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Uji Formulasi Pupuk Hayati Padat Berbasis Azolla Terhadap Populasi dan Fungsional Mikroba Tanah Menguntungkan Mieke Rochimi Setiawati; Maya Damayanti; Diyan Herdiyantoro; Pujawati Suryatmana; Fiqriah Hanum Khumairah
Soilrens Vol 15, No 2 (2017)
Publisher : Fakultas Pertanian Universitas Padjadjaran

Inorganic fertilizer is a very important agricultural input to increase agricultural production. Uncalculated and continuous application of inorganic fertilizers will lead to the reduction of soil fertility and degradation of environmental quality. Therefore, a strategy is needed to support the environmental sustainability in agriculture. One of the efforts is through the production of biofertilizer and organic fertilizer from local resources. Organic fertilizer for rice plants can be made by utilizing the local water fern, Azolla pinnata. A. pinnata can be applied on rice fields as green manure by directly placing it into the soil or spread on the surface of rice fields. Organic fertilizer that made from Azolla will be more advantageous because it can be applied with biofertilizer. This study aimed to determine the formulation of Azolla based solid biofertilizer that can support the growth of functional microbial population based on the Agriculture Ministry (Permentan) standard number 70/2011. The design of the study method used was Completely Randomized Design with six replications. The treatments used were inoculant starter with 3% molasses, 3% molasses + 10% rice wash water, 3% molasses + 1% yeast extract, and 3% molasses + 10% rice wash water + 1% yeast extract. The results of this study showed that Azolla-based solids fertilizer with starter formula 3% inoculant molasses + 10% rice water + 1% yeast extract have the population of Azotobacter, Azospirillum, endophytic bacteria and phosphate solubilizing microbe were greater than 108 cfu/g dry weight. Functional microbial of solids fertilizers in all formulas used do not show the difference on inhabiting in the N-free media or Pikovskaya selective media. Keywords: Azolla pinnata, formulation, biofertilizer, organic fertilize

Potensi Inokulan Petrofilik dan Kompos Kotoran Ayam dalam Bioremediasi Limbah Minyak Bumi Sistem Land Treatment Pujawati Suryatmana; Richard A. Gunawan; Diyan Herdiyantoro; Mieke Rochimi Setiawati
Soilrens Vol 17, No 1 (2019)
Publisher : Fakultas Pertanian Universitas Padjadjaran

Bioremediation of petroleum waste using the land treatment system is generally carried out by indigenous Petrophilic stimulation as a degrading agent. One of the efforts to improve the performance of the bioremediation system is by adding petrophilic inoculants and organic material as a source of nutrition. This experiment focused on observation of the effect of endogenous petrophilic consortium by adding chicken manure compost towards bioremediation performance parameters, including the efficiency of hydrocarbon degradation, soil pH and petrobacter population on Jatinangor Inceptisols contaminated with petroleum waste. The experiment Design used in this experiment was Factorial Randomized Block Design (FRBD). The first factor was the variation of the concentration of the petrophilic consortium, consisted of: (i) without a petrophilic consortium, (ii) the concentration of petrophilic consortium 1% (w/ w), (iii) the concentration of petrophilic consortium 3% (w/ w ), (iv) concentration of petrophilic consortium 5% (w/ w). The second factor was the variation of the concentration of chicken manure compost: (ii) without compost chicken manure, (ii) the concentration of chicken manure compost 1.5% (w / w), and (iii) the concentration of chicken manure compost 3% (w/w). ). The results showed that the treatment of exogenous Petrophilic and chicken manure could not significantly improve the biodegradation efficiency of hydrocarbons, but the Petrophilic additives of 3% (w/ w) could increase the highest Petrophilic population. From this study was found that indigenous petrophilic had the potential to degrade hydrocarbons by 71.20%, while the application of exogenous 1% (w/w) petrophilic was able to degrade hydrocarbons by 71.97 %.

Peningkatan Produktivitas Padi Gogo (Oryza Sativa Linnaeus) Akibat Aplikasi Pupuk Urea Dan Bakteri Pemfiksasi Nitrogen Dalam Bahan Pembawa Pada Inceptisols Asal Jatinangor Fadhilla Oktavianingtyas Trisilvi; Nadia Nuraniya Kamaluddin; Betty Natalie Fitriatin; Diyan Herdiyantoro; Pujawati Suryatmana
Soilrens Vol 19, No 2 (2021)
Publisher : Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/soilrens.v19i2.38360

As a staple in Indonesian diet, rice demand increase along with the population growth. Therefore, in order to fulfill the continuous increase, researches were conducted to increase land productivity. The increase of growth and productivity of upland rice with the application of Azospirillum sp. and Azotobacter sp. in compost, Azolla, and rice bran carrier were studied. Nitrogen fixer were applied as a biofertiliser as a mean to supply available nitrogen for upland rice growth. This research was conducted in Ciparanje Experimental Field and Soil Biology Laboratory, Faculty of Agriculture, Universitas Padjadjaran. In this research, Randomized Block Design was used to analyze two factors: urea dose (100% and 50%) and Nfixer bacteria in various carriers (individual carrier and combination of liquid inoculant, green compost, Azolla compost, rice bran). The result showed an interaction between two factors towards the grain weight of upland rice. The application of 100% urea dose showed higher result compared to 50% urea dose on grain weight. N fixer bacteria combined with compost, Azolla, and rice bran carrier gave the highest result on the number of tillers, chlorophyll content, and grain weight.

Pemanfaatan Limbah Pertanian dalam Menurunkan Dosis Pupuk Anorganik, Meningkatkan Populasi Azospirillum sp. , Nitrogen tanah, Serapan Nitrogen, dan Hasil Jagung pada Inceptisols Jatinangor Mieke Rochimi Setiawati; Diyah Sri Utami; Reginawanti Hindersah; Diyan Herdiyantoro; Pujawati Suryatmana
Soilrens Vol 19, No 1 (2021)
Publisher : Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/soilrens.v19i1.35084

Balanced fertilizer is a solution to increase Inceptisols fertility and maize productivity. The aim of this study was to determine the effects and interactions between various fertilizers organic fom agriculture waste with inorganic fertilizers to increase the total population of Azospirillum sp., nitrogen uptake, soil nitrogen and the yield of maize (Zea mays) in Inceptisols Jatinangor. The research was conducted from July 2020 to January 2021 in the experimental screen house at Ciparanje, Agriculture Faculty, Universitas Padjadjaran, Jatinangor, Sumedang, West Java, 724 meters above the sea level. This research design used a randomized block design (RBD) factorial with 16 treatments and three replicates. The first factor was the type of organic fertilizer (O) of four levels: without organic fertilizer (control), 10 tons ha1 of rice straw compost, sugarcane bagasse compost, and oil palm empty fruit bunche compost. The second factor was the dosage of inorganic fertilizers (A) of four levels: without inorganic fertilizer (control), 50%, 75% and 100% dose recommendation inorganic fertilizer. The results showed that no interaction between organic fertilizers and inorganic fertilizers against all the parameters. The independent effect of organic fertilizers had a significant effect on the parameters of the total population of Azospirillum sp., nitrogen uptake and maize yields. The independent effect of inorganic fertilizers has a significant effect on nitrogen uptake and maize yields. Treatment of 10 ton ha-1 of oil palm empty bunche compost and inorganic fertilizer (50% recommendation dose) was the best treatment for dry shelled weight per maize plant on Inceptisols, equal to 88.52 g and 87.96 g each plants.

Analisis C, N, C/N Ratio Tanah dan Hasil Padi yang Diberi Pupuk Organik dan Pupuk Hayati Berbasis Azolla Pada Lahan Sawah Organik Mieke Rochimi Setiawati; Diyan Herdiyantoro; Maya Damayani; Pujawati Suryatmana
Soilrens Vol 16, No 2 (2018)
Publisher : Fakultas Pertanian Universitas Padjadjaran

ABSTRACT Organic farming, especially organic rice requires a continuing supply of organic fertilizer in large quantity as a source of nutrition for rice plants. In long-run, organic system is can retain higher soil organic carbon (SOC), partially compensates the negative impact by improving physical and biological properties, as well as nutrient retention capacity of the soil. Azolla pinnata is an organic nitrogen source rich in protein. The high nitrogen content in A. pinnata is related to the presence of nitrogen-fixing Anabaena azollae microsimbion in A. pinnata leaves. A. pinnata compost is an alternative for biofertilizer carrier that can be used in organic rice fields. The contribution of azolla-based organic farming for organic farmers is to decrease the dependency on the animal manure in cropping season. The field experiment comprised of conventional compost as control and combination of organic fertilizer and biofertilizer with Azolla carrier. Organic fertilizer were 5 ton/hectare (ton/ha), 7.5 ton/ha, and 10 ton/ha combined with 0, 10, 20 kg/ha biofertilizer with Azolla carrier with no addition of inorganic fertilizer. Complete randomized design were used in the experiment which consisted of three replications. This technology produced organic fertilizer better than conventional compost of rice straw and manure made by farmers. Organic fertilizer base with Azolla mixture of 7.5 ton ha-1 plus solid biofertilizer (10 kg/ha) can produce 6.58 ton/ha of dry harvested grain, higher than conventional compost treatment (control). The application of organic fertilizer base on azolla and solid biofertilizers gave the increasing yield prospect of organic rice in Cisayong, Tasikmalaya regency West Java.Keywords : Azolla pinata, carbon, nitrogen, organic farming, rice.

Reaksi Hipersensitif Daun Tembakau oleh Isolat Bakteri Pelarut Kalium pada Praformulasi Pupuk Hayati Diyan Herdiyantoro; Mieke Rochimi Setiawati; Tualar Simarmata
Soilrens Vol 20, No 2 (2022)
Publisher : Dept Ilmu Tanah & Sumberdaya Lahan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/soilrens.v20i2.45266

The fruitfulness of biofertilizer application in increasing plant growth and yield is when the formulation uses selected microbial isolates that do not cause disease to plants. Hypersensitivity test is carried out to ensure that the microbial isolates used in the formulation of biofertilizer are not pathogenic to plants. The purpose of this study was to determine the pathogenicity of three selected potassium solubilizing bacteria (KSB) isolates (BPK-DHJ3-3150[17], BPK-DHJ1-4125[2], and BPKDHJ2-5250[16]) isolated from the maize plant rhizosphere on Inceptisols Jatinangor. The research was conducted using a qualitative experimental method that obtained data in the form of visual photos. The three selected KSB isolates were inoculated on tobacco leaves and observed for necrosis symptoms. The experimental results showed that the three selected KSB isolates did not cause a positive hypersensitivity reaction in tobacco leaves, which was indicated by the absence of necrosis symptoms at the leaf location where the KSB isolates were inoculated. The three KSB isolates were nonpathogenic isolates to plants.

Pengaruh Pupuk Hayati Berbasis Azolla terhadap Nitrogen Tanah, Nitrogen Tanaman, Populasi Azotobacter, dan Hasil Tanaman Mentimun Pada Inceptisol Jatinangor Mieke Rochimi Setiawati; Evi Entang Fatimah; Diyan Herdiyantoro; Apong Sandrawati; Uum Umiyati; Pujawati Suryatmana
Soilrens Vol 20, No 2 (2022)
Publisher : Dept Ilmu Tanah & Sumberdaya Lahan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/soilrens.v20i2.45271

The low productivity of cucumber plants in Indonesia can be caused by several factors, one of which is fertilization. Fertilization on Inceptisol soil which has a low nitrogen content is needed to increase cucumber crop yields. The use of inorganic fertilizers is limited to avoid environmental pollution, so the use of biofertilizers can be an environmentally friendly option. The purpose of this study was to determine the effect of concentration and interval application of azolla extract based biofertilizer on the total soil N, plant N content, Azotobacter population and cucumber yield. The experiment was carried out from August to November 2021 at the Ciparanje Experimental Garden, Faculty of Agriculture, Padjadjaran University. This experiment used a Completely Randomized Block Design of a combination concentrations (0, 5, 10, 15, 20 mL.L-1) and time of application (1, 2, 3 times). The results showed that the application of azolla extract based biofertilizer with different concentrations and application times had a significant effect on the azotobacter population but had no significant effect on soil nitrogen levels, plant nitrogen concentrations, and cucumber yields. The addition of 20 mL/L 2 times application of azolla-based biofertilizer increased cucumber fruit weight by 35.97% compared to control but did not cause a significant increase in yield

Aplikasi Pupuk Organik Azolla dan Pupuk Hayati terhadap Kandungan N Tanaman, Serapan N Tanaman, dan Hasil Tanaman Padi Sawah Organik pada Inceptisols Jatinangor Mieke Rochimi Setiawati; Diyan Herdiyantoro; Pujawati Suryatmana
Soilrens Vol 21, No 1 (2023)
Publisher : Dept Ilmu Tanah & Sumberdaya Lahan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/soilrens.v21i1.49320

The need for adequate nutrition for organic rice cultivation is very important. It is necessary to look for various sources of nutrients apart from organic fertilizers from rice straw compost and manure. Azolla water ferns and solid or liquid biofertilizers shoud be used as an alternative in the way to increase plant nutrients in organic rice cultivation in paddy fields. This experiment aims to increase the yield of lowland rice plants which are cultivated organically for the first time through the application of organic fertilizer enriched with azolla and biofertilizers in the lowland rice fields of Inceptisols Jatinangor. The experiment was carried out at the Experimental Garden, Ciparanje, it’s belong to Faculty of Agriculture, Universitas Padjadjaran, Sumedang, West Java. The design used was a Randomized Block Design (RBD) with six treatments, namely organic fertilizer, organic fertilizer + Azolla, organic fertilizer + solid biofertilizer, organic fertilizer + Azolla + solid biofertilizer, organic fertilizer + liquid biofertilizer, organic fertilizer organic + Azolla + liquid biofertilizer. Each treatment repeated four times. The experimental results showed that the application of a combination of organic fertilizers and biofertilizers had an effect on lowland rice yields but had no significant effect on plant N content and N uptake of lowland rice plants in Inceptisols Jatinangor.

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