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All Journal The Journal of Experimental Life Sciences (JELS) Research Journal of Life Science JFMR (Journal of Fisheries and Marine Research) Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Biologi Tropis
Sri Andayani
Brawijaya University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Engineering Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Physics

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PENGARUH PEMBERIAN EKSTRAK KASAR DAUN BELIMBING WULUH (Averrhoa bilmbi L.) TERHADAP DAYA HAMBAT BAKTERI Pseudomonas fluorescens SECARA IN VITRO Etika Dwi Rahmawati; Sri Andayani; Heny Suprastyani
JFMR (Journal of Fisheries and Marine Research) Vol 3, No 3 (2019): JFMR VOL 3 No. 3
Publisher : JFMR (Journal of Fisheries and Marine Research)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jfmr.2019.003.03.3

Abstract

Intensifikasi budidaya perikanan dapat menyebabkan menurunnya kondisi lingkungan yang menyebabkan penyakit pada ikan. Penyakit yang sering menginfeksi ikan adalah bakteri Pseudomonas fluorescens. Pengobatan yang sering dilakukan yaitu penggunaan antibiotik. Penggunaan antibiotik yang berlebihan dapat menyebabkan resistensi bakteri. Pengobatan alternatif digunakan pada ikan yang terserang penyakit oleh bakteri adalah dengan menggunakan bahan – bahan alami yang mengandung zat anti bakteri yang lebih ramah lingkungan dan tidak menimbulkan resistensi terhadap bakteri. Salah satunya adalah daun belimbing wuluh (A. bilimbi L.). Berdasarkan masalah tersebut maka perlu dilakukan penelitian terhadap pengaruh ekstrak kasar daun belimbing wuluh (Averrhoa bilimbi L.) terhadap bakteri P. fluorescens. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh ekstrak kasar daun belimbing wuluh (Averrhoa bilimbi L.) terhadap bakteri P. fluorescens. Metode yang digunakan pada penelitian ini yaitu metode eksperimen dengan Rancangan Acak Lengkap (RAL) dan menggunakan Uji MIC (Minimum Inhibition Concentration) dan Uji Cakram. Pada penelitian ini terdiri dari 5 perlakuan, 2 kontrol dan 3 kali ulangan. Perlakuan yang digunakan adalah ekstrak kasar daun belimbing wuluh (Averrhoa bilimbi L.) dengan dosis 5 ppm, 25 ppm, 45 ppm, 65 ppm dan 85 ppm. Hasil penelitian menunjukkan pemberian ekstrak kasar daun belimbing wuluh (Averrhoa bilimbi L.) dapat menghambat pertumbuhan bakteri dan dosis dengan hasil tertinggi terdapat pada perlakuan E dengan dosis 85 ppm.
Uji Daya Hambat Ekstrak Kasar Daun Johar (Cassia siamea L.) Terhadap Bakteri Pseudomonas aeruginosa Mira Leyana; Heny Suprastyani; Sri Andayani
JFMR (Journal of Fisheries and Marine Research) Vol 5, No 1 (2021): JFMR VOL 5 NO.1
Publisher : JFMR (Journal of Fisheries and Marine Research)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jfmr.2021.005.01.2

Abstract

Dampak penggunaan antibiotik dapat menyebabkan resistensi terhadap patogen. Alternatif digunakan untuk mengatasi masalah tersebut dengan mengubah pengobatan bahan alami yang mengandung senyawa aktif antibakteri yang lebih ramah lingkungan. Salah satu bahan alami adalah daun johar (Cassia siamea L.). Penelitian ini bertujuan untuk mengetahui pengaruh pemberian ekstrak kasar daun johar yang dapat menghambat pertumbuhan Pseudomonas aeruginosa. Metode yang digunakan adalah metode eksperimental dengan Rancangan Acak Lengkap (RAL), menggunakan Uji MIC (Minimum Inhibition Concentration) dan Uji Disk. Penelitian ini terdiri dari 5 perlakuan, 2 kontrol dan 3 ulangan. Perlakuan menggunakan ekstrak kasar daun johar (C.siamea L.) dengan dosis 110 ppm, 135 ppm, 160 ppm, 185 ppm dan 210 ppm. Hasil penambahan dosis ekstrak kasar (C. siamea L.) terhadap zona hambat menunjukkan pola linier dengan persamaan y = 8,1725 + 0,0093x dan R2 = 0,935. Hasil penelitian menunjukkan ekstrak kasar daun johar (C. siamea L.) dapat menghambat pertumbuhan P. aeruginosa, bakteri menunjukkan bahwa diameter rata-rata zona bening meningkat sejalan dengan penambahan ekstrak.
Accumulation Time and Antioxidant Responses of Inorganic Arsenic on Brown Seaweed Padina minor Wiga Alif Violando; Sri Andayani; Saou-Lien Wong; Chih-Hsuan Tseng
Research Journal of Life Science Vol 4, No 2 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (720.843 KB) | DOI: 10.21776/ub.rjls.2017.004.02.4

Abstract

Padina minor had a good ability to bind and accumulate arsenic (As). This research aimed to observe the As accumulation and detoxification time, also the antioxidant defense activities. Each gram of P. minor was culture in various As contaminate media for 7 days, then reculture in As-free media for 14 days. There was an acute effect on thali by decreasing its growth slowly till final day with small amount accumulation at concentration 25 µg iAs g-1. Meanwhile, direct attenuation impact was due at 50 and 100 µg iAs g-1 with massive accumulation. The thali starts to recover after reculture in health environment for 14 days. Only As5+ was detected on the thali at day 7 and 21 were indicates internal oxidation of As3+ was done before 7 days. Various antioxidant activities such as decreasing polysaccharide and increasing activities of DPPH and flavonoid at high level were observed. Those indicated that 50 µg iAs g-1 was optimum iAs level on P. minor. The results indicate that P. minor has ability to oxidase and methylate with antioxidant role as defensive activities against iAs, but it also needs more than 14 days to recover in high level contamination.
The Potential and Development of Seaweed Cultivation in Lombok: A Review Nunik Cokrowati; Yenny Risjani; Sri Andayani; Muhamad Firdaus
Jurnal Biologi Tropis Vol. 23 No. 1 (2023): January - March
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i1.4654

Abstract

The potential area of Lombok seaweed cultivation is 5490 Ha. The area utilized is 2039.63 Ha. The purpose of this paper is to review the diversity, cultivation activities, production and potential of seaweed to be developed, specifically focusing on aspects of cultivation and production on the island of Lombok, Indonesia. Macroalgae that have been cultivated on Lombok are Kappaphycus alvarezii morphotype brown and green and Gracilaria verucosa. The decline and the amount of uncertainty in production were caused by several problems in seaweed cultivation, weather changes, limited capital for production and decreased quality of seaweed seeds. The species that have the potential to be developed are Sargassum aquifolium, Caulerpa sp., Ulva sp., Eucheuma spinosum, and Gelidium sp. Development Locations are located throughout the waters in the shape of a bay and are by the stipulated water utilization spatial plan. Developing seaweed aqua tourism and institutional arrangements related to seaweed production is necessary.
Insecticide with the active ingredient methomyl interferes with the growth and survival of the jatiumbulan tilapia strain (Oreochromis niloticus) Nurhalisa Nurhalisa; Diana Arfiati; Sri Andayani; Afella Osa; Vina Nur Nadiro
Jurnal Penelitian Pendidikan IPA Vol 9 No 2 (2023): February
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i2.2928

Abstract

Insecticides with the active ingredient methomyl are a class of pesticides used to protect agricultural crops from attack by pests such as insects. The areas that used this insecticide were from the results of previous studies, namely the Musi Rawas Paddy Field Center in South Sumatra and Ngantru District, Tulungagung Regency. However, it is possible that many other areas use it. This study aims to prove that the insecticide with the active ingredient metomyl interferes with the growth and survival of the Jatiumbulan tilapia strain (O. niloticus). The treatment consisted of a control and three exposure concentrations namely 1.8, 4.015 and 6.2 mg/L with 3 repetitions each. Exposure was carried out artificially once in rearing water and observations were made for 4 days (96 hours). In conclusion, the results showed that as the exposure concentration of the insecticide with the active ingredient metomil (Lannate with 25 % methomyl) increased, the stress occurred faster which resulted in decreased growth, survival, time of death, and clinical symptoms. However, the concentration of 6.2 mg/L (treatment C) gave significantly different results in survival parameters compared to the 1.8 and 4.015 mg/L treatment. This is because in treatment C there was stress to death and this condition did not occur in treatment A and B
Immunostimulant Activity of Neem Leaf (A. indica A. Juss) Ethanol Fraction on Tilapia (Oreochromis niloticus) Rika Putri; Sri Andayani; Ating Yuniarti
The Journal of Experimental Life Science Vol. 8 No. 3 (2018)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1110.631 KB) | DOI: 10.21776/ub.jels.2018.008.03.08

Abstract

Intensive cultivation of Tilapia with high stock density and continuous artificial feeding will decrease the water quality and increase the growth of pathogenic bacteria. That situation led to the fish stressed so that the fish become the target of bacterial pathogen attacks. Alternatively, the environmental friendly treatment using herbal immunostimulant to solve that problem is required. The herbal in this study used neem leaf (Azadirachta indica A. Juss). The aim of this study was to evaluate ethanol fraction of neem leaf on immunostimulant activity of Oreochromis niloticus. In this study, the extraction method used maceration. Fractionation methods were using Thin Layer Chromatography (TLC) and Column Chromatography (CC). The biological component was analyzed using Phytochemical, FTIR and GC-MS methods. TLC test showed that ethyl acetate: n-hexane (1:1, v/v) result in brightest luminescence colors on the plates of silica gel under UV light (365 nm). The chromatography column from the ethanol extract of neem leaf (A. indica A. Juss) showed that separation of the fraction was done based on the formation of color, which obtained 5 (five) types of fractions. The results of the phytochemical test for the fraction 4 of neem leaf contains two compounds, i.e. flavonoids (+) and triterpenoids (++). The FTIR test showed that fraction 4 of neem leaves contain a class of terpenoid and flavonoid compounds. GCMS test results on fraction 4 of the neem leaves (A. indica A. Juss) obtained the high peak, which has 4 terpenoid compounds, 1 compound of flavonoid, and 1 type of lauric acid. The total leukocyte of the fish test increased after treated with fraction 4 of neem leaf (A. indica A. Juss) compared with control on day 7 after injection, i.e. 25.29 x 104 cells.mL-1.Keywords: fraction, leukocyte, Neem Leaves, Tilapia, Phytochemicals.
Co-Authors Afella Osa Ating Yuniarti Chih-Hsuan Tseng Diana Arfiati Etika Dwi Rahmawati Heny Suprastyani Mira Leyana Muhamad Firdaus Nunik Cokrowati Nurhalisa Nurhalisa Rika Putri Saou-Lien Wong Vina Nur Nadiro Violando, Wiga Alif Yenny Risjani