase in D-dimer levels, reflecting a response to prothrombotic phenomena and is associated with an increased risk of mortality in COVID-19 patients. Objective: To determine the relationship between Neutrophil-lymphocyte ratio (NLR), C-reactive protein (CRP), Interleukin-6 (IL-6), and D-dimer levels on the severity of Covid-19 patients at Muhammadiyah Malang University (UMM) Hospital Method: Using a retrospective design by taking a total sampling of medical record data for COVID-19 patients treated at UMM Hospital in 2021-2022. Results and Discussion: Spearman correlation on NLR 0.426 (p=0.000), OR=3.67, 95% CI=1.622-8.226, CRP 0.343 (p=0.000) with OR 5.143, 95%CI=1.128-23.448, D- Dimer 0.326 (p=0.000), OR 2.497, 95% CI=1.142-5.458. Meanwhile, the correlation and IL-6 estimation values could not be calculated because the sample size was insufficient. CRP levels in COVID-19 patients are the best predictor of worsening compared to other parameters. Conclusion: NLR values, CRP levels, and D-Dimer levels are related to the degree of severity in COVID-19 patients.
Co-Authors Citra Paramita Esti Cahyaningrum Desy Andari Diah Hermayanti Djanggan Sargowo Fajrul Falah Fathiyah Safithri Hawin Nurdiana Irma Suswati Laksmitha Saktiono Safitri Lillya Yasmine Nur Baiti Maulana Faishol Charisma M Muhammad Samir Prima Sultan Hudiyanto R Mohamad Javier Salva Reverentia Yurista Silvana Gadis Ardhistix’s Thahri Iskandar