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Endang Ediningsih
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Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology

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Efek Hepatoprotektif Sari Belimbing Manis Terhadap Kadar SGPT: Studi Awal Terhadap Tikus yang Diinduksi Parasetamol Dosis Toksik Amatillah, Hana; Ediningsih, Endang; Hikmayani, Nur Hafidha
Nexus Biomedika Vol 2, No 3 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

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Abstract

Background: Star fruit is a good source of natural antioxidants which can prevent oxidative damages and modulate reactive free-radicals. However, no study had been carried out so far on the hepatoprotective effect of star fruit. This research aimed to study the hepatoprotective effect of star fruit juice on SGPT level in paracetamol- intoxicated rats. Methods: This was a laboratory experimental study with posttest only controlled group design. A total of 30 white male, Wistar rats (Rattus norvegicus), aged 2-3 months and weighed 160-200 grams was assigned into five groups. Normal group and negative control group were administered distilled water, while positive control group was administered Curcuma® (3.6 mg/200 g bw). The treatment group 1 and 2 were administered star fruit juice at concentration of 50% and 100%, successively. This study was performed for 14 days. In the last 3 days, all groups except normal group were induced by paracetamol at toxic dose (291.6 mg/200 g BW) an hour after the treatment of star fruit juice (for treatment group 1 and 2) was done. Blood samples were collected from retro-orbitalis venous to measure SGPT levels. The data were analyzed statistically using one-way ANOVA followed by LSD test. Results: The analysis of SGPT with one-way ANOVA showed a significant difference across all groups. Further, the LSD tests of SGPT level showed significant differences between negative control group (182.5 U/L ± 14.0) and the two star fruit groups (p values <0.001). SGPT mean in treatment group 1 (87.0 U/L ± 14.1) and treatment group 2 (91.7 U/L ± 14.9) also differed from those of positive control (67.1 U/L ± 5.5, p=0.008) and normal group (53.8 U/L ± 8.6, p=0.002). There was no significant difference between two groups treated with star fruit juice (p=0.511). Conclusions: Star fruit juice at concentration of 50% and 100%  significantly inhibited the elevation of SGPT levels in paracetamol-intoxicated rats. Keywords: Star fruit juice, SGPT, paracetamol, rats 
Pengaruh Ekstrak Daun Kemangi terhadap Penurunan Kadar Kreatinin dalam Darah Tikus Putih yang Diinduksi Parasetamol Wibowo, Arianto Adi; Ediningsih, Endang; ., Samigun
Nexus Kedokteran Translasional Vol 1, No 1 (2012): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Abstract

Background: Induced by parasetamol in large quantities will damaging in proximal tubule. Creatinine which should secreted by glomerolus will back in blood stream if occur kidney obstruction that which decreasing in-blood levels of creatinine. The purpose of this research is to find out whether the extract of kemangi has an effect to decrease in-blood levels of creatinine in white rats induced by parasetamol. Methods. The research was an experimental laboratoric study with the post test only controlled group design. This study was done in Setia Budi University. Subjects in this research were tirthty male white rats (Rattus norvegicus), 2-3 month of age and 150-220 gram of each weight. Subjects were divided into 5 groups.Group I as a negative control only received normal saline. Group II as a positive control  and group III, IV, and V  received parasetamol (150 mg/200 g body weight) every 3 day for 10 days. Group III received extract kemangi at 40mg / 200g body weight every 3 day for 10 days. Group IV received extract kemangi at 80mg / 200g body weight every 3 day for 10 days. Group V received extract kemangi at 120 mg 200 g body weight every 3 day for 10 days. Treatment were conducted on 11st after a 10-day adaptation. Blood sampling via plexus vena orbita of white rats for creatinine levels measured were conducted on 21st day. Then data was analyzed using One-Way ANOVA and LSD. Result. Result of One-Way ANOVA showed that there was a significant different between 5 groups: positive and negative control groups, and three dosages difference treatment (40mg, 80mg, 120mg / 200gr BB). Result of LSD showed that there was a significant different between positive control group and treatment groups; and between control group,  but there was not the significant different between negative control group and treatment groups; and each treatment groups. Conclusion. All of the extract kemangi dosages has significantly effect to decrease in- blood levels of creatinine in white rats induced by parasetamol. Statisticaly, there is no significant different in the dose diffenrence at treatment 1, 2 and 3 (40mg, 80mg, 120mg / 200gr BB). Keywords: extract kemangi, decrease in-blood levels of creatinine, parasetamol.
The Effectivity of Low Dose Steroid for Reducing Intestinal Inflammation Grade in Mice Model of Sepsis Early Stage Medita Prasetyo; Endang Ediningsih; Sarsono .
Nexus Biomedika Vol 4, No 1 (2015): Nexus Biomedika
Publisher : Nexus Biomedika

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Background: Sepsis is a systemic inflammatory response syndrome, which activates apoptosis of the mucous lining of gastrointestinal tract. The use of steroids as inflammatory therapies in sepsis is still debated. This study aimed to determine the effect of steroid in low dose on intestinal inflammation grade in mice model of Sepsis early stage. Method : This study was an experimental laboratory with the post test only controlled group design. The sample was 25 male Balb/C mice, divided into five groups, namely: control (K1), sepsis (K2), sepsis + low dose steroid (K3), sepsis + moderate dose steroid (K4), sepsis + high dose steroid (K5). The models of sepsis were given cecal inoculums material 4 mg/kgBW/day/i.p for 5 days. Two hours after i.p injection of cecal inoculums, the mice were given dexamethasone with doses 0.05; 0.25; 2.50 mg/kgBW/day/i.v, representing low, moderate, and high dose steroid therapy for 5 days. Histological preparation of intestines was made on day 6, while the anti inflammatory effect of steroid therapy was measured based on inflammatory grading according to Chang et al. Data were analyzed using Kruskal Wallis method and Mann Whitney method. The values of p < 0.05 were considered statistically significant. Result : The study showed grading inflammation of mice within groups were: K1: grade 0 (100%); K2: grade 2 (60%) and grade 3 (40%); K3: grade 0 (40%) and grade 1 (60%); K4: grade 2 (20%) and grade 3 (80%); K5: grade 3 (40%) and grade 4 (60%). There was significant difference between groups: K1-K2 (p = 0.005), K2-K3 (p = 0.007), K2-K5 group (p = 0.020), K3-K4 (p = 0.006), K3-K5 (p = 0.007), K4-K5 (p = 0.042). Conclusion: Low dose steroid effectively reduces intestinal inflammation grade in mice model of sepsis early stage. Keywords: Steroid dose, inflammation grade, sepsis.
Diuresis Effect Comparison between Leaf Extract of Orthosiphon aristatus and Hydroclorotiazidto Male White Rat (Rattus novergicus) ANGGIE HERWANLISTANTO; Endang Ediningsih; Siti
Nexus Biomedika Vol 6, No 1 (2017): Nexus Biomedika
Publisher : Nexus Biomedika

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Abstract

Introduction: Kumis kucing leaf containing flavonol which can generate diuresis effect with increasing the excretion of natrium and chlorine. The purpose of this research was to know how did the diuresis effect of kumis kucing leaf extrack compared to hydroclorotiazid. Methods: The research was a laboratory experimental analytic study with posttest only control group design. The research subject was a number of 30 male white rats of wistar strain, aged about 2-3 months old and 150-200 grams of weight, which divided into five treatment groups. Group 1was given 2 ml CMC 1% as negative control, group 2 was given 0.32 mg hydroclorotiazid in 2 ml aquadest as positive control, group 3 was given kumis kucing leaf extract dose I (50 mg in 2 ml CMC 1%), group 4 was given kumis kucing leaf extract dose II (100 mg in 2 ml CMC 1%), group 5 was given kumis kucing leaf extract dose III (200 mg in 2 ml CMC 1%). Rats urine volume was measured every 6 hours for 24 hours observation. Result:The average measurements of rat urine volume for 24 hours showedthat group negative control had average volume3.3871.435 ml, the average volume of positive control was7.3070.607 ml,the average volume group dose I was 2.9000.350 ml, the averagevolume group dose II was 3.4900.311 mlanddose III had average volume 5.4330.641 ml. Result of anova statistical calculation showed there are significant difference urine volume in those five groups (p <0.05). Conclution:The research conclude that kumis kucing leaf extract had diuresis effect lower than hydroclorotiazid therapeutical dose. Keyword: kumis kucing leaf extract, hydroclorotiazid, diuresis
Pengaruh Ekstrak Daun Kemangi terhadap Penurunan Kadar Kreatinin dalam Darah Tikus Putih yang Diinduksi Parasetamol Arianto Adi Wibowo; Endang Ediningsih; Samigun .
Nexus Kedokteran Translasional Vol 1, No 1 (2012): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (14.205 KB)

Abstract

Background: Induced by parasetamol in large quantities will damaging in proximal tubule. Creatinine which should secreted by glomerolus will back in blood stream if occur kidney obstruction that which decreasing in-blood levels of creatinine. The purpose of this research is to find out whether the extract of kemangi has an effect to decrease in-blood levels of creatinine in white rats induced by parasetamol. Methods. The research was an experimental laboratoric study with the post test only controlled group design. This study was done in Setia Budi University. Subjects in this research were tirthty male white rats (Rattus norvegicus), 2-3 month of age and 150-220 gram of each weight. Subjects were divided into 5 groups.Group I as a negative control only received normal saline. Group II as a positive control and group III, IV, and V received parasetamol (150 mg/200 g body weight) every 3 day for 10 days. Group III received extract kemangi at 40mg / 200g body weight every 3 day for 10 days. Group IV received extract kemangi at 80mg / 200g body weight every 3 day for 10 days. Group V received extract kemangi at 120 mg 200 g body weight every 3 day for 10 days. Treatment were conducted on 11st after a 10-day adaptation. Blood sampling via plexus vena orbita of white rats for creatinine levels measured were conducted on 21st day. Then data was analyzed using One-Way ANOVA and LSD. Result. Result of One-Way ANOVA showed that there was a significant different between 5 groups: positive and negative control groups, and three dosages difference treatment (40mg, 80mg, 120mg / 200gr BB). Result of LSD showed that there was a significant different between positive control group and treatment groups; and between control group, but there was not the significant different between negative control group and treatment groups; and each treatment groups. Conclusion. All of the extract kemangi dosages has significantly effect to decrease in- blood levels of creatinine in white rats induced by parasetamol. Statisticaly, there is no significant different in the dose diffenrence at treatment 1, 2 and 3 (40mg, 80mg, 120mg / 200gr BB). Keywords: extract kemangi, decrease in-blood levels of creatinine, parasetamol.
Efek Hepatoprotektif Sari Belimbing Manis Terhadap Kadar SGPT: Studi Awal Terhadap Tikus yang Diinduksi Parasetamol Dosis Toksik Hana Amatillah; Endang Ediningsih; Nur Hafidha Hikmayani
Nexus Biomedika Vol 2, No 3 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Background: Star fruit is a good source of natural antioxidants which can prevent oxidative damages and modulate reactive free-radicals. However, no study had been carried out so far on the hepatoprotective effect of star fruit. This research aimed to study the hepatoprotective effect of star fruit juice on SGPT level in paracetamol- intoxicated rats. Methods: This was a laboratory experimental study with posttest only controlled group design. A total of 30 white male, Wistar rats (Rattus norvegicus), aged 2-3 months and weighed 160-200 grams was assigned into five groups. Normal group and negative control group were administered distilled water, while positive control group was administered Curcuma (3.6 mg/200 g bw). The treatment group 1 and 2 were administered star fruit juice at concentration of 50% and 100%, successively. This study was performed for 14 days. In the last 3 days, all groups except normal group were induced by paracetamol at toxic dose (291.6 mg/200 g BW) an hour after the treatment of star fruit juice (for treatment group 1 and 2) was done. Blood samples were collected from retro-orbitalis venous to measure SGPT levels. The data were analyzed statistically using one-way ANOVA followed by LSD test. Results: The analysis of SGPT with one-way ANOVA showed a significant difference across all groups. Further, the LSD tests of SGPT level showed significant differences between negative control group (182.5 U/L 14.0) and the two star fruit groups (p values <0.001). SGPT mean in treatment group 1 (87.0 U/L 14.1) and treatment group 2 (91.7 U/L 14.9) also differed from those of positive control (67.1 U/L 5.5, p=0.008) and normal group (53.8 U/L 8.6, p=0.002). There was no significant difference between two groups treated with star fruit juice (p=0.511). Conclusions: Star fruit juice at concentration of 50% and 100% significantly inhibited the elevation of SGPT levels in paracetamol-intoxicated rats. Keywords: Star fruit juice, SGPT, paracetamol, rats
Effect of Kersen Leaf (Muntingia calabura) Ethyl Acetate Extract on Blood Uric Acid Levels Raynalda Chriesmart Dezmonda; Siti Ma&#039;rufah; Endang Ediningsih
Nexus Biomedika Vol 6, No 1 (2017): Nexus Biomedika
Publisher : Nexus Biomedika

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Abstract

Introduction: Kersen leaves are known to contain various kinds of active substances one of which is a flavonoid, a type of flavonoid that is quersetin have a function as lowering uric acid levels through inhibition of the enzyme xanthine oxidase. This study aims to find out Kersen leaves (Muntingia calabura) Ethyl Acetate Extract Effect on Blood Uric Acid Levels in Mice (Mus muculus) Male with Hyperuricaemia Model. Methods: This study was an experimental laboratory with post test only control group design. A sample of male mice, aged 2-3 months, body weight 25-40 grams. The subjects were male mice 24 individuals, were divided into six groups randomly. Group I as normal control, group II as negative control received caffeine 0.42 mg and 0.5ml chicken liver juice orally. Group III, IV, V and VI received respectively allopurinol treatment 0.2 mg (positive control), kersen leaf extract consecutive doses 12.74 mg, 25.48 mg and 50.96 mg it orally, and induced caffeine 0.42 mg and chicken liver juice 0.5ml orally 1 hour after treatment. Measurement of uric acid levels using a GCU test strip, blood was taken from the mice tail one hour after the induction of hyperuricemia. Data were analyzed using one-way ANOVA followed post hoc LSD test Results: The result showed blood uric acid levels throughout the test animals was significantly different (p?0.05). The test results, post hoc LSD test showed that the positive control group and group IV, V and VI significantly different from the negative groups (p?0.05) while there was a significant difference in the group IV, V and VI (p ? 0.05) with the normal control group and positive control group. Conclusion: All doses can lower uric acid levels of mice significantly. And all three doses did not differ significantly of uric acid levels compare to therapeutic doses of allopurinol. Keywords: Kersen leaves, uric acid, allopurinol, caffeine, quercetin
Co-Authors ANGGIE HERWANLISTANTO Arianto Adi Wibowo Hana Amatillah Medita Prasetyo Nur Hafidha Hikmayani Nur Hafidha Hikmayani Raynalda Chriesmart Dezmonda Samigun . Sarsono . Siti Siti Ma&#039;rufah