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All Journal BIOLINK (Jurnal Biologi Lingkungan, Industri, Kesehatan) Agrotekma: Jurnal Agroteknologi dan Ilmu Pertanian Pelita Masyarakat
Muhammad Usman
Universitas Medan Area
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Environmental Science Physics Public Health Social Sciences

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UJI AKTIVITAS DAN IDENTIFIKASI JAMUR RHIZOSFER PADA TANAH PERAKARAN TANAMAN PISANG (Musa paradisiaca) TERHADAP JAMUR FUSARIUM Dewi Nur Anggraeni; Muhammad Usman
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol 1, No 2 (2015): Februari
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v1i2.729

Abstract

The main cause of decline in banana production is fusarium wilt caused by the fungus Fusarium oxysporum f.sp.cubense (Foc). This disease is systemic, it is very dangerous because it can kill plants. Fusarium wilt disease ranks first in the list of diseases of bananas in Indonesia, fusarium wilt very difficult to control because it has not found a commercial banana cultivars resistant. Integrated Pest Management (Biological Control) using biological agents, one of which is the rhizosphere that has the ability to suppress the growth of Fusarium wilt (Fusarium oxysporumf.sp.cubense) in vitro. The purpose of this study was to determine the ability of an antagonist of the rhizosphere fungus to fungus fusarium. This research method is a rhizosphere fungal activity test against fusarium fungus. The results showed that 12 isolates the rhizosphere has the potential to inhibit the growth of fungi fusarium the percentage inhibition ie 82% of isolates A1, B2 D3 by 83% and 80%, A2 isolates the isolates that produce inhibition of the smallest amounting to 15%. Based on identification consists of rhizosphere fungi genus Trichoderma sp., Aspergillus sp., And Penicillium sp.
UJI ANTI MIKROBA EKSTRAK AKAR SOM JAWA (Tanilum paniculatum, Jacq. (Gaertn)) Sartini Sartini; Muhammad Usman
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol 1, No 1 (2014): Agustus
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v1i1.15

Abstract

Antimicrobial test was conducted on plant root extract som jawa (Talinum paniculatum, jacq. (Gaertn)). Five hundred gram simplicia of som jawa root was macerated using methanol and n-heksana, and coagulated. The screening of extract methanol and n-heksana was done to get metabolic secunder; flavonoid, alkaloid, terpenoid, and saponin. The result of phytochemistry secreening test to extract methanol to metabolic secunder was negative. Antimicrobial test result showed that the extract of som jawa root  is active againts microbes E.coli. Measurement is based on the test zone diameter of the clear zone generated, where . coli has the highest diameter of the clear zone compared with the others microbes such as Staphylococus aureus, Bacillus sp, Serratia sp, and Candida albicans. The values of the clear zone of each microbe  in order are 1,1 cm, 1 cm, 0,95cm, and 0,9 cm. These value were obtain at a concentration of 10%.
PEMANFAATAN KITOSAN DARI LIMBAH CANGKANG KERANG BULU(Anadara inflata) SEBAGAI BAHAN PENJERNIH AIR SUNGAING DARI LIMBAH KULIT DURIAN Rosliana Lubis; Muhammad Usman
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol 1, No 1 (2014): Agustus
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v1i1.16

Abstract

The title of  this study is the utilization of chitosan from shellfish waste as water purifier. The purpose of the study is to utilize coagulant of shellfish waste in water treatment proes into clear water. The research variabel are the concentration of chitosan 1,3,5,7, and 9 g/l. The particle size of chitosan used in this research is 150 mesh. The time consume  8 hours for purification process. The result showed that 9 g/l chitosan shell waste is effective to water purification process. At a concentration of 9 g/l. Chitosan shellfish waste can produce clean water in complianee with health minister No. 416/MENKES/PER/IX/1990 about  water quality treatment.
PKM Pelatihan Dan Penerapan Budidaya Tanaman Secara Hidroponik Pada Kelompok PKK (KWT) Desa Sei Rotan Kecamatan Percut Sei Tuan Kabupaten Deli Serdang Asmah Indrawati; Rizal Aziz; Mitra Musika Lubis; Muhammad Usman
Pelita Masyarakat Vol. 4 No. 1 (2022): Pelita Masyarakat, September
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/pelitamasyarakat.v4i1.6886

Abstract

Efforts to increase the income of the PKK KWT group in Sei Rotan village are carried out by the Tridarma activity of higher education. The objectives of the service activities include increasing efforts to maximize house yard land management efforts by cultivating horticultural plants in the yard, and increasing the knowledge, understanding and skills of the community on hydrophonic plant cultivation techniques in the PKK KWT group community, Sei Rattan Village, Percut Sei Tuan District, and to improve community income which is classified as fitting to meet daily life, the implementation method applied is by conducting training and mentoring, in the training activities training is carried out: Hydrophonic Cultivation Training vertically and horizontally, Technical culture training for HEALTHY Horticulture plants through hydrophonic seeds, while in Mentoring: Guidance and Community Development who have started to apply technology, Discussion and Evaluation. the results of the activities based on the transfer of knowledge obtained a statement 60% of the community's responses strongly agree, 30% agree, increasing community knowledge after training obtained very good data 25%, good 60% and good enough 9%, and not good 0% and responses Satisfaction with PKM activities shows the data is very useful 42% and useful 52% and quite useful 2% and 0% which states not useful. The three categories of community responses show that the activities that have been carried out show that it is very important for the community to consider community knowledge and skills that are very useful for improving the quality of these farmer groups.
ANALYSIS OF FORMULATION MEDIA FOR PLANTING OYSTER MUSHROOM (Pleurotus ostreatus)AGRICULTURAL WASTE BASED MATERIALS Siti Mardiana; Ellen Lumisar Panggabean; Indah Apriliya; Muhammad Usman
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol. 9 No. 2 (2023): February
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v9i2.7504

Abstract

The effort of Indonesian people to fulfill their protein intake in an economically accessible way is by producing and consuming oyster mushrooms, because their protein content was viable as an alternative to animal-based products. Combination of sugarcane bagasse, palm fronds (PKS), and sawdust can be used as growing medium for oyster mushrooms. The aim of this study was: (1) to analyze the change in the pH of the combination media formulation of Palm Oil Frond Waste (PKS), sugarcane bagasse, and sawdust; (2) identifying the composition of nutrients in the aforementioned agricultural waste as a growing medium for oyster mushroom. This study was composed of two steps: 1) Preparing the composition of the growing medium from agricultural waste; and 2) Analyzing nutrient composition of the growing medium of oyster mushroom. The research was conducted by using Analysis of Variance (ANOVA) with a Non-Factorial Completely Randomized Design (RAL). The combination of oyster mushroom growing medium from palm fronds, sugarcane bagasse, and sawdust has different pH levels and nutritional contents. The combination of M0 – M8 oyster mushroom growing medium has the suitable level of pH to grow the mushrooms at 6,5 – 8,0 pH. From the formulation analysis of nutrient in the combination of M0 – M8, it can be used as a reference for the nutrient content of oyster mushrooms growing medium from palm fronds and sugarcane bagasse.
Peran Bioteknologi Mikroba Dalam Pengembangan Biofestisida Ramah Lingkungan Untuk Mendukung Pertanian Berkelanjutan Anggun Sianipar; Saipul Sihotang; Ivan Permana Putra; Juni Kasih Lombu; Shara Nafisah; Gracia Christy Sitompul; Zulheri Noer; Cut Nelly; Rita Fitriani; Muhammad Usman
Agrotekma: Jurnal Agroteknologi dan Ilmu Pertanian Vol. 10 No. 2 (2026): Agrotekma: Jurnal Agroteknologi dan Ilmu Pertanian, 10 (2) Juni 2026
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/agr.v10i2.18319

Abstract

Abstrak Penggunaan pestisida sintetis secara intensif masih menjadi salah satu strategi utama dalam pengendalian organisme pengganggu tanaman. Namun, penggunaan jangka panjang dapat menimbulkan dampak negatif terhadap lingkungan, kesehatan manusia, resistensi hama, serta penurunan keanekaragaman hayati. Bioteknologi mikroba menawarkan pendekatan alternatif melalui pemanfaatan mikroorganisme potensial sebagai agen biopestisida ramah lingkungan. Artikel review ini bertujuan untuk membahas peran bioteknologi mikroba dalam pengembangan biopestisida berbasis bakteri, cendawan, virus, dan aktinomiset, serta kontribusinya terhadap sistem pertanian berkelanjutan. Metode yang digunakan adalah kajian literatur terhadap artikel ilmiah, regulasi, dan laporan terkait biopestisida mikroba, mekanisme kerja, formulasi, aplikasi lapangan, serta tantangan komersialisasi. Hasil kajian menunjukkan bahwa mikroba seperti Bacillus thuringiensis, Bacillus subtilis, Pseudomonas fluorescens, Trichoderma spp., Beauveria bassiana, Metarhizium anisopliae, dan Streptomyces spp. memiliki potensi besar sebagai agen pengendali hayati melalui mekanisme antibiosis, kompetisi, mikoparasitisme, produksi enzim litik, produksi metabolit sekunder, serta induksi ketahanan sistemik tanaman. Pengembangan biopestisida mikroba masih menghadapi kendala berupa stabilitas formulasi, konsistensi efektivitas di lapangan, regulasi, umur simpan, serta penerimaan petani. Oleh karena itu, integrasi bioteknologi mikroba, formulasi modern, pendekatan omics, dan sistem pengendalian hama terpadu menjadi strategi penting dalam memperkuat pemanfaatan biopestisida untuk pertanian berkelanjutan. Kata kunci: bioteknologi mikroba; biopestisida; agen hayati; pertanian berkelanjutan; pengendalian hayati. Abstract The intensive use of synthetic pesticides remains one of the main strategies for controlling plant pests and diseases. However, long-term application may cause environmental degradation, pest resistance, biodiversity loss, and potential risks to human health. Microbial biotechnology offers an alternative approach through the utilization of beneficial microorganisms as environmentally friendly biopesticides. This review aims to discuss the role of microbial biotechnology in the development of biopesticides based on bacteria, fungi, viruses, and actinomycetes, as well as their contribution to sustainable agriculture. The method used in this study was a literature review of scientific articles, regulations, and reports related to microbial biopesticides, mechanisms of action, formulation, field application, and commercialization challenges. The review shows that microorganisms such as Bacillus thuringiensis, Bacillus subtilis, Pseudomonas fluorescens, Trichoderma spp., Beauveria bassiana, Metarhizium anisopliae, and Streptomyces spp. have strong potential as biological control agents through antibiosis, competition, mycoparasitism, lytic enzyme production, secondary metabolite production, and induced systemic resistance. Nevertheless, microbial biopesticide development still faces several challenges, including formulation stability, field efficacy consistency, regulation, shelf life, and farmer acceptance. Therefore, integrating microbial biotechnology, modern formulation, omics approaches, and integrated pest management is essential to strengthen the role of microbial biopesticides in sustainable agriculture.
Co-Authors Anggun Sianipar Asmah Indrawati Cut Nelly Dewi Nur Anggraeni Ellen Lumisar Panggabean Gracia Christy Sitompul Indah Apriliya Ivan Permana Putra Juni Kasih Lombu Mitra Musika Lubis Rita Fitriani Rizal Aziz Rosliana Lubis Saipul Sihotang Sartini Sartini Shara Nafisah Siti Mardiana Zulheri Noer