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All Journal BERITA BIOLOGI Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Pharmaceutical Sciences and Research (PSR)
SHANTI RATNAKOMALA
Pusat Penelitian Bioteknologi-LIP
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology

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AKTIVITAS ANTIBAKTERI AKTINOMISETES LAUT DARI PULAU ENGGANO [Antibacterial activity of marine actinomycetes from Enggano Island] Ratnakomala, Shanti; Apriliana, Pamella; Fahrurrozi, Fahrurrozi; Lisdiyanti, Puspita; Kusharyoto, Wien
BERITA BIOLOGI Vol 15, No 3 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3376.649 KB) | DOI: 10.14203/beritabiologi.v15i3.2258

Abstract

Marine actinomycetes were isolated from mangrove sediment from the coast in Enggano, Bengkulu Province, Indonesia using the medium NBRC No. 802 modified by the addition of 2% NaCl. A total of 29 isolates of actinomycetes were isolated from three mangrove sediment samples and evaluated their potential to produce bioactive metabolites. Screening of 29 isolates marine actinomycetes isolates were performed against three bacterial pathogens had been done. Bacteria test used was Escherichia coli NBRC 14 237, Staphylococcus aureus NBRC13 276, and Bacillus subtilis NBRC 3134. Screening result showed that seven isolates have inhibitory effects against bacteria test and 22 other isolates have no inhibition. Of the seven isolates, one isolate has inhibitory effect against the growth of Gram-negative bacteria Escherichia coli, while six other isolates inhibit Gram-positive bacteria Bacillus subtilis and Staphilococcus aureus. It was concluded that, of the 29 isolates conducted in the experiment, seven isolates produce antibacterial compounds on agar medium. Molecular identification of 23 isolates were identified based on  the gene 16S RNA sequences showed that 22 isolates belong to the genus Streptomyces and one strain belongs to the genus Dermacoccus.
Purifikasi Inhibitor Atpase/Rna Helikase Virus Japanese Encephalitis Dari Streptomyces chartreusis Elfita, Lina; Ratnakomala, Shanti; Suryadi, Herman; Lisdiyanti, Puspita; Utama, Andi
Majalah Ilmu Kefarmasian Vol. 6, No. 2
Publisher : UI Scholars Hub

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Abstract

Japanese encephalitis virus (JEV) is a neuropathogenic virus commonly caused cen-tral nervous diseases such as meningitis and severe encephalitis. Although vaccine has been developed, no specific and effective drug is available so far. We previously carried out a screening of inhibitor of JEV RNA helicase, an enzyme that essential for virus replication, from Actinomycetes and found that Streptomyces chartreusis produce the inhibitor of JEV RNA helicase. In this study, an extracellular protein which has inhibition activity on ATPase activity of JEV RNA helicase was purified from supernatant of Streptomyces chartreusis culture by ammonium sulfate pre-cipitation and size exclusion chromatography. SDS-PAGE analysis showed a single band with aproximate molecular mass of 11,4 kDa, suggesting that the inhibitor was successfully purified into a single protein.
The Marine Actinobacterium Streptomyces sp. BTA 1-131 as a Potential Producer of Anti-Nontuberculous Mycobacterial (Anti-NTM) Paramita, Ni Luh Putu Vidya; Atikana, Akhirta; Sukmarini, Linda; Untari, Febriana; Prayoga, Muhammad Bagas; Maulidia, Faiqoh Nur; Ratnakomala, Shanti; Lisdiyanti, Puspita; Nuryastuti, Titik; Hertiani, Triana
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 19, No 3 (2024): December 2024
Publisher : :Agency for Marine and Fisheries Research and Human Resources, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.882

Abstract

Nontuberculous mycobacteria (NTM) are environmental microorganisms, also known as opportunistic pathogens, found in patients with pulmonary tuberculosis. The emergence of antibiotic resistance is increased by prolonged antibiotic treatment for NTM infections. Therefore, alternative sources of new antibiotics are essential for the treatment of NTM infections. A marine actinobacterium, Streptomyces sp. BTA 1-131, isolated from a marine sponge, Melophlus sarasinorum, has been reported as a potential source of antibacterials and anticancer agents. The present study aimed to investigate the potential of Streptomyces sp. BTA 1-131 against two NTMs: Mycobacterium smegmatis and Mycobacterium fortuitum. Streptomyces sp. BTA 1-131 was fermented in three cultivation media (SYP, ISP2, and YS), and the secondary metabolites were extracted using methanol. The bioactivity screening showed inhibition of all methanolic extracts against the growth of M. smegmatis and M. fortuitum. The methanolic extract, which could inhibit both mycobacteria, was a crude extract derived from SYP liquid medium fermentation. The isolated compounds in this study were preliminarily identified using thin-layer chromatography (TLC). The TLC results showed different potential compounds in the crude extracts of Streptomyces sp. BTA 1-131 also highlighted the impact of the fermentation medium on the production of metabolites from Streptomyces. This study also added knowledge about the importance of the Indonesian marine actinobacterium Streptomyces sp. BTA 1-131 as a promising producer of anti-NTM compounds.
Co-Authors Abd. Rasyid Syamsuri ANDI UTAMA ANDO KATSUHIKO Anja Meryandini Apriliana, Pamella Atikana, Akhirta Bambang Prasetya Endang Sukara GINA KARTINA Herman Suryadi Kusharyoto, Wien Lina Elfita LINDA SUKMARINI Lisdayanti, Puspita Maggy T Suhartono Maulidia, Faiqoh Nur MISA OTOGURO Ni Luh Putu Vidya Paramita Prayoga, Muhammad Bagas PUSPITA LISDIYANTI RONI RIDWAN Susanti, Agustina Eko Titik Nuryastuti TOMOHIKO TAMURA Triana Hertiani Trisanti Anindyawati, Trisanti Untari, Febriana WIBOWO MANGUNWARDOYO Yantyati Widyastuti YOPI YOPI YULIN LESTARI