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All Journal Jurnal Pangan dan Agroindustri BIOTROPIC The Journal of Tropical Biology Jurnal SainHealth
Andita Ayu Mandasari
Universitas Maarif Hasyim Latif
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Immunology & microbiology Medicine & Pharmacology

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Perubahan Kualitas Sperma Mencit (Mus musculus) Yang Terpapar Asap Rokok Elektrik Andita Ayu Mandasari; Siti Nur Asiyah; Kurnia Lintang
Biotropic : The Journal of Tropical Biology Vol. 3 No. 2 (2019): Biotropic, Volume 3 Nomor 2, 2019
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (677.897 KB) | DOI: 10.29080/biotropic.2019.3.2.122-128

Abstract

Smoking is one of the habits that can affect human health. One type of cigarette that is currently the choice is electric cigarettes. The e-cigarette content includes nicotine, propylene glycol, flavorings and glycerin. The purpose of this study was to determine the effect of exposure to e-cigarette smoke on mice strain balb / c with the parameters of the number of spermatozoa. This study used 15 male mice which were 8-10 weeks old and weighed 25-30 grams of male mice which were divided into 3 treatment groups namely control group, treatment group one and treatment group two. In the control group no exposure to cigarette smoke was carried out while in the treatment group one was exposed to cigarette smoke in mice with a dose of 0 mg nicotine and the second treatment group was exposed to e-cigarette smoke at a dose of 18 mg nicotine. Electric cigarette smoke exposure is carried out for 30 days using a suction device from a syringe. The exposure of cigarette smoke is carried out every day with successive doses of 20 times as much as 3 times with a time lag of 10 minutes (5 minutes the chamber is closed and 5 minutes of the chamber is opened). The results showed that the average number of spermatozoa in the control group was 16.816 million / ml. The average number of spermatozoa in treatment one was 10.432 million / ml and the average number of spermatozoa in treatment two was 5.234 million / ml. This shows that exposure to cigarette smoke can reduce the number of spermatozoa from the control group to the first treatment by 42.56% and the second treatment shows a decrease from the first group by 34.65%.
PEMANFAATAN EKSTRAK BUNGA SEPATU (Hibiscus rosa-sinensis L.) SEBAGAI ALTERNATIF PEWARNA ALAMI SEDIAAN SITOLOGI PENGGANTI EOSIN PADA PENGECATAN DIFF QUIK Lukmatul Mutoharoh; Setyo Dwi Santoso; Andita Ayu Mandasari
Jurnal SainHealth Vol 4, No 2 (2020): September 2020
Publisher : Faculty of Health Sciences Universitas Maarif Hasyim Latif

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51804/jsh.v4i2.770.21-26

Abstract

Bunga sepatu (Hibiscus rosa-sinensis L.) merupakan tanaman semak kelompok Malvaceae yang berasal dari Asia Timur dan banyak dimanfaatkan sebagai pagar hidup maupun tanaman hias didaerah subtropis dan tropis. Bunga sepatu dulu banyak digunakankan sebagai pewarna alami untuk makanan. Bunga sepatu mengandung pigmen warna antosianin. Pemanfaatan antosianin telah banyak dilakukankan salah satunya pewarna produk makanan maupun minuman. Pewarna alami antosianin semakin banyak diminati karena dapat mengurangi penggunaan pewarna sintetik yang memiliki sifat toksik serta tidak ramah lingkungan. Tujuan penelitian ini adalah untuk mengetahui pemanfaatan ekstrak bunga sepatu (Hibiscus rosa-sinensis L.) sebagai pewarna alami pengganti eosin pada pengecatan diff quik. Sampel yang digunaakan untuk membuat sediaan sitologi berasal dari kerokan mukosa bukal. Bagian kelopak bunga sepatu yang diambil dan dikeringakan, kemudian diekstrak dengan pelarut etanol 96%. Dari hasil penelitian diketahui bahwa sediaan yang dapat terwarnai dengan baik pada konsentrasi ekstrak 0,5 gr/ml yang ditambahkan HCl 1% dengan waktu perendaman 30 menit. Serta pada konsntrasi ektrak 0,7 gr/ml dengan waktu perendaman 15 menit sel dapat terwarnai dengan cukup baik.
STUDI KOMPARATIF FRAKSI AIR SUSU IBU PERAH SEGAR DAN BEKU DALAM MENGHAMBAT BAKTERI ENTEROTOKSIGENIK Escherichia coli Andita Ayu Mandasari; Intan Chairun Nisa
Jurnal Pangan dan Agroindustri Vol. 7 No. 3 (2019)
Publisher : Jurusan Teknologi Hasil Pertanian, Fakultas Teknologi Pertanian, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jpa.2019.007.03.1

Abstract

Sumber nutrisi utama untuk pertumbuhan dan proteksi bayi yang mengandung makronutrisi, mikronutrisi, dan senyawa bioaktif adalah air susu ibu (ASI). Kegagalan menyusui dialami lebih banyak oleh ibu yang bekerja. Saat ini, pemberian ASI perah beku merupakan solusi alternatif tetapi kemampuan senyawa imunologis ASI perah beku dalam menghambat bakteri penyebab diare mungkin mengalami penurunan. Tujuan penelitian ini adalah membandingkan dan mengetahui kemampuan fraksi ASI segar dan beku dalam menghambat pertumbuhan bakteri Enterotoksigenik Escherichia coli (ETEC). Penelitian ini menggunakan quasi-experimental design dengan tujuh sampel ASI dengan kondisi empat belas hari pasca melahirkan dan disentrifugasi untuk mendapatkan fraksi supernatan dan pelet. Hasil penelitian ini adalah ada penurunan yang signifikan pada whole milk, supernatan, dan pelet ASI segar maupun beku dalam menghambat pertumbuhan ETEC. Fraksi supernatan pada ASI segar dan beku memiliki kemampuan lebih tinggi dalam menghambat bakteri dibandingkan dengan whole milk dan fraksi pelet. 
EVALUASI HAPUSAN DARAH TEPI DARAH K3EDTA SEGERA DI PERIKSA DAN DI TUNDA 24 JAM DALAM SUHU 2-8C Novi Ersanto; Adinda Dika Infantria; Titik Sundari; Andita Ayu Mandasari; Siti Nur Husnul Yusmiati
Jurnal SainHealth Vol 7, No 2 (2023): September 2023
Publisher : Faculty of Health Sciences Universitas Maarif Hasyim Latif

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51804/jsh.v7i2.14357.79-84

Abstract

Since specimen collection, preanalytical factors such as time and temperature could affect the peripheral blood smear morphology of erythrocytes, leukocytes, and thrombocytes. Delays are often caused by the limited number of medical personnel so work is not properly organized. This research is an observational qualitative descriptive study to determine the morphology of blood smears immediately checked and delay 24 hours at 2-8 ?. This study aims to determine the evaluation of K3EDTA venous peripheral blood smears immediately checked and delayed 24 hours at 2-8?. From 25 K3EDTA blood samples of Maarif Hasyim Latif Sidoarjo University students, it was found that there were no changes in the morphology of erythrocytes and platelets. Meanwhile, in leukocyte cells, changes occur in the neutrophil segment and stab granules, which is the granules fade, and the leukocyte nuclei can still be identified properly, so that do not affect the examination results. In conclusion, the Evaluation of the K3EDTA Peripheral Blood Smear was immediately checked and delay 24 Hours at the temperature of 2-8 ? has no change, which is still within normal limits. Keywords : Blood Smear, Delayed, K3EDTA, Immediately checked
EVALUASI HAPUSAN DARAH TEPI DARAH K3EDTA SEGERA DI PERIKSA DAN DI TUNDA 24 JAM DALAM SUHU 2-8C Novi Ersanto; Adinda Dika Infantria; Titik Sundari; Andita Ayu Mandasari; Siti Nur Husnul Yusmiati
Jurnal SainHealth Vol. 7 No. 2 (2023): September 2023
Publisher : Faculty of Health Sciences Universitas Maarif Hasyim Latif

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51804/jsh.v7i2.14357.79-84

Abstract

Since specimen collection, preanalytical factors such as time and temperature could affect the peripheral blood smear morphology of erythrocytes, leukocytes, and thrombocytes. Delays are often caused by the limited number of medical personnel so work is not properly organized. This research is an observational qualitative descriptive study to determine the morphology of blood smears immediately checked and delay 24 hours at 2-8 ?. This study aims to determine the evaluation of K3EDTA venous peripheral blood smears immediately checked and delayed 24 hours at 2-8?. From 25 K3EDTA blood samples of Maarif Hasyim Latif Sidoarjo University students, it was found that there were no changes in the morphology of erythrocytes and platelets. Meanwhile, in leukocyte cells, changes occur in the neutrophil segment and stab granules, which is the granules fade, and the leukocyte nuclei can still be identified properly, so that do not affect the examination results. In conclusion, the Evaluation of the K3EDTA Peripheral Blood Smear was immediately checked and delay 24 Hours at the temperature of 2-8 ? has no change, which is still within normal limits. Keywords : Blood Smear, Delayed, K3EDTA, Immediately checked
Co-Authors Adinda Dika Infantria Intan Chairun Nisa Kurnia Lintang Lukmatul Mutoharoh Novi Ersanto Setyo Dwi Santoso Siti Nur Asiyah Siti Nur Husnul Yusmiati Titik Sundari