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All Journal HAYATI Journal of Biosciences Medical Journal of Indonesia Indonesian Journal of Pharmaceutical Science and Technology Indonesian Journal of Biotechnology Warta IHP (Warta Industri Hasil Pertanian) Indonesian Journal of Chemistry JFIOnline Jurnal Ilmu Kefarmasian Indonesia Jurnal Aisyah : Jurnal Ilmu Kesehatan Jurnal Locus Penelitian dan Pengabdian Journal of Applied Agricultural Science and Technology Pharmaceutical Sciences and Research (PSR) Eduvest - Journal of Universal Studies Ezra Science Bulletin
Herman Suryadi
Department Of Pharmacy, Faculty Of Pharmacy, Universitas Indonesia, Jakarta, Indonesia
Aerospace Engineering Agriculture, Biological Sciences & Forestry Arts Humanities Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Electrical & Electronics Engineering Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Veterinary Other

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Quantitative analysis of lactose and lactulose in urine by high performance liquid chromatography for determination of intestinal lactase activity Dharmasetiawani, Nani; Suryadi, Herman; Firmansyah, Agus
Medical Journal of Indonesia Vol 12, No 1 (2003): January-March
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (231.365 KB) | DOI: 10.13181/mji.v12i1.84

Abstract

Determination of intestinal lactase activity is directly done by measuring its activity in intestinal epithelium. This is an invasive method and ethically can not be done in healthy infants. Indirectly, determination of lactase activity, stated as excretion and ingestion ratio of lactose and lactulose, needs 30 hours hospitalized infants. The aim of this study was to look for a method for determination of lactase activity which is not invasive and not necessary hospitalized. Using this method  lactose and lactulose were given as a single oral load after 2 hours fasting. Urine were collected for 5 hours starting from consuming sugar solution and then lactose and lactulose concentration in the urine were measured by High Performance Liquid Chromatography. The results showed single oral load of lactose and lactulose can be used for determination of lactase activity in infant and the infants were observed only for 7 hours. (Med J Indones 2003; 12: 8-12) Keywords: Lactase activity, Lactulose, Lactose, High Performance Liquid Chromatography
(Catechin and Epicatechin Analysis in Cacao Bean and Cacao products Using Liquid Chromatography Mass Spectrometry) -, Subagja; -, Harmita; Suryadi, Herman; Junaidi, Lukman
Warta Industri Hasil Pertanian Vol 26, No 02 (2009)
Publisher : Balai Besar Industri Agro

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Abstract

Cacao beans processing into product may affect active compound contents in the final products, especially catechin and  epicatechin. Temperature treatments during cacao processing can induce epimerization reaction of (-)-epicatechin to be (-)-catechin. Therefore, catechin and epicatechin ratio in the samples would be change. The aim of the was to know imfluence during cacao beans processing, especially cathechin and epicatechin concentration. Samples taken during the process were cacao nib, cacao mass, cacao powder, and butter. Analysis of catechin and epicatechin in samples was carried out by liquid chromatography mass spectrometry. Analytical for catechin were using mobile phase A (0.1 %formic acid in deionized water) and mobile phase B (acetonitril-methanol = 50:50) at flow rate of 0.5 ml/min. gradient elution were set at 0 minutes (10%B), 15 minutes (35%B), 20 minutes (40%B), 30 minutes (50% B), 35 minutes (60% B), and 35.1 minutes (105 B). Mass sprectrometer was set at ESI voltage (-) 3500 volt, desolvation temperature 300 oC, nubelizer pressure 50 psi, desolvation gas 10L/min, and fragmentor voltage (-) 160 volt. Results of the research showed that of catechin and cacao nib, cacao mass and cacao powder were 1:21,7; 1:20,0; and respectively. Heat treatments during cacao mass processing sowed a decrease tendency of catechin and epicatechin concentrations.
OPTIMASI DAN VALIDASI METODE ANALISIS ASAM NIKOTINAT SERTA STABILITAS INOSITOL HEKSANIKOTINAT Harahap, Yahdiana; Suryadi, Herman; Wardatun, Sri
JFIOnline | Print ISSN 1412-1107 | e-ISSN 2355-696X Vol 6, No 1 (2012)
Publisher : Indonesian Research Gateway

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Abstract

Nicotinic acid is a therapeutic agent for treatment atherosclerosis. Inositol hexanicotinate isan agent that can be hydrolyzed with release nicotinic acid. The low level of free nicotinicacid from inositol hexanicotinate in blood, it’s the reason why it needs method analysis withhigh sensitive and selective. The aims of this research were to optimize and validationmethod analysis nicotinic acid and stability study of inositol hexanicotinate by highperformance liquid chromatography. The method was optimated with variation compositionmobile phase, variation flow rate and optimation process exctraction. Condition analysiswere optimum with use a Kromasil column (250 mm x 4,6 mm) RP, mobile phase mixeddipotassium hydrogen phosphate and potassium dihydrogen phosphate 10 mM containingtetrabuthylammonium bromide 5 mM pH 7 with acetonitril (100:9), flow rate 0,8 ml/minute,with internal standard coffein in 263 nm wave lenght. The standard curve was linear over aconcentration range 124,84 to 5000 ng/ml of nicotinic acid in plasma. The HPLC method wasvalidated with accuracy -6,8779 to 3,09 %, precision 0,3 to 3,71 % and recovery 93,12 -103,09 %. The results of a stability study indicated that inositol hexanicotinate was unstablein plasma samples, but was stable in 0,6 M perchloric acid for to 24 hour at 40C. ABSTRAKAsam nikotinat merupakan obat yang dapat digunakan untuk ateroskerosis dan inositolheksanikotinat merupakan senyawa yang dapat terhidrolisis melepaskan asam nikotinat.Penelitian ini bertujuan untuk optimasi dan validasi metode analisis asam nikotinat, sertamenentukan stabilitas inositol heksanikotinat menggunakan kromatografi cair kinerja tinggi.Kondisi analisis yang optimum diperoleh dengan menggunakan kolom Kromasil (250 mm x4,6 mm) RP, fase gerak campuran kalium dihidrogen fosfat dan dikalium hidrogen fosfat 10mM yang mengandung tetrabutilammonium bromida 5 mM pH 7 dengan asetonitril (100:9),kecepatan alir 0,8 ml/menit, dengan baku dalam kafein, yang dideteksi pada panjanggelombang 263 nm. Kurva kalibrasi linier dari 124,84 sampai 5000 ng/ml. Hasil validasimetode menunjukkan akurasi -6,87 hingga 3,09 %, presisi 0,3 hingga 3,71 % dan perolehankembali 93,12 hingga 103,09%. Hasil uji stabilitas menunjukkan bahwa inositolheksanikotinat tidak stabil dalam plasma tetapi stabil dalam asam perklorat 0,6 M padapenyimpanan 40C selama 24 jam.
Produksi Senyawa Kuinina dengan Fermentasi Mikroba Endofit Tanaman Cinchona pubescens Vahl. Esti Mumpuni; Herman Suryadi; Partomuan Simanjuntak
JURNAL ILMU KEFARMASIAN INDONESIA Vol 3 No 2 (2005): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

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Abstract

This study deals with endophytic microbes, known as a potential source of secondary metabolites. The microbes (F) were isolated from Cinchona pubescens Vahl and fermented in Phoma media, observing the pH, biomass growth and secondary metabolites of alkaloids for 14 days. The result obtained showed that the pH was between 4 and 5, the biomass growth relative stable after 8 days and the production of alkaloids reached its maximum also after 8 days (32.81 mg/l).
Pharmacokinetic Study and Incurred Sample Stability of Esomeprazole in Dried Blood Spot Sample Using High Performance Liquid Chromatography-Photodiode Array Yahdiana Harahap; Anja Tamabri; Vicha Vicha; Herman Suryadi; Sunarsih Sunarsih; Callista Andinie Mulyadi
Indonesian Journal of Chemistry Vol 20, No 5 (2020)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (295.088 KB) | DOI: 10.22146/ijc.42404

Abstract

In the past years, Esomeprazole (EMP) was analyzed in human plasma samples, which still has stability issues; thus, the new biosampling technique known as Dried Blood Spot (DBS) might solve the issue. This research aims to evaluate the incurred sample stability of esomeprazole in dried blood spot using high performance liquid chromatography-photodiode array with lansoprazole as an internal standard. The analytical separation was performed on a C-18 column (Waters, Sunfire™ 5 μm; 250 × 4.6 mm) at 40 °C. The mobile phase used was acetonitrile–phosphate buffer pH 7.6 (40:60% v/v) with a flow rate of 1.00 mL/min; and was detected at 300 nm. The analyte was extracted from dried blood spot by methanol. Incurred sample stability was evaluated from 6 healthy subjects on day 0, 7, 14, and 28, respectively. This method was linear in the range concentration of 70–1400 ng/mL with r > 0.98. Pharmacokinetic study shows that the average of AUC0–t of EMP in the DBS sample was 1765.41 ngh/mL. The highest percent difference value of esomeprazole’s incurred samples stability on day 7, 14, and 28 from 6 healthy subjects were 9.81%. This result fulfilled the acceptance criteria, which is the percent difference should not be greater than 20%, and 67% of total samples have to fulfill the criteria. The incurred sample stability result showed that esomeprazole was stable in the DBS sample at least until 28 days with the highest value of percent difference is 9.81%.
Uji Efektivitas Antimikroba Beberapa Merek Dagang Pembersih Tangan Antiseptik Radji, Maksum; Suryadi, Herman; Ariyanti, Dessy
Majalah Ilmu Kefarmasian Vol. 4, No. 1
Publisher : UI Scholars Hub

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Abstract

In Modern life, peoples are more conscious to keep their healthy. One of the way to keep our body healthy is by keeping our hand clean. Washing hands with soap and water, sometimes is unpractical since it need water to rinse off the soap. Prod-uct innovation has been developed to overcome the unpractical side, which is called antiseptic hand wash or hand sanitizer. This study has been done to evaluate the antimicrobial activity of some hand sanitizer products marketed in Jakarta. The re-sult showed that alcohol-based hand sanitizer and triclosan-based hand sanitizer used in this experiment were significantly reduce bacterial counts on hands, but not all of the samples are effective in reducing fungal counts on hands.
Pemeriksaan Bakteriologis Air Minum Isi Ulang Di Beberapa Depo Air Minum Isi Ulang Di Daerah Lenteng Agung dan Srengseng Sawah Jakarta Selatan Radji, Maksum; Oktavia, Heria; Suryadi, Herman
Majalah Ilmu Kefarmasian Vol. 5, No. 2
Publisher : UI Scholars Hub

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Abstract

Recently, refilled drinking water stores are flourishing in the some cities of Indonesia. This research tries to find out the quality of refilled drinking water at some shop in Jagakarsa, South Jakarta. The samples of refilled drinking water were taken from thir-teen shops around Lenteng Agung and Srengseng Sawah, Jagakarsa area. The bacte-riological test of refilled drinking water was to detect the availability of Coliform bacteria and identification of some bacterial pathogens such as Escherichia coli, Sal-monella, Staphylococcus aureus, Clostridium perfringens and Pseudomonas aeruginosa. It had been concluded that two of thirteen samples of refilled drinking water had the total number of bacteria above of the limit number according to the standard about the quality and requirement of drinking water. Four of the thirteen samples contain Sta-phylococcus aureus and none of the samples contain Escherichia coli, Salmonella, Clostridium perfringens and Pseudomonas aeruginosa.
Purifikasi Inhibitor Atpase/Rna Helikase Virus Japanese Encephalitis Dari Streptomyces chartreusis Elfita, Lina; Ratnakomala, Shanti; Suryadi, Herman; Lisdiyanti, Puspita; Utama, Andi
Majalah Ilmu Kefarmasian Vol. 6, No. 2
Publisher : UI Scholars Hub

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Abstract

Japanese encephalitis virus (JEV) is a neuropathogenic virus commonly caused cen-tral nervous diseases such as meningitis and severe encephalitis. Although vaccine has been developed, no specific and effective drug is available so far. We previously carried out a screening of inhibitor of JEV RNA helicase, an enzyme that essential for virus replication, from Actinomycetes and found that Streptomyces chartreusis produce the inhibitor of JEV RNA helicase. In this study, an extracellular protein which has inhibition activity on ATPase activity of JEV RNA helicase was purified from supernatant of Streptomyces chartreusis culture by ammonium sulfate pre-cipitation and size exclusion chromatography. SDS-PAGE analysis showed a single band with aproximate molecular mass of 11,4 kDa, suggesting that the inhibitor was successfully purified into a single protein.
Amobilisasi Sel Lactobacillus Acidophilus FNCC116 Untuk Demineralisasi Limbah Kulit Udang Dalam Pengolahan Kitin Betha, Ofa Suzanti; Setyahadi, Siswa; Suryadi, Herman
Majalah Ilmu Kefarmasian Vol. 6, No. 3
Publisher : UI Scholars Hub

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Abstract

Chitin, a homopolimer, is the most abundant renewable natural resources after cellulose. Chitin and its derivatives hold many applications in agriculture, textile, pharmacy and medic. Chitin that extracted from waste shrimp shells by biological fermentation has better quality than chemical procees. Demineralization of chitin by biological procees use lactic acid as product of fermentation. Deproteinization of chitin use proteolytic activity of enzyme that produce by bacteria in fermentation. Lacto-bacillus acidophilus FNCC116 has been immobilized by entrapment methods and 2% sodium alginate in 0,2 M CaCl2 as the matric . The ability of immobilized Lacto-bacillus acidophilus FNCC116 cell in fermentation was tested. The fermentation that was carried out in medium which consist of 6% glukosa, 1,5% yeast extract, 0,003% MnSO4 0,003% FeSO4.7H2O, 0,02% MgSO4.7H2O and has been producted 2,24% lactic acid. Demineralization of waste shrimp shell with 30% immobilized Lactobacillus acidophilus FNCC116 cell has successfully decreased ash content tol 1,18% and produced lactic acid maximum 2.24%. Immobilization of Lactobacillus acidophilus FNCC116 cell promised an efficient method in bioproceesing of chitin recovery.
Analisis Formalin dalam Sampel Ikan dan Udang Segar dari Pasar Muara Angke Suryadi, Herman; Kurniadi, Maryati; Melanie, Yuanki
Majalah Ilmu Kefarmasian Vol. 7, No. 3
Publisher : UI Scholars Hub

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Abstract

The use of formalin as a food additive has been prohibited by the ministry of health as stipulated in The Indonesia Ministry of Health Regulations No.722/Menkes/Per/IV/88. However, in recent years the Indonesian authorities have found trace amounts of formalin as a preservative in perishable foods such as fish and shrimp. The aim of this research is to identify the use of formalin in fresh fish and shrimp samples sold in Muara Angke Market as the fresh seafood market in Jakarta. The first step of this research was formalin identification in fish and shrimp samples and continued by quantitative analysis to assure the results obtained. Qualitative determination of formalin was carried out by Schryver reagent and the quantitative determination was carried out pectrophotometrically using Nash reagent. Validation of UV-Vis spectrophotometric method for determination of formalin showed that Nash reagent was suitable to determine formalin. The limit of detection, limit of quantitation, and coefficient of variation for formalin were 0,0102 mg/L, 0,0341 mg/L, and 0,09%, respectively. Recovery of formalin in fish samples was 89,79-109,58% and shrimp samples was 82,11-97,76%. Qualitative determination in six fish samples and six shrimp samples showed negative results and the quantitative analysis confirmed that formalin was not found in the fresh fish and shrimp samples from Muara Angke Market.
Co-Authors -, Harmita . Azizahwati . Azizahwati . Harmita . Hayun Abdul Mun'ím Agus Firmansyah Ambarsari, Christy ANDI UTAMA Andi Utama Andiri Niza Syarifah Anja Tamabri Arif Arrahman Arif Arrahman Arif Arrahman, Arif Arifianti, Ayun Erwina Arry Yanuar Berna Elya Callista Andinie Mulyadi Chairunissa, Ananda Hanny Dessy Ariyanti Dessy Ariyanti Esti Mumpuni Forestrania, Roshamur Cahyan Fready SIP Fuad, Asrul Muhammad Hayun . Hayun Hayun Hayun Hayun Hayun Hayun, Hayun Heria Oktavia Heria Oktavia, Heria Irianti, Marina Ika Jantan, Ibrahim Junaidi, Lukman Kamaruzzaman, Mohd Amir Larosa, Febri LS Lina Elfita Lina Elfita Luh Putu Ratna Sundari Makau, Juliann Nzembi Maksum Radji Maksum Radji Maksum Radji MARIA BINTANG Maryati Kurniadi Maryati Kurniadi Melanie, Yuanki Nafi’ah, Nadya Ismi Nani Dharmasetiawani Ofa Suzanti Betha Partomuan Simanjuntak Prasetio, Nisrina Putri PUSPITA LISDIYANTI Putri Mulia, Ocha Rahmadi, Agus Rahmasari, Ratika Rifki Anshory Hendri Sembiring, Enny Rimita Septiarini, Tri Hastuti SHANTI RATNAKOMALA Shanti Ratnakomala Siburian, Eva Siswa Setyahadi Siswa Setyahadi Sri Wardatun Subagja ., Subagja Sunarsih Sunarsih Susandra Jaya Vicha Vicha Yahdiana Harahap Yahdiana Harahap Yodifta Astiningrum Yodifta Astriningrum Yodifta Astriningrum, Yodifta