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All Journal JSFK (Jurnal Sains Farmasi & Klinis) Pharmaceutical And Biomedical Sciences Journal (PBSJ) Pharmaceutical Sciences and Research (PSR)
Lina Elfita
Program studi Farmasi Fakultas Kedokteran dan Ilmu Kesehatan Universitas Islam Syarif Hidayatullah
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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Purifikasi Inhibitor Atpase/Rna Helikase Virus Japanese Encephalitis Dari Streptomyces chartreusis Elfita, Lina; Ratnakomala, Shanti; Suryadi, Herman; Lisdiyanti, Puspita; Utama, Andi
Majalah Ilmu Kefarmasian Vol. 6, No. 2
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Japanese encephalitis virus (JEV) is a neuropathogenic virus commonly caused cen-tral nervous diseases such as meningitis and severe encephalitis. Although vaccine has been developed, no specific and effective drug is available so far. We previously carried out a screening of inhibitor of JEV RNA helicase, an enzyme that essential for virus replication, from Actinomycetes and found that Streptomyces chartreusis produce the inhibitor of JEV RNA helicase. In this study, an extracellular protein which has inhibition activity on ATPase activity of JEV RNA helicase was purified from supernatant of Streptomyces chartreusis culture by ammonium sulfate pre-cipitation and size exclusion chromatography. SDS-PAGE analysis showed a single band with aproximate molecular mass of 11,4 kDa, suggesting that the inhibitor was successfully purified into a single protein.
Edible Bird’s Nest Extract Reduced Expression of Senescence Markers in Bone Marrow Mesenchymal Stem Cells Elfita, Lina; Wientarsih, Ietje; Sajuthi, Dondin; Bachtiar, Indra; Darusman, Huda Shalahudin
JSFK (Jurnal Sains Farmasi & Klinis) Vol 8 No 1 (2021): J Sains Farm Klin 8(1), April 2021
Publisher : Fakultas Farmasi Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jsfk.8.1.19-26.2021

Abstract

Edible bird’s nest (EBN) is often consumed as a health food due to its suggested health benefits, including anti-aging effects, however the mechanism is still unknown. This study investigated the effect of EBN extract using long term expansion bone marrow-derived mesenchymal stem cells (BMMSCs) as an aging model. Passage 5 (P5) and passage 8 (P8) BMMSCs were treated with EBN extract, and their proliferation, senescence-associated β-galactosidase (SA-β-Gal) activity, and expression of p16INK4a were analyzed. Treatment of BMMSCs with EBN extract decreased population doubling time (PDT) in P5 but not in P8 BMMSCs. In P5 BMMSCs, 200 ppm EBN extract increased BMMSCs proliferation, with PDT reduced by 27.6%. However, 200 ppm EBN extracts did not affect P8 BMMSCs proliferation, although it increased BMMSCs viability. Treatment of P5 and P8 BMMSCs with 200 ppm EBN extract decreased SA-β-Gal activity by 54.8% and 47.1% of the control, respectively (P<0.05). Levels of p16INK4a expression were 5.4-fold lower in P5 BMMSCs treated with 200 ppm EBN extract compared to control (P<0.05). Similarly, treatment of P8 BMMSCs with 200 ppm EBN extract reduced p16INK4a mRNA level by 7.9-fold compared to the control (P<0.05). In order to investigate the pathway of EBN extract inhibition, we further analyzed IL-6 and NF-κB1 expression. Treatment of P5 and P8 BMMSCs with 200 ppm EBN extract reduced IL-6 mRNA levels by 7.9-fold and 2.1-fold of control, respectively (P<0.05). We found that 200 ppm EBN extract reduced NF-κB1 mRNA level approximately 2.4-fold both in P5 and P8 BMMSCs (P<0.05). Thus, EBN extract reduces markers of senescence, indicated by decreased SA-β-Gal activity and p16INK4a mRNA level, and this correlated with reduced messenger RNA levels of the pro-inflammatory factor IL-6 and the transcription factor NF-κB1.
Studi Penambatan Molekuler dan Simulasi Dinamika Molekuler Senyawa Turunan Furanokumarin terhadap Reseptor Estrogen Alfa (ER-α) Sebagai Anti Kanker Payudara Elfita, Lina; Apriadi, Anjas; Supandi, Supandi; Dianmurdedi, Shanifa
JSFK (Jurnal Sains Farmasi & Klinis) Vol 9 No 3 (2022): J Sains Farm Klin 9(3), Desember 2022
Publisher : Fakultas Farmasi Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jsfk.9.3.255-264.2022

Abstract

Kanker payudara menjadi salah satu jenis kanker dengan penderita terbanyak baik di dunia maupun di Indonesia, Reseptor Estrogen Alfa (ER-α) menjadi target utama karena dapat mengatur transkripsi gen dan jalur persinyalan interseluler. Penelitian ini bertujuan untuk menganalisis afinitas dan kestabilan ikatan kompleks ligan senyawa turunan furanokumarin dengan reseptor estrogen alfa. Metode yang digunakan secara in silico atau komputasi yaitu penambatan molekuler menggunakan software AutoDock dan simulasi dinamika molekuler menggunakan software Gromacs. Hasil penambatan molekuler senyawa Bergamottin sebagai senyawa uji paling baik dengan nilai ∆G = -8,98 kkal/mol. Sedangkan ligan pembanding 4-Hydroxytamoxifen dengan nilai ∆G = -11,34 kkal/mol. Hal tersebut menunjukkan bahwa afinitas 4-Hydroxytamoxifen masih lebih baik daripada Bergamottin. Kestabilan ikatan ligan-reseptor dikonfirmasi dengan simulasi dinamika molekuler menunjukkan 4-Hydroxytamoxifen lebih stabil berikatan dengan ER-α berdasarkan parameter Root Mean Square Deviation (RMSD), Root Mean Square Fluctuation (RMSF), Radius of Gyration dan ikatan hidrogen. 4-Hydroxytamoxifen memiliki afinitas dan kestabilannya lebih baik dalam berikatan dengan reseptor estrogen alfa (ER-α) 
Studi Farmakologi Ikan Zebra sebagai Model Obesitas dan Hiperglikemia: Pengembangan Induksi Diet-Induced Obesity Nurfakhrurajab, Irfan; Elfita, Lina; Fitriana, Narti
Pharmaceutical and Biomedical Sciences Journal (PBSJ) Vol 7, No 1 (2025)
Publisher : UIN Syarif Hidayatullah Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/pbsj.v7i1.45453

Abstract

Obesity and hyperglycemia are major risk factors for type 2 diabetes mellitus and various other metabolic disorders. The use of relevant animal models is essential to support pharmacological studies. The aim of this research was to develop an effective DIO (Diet-Induced Obesity) inducer for commercially available zebrafish as a model for obesity and hyperglycemia. Adult zebrafish were induced for obesity and hyperglycemia through administration of a high-fat diet for 4 weeks. The observed parameters included body weight, body length, body mass index (BMI), and blood glucose levels. The results showed that DIO induction significantly increased body weight, BMI, and blood glucose levels (P < 0.05) compared to the control group. In conclusion, zebrafish can be developed as a model for obesity and hyperglycemia through DIO induction, thus potentially serving as an alternative test animal for pharmacological studies of metabolic diseases.
Studi Farmakologi Ikan Zebra sebagai Model Obesitas dan Hiperglikemia: Pengembangan Induksi Diet-Induced Obesity Elfita, Lina; Nurfakhrurajab, Irfan; Fitriana, Narti
Pharmaceutical and Biomedical Sciences Journal (PBSJ) Vol. 7 No. 1 (2025)
Publisher : Pharmaceutical and Biomedical Sciences Journal (PBSJ)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/pbsj.v7i1.45453

Abstract

Obesity and hyperglycemia are major risk factors for type 2 diabetes mellitus and various other metabolic disorders. The use of relevant animal models is essential to support pharmacological studies. The aim of this research was to develop an effective DIO (Diet-Induced Obesity) inducer for commercially available zebrafish as a model for obesity and hyperglycemia. Adult zebrafish were induced for obesity and hyperglycemia through administration of a high-fat diet for 4 weeks. The observed parameters included body weight, body length, body mass index (BMI), and blood glucose levels. The results showed that DIO induction significantly increased body weight, BMI, and blood glucose levels (P < 0.05) compared to the control group. In conclusion, zebrafish can be developed as a model for obesity and hyperglycemia through DIO induction, thus potentially serving as an alternative test animal for pharmacological studies of metabolic diseases
Co-Authors ANDI UTAMA Apriadi, Anjas Dianmurdedi, Shanifa Dondin Sajuthi Herman Suryadi Huda Shalahudin Darusman IETJE WIENTARSIH Indra Bachtiar Narti Fitriana, Narti Nurfakhrurajab, Irfan PUSPITA LISDIYANTI SHANTI RATNAKOMALA Supandi