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All Journal Farmasains : Jurnal Farmasi dan Ilmu Kesehatan
Andi Muflihunna
Faculty of Pharmacy, Indonesian Muslim University, Makassar, South Sulawesi, Indonesia
Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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Analysis of antioxidant activity of carrots (Daucus carrota L.) using FRAP and CUPRAC methods Aprilya Sri Rachmayanti; Nurmaya Effendi; Andi Muflihunna
Farmasains : Jurnal Farmasi dan Ilmu Kesehatan Vol. 6 No. 1 (2021)
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/farmasains.v6i1.13981

Abstract

Carrot antioxidant activity analysis has been carried out. Carrot juice was analyzed qualitatively by the FRAP method using FRAP reagent and by the CUPRAC method using copper (II) chloride solution, neucuproine solution, ammonium acetate buffer solution, and distilled water. Quantitatively, the mixture of sample solutions using the FRAP and CUPRAC methods was analyzed using a UV-Vis spectrophotometer at a wavelength of 596 nm for the FRAP method and 452 nm for the CUPRAC method, using Trolox solution as the standard curve. The results obtained were that each sample A and B's antioxidant activity using the FRAP method was 0.5186 µM TR/g and 0.8857 µM TR/g samples. Meanwhile, samples A and B's antioxidant activity using the CUPRAC method was 0.1160 µM TR/g sample and 0.1762 µM TR/g sample.
Co-Authors Aprilya Sri Rachmayanti Nurmaya Effendi