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Analisis Profil Protein dan Asam Amino Sarang Burung Walet (Collocalia fuchiphaga)Asal Painan Lina Elfita
Jurnal Kimia Valensi Jurnal Valensi Volume 4, No.1, Mei 2014
Publisher : Syarif Hidayatullah State Islamic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/jkv.v4i1.1078

Abstrak Penelitian tentang profil protein sarang burung wallet masih terbatas, terutama sarang burung walet dari Indonesia. Oleh karena itu, penelitian ini bertujuan untuk menganalisa profil protein dan asam amino sarang burung walet yang berasal dari daerah Painan, Kabupaten Pesisir Selatan, Sumatera Barat. Analisis protein dilakukan dengan menggunakan SDS-PAGE, sedangkan analisis asam amino dilakukan dengan menggunakan kromatografi cair kinerja tinggi (KCKT). Analisa ekstrak air sarang burung walet dengan SDS-PAGE menunjukan bahwa sarang burung walet terdiri dari 6 protein. Keenam protein tersebut mempunyai bobot molekul masing-masing 147.2 kDa, 142.6 kDa, 133.4 kDa, 73.3 kDa, 66.2 kDa dan 37.7 kDa. Dari analisa asam amino burung walet dengan KCKT didapatkan 16 asam amino yang terkandung dalam sarang burung wallet, yang terdiri dari 7 jenis asam amino esensial yaitu Histidin (2.31%), Leusin (3.84%), Treonin (3.82%), Valin (3.93%), Metionin (0.48%), Isoleusin (1.80%), Fenilalanine (4.49%) dan 9 asam amino non esensial yaitu Asam Serin (4.56%), Aspartat (4.48%), Arginin (3.93%), Lisin (2.34 %), Prolin (3.64%), Asam glutamate (3.65%), Glisin (1.87%), Alanin (1.31%), Tirosin (3.92%). Serin merupakan asam amino dengan kadar tertinggi (4.56%), diikuti dengan Fenil alanine (4.49%) dan Asam aspartate (4.48%). Kandungan asam amino ini sedikit berbeda dengan kandungan asam amino sarang burung walet dari daerah dan negara lain. Kata kunci: sarang burung walet, protein, asam amino Abstract Study on protein profile of bird nest is still limited particularly protein profile of bird nest from Indonesia has not been reported. Therefore, this study was aimed to analyze protein profile and amino acid composition of bird nest from Painan, Pesisir Selatan Distric, West Sumatra. Protein analysis was performed by SDS-PAGE, and high performance liquid chromatography (HPLC) was used for amino acid analysis. SDS-PAGE analysis showed six bands, which molecular weigh of 147.2 kDa, 142.6 kDa, 133.4 kDa, 73.3 kDa, 66.2 kDa and 37.7 kDa, respectively. On the other hand, HPLC analysis demonstrated that bird nest was composed of 16 amino acids. Seven of them were essential amino acids; histidine (2.31%), leucine (3.84%), threonine (3.82%), valine (3.93%), methionine (0.48%), isoleucine (1.80%), phenylalanine (4.49%), and nine of them were non-essential amino acids; serine (4.56%), aspartic acid (4.48%), arginine (3.93%), lysine (2.34%), proline (3.64%), glutamic acid (3.65%), glycine (1.87%), alanine (1.31%), tyrosine (3.92%). Serine was the highest percentage of amino acid in the bird nest (4.56%), followed by phenylalanine (4.49%) and aspartic acid (4.48%). Composition of amino acid in this bird nest was slightly different with composition of amino acid in bird nest from other area. Keywords : bird nest, protein profile, amino acids

Analisis Profil Protein dan Asam Amino Sarang Burung Walet (Collocalia fuchiphaga) Asal Painan Lina Elfita
Jurnal Sains Farmasi & Klinis Vol 1, No 1 (2014): J Sains Farm Klin 1(1), November 2014
Publisher : Fakultas Farmasi Universitas Andalas

This study was aimed to analyze protein profile and amino acid composition of bird nest from Painan, Pesisir Selatan Distric, West Sumatra. Protein analysis was performed by Sodium Dodecyl Sulphate Polyacrilamide Gel Electrophoresis (SDS-PAGE), meanwhile High Performance Liquid Chromatography (HPLC) was used for analysis of amino acid. Analysis on water extract of bird nest by SDS-PAGE showed six bands which correspond to molecular protein which had molecular weight of 147.2; 142.6; 133.4; 73.3; 66.2; and 37.7 kDa, respectively. HPLC analysis demonstrated that bird nest was composed of 16 amino acids. Seven of them were essential amino acids; histidine (2.31%), leucine (3.84%), threonine (3.82%), valine (3.93%), methionine (0.48%), isoleucine (1.80%), phenylalanine (4.49%), and nine of them were non-essential amino acids; serine (4.56%), aspartic acid (4.48%), arginine (3.93%), lysine (2.34%), proline (3.64%), glutamic acid (3.65%), glycine (1.87%), alanine (1.31%), and tyrosine (3.92%). Serine was the highest percentage of amino acid in the bird nest (4.56%), followed by phenylalanine (4.49%) and aspartic acid (4.48%). The study also showed that composition of amino acid in this bird nest was slightly different with composition of amino acid in bird nest from other area.

Edible Bird’s Nest Extract Reduced Expression of Senescence Markers in Bone Marrow Mesenchymal Stem Cells Lina Elfita; Ietje Wientarsih; Dondin Sajuthi; Indra Bachtiar; Huda Shalahudin Darusman
Jurnal Sains Farmasi & Klinis Vol 8, No 1 (2021): J Sains Farm Klin 8(1), April 2021
Publisher : Fakultas Farmasi Universitas Andalas

Edible bird’s nest (EBN) is often consumed as a health food due to its suggested health benefits, including anti-aging effects, however the mechanism is still unknown. This study investigated the effect of EBN extract using long term expansion bone marrow-derived mesenchymal stem cells (BMMSCs) as an aging model. Passage 5 (P5) and passage 8 (P8) BMMSCs were treated with EBN extract, and their proliferation, senescence-associated β-galactosidase (SA-β-Gal) activity, and expression of p16INK4a were analyzed. Treatment of BMMSCs with EBN extract decreased population doubling time (PDT) in P5 but not in P8 BMMSCs. In P5 BMMSCs, 200 ppm EBN extract increased BMMSCs proliferation, with PDT reduced by 27.6%. However, 200 ppm EBN extracts did not affect P8 BMMSCs proliferation, although it increased BMMSCs viability. Treatment of P5 and P8 BMMSCs with 200 ppm EBN extract decreased SA-β-Gal activity by 54.8% and 47.1% of the control, respectively (P<0.05). Levels of p16INK4a expression were 5.4-fold lower in P5 BMMSCs treated with 200 ppm EBN extract compared to control (P<0.05). Similarly, treatment of P8 BMMSCs with 200 ppm EBN extract reduced p16INK4a mRNA level by 7.9-fold compared to the control (P<0.05). In order to investigate the pathway of EBN extract inhibition, we further analyzed IL-6 and NF-κB1 expression. Treatment of P5 and P8 BMMSCs with 200 ppm EBN extract reduced IL-6 mRNA levels by 7.9-fold and 2.1-fold of control, respectively (P<0.05). We found that 200 ppm EBN extract reduced NF-κB1 mRNA level approximately 2.4-fold both in P5 and P8 BMMSCs (P<0.05). Thus, EBN extract reduces markers of senescence, indicated by decreased SA-β-Gal activity and p16INK4a mRNA level, and this correlated with reduced messenger RNA levels of the pro-inflammatory factor IL-6 and the transcription factor NF-κB1.

Purifikasi Inhibitor Atpase/Rna Helikase Virus Japanese Encephalitis Dari Streptomyces chartreusis Elfita, Lina; Ratnakomala, Shanti; Suryadi, Herman; Lisdiyanti, Puspita; Utama, Andi
Majalah Ilmu Kefarmasian Vol. 6, No. 2
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Japanese encephalitis virus (JEV) is a neuropathogenic virus commonly caused cen-tral nervous diseases such as meningitis and severe encephalitis. Although vaccine has been developed, no specific and effective drug is available so far. We previously carried out a screening of inhibitor of JEV RNA helicase, an enzyme that essential for virus replication, from Actinomycetes and found that Streptomyces chartreusis produce the inhibitor of JEV RNA helicase. In this study, an extracellular protein which has inhibition activity on ATPase activity of JEV RNA helicase was purified from supernatant of Streptomyces chartreusis culture by ammonium sulfate pre-cipitation and size exclusion chromatography. SDS-PAGE analysis showed a single band with aproximate molecular mass of 11,4 kDa, suggesting that the inhibitor was successfully purified into a single protein.

Studi Penambatan Molekuler dan Simulasi Dinamika Molekuler Senyawa Turunan Furanokumarin terhadap Reseptor Estrogen Alfa (ER-α) Sebagai Anti Kanker Payudara Lina Elfita; Anjas Apriadi; Supandi Supandi; Shanifa Dianmurdedi
Jurnal Sains Farmasi & Klinis Vol 9, No 3 (2022): J Sains Farm Klin 9(3), Desember 2022
Publisher : Fakultas Farmasi Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jsfk.9.3.255-264.2022

Kanker payudara menjadi salah satu jenis kanker dengan penderita terbanyak baik di dunia maupun di Indonesia, Reseptor Estrogen Alfa (ER-α) menjadi target utama karena dapat mengatur transkripsi gen dan jalur persinyalan interseluler. Penelitian ini bertujuan untuk menganalisis afinitas dan kestabilan ikatan kompleks ligan senyawa turunan furanokumarin dengan reseptor estrogen alfa. Metode yang digunakan secara in silico atau komputasi yaitu penambatan molekuler menggunakan software AutoDock dan simulasi dinamika molekuler menggunakan software Gromacs. Hasil penambatan molekuler senyawa Bergamottin sebagai senyawa uji paling baik dengan nilai ∆G = -8,98 kkal/mol. Sedangkan ligan pembanding 4-Hydroxytamoxifen dengan nilai ∆G = -11,34 kkal/mol. Hal tersebut menunjukkan bahwa afinitas 4-Hydroxytamoxifen masih lebih baik daripada Bergamottin. Kestabilan ikatan ligan-reseptor dikonfirmasi dengan simulasi dinamika molekuler menunjukkan 4-Hydroxytamoxifen lebih stabil berikatan dengan ER-α berdasarkan parameter Root Mean Square Deviation (RMSD), Root Mean Square Fluctuation (RMSF), Radius of Gyration dan ikatan hidrogen. 4-Hydroxytamoxifen memiliki afinitas dan kestabilannya lebih baik dalam berikatan dengan reseptor estrogen alfa (ER-α)

Edible Bird’s Nest Extract Reduced Expression of Senescence Markers in Bone Marrow Mesenchymal Stem Cells Elfita, Lina; Wientarsih, Ietje; Sajuthi, Dondin; Bachtiar, Indra; Darusman, Huda Shalahudin
JSFK (Jurnal Sains Farmasi & Klinis) Vol 8 No 1 (2021): J Sains Farm Klin 8(1), April 2021
Publisher : Fakultas Farmasi Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jsfk.8.1.19-26.2021

Edible birdâ€™s nest (EBN) is often consumed as a health food due to its suggested health benefits, including anti-aging effects, however the mechanism is still unknown. This study investigated the effect of EBN extract using long term expansion bone marrow-derived mesenchymal stem cells (BMMSCs) as an aging model. Passage 5 (P5) and passage 8 (P8) BMMSCs were treated with EBN extract, and their proliferation, senescence-associated Î²-galactosidase (SA-Î²-Gal) activity, and expression of p16INK4a were analyzed. Treatment of BMMSCs with EBN extract decreased population doubling time (PDT) in P5 but not in P8 BMMSCs. In P5 BMMSCs, 200 ppm EBN extract increased BMMSCs proliferation, with PDT reduced by 27.6%. However, 200 ppm EBN extracts did not affect P8 BMMSCs proliferation, although it increased BMMSCs viability. Treatment of P5 and P8 BMMSCs with 200 ppm EBN extract decreased SA-Î²-Gal activity by 54.8% and 47.1% of the control, respectively (P<0.05). Levels of p16INK4a expression were 5.4-fold lower in P5 BMMSCs treated with 200 ppm EBN extract compared to control (P<0.05). Similarly, treatment of P8 BMMSCs with 200 ppm EBN extract reduced p16INK4a mRNA level by 7.9-fold compared to the control (P<0.05). In order to investigate the pathway of EBN extract inhibition, we further analyzed IL-6 and NF-ÎºB1 expression. Treatment of P5 and P8 BMMSCs with 200 ppm EBN extract reduced IL-6 mRNA levels by 7.9-fold and 2.1-fold of control, respectively (P<0.05). We found that 200 ppm EBN extract reduced NF-ÎºB1 mRNA level approximately 2.4-fold both in P5 and P8 BMMSCs (P<0.05). Thus, EBN extract reduces markers of senescence, indicated by decreased SA-Î²-Gal activity and p16INK4a mRNA level, and this correlated with reduced messenger RNA levels of the pro-inflammatory factor IL-6 and the transcription factor NF-ÎºB1.

Studi Penambatan Molekuler dan Simulasi Dinamika Molekuler Senyawa Turunan Furanokumarin terhadap Reseptor Estrogen Alfa (ER-Î±) Sebagai Anti Kanker Payudara Elfita, Lina; Apriadi, Anjas; Supandi, Supandi; Dianmurdedi, Shanifa
JSFK (Jurnal Sains Farmasi & Klinis) Vol 9 No 3 (2022): J Sains Farm Klin 9(3), Desember 2022
Publisher : Fakultas Farmasi Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jsfk.9.3.255-264.2022

Kanker payudara menjadi salah satu jenis kanker dengan penderita terbanyak baik di dunia maupun di Indonesia, Reseptor Estrogen Alfa (ER-Î±) menjadi target utama karena dapat mengatur transkripsi gen dan jalur persinyalan interseluler. Penelitian ini bertujuan untuk menganalisis afinitas dan kestabilan ikatan kompleks ligan senyawa turunan furanokumarin dengan reseptor estrogen alfa. Metode yang digunakan secara in silico atau komputasi yaitu penambatan molekuler menggunakan software AutoDock dan simulasi dinamika molekuler menggunakan software Gromacs. Hasil penambatan molekuler senyawa Bergamottin sebagai senyawa uji paling baik dengan nilai âˆ†G = -8,98 kkal/mol. Sedangkan ligan pembanding 4-Hydroxytamoxifen dengan nilai âˆ†G = -11,34 kkal/mol. Hal tersebut menunjukkan bahwa afinitas 4-Hydroxytamoxifen masih lebih baik daripada Bergamottin. Kestabilan ikatan ligan-reseptor dikonfirmasi dengan simulasi dinamika molekuler menunjukkan 4-Hydroxytamoxifen lebih stabil berikatan dengan ER-Î± berdasarkan parameter Root Mean Square Deviation (RMSD), Root Mean Square Fluctuation (RMSF), Radius of GyrationÂ dan ikatan hidrogen. 4-Hydroxytamoxifen memiliki afinitas dan kestabilannya lebih baik dalam berikatan dengan reseptor estrogen alfa (ER-Î±)Â

Studi Farmakologi Ikan Zebra sebagai Model Obesitas dan Hiperglikemia: Pengembangan Induksi Diet-Induced Obesity Nurfakhrurajab, Irfan; Elfita, Lina; Fitriana, Narti
Pharmaceutical and Biomedical Sciences Journal (PBSJ) Vol 7, No 1 (2025)
Publisher : UIN Syarif Hidayatullah Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/pbsj.v7i1.45453

Obesity and hyperglycemia are major risk factors for type 2 diabetes mellitus and various other metabolic disorders. The use of relevant animal models is essential to support pharmacological studies. The aim of this research was to develop an effective DIO (Diet-Induced Obesity) inducer for commercially available zebrafish as a model for obesity and hyperglycemia. Adult zebrafish were induced for obesity and hyperglycemia through administration of a high-fat diet for 4 weeks. The observed parameters included body weight, body length, body mass index (BMI), and blood glucose levels. The results showed that DIO induction significantly increased body weight, BMI, and blood glucose levels (P < 0.05) compared to the control group. In conclusion, zebrafish can be developed as a model for obesity and hyperglycemia through DIO induction, thus potentially serving as an alternative test animal for pharmacological studies of metabolic diseases.

Efektivitas Ekstrak Daun Pepaya (Carica papaya L.) sebagai Wound Healing Menggunakan Ikan Zebra Danio rerio Hamilton, 1822 Yang Diinduksi Hiperglikemia sebagai Model Aini, Falihah Nur; Elfita, Lina; Fitriana, Narti
Pharmaceutical and Biomedical Sciences Journal (PBSJ) Vol. 6 No. 1 (2024)
Publisher : Pharmaceutical and Biomedical Sciences Journal (PBSJ)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/pbsj.v6i1.38957

Papaya leaves are known to have antidiabetic and wound-healing activities because they contain flavonoids, alkaloids, tannins, and saponins. Tests of the activity of papaya leaf extract in mouse models have been widely conducted. In this study, the wound healing activity of papaya leaves was carried out using zebrafish as a model, because of their ability to regenerate and 70% similarity to human glucose metabolism. This study aimed to analyze the potential of zebrafish as a model for healing diabetic wounds using 96% ethanol extract from papaya leaves. Zebrafish were divided into six groups, namely normal control, negative control, positive, and papaya leaf extract 125, 187.5, and 250 ppm. The parameters observed were a decrease in blood sugar levels, regeneration of the zebrafish caudal fin, and survival rate of the fish. The results showed that there was an effect of administering papaya leaf extract at doses of 125, 187.5, and 250 ppm respectively to reduce blood sugar levels by an average of 42.67; 44.33; and 72 mg/dL, an increase in fin length of 1; 1.3; 1 mm, and survival rate with a percentage of 91%, 91%, 64%. It was concluded that zebrafish could be used as a test model for diabetic wound healing. Apart from that, the use of 96% ethanol extract from papaya leaves affects the healing of diabetic wounds. Of the 3 doses given with 96% ethanol extract of papaya leaves, the best dose for healing diabetic wounds was 187.5 ppm.

Studi Farmakologi Ikan Zebra sebagai Model Obesitas dan Hiperglikemia: Pengembangan Induksi Diet-Induced Obesity Lina Elfita; Irfan Nurfakhrurajab; Narti Fitriana
Pharmaceutical and Biomedical Sciences Journal (PBSJ) Vol. 7 No. 1 (2025)
Publisher : Pharmacy Department, Faculty of Health Sciences, UIN Syarif Hidayatullah Jakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/pbsj.v7i1.45453

Obesity and hyperglycemia are major risk factors for type 2 diabetes mellitus and various other metabolic disorders. The use of relevant animal models is essential to support pharmacological studies. The aim of this research was to develop an effective DIO (Diet-Induced Obesity) inducer for commercially available zebrafish as a model for obesity and hyperglycemia. Adult zebrafish were induced for obesity and hyperglycemia through administration of a high-fat diet for 4 weeks. The observed parameters included body weight, body length, body mass index (BMI), and blood glucose levels. The results showed that DIO induction significantly increased body weight, BMI, and blood glucose levels (P < 0.05) compared to the control group. In conclusion, zebrafish can be developed as a model for obesity and hyperglycemia through DIO induction, thus potentially serving as an alternative test animal for pharmacological studies of metabolic diseases

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